JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Zhiming Chen,Jiabin Chen,Haoming Bao,Kaitao Liu,Pan Zeng,Fan Yang,Wei Cao,Dongqi Yang,An Cao,Tao Zhang,Yue Li
Surface-enhanced Raman scattering (SERS) has been one of the efficient strategies for trace molecular analysis, but the traditional substrates with sharp tips cannot effectively detecting the molecular isomers. Here, we develop a facile way to fabricate large-area, reproducible Ag/Au nanocavity arrays by the combination of microsphere-templated soft lithography and self-assembly. Compared to the flat and convex substrate, the Ag/Au units arranged on the concave surface exhibit a high-density of hotspots via spatial light confinement and multi-reflection-enhanced electromagnetic coupling, which enables highly sensitive detection of probe molecules down to10 −12 M. Moreover, by integrating machine learning algorithms, the test set shows a coefficient of determination (R 2 ) of 0.9896, achieving advanced training performance for classifying four structurally analogous quinolone antibiotics (ciprofloxacin, enrofloxacin, danofloxacin, and enoxacin) with a quantitative prediction of 10 −10 M. This work demonstrates a robust pathway for the controllable fabrication of high-performance SERS substrates for the development of intelligent, quantitative, and on-site sensing platforms in complex practical scenarios. Download: Download high-res image (179KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2025
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IF 9.8
Guangxin Yang,Jingru Zhang,Junyu Zhang,Peng Wang,Wei Xia,Jun Wang,Xiaosheng Shen,Cong Kong
Antibiotic use in aquaculture leads residues in aquatic products, contributing to antimicrobial resistance and potential human toxicity. Complex sample matrices hinder detection. This study uses wolfberry biomass waste-derived biochar as a novel solid-phase extraction (SPE) sorbent to detect 26 antibiotic residues (14 fluoroquinolones, 12 sulfonamides) in aquatic products, effectively repurposing agricultural waste into a high-performance, eco-friendly material. The mesoporous structure of the biochar enables rapid diffusion of antibiotics into its interior, facilitating highly efficient adsorption. Adsorption kinetics follow a pseudo-first-order model, reaching equilibrium in 60 min. The superior adsorption performance enables the prepared biochar to serve as an SPE sorbent for drug residue analysis with stable performance over at least three reuse cycles. The SPE was used for real samples analysis based on HPLC-HRMS, and the detection limits was 1–4.5 μg·kg −1 , recoveries was 75–119 %. Validation against the national standard GB 31658.17–2021 confirms method practicality and reliability.
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MOLECULES
·
2022
·Vol. 27
·期 24
·8764
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IF 4.927
Panxue Wang,Li Wang,Cen Li,Xiang Li,Guoliang Li
Reliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid detection and quantification of enrofloxacin. In the presence of enrofloxacin, the conformational transform of aptamers took place, and the distance between ROX and Au NP increased, which resulted in a decrease in the SERS signal intensity of ROX. Meanwhile, the intensity of the SERS signal of 4-MBN was used as an internal standard. Reliable determination of enrofloxacin was realized using the ratio of the SERS signal intensities of ROX to 4-MBN. Under optimal conditions, the developed ratiometric SERS aptasensor provided a wide linear range from 5 nM to 1 µM, with a correlation coefficient (R2) of 0.98 and a limit of detection (LOD) of 0.12 nM (0.043 ppb). In addition, the developed ratiometric SERS aptasensor was successfully applied for the determination of enrofloxacin in fish and chicken meat, with recovery values of 93.6–112.0%. Therefore, the established ratiometric SERS aptasensor is sensitive, reliable, time-efficient, and has the potential to be applied in the on-site detection of enrofloxacin in complex matrices.Keywords:enrofloxacin;detection;SERS aptasensor;ratiometric;portable Raman spectrometer;food safety
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TALANTA
·
2023
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IF 6.1
Chang Liu,Hong Lin,Limin Cao,Kaiqiang Wang,Jianxin Sui
