Horticultural Plant Journal
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2017
·Vol. 3
·期 4
·165-171
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IF 0
Yanhui ZHANG,Peiying LIU,Qianqian KONG,Shiyan XING,Xiaojing LIU,Limin SUN
Flavonoids and terpene trilactones, especially, ginkgo flavonglycosides, ginkgolides and bilobalides in leaves of ginkgo trees, need to be studied for effective application of these active components with high medical and health-care values. This study was aimed to provide scientific bases for genealogies selection and harvest season confirmation for Ginkgo biloba . A high-performance liquid chromatographic method (HPLC-ELSD) was developed to determine the contents of terpene trilactone and flavonoid of 36 ancient G. biloba genealogies from 19 provinces in China. The study indicated that the content gradually increased from April to August, and thereafter declined. Analysis of variance indicated that the contents of terpene trilactone, flavonoids, and their respective components had significant difference among 36 genealogies. The cluster analysis showed that No. 72 (Xing'an, Guangxi), No. 58 (Youyang, Chongqing), No. 82 (Rugao, Jiangsu), No. 123 (Huixian, Gansu), No. 99 (Dujun, Guizhou), No. 10 (Tai'an, Shandong) and No. 133 (Mentougou, Beijing) genealogies have higher content of terpene trilactone and flavonoid. These results can help us to select superior variety containing high content of terpene trilactone and flavonoid.
研究领域:
植物化学
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药用植物学
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天然产物化学
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林木遗传育种
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药物代谢
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DOI
TheScientificWorldJOURNAL
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2014
·Vol. 2014
·期 1
·857982
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IF 1.219
Hongyu Xu,Gege Hu,Juane Dong,Qin Wei,Hongbo Shao,Ming Lei
In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. (30.07 mg/g·DW) was the highest, followed by those in C. fargesii Bur. (25.55 mg/g·DW) and C. ovata G. Don (24.96 mg/g·DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n -butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin ( 1 ) and apigenin ( 2 ), respectively.
研究领域:
植物化学
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天然产物化学
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药用植物学
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抗氧化研究
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分子生物学
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INDUSTRIAL CROPS AND PRODUCTS
·
2015
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IF 2.837
Xiao-Lin Qi,Xiao Peng,Yu-Yan Huang,Lu Li,Zuo-Fu Wei,Yuan-Gang Zu,Yu-Jie Fu
In the present study, deep eutectic solvents (DESs) as new extraction solvents, are proposed for the green and efficient extraction and separation of bioactive flavonoids from Equisetum palustre L. with negative pressure cavitation-assisted extraction (NPCE) combined with macroporous resin enrichment. A series of different DES systems were investigated for the extraction of bioactive flavonoids in E. palustre. Compared with conventional extraction solvents, DES composed of choline chloride/betaine hydrochloride-ethylene glycol possessed a more effective extraction yields. In addition, the DES-NPCE extraction procedures were also optimized systematically. The optimal conditions were obtained as follows: choline chloride-betaine hydrochloride-ethylene glycol formed a DES with the mole ratio of 1:1:2, 20% water content, extraction pressure −0.07 MPa, extraction temperature 60°C, solvent to solid ratio 25:1 mL/g, and the extraction time 20 min. Furthermore, the direct enrichment of nine flavonoids in DES extraction solution were efficiently achieved using macroporous resin HPD-826. The contents of the nine target flavonoids separated by HPD-826 resin were from 0.17% to 3.37%, and the recovery yields from 57.14% to 89.25%. In conclusion, the developed DES-NPCE combined with macroporous resin enrichment can be an alternative procedure for the green and efficient extraction and separation of bioactive flavonoids from plant sample matrices.
