Asian Journal of Pharmaceutical Sciences
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2016
·Vol. 12
·期 2
·202-208
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IF 0
Huiyan Shi,Chenzhi Hou,Liqiang Gu,Zhe Wei,Hang Xing,Meiyu Zhang,Shixiao Wang,Longshan Zhao,Kaishun Bi,Xiaohui Chen
The present study was designed to investigate the influence of the pretreatment of piperazine ferulate on pharmacokinetic parameters of methotrexate in methotrexate-induced renal injury rats. A simple and efficient high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) method was developed to determine methotrexate in rat plasma. Methotrexate and syringic acid (internal standard) were extracted from rat plasma samples by protein precipitation with acetonitrile. The analysis was performed on a CAPCELL PAK C 18 column (150 mm × 4.6 mm, 5 µm) with acetonitrile and 5 mmol/l ammonium acetate aqueous (10:90, v/v ). The linear range was 5.0 × 10 −2 to 100.0 µg/ml for methotrexate. Other parameters were all within the acceptance criteria. The validated method was successfully applied the pharmacokinetic study of methotrexate between two methotrexate treated groups (with and without the pretreatment of piperazine ferulate). Compared with the methotrexate treated alone group, the pharmacokinetic parameters in the methotrexate with the pretreatment of piperazine ferulate group showed significantly lower MRT (0-t) , MRT (0-∞) and T 1/2 . Results suggested that methotrexate can be rapidly eliminated, cleared or metabolized in rat blood, which might be related to the pretreatment of piperazine ferulate. The method provided deeper insights into rational clinical use of methotrexate with the pretreatment of piperazine ferulate on cancer patients with renal dysfunction.
研究领域:
药理学
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药物代谢
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肾脏病学
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肿瘤学
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临床药学
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分析化学
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CEREAL CHEMISTRY
·
2018
·Vol. 96
·期 1
·142-153
·
IF 1.138
Binle Zhang,Yang Wendan,Feng Wang,Jacob Ojobi Omedi,Ruoshi Liu,Jinxin Huang,Luan Zhang,Qibo Zou,Weining Huang,Shaolei Li
Background and objectives In this study, Kluyveromyces marxianus with ability to produce β-glucosidase was used to preferment wheat bran (WB) and release a mixture of natural enzymes for improving dough performance and bread quality. Five different enzyme activities were analyzed. The effect of the prefermented WB with enzyme mixture on stickiness and microstructure of dough, ferulic acid release in bread, and molecular weight distribution of arabinoxylan were evaluated using Texture Analyzer, scanning electron microscopy (SEM), HPLC, and high-performance gel filtration chromatography (HPGFC). Findings Kluyveromyces marxianus exhibited abundant extracellular β-glucosidase activity (up to 6.98 U/g). The main enzymes released in prefermented bran were endoglucanase and exoglucanase, β-glucosidase, xylanase, and ferulate esterases. These enzymes were active during bread making (only prefermented bran added as improver or combined with xylanase) and affected solubility and molecular weight distribution of dietary fiber, which resulted in positive improvement of dough performance, release of phenolic acid, and overall quality of the breads. The changes observed were attributed to the extracellular enzymes from K. marxianus and endogenous enzymes activated during prefermentation, thus enhancing the WB functionality in bread. Conclusions Wheat bran bioprocessing using high enzyme producing yeasts increases functional application of wheat bran in baked products. Significance and novelty This study added new knowledge for developing a natural enzyme-based improver for bran-enriched baked products in the food industry.