The third generation of genetic engineering antibodies, single-domain antibodies, have been widely reported as potential biomaterials in recognizing small molecular hazards. In this study, a shark-derived single-domain antibody was used as the recognition element for the first time to detect enrofloxacin (ENR), one of the most representative hazards in aquaculture. An ENR-specific clone named 2E6 was isolated by phage display technology. Experimental results proved that 2E6 ssdAb showed high affinity to ENR-PEI complete antigen, with the highest OD 450 value of 1.348 in binding ELISA . Through icELISA, it was determined that the IC 50 of 2E6 ssdAb to ENR was 19.230 ng/mL, while the IC 10 was 0.975 ng/mL, with rare recognition to other fluoroquinolones, which showed high sensitivity and specificity to ENR. The 2E6 ssdAb also performed excellently in fish matrix immunoassay . Results showed that the ENR-negative fish matrix did not seriously interfere with the recognition of 2E6 ssdAb to ENR-OVA, with the matrix index between 4.85% and 11.75%, while the results of icELISA in ENR-spiked fish matrix showed that 2E6 ssdAb could recognize the target ENR in different ENR-spiked concentrations of the fish matrix (10–1000 ng/mL), with the recovery between 89.30% and 126.38% and the RSD between 1.95% and 9.83%. This study broadens the application scenario of shark-derived single-domain antibodies as small molecule recognition biomaterials, providing a new recognition element on ENR detection for immunoassay.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
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IF 4.4
Ye Jiang,Xiaochan Wang,Guo Zhao,Yinyan Shi,Yao Wu
Antibiotic residues in aquaculture environments disrupt the ecosystem balance and pose a potential hazard to human health when entering the food chain. Therefore, ultra-sensitive detection of antibiotics is necessary. In this study, a multifunctional Fe 3 O 4 @mTiO 2 @Ag core–shell nanoparticle (NP), synthesized using a layer-by-layer method, was demonstrated to be useful as an enhanced substrate for in-situ surface-enhanced Raman spectroscopy (SERS) detection of various quinolone antibiotics in aqueous environments. The results showed that the minimum detectable concentrations of the six investigated antibiotics were 1 × 10 −9 mol/L (ciprofloxacin, danofloxacin, enoxacin, enrofloxacin, and norfloxacin) and 1 × 10 −8 mol/L (difloxacin hydrochloride) under the enrichment and enhancement of Fe 3 O 4 @mTiO 2 @Ag NPs. Additionally, there was a good quantitative relationship between the antibiotics concentrations and SERS peak intensities within a certain detection range. The results of the spiked assay of actual aquaculture water samples showed that the recoveries of the six antibiotics ranged from 82.9% to 113.5%, with relative standard deviations ranging from 1.71% to 7.24%. In addition, Fe 3 O 4 @mTiO 2 @Ag NPs achieved satisfactory results in assisting the photocatalytic degradation of antibiotics in aqueous environments. This provides a multifunctional solution for low concentration detection and efficient degradation of antibiotics in aquaculture water.
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ACS Applied Materials & Interfaces
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2020
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IF 8.758
Xiangning Han,Hong Lin,Limin Cao,Xiangfeng Chen,Luefeng Wang,Hongwei Zheng,Ziang Zhang,Tushar Ramesh Pavase,Sai Wang,Xun Sun,Jianxin Sui
Purification of antibodies has become a critical factor in antibody production. A high-purity specific antibody against antigens, especially small molecules, seems to be difficult to obtain, even with the help of a protein A affinity column, which is a conventional and broadly used ligand for the separation of antibody and non-antibody proteins. Therefore, it is urgent to develop a cheap, simple, efficient, and stable method to separate the specific antibody from other antibodies. In this study, to improve the sensitivity and accuracy of immunoassay results, enrofloxacin (ENR) was grafted onto polyethylenimine (PEI) by the abundant amino groups and then the whole ligand (ENR–PEI) was conjugated to CNBr–Sepharose 4B to prepare the affinity column for the purification of the specific antibody against ENR from polyclonal antibodies. Scanning electron microscopy and Fourier transform infrared spectroscopy verification showed that Sepharose 4B was successfully modified by ENR–PEI with excellent uniformity. The capacity of the prepared column could reach to 6.15 mg of specific antibody with high purity per milliliter resin due to the high coupling ratio (49.3:1) of ENR on PEI, and the IC50 value of the antibody after purification was 47.58 ng/mL with a lowest limit of detection (IC10) of 1.099 ng/mL—18 times lower than those of the antibody purified through the protein A column. All the results showed that this new kind of resin could be used as the potential ligand in the purification of the trace-specific antibody against antigens in complex mixtures with high efficiency and specificity.