研究领域:
天然产物化学
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植物化学
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分析化学
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药物分离与纯化
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绿色化学
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Lirong Lu,Jiatian Zhu,Jingpeng Zhou,Xi Cheng,Baojie Liu,Chengrong Qin,Chen Liang,Caoxing Huang,Shuangquan Yao
Facing the urgent need for sustainable and functional food packaging, developing high-performance materials from natural polymers is crucial. Herein, we propose a green strategy inspired by crustacean exoskeletons to construct lignin–metal polyphenol networks (L-MPNs) via proton-based ionic liquid pretreatment and metal-ion coordination. This forms a stable 3D network utilizing lignin's phenolic hydroxyl groups, which significantly enhances antioxidant activity, achieves >99% UV shielding, and exhibits 92.5% DPPH radical scavenging capacity. Applied to paper packaging, Ferric-ion-coordinated lignin-metal polyphenol networks (FL-MPNs, 100 g/m 2 ) impart excellent barrier and antioxidant properties alongside maintained mechanical strength, cutting water vapor transmission by ∼40%. In freshness tests, they reduced mass loss to 3.52% and pH change to 0.17, effectively inhibiting food deterioration. Thus, FL-MPNs advance lignin valorization for next-generation sustainable smart packaging to ensure safer, more stable food preservation.
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.2
Hainan Song,Weiwei Cao,Xiushi Chen,Siqi Peng,Wenlong Sun,Xiaojun Yang,Mahmoud Salah,Mohamed A. Farag,Yajing Fang
Effects of four flavonoid inhibitors (FIs, quercetin, kaempferol, luteolin, and flavone) at different concentrations on dynamically increasing dihydroquercetin (DHQ) uptakes and regulating P-glycoprotein (P-gp)’s mRNA and protein levels were evaluated in KB/MDR1 cells. DHQ’s bidirectional transport was measured in Caco-2 monolayers. The low uptakes of DHQ were dynamically increased by four FIs (better than elacridar) in the order of quercetin > luteolin > kaempferol > flavone. Four FIs significantly increased DHQ40’s uptakes in a dose-independent manner. Four FIs alone or with DHQ significantly decreased P-gp’s mRNA and protein expressions. DHQ was poorly absorbed (efflux ratios >20) in Caco-2 monolayers, especially at high concentrations. Four FIs improved DHQ’s absorption by significantly increasing (reversing) its influx (efflux) at most time points (1, 2, 4, and 6 h), especially for DHQ100 rather than DHQ40. Docking results explained that quercetin competitively occupies DHQ’s binding site or binds to the inhibitor site of P-gp. These results were valuable for improving DHQ absorption.
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PubMed
高影响因子
Toxins
·
2023
·Vol. 15
·期 6
·365
·
IF 4.2
Zhongyuan Deng,Yuting Zhang,Liying Fang,Min Zhang,Lixiang Wang,Xinzhi Ni,Xianchun Li
Flavone is widely found in plants and plays an important role in plant defense against pests. Many pests, such asHelicoverpa armigera, use flavone as a cue to upregulate counter-defense genes for detoxification of flavone. Yet the spectrum of the flavone-inducible genes and their linkedcis-regulatory elements remains unclear. In this study, 48 differentially expressed genes (DEGs) were found by RNA-seq. These DEGs were mainly concentrated in the retinol metabolism and drug metabolism-cytochrome P450 pathways. Further in silico analysis of the promoter regions of 24 upregulated genes predicted two motifs through MEME and five previously characterizedcis-elements including CRE, TRE, EcRE, XRE-AhR and ARE. Functional analysis of the two predicted motifs and two different versions of ARE (named ARE1 and ARE2) in the promoter region of the flavone-inducible carboxylesterase geneCCE001jverified that the two motifs and ARE2 are not responsible for flavone induction ofH. armigeracounter-defense genes, whereas ARE1 is a new xenobiotic response element to flavone (XRE-Fla) and plays a decisive role in flavone induction ofCCE001j. This study is of great significance for further understanding the antagonistic interaction between plants and herbivorous insects.Keywords:Helicoverpa armigera;flavone;detoxification metabolism;carboxylesterase;cis-transcriptional regulation;AREKey Contribution:The spectrum of flavone up- and downregulated genes was revealed by transcriptomic analysis of the midgut of cotton bollworm treated with flavone, and an ARE-likecis-acting element in the promoter ofCCE001jwas found to determine whether the gene was induced by flavone.