研究领域:
食品科学
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应用微生物学
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生物加工技术
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谷物化学
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功能食品
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高影响因子
Journal of Food Measurement and Characterization
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2026
·
IF 3.6
Chen Linlin,Yu Xiao,Li Wei,Zhang Haipeng,Zhang Sicong,Xin Jiaying,Zhang Na
Ferulic acid exists in nature mainly in the bound state in plant cell walls and is found in grains, fruits, vegetables, and Chinese herb.FA has a wide range of biological activities. However, the low stability and water solubility of FA limit its bioavailability. This has led to the encapsulation of FA to improve its bioavailability. This study prepared the binary polysaccharide-based carrier β-cyclodextrin/debranched rice starch (β-CD/DRS) by ultrasonic self-assembly. The particle size of β-CD/DRS was 107.23 ± 2.14 nm with a PDI index of 0.311 ± 0.075, which is affected by ultrasound time. The ternary inclusion complex of FA@β-cyclodextrin/debranched starch (FA@β-CD/DRS) was synthesized by the co-deposition method; the encapsulation and loading rate of FA were 89.32 ± 2.53% and 7.48 ± 0.54%, respectively. The structures of the ternary inclusions were analyzed by SEM, FT-IR, XRD, and DSC. The β-CD/DRS assembled using different ultrasound times modulated the FA@β-CD/DRS properties after deposition. The results showed that the inclusion compound was 1.22 times more photostable and 1.44 times more thermostable than FA. In the simulated gastrointestinal tract, the optimal in vitro release kinetics model was a first-order kinetic model, which was affected by pH. At pH 8.0, the cumulative release of ferulic acid was higher. The inclusion complex can continuously release ferulic acid, achieving a sustained-release effect, and its solubility in water is 3.9 mg/mL, which is approximately six times that of ferulic acid. In summary, the use of binary polysaccharide-based vector encapsulation can provide a more efficient method for the stabilization and delivery of phenolic acids. Graphical
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Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121185
·
IF 7.2
Peng Gao,Li Liu,Guirong Bao,Dequan Chen,Jia Luo,Xuewu Ji,Wenyao Deng
The conversion of furfural (FF) to cyclopentanone (CPO) via selective hydrogenation-rearrangement represents a crucial route for sustainable, high-value valorization of renewable biomass feedstocks. In this study, carbon dots (CDs) were utilized as both a reducing and a dispersing agent for the one-pot green photoreduction synthesis of PdAg bimetallic catalysts supported on CDs (Pd x Ag y /CDs) in aqueous phase. Among the catalysts with varying Pd-to-Ag molar ratios, the Pd 0.5 Ag 1 /CDs catalyst demonstrated exceptional performance, achieving 100 % FF conversion and 93.3 % CPO selectivity within 4 h at 200 °C. The superior activity is attributed to a synergistic effect between the acid sites on the CDs and the PdAg alloy, which facilitates the rearrangement of the intermediate furfuryl alcohol (FA). Mechanistic studies, including calculations of the free energy barriers for elementary steps from FA to 4-hydroxy-2-cyclopentenone, revealed a ring-opening pathway for the furan methyl carbocation, which dramatically reduces the reaction energy barrier to 47.1 kJ/mol from 195.8 kJ/mol of the Piancatelli pathway. This work not only presents an efficient and green catalyst for FF upgrading but also highlights the promising application of CDs in advanced catalytic systems for circular economy-driven chemical production.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Ying He,Zhenan Rao,Ziang Tian,Xiaojuan Lei,Jichun Zhao,Lin Lei,Jian Ming
Morchella esculenta protein was evaluated as a fungal alternative protein ingredient. • Ferulic and chlorogenic acids modulated MP structure in a concentration-dependent manner. • Moderate phenolic acid functionalization improved emulsifying, foaming, and digestibility. • Excessive phenolic acid addition induced aggregation and reduced colloidal stability. • Structure-dependent phenolic functionalization offers a strategy for alternative-protein foods. Fungal proteins face poor processing functionality and low digestibility limiting their use in emulsified and structured foods. Here, Morchella esculenta protein (MP) was modified by ferulic acid (FA) and chlorogenic acid (CA) at 0.0375–1 