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ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
Lin Li,Hongyu Zhang,Qian Zhang,Ting Wang,Xiaohong Hou
In this study, Zeolitic Imidazole Framework-8/cellulose aerogel (ZIF-8/CA) hybrid was successfully fabricated through a simple doping method and ZIF-8 acted as the major component for adsorption. In order to elucidate the adsorption mechanism deeply, molecular simulation was adopted to the expound the interaction modes between ZIF-8 and the fluoroquinolones (FQs). ZIF-8/CA was used as the adsorbent for semi-automated pipette tip solid phase extraction (PT-SPE). In combination with high performance liquid chromatography tandem fluorescence detector (HPLC-FLD), the established method was successfully employed to determine trace amount of FQs in water samples. Extraction parameters such as the content of ZIF-8, pH of sample solution, volume of sample, flow rate of sampling, type and volume of elution solvent were investigated. Under the optimized conditions, satisfactory linearity was achieved with the correlation coefficient (R 2 ) ranging from 0.9954 to 0.9992. The limits of detection were in the range of 0.337–1.707 ng L −1 . And the recoveries varied from 75.9% to 96.8% with RSD less than 8.0%. The established method was demonstrated to be sensitive, efficient and convenient.
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Analytical Methods
·
2021
·Vol. 13
·期 29
·3264-3273
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IF 2.896
Jia Xu,Cao Li,Xiao Li,Yuxiu Xiao
A novel extraction technique i.e. hydrophobic magnetic nanoparticle (MNP)-assisted in situ supramolecular solvent (SUPRAS) microextraction was proposed, and it was applied for the analysis of sulfonamides (SAs) and fluoroquinolones (FQs) in aqueous samples, coupled with high performance liquid chromatography-UV detection (HPLC-UV). In this extraction method, hexafluoroisopropanol-mediated salt-free catanionic surfactant based SUPRAS in situ microextraction was initially carried out; then, the SUPRAS was quickly adsorbed by the hydrophobic magnetic nanoparticles and gathered by an external magnetic field. This can greatly shorten the separation time and overcome the dependence on centrifugation, and also perform a secondary extraction of free analytes (not extracted by SUPRAS) from water samples. The magnetic separation ability of different hydrophobic MNPs was evaluated by adsorbing supramolecular aggregates from the water sample. The effective parameters affecting the extraction efficiency of the analytes were investigated and optimized using the one variable at a time method. About 3 min was required to realize the extraction of analytes with an enrichment factor (EF) of 12–53 for SAs and 79–118 for FQs. Compared with the centrifugation-assisted SUPRAS microextraction, the hydrophobic MNP-assisted SUPRAS microextraction obtained much better extraction and preconcentration efficiency. The proposed novel extraction method with HPLC-UV provided LODs of 0.21–0.76 ng mL−1 for SAs and 0.10–0.18 ng mL−1 for FQs. Good linearity was obtained with correlation coefficients ranging from 0.9962 to 0.9999. The intra- and inter-day recoveries of the target antibiotics were in the range of 92.0–111.3% with RSD% below 10.4%. The method was successfully applied to determine SAs and FQs in real water samples, such as lake water, river water, reservoir water, and wastewater.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Weie Wang,Qinqin Sang,Ming Yang,Juan Du,Libin Yang,Xin Jiang,Xiaoxia Han,Bing Zhao
Herein, a surface-enhanced Raman scattering (SERS) strategy based on semiconducting substrate was exploited for detection of several antibiotic residues both in ultrapure water system and in actual water system. The as-prepared Ag-TiO 2 (Ag synchronously deposited and doped TiO 2 ) nanoparticle SERS-active substrate can achieve high sensitive SERS detection for difloxacin hydrochloride, ciprofloxacin, enrofloxacin, danofloxacin and enoxacin (five widely used quinolone antibiotics) in actual water samples, and the detection limits are as low as 4.36 × 10 −12 , 7.08 × 10 −11 , 3.94 × 10 −11 , 3.16 × 10 −11 and 3.15 × 10 −10 mol/L, respectively. These detection limits are far below the maximum of residue limit (3.01 × 10 −7 mol/L) stipulated by the European Union. And, the desirable quantitative relationships can be obtained in a wide concentration range. The recoveries of five antibiotic residues from spiked actual water samples are found to be more than 80.8% with the relative standard deviations between 2.1% and 4.7%. Even, the proposed SERS method can accurately distinguish every antibiotic species from a mixed antibiotic residue sample with multiple antibiotics. And, Ag-TiO 2 nanoparticles can also serve as an efficient photocatalyst for photocatalytic degradation of these antibiotic residues, which provides a multi-functional platform for synchronous determination and degradation of antibiotic residues in real environment.
