ACS Omega
·
2018
·
IF 0
Feng Pan,Yilin Zhang,Zhiqin Yuan,Chao Lu
Carminic acid (CA) extracted from cochineal is widely used in food additives as a natural colorant, and its potential risk to human health makes its detection important. In this work, a layered doubled hydroxide (LDH)–luminol–H2O2 system-based chemiluminescence (CL) platform has been successfully applied for CA sensing. The principle detection consists of two steps: first, LDH adsorbs CA onto the surface via electrostatic attraction; second, CA quenches the CL of the LDH–luminol–H2O2 system via the synergistic effect of CL resonance energy transfer, reduction of reactive oxygen species, and occupation of positively charged centers of brucite-like layers. With this CL approach, 0.5 μM CA is detectable using a CL spectrometer, and the limit of detection is 0.03 μM. This CL system exhibited a linear response to CA in the concentration range from 0.5 to 10 μM. In addition, the practical application of the designed CL sensing system is evaluated with dried pork slice samples.
研究领域:
分析化学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Yan Jiang,Tongtong Zhang,Qiong Wu,Jingwen Zhao,Fengna Xi,Jianmin Wu
Developing highly sensitive electrochemiluminescence (ECL) systems is crucial for the analysis of tumor biomarkers. Herein, mildly oxidized porous silicon (MOPSi) was firstly employed to immobilize cerium oxide (CeO 2 ) nanozymes to amplify the ECL response of the luminol-dissolved oxygen (DO) system in a near-neutral condition for Alpha-fetoprotein (AFP) immunoassay. MOPSi nanoparticles possess a unique Si core/SiOₓ shell structure that enables the stable anchoring and dispersion of CeO 2 via Ce-O-Si bonds. Notably, CeO 2 -MOPSi/ITO exhibits a markedly higher ECL intensity than CeO 2 /ITO and MOPSi/ITO owing to the robust heterostructure formed between CeO 2 and MOPSi. The CeO 2 -MOPSi heterointerface enables a self-cascade catalytic process that comprises efficient oxygen reduction to produce superoxide anion radicals (O 2 •- ) and H 2 O 2 , followed by the in-situ conversion of the generated H 2 O 2 into additional O 2 •- . The abundant O 2 •- reacts with luminol radical anion (L •- ), leading to an enhanced ECL response. Moreover, an AFP immunosensor was fabricated by anchoring antibodies onto MOPSi surface. The proposed sensor shows a broad linear range from 10 fg/mL to 10 ng/mL with a detection limit of 3.3 fg/mL. This work provides a novel strategy for integrating the MOPSi carrier with nanozymes to develop advanced ECL systems for high-performance bioanalysis. Download: Download high-res image (123KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Sipei Gao,Zhe Jiang,Hao Hao,Zheng Li,Haijiao Xie,Wei Zhang
Natural colorants inhibit bloodstain spread on fabrics through a physical barrier. • Polyphenols inhibit hemoglobin release via fiber bonding and iron coordination. • Rutin quenches ·OH and luminol; alizarin only ·OH, giving weaker suppression. Cotton is a globally important economic and industrial crop. Cotton fiber serves as both the core raw material for textile processing and natural dyeing, and as a substrate for forensic bloodstain analysis. Luminol chemiluminescence is a key technique for bloodstain analysis. However, its performance is affected by carrier surface properties, especially interference from textiles dyed with natural colorants. Here, cotton fabrics were dyed with Sophora japonica and Rubia cordifolia . Combining surface characterization and chemiluminescence kinetics, a dual physical-chemical interference mechanism was revealed. The results revealed that physical inhibition, caused by a hydrophobic barrier, increased the blood contact angle by 2.32 times and reduced the spreading area by 45.1%. while chemical regulation, arising from polyphenol interactions, decreased hemoglobin release by 18.2% and quenched the luminescence intensity by 80%. Crucially, density functional theory (DFT) calculations elucidated the distinct molecular pathways of two representative components: rutin suppresses chemiluminescence via a dual pathway (scavenging both •OH and luminol radicals), whereas alizarin exhibits only weak inhibition through limited •OH scavenging. The physical barrier primarily restricts Hb accessibility, while the chemical scavenging and coordination predominantly govern the final luminescence signal loss. This study provides theoretical support for bloodstain detection and broader use of industrial crops in functional textiles. Download: Download high-res image (183KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Rongrong Pan,Limei Zhu,Xianzhu You,Rongjiao Sun,Yueqi Jin,Jiangqian Kong,Hua Zhou,Jiante Li,Ying Tan,Jiajia Dong,Wanle Hu