mg/mL. Both phenolics bound MP concentration-dependently, CA exhibited stronger binding affinity ( K a = 3.242 × 10 6 L/Mol) and deeper conformational rearrangement, while FA relied on hydrophobic and π–π interactions. Moderate phenolic addition maximized MP performance: EAI rose from 2.44 to 3.99 m 2 /g and digestibility from 45.33% to 58.31%, with CA delivering superior antioxidant capacity (DPPH scavenging 69.67%). Excess phenolics triggered severe protein aggregation and weakened interfacial stability. This work clarifies structure-dependent differential regulation of MP by two phenolics, defines optimal concentration thresholds, and provides theoretical support for clean-label fungal protein food ingredients Download: Download high-res image (139KB) Download: Download full-size image
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高影响因子
FOOD CHEMISTRY
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2026
·
IF 10.4
Meigui Huang,Ruifeng Ying,Mehraj Ahmad,Qiuhong Liao,Yonghong Li
The ultrasonic treatment was more likely to form V-shaped complexes. • UT enhanced the thermal stability of the complex. • UT significantly enhanced the anti-digestive property of the complex. This study aimed to investigate the effects of complexes formed by ultrasonicated highland barley starch (UHS) and ferulic acid (FA) at concentrations of 1% to 5% on the functional properties and digestive characteristics of starch. The XRD results showed that UHS formed more V-shaped crystal structures with FA, and the maximum amount of FA increased from 3% to 5%. Fourier transform infrared spectroscopy demonstrated that it enhanced the crystallization and hydrogen bond interaction of UHS with FA. The 1 H NMR spectrum of the complex revealed a more pronounced low-field shift, confirming that ultrasonic treatment significantly enhanced the interaction between starch and FA. This results in higher crystallinity, more complex structural density, and superior viscoelastic and thermal properties, enhancing anti-digestibility and increasing anti-starch content from 20.69% to 28.22%. UT effectively modulates the complex's physicochemical and digestive properties, suggesting potential applications in functional highland food development. Schematic diagram of the experimental procedure. Download: Download high-res image (263KB) Download: Download full-size image
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PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yuhang Xin,Yingxi Dong,Zhuoer Tian,Xiaoru Wang,Xuebo Liu,Xiang Duan
Polyphenols alleviate fructose-induced lactoferrin glycation and restore its digestibility. • Ferulic acid increases lactoferrin's hydrolysis degree to 68.6% after gastrointestinal digestion. • Gallic acid and epicatechin promote production of small peptides (<3 kDa) from glycated lactoferrin. • Polyphenols protect lysine/arginine residues from glycation, preserving protease cleavage sites. • First structure-dependent comparison of three polyphenols in improving glycated protein digestion. This study addressed issues such as protein aggregation, reduced digestibility, decreased nutritional bioavailability, and the formation of harmful advanced glycation end products (AGEs) caused by fructose-induced glycation of bovine lactoferrin during thermal processing. We investigated the inhibitory effects of three pineapple peel polyphenols (gallic acid, epicatechin, and ferulic acid) on lactoferrin glycation, as well as their regulatory roles in the in vitro digestibility, antioxidant activity, and AGE formation of this protein. The results indicate that the three polyphenols inhibit lactoferrin glycation and aggregation in a structure-dependent manner, significantly improving its gastrointestinal digestibility (ferulic acid increased the degree of hydrolysis at the end of digestion to 68.6%), enhancing the antioxidant capacity of the digestion products, and effectively suppressing AGE formation during digestion. This study provides theoretical support for the application of representative pineapple peel polyphenols as glycation inhibitors to improve the nutritional utilization of milk proteins. Download: Download high-res image (165KB) Download: Download full-size image
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高影响因子
Agronomy-Basel
·
2025
·Vol. 15
·期 6
·1428
·
IF 3.4
Liang Hu,Dabing Yang,Hongbo Wang,Xueshu Du,Yan Wu,Liang Lv,Tongmin Mou,Aiqing You,Jinbo Li