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PubMed
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 4
·期 6
·1645-1655
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IF 12.958
Wang Chunyan,Hou Yaqi,Wang Xingyi,Li Weijun,Zhang Yunmao,Wang Shuli,Zheng Jing,Hou Xu
Catheterization is indispensable in the field of modern medicine. However, catheter-related thrombosis and infections almost inevitably occur during the process, and as drugs can only be administered at the end of catheter, auxiliary strategies are required for successful implantation. Considering these intractable limitations, a type of self-adaptive, anti-coagulate liquid-based fibrous catheter has been developed. More importantly, it has positional drug release property that traditional catheters desperately need but couldn’t attain. Although enlightening, the feasibility and performance of the positional drug release have only been demonstrated by fluorescents, the specific drug release kinetics remains unknown for adaptation to application scenarios. Therefore, we systematically investigate the structural and interfacial effects of drug molecules and fibrous matrixes on drug release kinetics in a liquid-based fibrous catheter. Theoretical calculations and experiments demonstrate that oleophilic and hydrophilic molecules release slowly due to a dissolution-diffusion mechanism. Amphipathic molecules, however, will significantly affect the gating performance by affecting the interfacial stability, hence they release quickly with emulsifying the gating liquid. Besides the significant impact of molecular properties and interfacial effects, matrix pore size also has a slight influence that molecules release faster in bigger pores. Through this study, the liquid-based fibrous catheter may step further toward practical applications including chemotherapy, haemodialysis, angiography, etc . to overcome the existing catheter-related limitations. Graphical Abstract
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Foods
·
2021
·Vol. 10
·期 11
·2709
·
IF 4.35
Yan Huang,Dandan Pu,Zhilin Hao,Xiao Yang,Yuyu Zhang
This work aims to explore the contribution of prickly ash (Zanthoxylum bungeanumMaxim) on the taste perception of stewed sheep tail fat. Liquid chromatography-tandem quadrupole time of flight mass spectrometry (LC-QTOF-MS) was applied to analyze the taste-related compounds. A total of 99 compounds in different sheep tail fat samples were identified. The semi-quantitative results showed that there were differences between the samples. The partial least squares discriminant analysis (PLS-DA) model without overfitting was used to investigate the effect of prickly ash. Eleven marker compounds were predicted with a variable importance for projection > 1, fold change > 2 andp< 0.05. An additional experiment showed that guanosine 5′-monophosphate, malic acid, inosine and adenosine 5′-monophosphate could improve the umami and saltiness taste of stewed sheep tail fat.Keywords:sheep tail fat;taste compounds;LC-QTOF-MS;PLS-DA;sensory analysis;additional experiment
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PubMed
高影响因子
Insect Science
·
2020
·Vol. 28
·期 6
·1756-1765
·
IF 2.791
Shan Wang,Min Zhang,Jinyong Huang,Leyao Li,Kaiyuan Huang,Yuting Zhang,Yalu Li,Zhongyuan Deng,Xinzhi Ni,Xianchun Li
Genetically engineered crops simultaneously produce defensive allelochemicals and Bacillus thuringiensis (Bt) toxin proteins to kill some of the world's most devastating insect pests. How the two types of toxins, when ingested sequentially or simultaneously, interact at both lethal and sublethal doses in these pests remains underexplored. Here, we examined the toxicological interactions between the Bt toxin Cry1Ac and the flavonoid allelochemical flavone in Helicoverpa armigera . Simultaneous exposure of H. armigera neonates to lethal doses (LC 25 ) of Cry1Ac and flavone caused a mortality significantly higher than that of either toxin alone and their expected additive mortality. Preexposure for 24 h to a sublethal dose (LC 10 ) of Cry1Ac followed by 6-d simultaneous exposure to the same dose of Cry1Ac plus a lethal dose (1.6 mg/g diets, LC 50 ) of flavone resulted in a mortality significantly higher than that of the LC 50 dose of flavone alone and the expected additive mortality of the LC 50 dose of flavone plus the LC 10 dose of Cry1Ac. One-day preexposure to the sublethal dose (LC 10 ) of flavone followed by 6-d simultaneous exposure to the LC 50 dose (6 ng/cm 2 ) of Cry1Ac plus the LC 10 dose of flavone yielded a mortality significantly higher than that of the LC 50 dose of Cry1Ac but similar to the expected additive mortality of the LC 50 dose of Cry1Ac plus the LC 10 dose of flavone. The results suggest that Cry1Ac induces and synergizes the toxicity of flavone against H. armigera larvae.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xinran Wang,Xufeng Zang,Hong Zhou,Na Wang,Yishan Fang,Bo Cui