While oral administration offers distinct advantages for the treatment of ulcerative colitis (UC), its efficacy is often limited by poor drug targeting and the disease’s multifactorial pathology. Herein, we report an orally bioavailable nanotherapeutic system, CAB@Que, in which cellulose acetate butyrate (CAB) functions not merely as a delivery vehicle for quercetin (Que) but as a colon-specific butyrate prodrug, enabling synergistic and targeted therapy of UC. The CAB nanocarrier itself exhibits intrinsic therapeutic activity: upon reaching the inflamed colon, its butyryl side chains undergo hydrolysis triggered by colonic alkaline pH and microbial esterases to release butyrate, while residual polymer fragments are further fermented by commensal microbiota to generate additional short-chain fatty acids (SCFAs). This dual-source SCFA supplement actively supports epithelial energy metabolism and mucosal healing. Meanwhile, Que is coreleased to exert potent anti-inflammatory and antioxidant effects. In a murine colitis model, CAB@Que demonstrated superior efficacy over free Que, blank CAB, or their physical mixture, significantly ameliorating clinical symptoms, restoring epithelial barrier integrity, alleviating systemic oxidative stress, and re-establishing gut microbial homeostasis. By integrating carrier-mediated butyrate prodrug action with payload-driven immunomodulation, the CAB@Que platform enables precise, holistic intervention across the “microbiota–mucosa–immune” axis, offering a rationally designed oral strategy for synergistic UC therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2026
·
IF 3.6
Xianchuan Dong,Yaoxuan Wang,Jing Sui,Siqi Huang,Feifei Zhang,Zonghua Wang
A nickel-based multichannel bipolar electrochemiluminescence (BP-ECL) sensor was reported for the rapid and non-invasive detection of glucose in urine. The bipolar electrodes are fabricated using nickel, an inexpensive material with excellent catalytic activity, thereby reducing the cost of mass-scale device fabrication from the source. The detection mechanism relies on the glucose oxidase-mediated production of hydrogen peroxide, which proportionally enhances the luminol electrochemiluminescence (ECL) intensity. The optimized sensor exhibited a low detection limit of 0.1 μM and a linear response across the range of 1.0 to 500.0 μM, demonstrating excellent sensitivity and stability. The developed multichannel BP-ECL sensor can be powered with only two commercial alkaline batteries (~3.2 V) without the need for sophisticated instrumentation, highlighting its significant potential for point-of-care testing and home-based health monitoring. The overall system features low cost, simple operation, and fast response, offering a feasible technical solution for daily monitoring of chronic diseases and primary healthcare screening.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Jingming Zhai,Heying Li,Jianfeng Bao,Xiaozhi Zhang,Jinghua Li
Photodynamic therapy (PDT) represents an exceptionally hopeful approach for cancer therapy, with two primary factors influencing its therapeutic effectiveness are the light source and the oxygen source. Because of the shallow depth of tissue penetration by visible light and the low oxygen conditions present in the hypoxia tumor microenvironment, the therapeutic outcomes are often suboptimal. To tackle this issue, This study created an innovative photodynamic drug delivery system that simultaneously achieves self-luminescence and oxygen self-supply, thereby enhancing the PDT efficacy. The system PBNCs@Vc@Luminol in this study utilizes hollow Prussian blue nanocubes (PBNCs) as the main structure, loaded with the hydrogen peroxide trigger agent (vitamin C, Vc) and the intracellular chemiluminescent agent (luminol). PBNCs@Vc@Luminol nanoparticles demonstrate superior efficiency in converting near-infrared (NIR) light to heat for in vivo photothermal tumor therapy (PTT). Additionally, they can induce luminol chemiluminescence (CL) at the tumor site in situ, which is absorbed by PBNCs via chemiluminescence resonance energy transfer (CRET), sensitizing molecular oxygen (O 2 ) triggered by Vc to produce adequate reactive oxygen species (ROS) for PDT. Experimental studies conducted demonstrated that PBNCs@Vc@Luminol nanocomposites exhibit excellent anti-tumor effects both in vitro and in vivo , facilitating the combined effects of PTT and PDT with the assistance of magnetic resonance imaging (MRI), thereby achieving real-time monitoring and efficient treatment of tumors.