Rice, a vital crop, faces significant threats from the brown planthopper (BPH), which impacts plant growth and yield. Pyramiding the BPH resistance genesBPH14andBPH15provides rice crops with reliable and lasting protection against BPH. Nonetheless, current research lacks clarity on the molecular processes responsible forBPH14/BPH15-mediated resistance to BPH. In this study, utilizing high-throughput metabolomics and integrating transcriptomic data, we investigated the metabolic adaptations of theBPH14/BPH15pyramiding line (B1415) and its recurrent parent (RP) during early and late infestation stages. The analysis identified 1007 metabolites, mainly consisting of lipids and lipid-like molecules, together with phenylpropanoid and polyketide classes. Differentially accumulated metabolites (DAMs) displayed different patterns in B1415 and RP, particularly in flavonoid and phenylpropanoid biosynthesis pathways, which were more pronounced in the resistant B1415. Furthermore, ferulic acid (FA) was found to negatively regulate BPH resistance. These findings elucidate critical metabolic pathways involved in rice defense mechanisms and underscore the potential of B1415’s enhanced metabolic responses in conferring durable resistance against BPH.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Han Wang,Lu Chen,Shuang Wu,Weiping Jin,Wangyang Shen,Zhongze Hu,Wenjing Huang,Gang Liu
Rice bran oil bodies (RBOBs) are one of the most exploited functional components from rice bran by-products and are predominantly based on oleosin stabilization. In this study, we explored the effects of different concentrations of added (-)-epicatechin, ferulic acid, and phytic acid on the RBOBs stability. The results revealed that the incorporation of all three natural phytoconstituents could reduce the RBOBs particle size and increase emulsifying properties, demonstrating increasing surface hydrophobicity (p < 0.05), and a good antioxidant effect, which was especially obvious with (-)-epicatechin incorporation. Fourier transform infrared (FT-IR) spectroscopy data demonstrated that these three small molecule substance classes can modify with oleosin on RBOBs surface by covalent and noncovalent effects. Raman spectroscopic analysis illustrated that the vibrational modes of disulphide bonds in oleosin were modified by these three plant natural ingredients. The interactions between the three phytoconstituents and the model protein were investigated by molecular docking experiments.
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PubMed
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Shi Xia,Fang Jiaxing,Du Huicong,Zhang Sufang,Liu Fu,Zhang Zhen,Kong Xiangbo
When Ips bark beetles invade and colonize the host plants, their associated pathogenic fungal partners are carried into the phloem of the host trees. Host trees are lethally attacked by the beetle-fungus complex and the collective damage severely limits forestry production worldwide. It is of great importance to verify whether bark beetles and their associated fungi show concordant performance in terms of biology, physiology, and biochemistry on host trees. In this study, the two Ips bark beetles Ips typographus and Ips subelongatus (Coleoptera: Curculionidae, Scolytinae), their respective associated pathogenic fungi Endoconidiophora polonica and Endoconidiophora fujiensis, and their respective host plants Picea jezoensis and Larix olgensis were selected as test material. Cross-inoculation experiments were conducted indoors and outdoors to investigate the differences in reproduction and development of two beetles and infectivity of two fungi on two plants, as well as the differences in physiological responses of two plants to two fungal infections. The results showed that I. typographus and E. polonica had excellent host performance on P. jezoensis; however, neither successfully colonized and infected L. olgensis. In contrast, I. subelongatus and E. fujiensis showed strong host suitability on L. olgensis and some degree of suitability on P. jezoensis, although the host suitability of P. jezoensis for E. polonica was significantly higher than that for E. fujiensis. In addition, we found that the absolute amount of ergosterol accumulated on the lesion was positively correlated with lesion area. The ergosterol amount and lesion area were both strongly correlated with the release of host monoterpenes, but had no obvious correlation with the concentration of fungi-induced phenols on the lesion area and the side-chain oxidation of lignin in the xylem of the infected sites. Based on these results, we confirmed that “I. typographus-E. polonica” and “I. subelongatus-E. fujiensis” complexes both showed the most suitable consistent performances on their own traditional hosts, establishing a stable species-specific association relationship in these two beetle-fungus complexes, with the “I. subelongatus-E. fujiensis” complex showing broader host suitability. From the perspective of physiological responses of plants to fungal infections, monoterpenes are an important indicator of host suitability.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Shuohan Ma,Qingqing Zhu,Siyu Yao,Ruihao Niu,Yu Liu,Yu Qin,Yuxue Zheng,Jinhu Tian,Dandan Li,Wenjun Wang,Donghong Liu,Enbo Xu