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FOOD CHEMISTRY
·
2017
·
IF 4.529
Jia Xu,Xiao Li,Cao Li,Jia Chen,Yuxiu Xiao
Coacervation and phase separation were first reported in salt-free catanionic surfactant aqueous systems based on lauric acid (LA) and dodecyltrimethylammonium hydroxide (DTAOH), using hexafluoroisopropanol (HFIP) as a coacervate-inducing agent. The liquid–liquid two-phase separation occurs over a wide of LA/DTAOH molar ratios (78:22–0:100 mol/mol) and total surfactant concentrations (5–200 mmol L −1 ) upon adding a small amount of HFIP (<10%, v/v). HFIP-induced salt-free LA/DTAOH catanionic surfactant system has much wider two-phase region than HFIP-induced salt-containing sodium laurate/dodecyltrimethylammonium bromide system. A HFIP-induced LA/DTAOH coacervate extraction method was established and coupled to high-performance liquid chromatography-ultraviolet detector (HPLC-UV) for determination of fluoroquinolones (rufloxacin, ciprofloxacin, danofloxacin, enrofloxacin) in milk. Detection limits are from 0.3 ng mL −1 to 1.4 ng mL −1 . Intra- and inter-day precisions (n = 6) are in range of 4.5–8.3% and 5.8–10.7%, respectively. Recoveries are from 87.8% to 109.0%. The method, HFIP-induced salt-free coacervate extraction with HPLC-UV, is suitable for detecting trace fluoroquinolones in milk.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruoqin Jia,Lei Jia,Xiaolei Zhao,Yuanyuan Huang,Lina Zhang,Dan Zhao,Jun Xu,Tongqian Zhao
Sensitive and selective detection of fluoroquinolones, especially from different sources, is challenging. This study reported an uncommon three-cage lanthanide metal-organic framework ( 1-Eu ) with a Eu 3+ cluster as its structural primitive using a C 2 -symmetric 5,5-(Pyrazine-2,6-diyl) diisophthalic acid ligand. The 1-Eu probe effectively detected four fluoroquinolones through distinct color changes and spectral emission bands, demonstrating excellent performance with low detection limits: moxifloxacin (LOD: 9.3 nM), danofloxacin (LOD: 33.7 nM), gatifloxacin (LOD: 67.9 nM), and ofloxacin (LOD: 238.6 nM). Mechanistic studies revealed that internal filtration and photoinduced electron transfer (a-PET) effects were key factors. Furthermore, 1-Eu was successfully used to detect fluoroquinolones in food samples. Additionally, portable paper-based sensors were developed to quickly semi-quantify analyte concentrations using a smartphone color recognition app, underscoring the practical potential of this probe. This study introduces a novel methodology for the identification and detection of fluoroquinolones to enhance food safety.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Wenrui Li,Yike Dang,Ruihong Wang,Weizhong Jin,Xiangli Chen,Yafei Zhai,Zhuoqun Su,Guoliang Li
Fluoroquinolones (FQs) are widely used to treat various animal diseases. The high residue of veterinary drugs in animal-origin food are new pollutants that seriously threaten human health. In this work, Fe 3 O 4 @LDH/ZIF was prepared by the in situ growth of zeolitic imidazolium skeletons (ZIFs) on Fe 3 O 4 @Zn/Al-layered double hydroxides (Fe 3 O 4 @Zn/Al-LDH), which was used as the adsorbent of FQs for magnetic solid phase extraction (MSPE). The prepared materials were characterized by using FT-IR, XRD, TEM, SEM, VSM, zeta, and nitrogen adsorption-desorption analyses. Under the optimized conditions, the limit of detections (LODs) of proposed methods for FQs is determined as low as 0.1–0.6 μg·kg −1 with good linearity (R 2 ≥0.9972). Furthermore, the MSPE approach has been effectively utilized to extract of FQs from a range of meat varieties including fish, pork, chicken, bullfrog, and beef. The recoveries from the spiked samples ranged from 86.5 % to 107 %, with relative standard deviations (RSD) below 8.8 %. These results demonstrated that the prepared nanoparticles hold unique advantages for trace FQs analysis in foodstuffs. This study provides a magnetic adsorbent for food sample pretreatment, which can rapidly enrich compounds similar in structure to fluoroquinolones.
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危险品化学品经营许可证（带存储）