✓ 已验证
产品关联
DOI
高影响因子
LUMINESCENCE
·
2026
·Vol. 41
·期 7
·e70592
·
IF 2.8
Lanlan Wu,Xiaochun Deng,Jiyang Liu
The detection of metabolites, such as uric acid (UA), is crucial for diagnosing and monitoring various metabolic disorders. Herein, an electrochemiluminescence (ECL) sensor for uric acid (UA) detection was constructed via integrating a cascade of bioenzyme (urate oxidase [UOx]) and confined nanozyme reactions. Gold nanoparticles (AuNPs) were confined within amino‐functionalized vertical mesoporous silica film (NH 2 ‐VMSF) via electrochemical deposition. The NH 2 ‐VMSF was synthesized on indium tin oxide (ITO) through electrochemical‐assisted self‐assembly (EASA) method. Amino groups on the NH 2 ‐VMSF surface served as anchoring sites for AuNPs, which were electrochemically reduced and confined within the nanochannels. The confined AuNPs exhibited strong pseudo‐peroxidase‐like activity, boosting ECL response of luminol‐H 2 O 2 . Compared to the NH 2 ‐VMSF/ITO electrode, the AuNPs‐deposited electrode produced a 3.4‐fold increase in ECL intensity. Urate oxidase catalyzed the oxidation of uric acid, producing H 2 O 2 , which acted as a co‐reactant for luminol, enabling indirect uric acid detection. The sensor demonstrated a detection range of 0.1–20 μM with a low limit of detection (LOD, 85 nM). The sensor was used for UA detection in complex biological samples (urine). The nanoconfined AuNPs provided a stable and effective environment for nanozyme‐catalyzed reactions, ensuring high sensitivity and long‐term stability. A cascade electrochemiluminescence sensor integrating urate oxidase catalysis with nanochannel‐confined AuNPs nanozyme enables highly sensitive and selective detection of uric acid. The confined AuNPs exhibit strong peroxidase‐like activity, amplifying the luminol‐H 2 O 2 signal by 3.3‐fold and achieving a low limit of detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xiaoyu Qin,Wenhao Guo,Yining Wang,Xiangdong Cai,Jianxi Xiao
Pathological collagen emerges as a critical biomarker in the progression of tumors, fibrotic disorders, and osteoarthritis. The precise quantification of pathological collagen holds critical diagnostic value, offering a noninvasive approach for early detection of collagen-associated disorders. A pathological collagen targeting nanozyme biochip (PCC) has been constructed for the first time to ultrasensitively quantify pathological collagen in body fluids. The PCC biosensor consists of a high-affinity pathological collagen targeting nanozyme (Pacotzyme) and a capture surface for efficient enrichment of unbound Pacotzyme. The PCC has been demonstrated to quantitatively analyze pathological collagen across a wide concentration range from 0.1 to 10000 ng/mL, with an LOD as low as 15 pg/mL. PCC demonstrates robust and precise performance in intricate biological environments characterized by elevated protein content, high ionic strength, and increased glucose levels. Moreover, PCC exhibits consistent and reliable functionality in diverse body fluids, including serum, urine, saliva, and synovial fluid. Notably, PCC chips have been successfully applied to detect cancer, fibrosis, and osteoarthritis by quantifying pathological collagen in body fluids. The specific nanozyme biosensor offers a promising diagnostic tool for the early screening and noninvasive diagnosis of collagen-related diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 11
·1-10
·
IF 5.3
Chen Zixuan,Ma Shuhui,Wang Chujun,Liu Jie,Yang Xiaoyan
Monitoring of the amyloid-beta (A β ) peptide is crucial for the diagnosis and treatment of Alzheimer’s disease. Here, a label-free, signal-on ratiometric electrochemiluminescence (ECL) aptasensor was fabricated for the detection of A β peptide. Specifically, graphite-like carbon nitride (g-C 3 N 4 ) nanosheets and luminol served as the cathodic and anodic ECL emitters, respectively. K 2 S 2 O 8 and H 2 O 2 were used as the coreactants for the two emitters. Luminol was first coated on the indium tin oxide (ITO) electrode and used as the internal standard in the ratiometric biosensor. Polyaniline was then electrodeposited on the surface of the luminol-modified electrode to improve the biocompatibility and conductivity of the sensing interfaces. Gold nanoparticles were subsequently modified on the surface of polyaniline to immobilize a DNA aptamer. g-C 3 N 4 -heme incubated with different concentrations of A β solution to form g-C 3 N 4 -heme-A β complex, in which A β could specifically bind to the DNA aptamer and thus introduce g-C 3 N 4 into the system. As a result, it was observed that the ratio value of ECL intensity ( I 1 / I 2 ) significantly increased with the increase of A β concentration. Under the optimized conditions, the biosensor has a good linear response in the range 10 fM to 10 nM with a detection limit of 4.2 fM. Consequently, the ratiometric ECL sensor, characterized by high sensitivity and favorable stability, offers a reliable method for the determination of A β . Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tingyuan Pang,Shuting Ouyang,Jia Li,Xiaorui Liu,Wenying Shu,Wenhui Gao,Junren Li,Qi Song,Jianing Huang,Jun Yang,Xiaoshun Jian,Xiaole Wang,Meimei Zhang