The starch–phenolics complexes are widely fabricated as functional foods but with low phenolics retention limited by traditional liquid reaction and washing systems. In this study, ferulic acid (FA, 5%) was exogenously used in the crystalline form, and it reacted with starch in a high-solid extrusion environment, which was simultaneously controlled by thermostable α-amylase (0–252 U/g). Moderate enzymolysis (21 or 63 U/g) decreased the degree of the starch double helix and significantly increased the FA retention rate (>80%) with good melting and distribution. Although there were no significantly strong chemical bonds (with only 0.17–2.39% FA bound to starch hydrolysate), the noncovalent interactions, mainly hydrogen bonds, van der Waals forces, and electrostatic interactions, were determined by 1H NMR and molecular dynamics simulation analyses. The phased release of total FA (>50% in the stomach and ∼100% in the intestines) from bioextrudate under in vitro digestion conditions was promoted, which gives a perspective for handing large loads of FA and other phenolics based on starch carrier.
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PubMed
高影响因子
Frontiers in Microbiology
·
2023
·
IF 5.2
Song Wei,Xin Jiaying,Yu Chong,Xia Chungu,Pan Yu
Ferulic acid (FA) is a natural antibacterial agent rich in plants, FA has excellent antioxidant and antibacterial properties. However, because of its short alkane chain and large polarity, FA is difficult to penetrate the soluble lipid bilayer in the biofilm to enter the cell to play an inhibitory role, limiting its biological activity. To improve the antibacterial activity of FA, with the catalytic condition of Novozym 435, four alkyl ferulic acid esters (FCs) with different alkyl chain lengths were obtained by fatty alcohols (including 1-propanol (C3), 1-hexanol (C6), nonanol (C9), and lauryl alcohol (C12)) modification. The effect of FCs on P. aeruginosa was determined by Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), Growth curves, alkaline phosphatase (AKP) activity, crystal violet method, scanning electron microscopy (SEM), membrane potential, PI, cell contents leakage. Results showed that the antibacterial activity of FCs increased after esterification, and the antibacterial activity significantly increased and then decreased with the extension of the alkyl chain of the FCs. Hexyl ferulate (FC6) showed the best antibacterial activities against E. coli and P. aeruginosa (MIC for E. coli was 0.5 mg/ml, MIC for P. aeruginosa was 0.4 mg/ml). And Propyl ferulate (FC3) and FC6 showed the best antibacterial activities S. aureus and B. subtilis (MIC for S. aureus was 0.4 mg/ml, The MIC of B. subtilis was 1.1 mg/ml). In addition, the growth, AKP activity, bacterial biofilm, bacterial cell morphology, membrane potential and cell contents leakage of P. aeruginosa after different FCs were investigated, which found that FCs could damage the cell wall of P. aeruginosa and showed different effects on the P. aeruginosa cell biofilm. FC6 showed the best inhibition on the biofilm formation of P. aeruginosa cells, which caused the surface of P. aeruginosa cells to be rough and wrinkled. Some P. aeruginosa cells showed aggregation and adhesion, even rupture. The membrane hyperpolarization was obvious, which appeared as holes, leading to cell contents leakage (protein and nucleic acid). All these results concluded that the antibacterial activities FCs against foodborne pathogens depended on different fatty alcohol esterification of FA. FC6 showed the best inhibition on P. aeruginosa due to its effect on P. aeruginosa cell walls and biofilms and the leak of the cell contents. This study provides more practical methods and a theoretical basis for giving full play to the bacteriostatic effect of plant FA.
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高影响因子
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