Cervical cancer is one common cause of cancer relative death in women worldwide, while microRNA-21 (miR-21) can serve as one upregulated miRNA in cervical cancer for early diagnosis. Chemiluminescence is a powerful assaying method, but balancing the sensitivity and selectivity is often difficult at present. For this problem, a photocatalytic chemiluminescent biosensor was developed to homogeneously assay miR-21 with good performances. Based on a base stacking principle, cooperative hybridization is employed in double DNA probes for biosensor design. After DNA probe hybridization, photocatalytic DNA-dye complex can induce luminol’s chemiluminescence signals via several processes. Here the double-probe design results in the high sensitivity to assay miR-21 with a detection limit in pM level. Meanwhile, the short probe design and cooperative principle guarantee a good selectivity to discriminate other nucleotide variants. For comparison, both a single short and long probes were also studied to show the advantages of our biosensor in assay performances. So this photocatalytic chemiluminescent biosensor via cooperative hybridization is an ideal method to assay miR-21, showing a good potential for cancer early diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Liuyi Huang,Xinhui Gu,Fengna Xi
Establishing sensitive detection method for carbohydrate antigen 15–3 (CA15–3) is of great significance for the early diagnosis and therapeutic monitoring of breast cancer. Herein, an electrochemiluminescence (ECL) sensor was fabricated by growing Co 3 O 4 nanoparticles in-situ within nanochannels and employing a signal gating strategy, achieving highly sensitive detection of CA15–3. Using simple Stöber method, a silica nanochannel film (SNF) was deposited onto an indium tin oxide (ITO) glass electrode. This two-dimensional (2D) nanoporous silica film provided a confined space, within which Co 3 O 4 nanozymes were synthesized in situ. Co 3 O 4 served as a peroxidase (POD)-mimic nanozyme, enabling the catalytic conversion of hydrogen peroxide (H 2 O 2 ) into reactive oxygen species (ROS). Functioning as a nanocatalyst, Co 3 O 4 remarkedly enhanced the electrochemical oxidation of luminol, thereby boosting the ECL signal. This dual enhancement mechanism enabled the sensor to achieve high luminol-based ECL signal. The bio-recognition interface was constructed by functionalizing the outer surface of SNF with silane coupling agents and immobilizing aptamers. A measurable decrease in the ECL signal was generated due to the hindered diffusion of the emitter and co-reactant and increased interfacial resistance, which occurred when the aptamer-CA15–3 complex formed on the electrode surface. This response allowed for the quantification of CA15–3. Under optimal conditions, the developed aptasensor can detect CA15–3 in the range of 0.1 to 10,000 mU/mL, with a limit of detection of 26 μU/mL. Owing to its merits of simple fabrication, rapid analysis, high sensitivity, good reproducibility, and excellent selectivity, the developed immunosensor holds great potential for clinical diagnostics of tumor biomarkers.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 8.2
Haidong Li,Zhenxing Yang,Nidong Yangan,Xianjing Huang,Danli Zhong,Zhenzhen Wang,Chengyin Wang
The efficient activation of molecular oxygen to generate reactive oxygen species (ROS) offers great promise for achieving potential-resolved electrochemiluminescence (ECL) in the luminol/O2 system, though it typically relies on sophisticated catalysts. In this work, we demonstrate that carbonized polydopamine nanospheres (C-PDANSs) serve as an effective metal-free electrocatalyst to enhance the oxygen reduction reaction (ORR), thereby enabling the potential-resolved ECL of luminol. Mechanistic studies indicate that the ORR catalyzed by C-PDANSs proceeds via a two-electron pathway, yielding both superoxide radicals (O2–•) and hydrogen peroxide (H2O2). This ORR behavior facilitates the generation of sufficient ROS, which significantly boosts both cathodic and anodic luminol ECL, leading to 11.6-fold and 5.1-fold intensity enhancements, respectively. Based on the dual-potential ECL response, a ratiometric sensing platform was constructed for the detection of alkaline phosphatase (ALP). This study not only presents a different electrocatalyst for regulating dual-potential luminol ECL but also opens the avenue for designing biosensing schemes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）