Trichlorfon (TCF) has the possibility of contaminating agricultural crops and posing some health risks to humans. Herein, an electrochemiluminescence (ECL) sensor based on Fe, Cu dual-atom catalysts (Fe/Cu-N-C DACs) and Au@Luminol was developed for the ultrasensitive detection of TCF. Fe/Cu-N-C with diatomic sites has a very high catalytic activity and can be used as a co-reaction accelerator to activate H 2 O 2 to generate a large number of hydroxyl radicals which triggered a strong cathodic ECL signal of luminol. TCF molecularly imprinted polymer (MIP) was further introduced as a specific recognition element, and the interaction between the template molecule and the functional monomer was verified by molecular docking technique. The developed sensing platform was successfully applied to the ultrasensitive detection of TCF with a linear range from 1.0 pg/mL to 5.0 μg/mL with a low detection limit (0.39 pg/mL). This study broadens the application of DACs in ECL sensing.
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PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 13
·3132
·
IF 4.2
Pengjie Zhang,Bin Du,Jiwei Xu,Jiang Wang,Zhiwei Liu,Bing Liu,Fanhua Meng,Zhaoyang Tong
Sensitively detecting hazardous and suspected bioaerosols is crucial for safeguarding public health. The potential impact of pollen on identifying bacterial species through fluorescence spectra should not be overlooked. Before the analysis, the spectrum underwent preprocessing steps, including normalization, multivariate scattering correction, and Savitzky–Golay smoothing. Additionally, the spectrum was transformed using difference, standard normal variable, and fast Fourier transform techniques. A random forest algorithm was employed for the classification and identification of 31 different types of samples. The fast Fourier transform improved the classification accuracy of the sample excitation–emission matrix fluorescence spectrum data by 9.2%, resulting in an accuracy of 89.24%. The harmful substances, includingStaphylococcus aureus, ricin, beta-bungarotoxin, and Staphylococcal enterotoxin B, were clearly distinguished. The spectral data transformation and classification algorithm effectively eliminated the interference of pollen on other components. Furthermore, a classification and recognition model based on spectral feature transformation was established, demonstrating excellent application potential in detecting hazardous substances and protecting public health. This study provided a solid foundation for the application of rapid detection methods for harmful bioaerosols.
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PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Li-Feng Li,Meng-Di Wang,Chen-Yang Zhang,Meng-Yao Jin,Hua-Lei Chen,Huan Luo,Tian-Yu Hou,Zhi-Jun Zhang,He Li
Advanced glycation end products (AGEs) generated from the Maillard reaction (MR) during food processing in the human physiological environment, have been proven to be significantly associated with various chronic metabolic diseases. In this study, 18 flavonoids were investigated to study their effects on AGEs formation during MR within a glucose-lysine-arginine model system. Five AGEs— N ε -carboxymethyl-lysine (CML), N ε -carboxyethyl-lysine (CEL), pyrraline, pentosidine, and argpyrimidine—were determined by high-performance liquid chromatography-mass spectrometry, with inhibitory rates ranging between 0 % and 71.35 %. Isorhamnetin and naringenin exhibited the strongest inhibitory effect on the formation of CML and CEL, respectively, whereas myricetin exhibited the strongest inhibitory effect on pyrraline, pentosidine, and argpyrimidine formation. In addition, each flavonoid was reacted with glyoxal (GO) and methylglyoxal (MGO) to investigate their trapping activities and adducts. The results showed that the 18 flavonoids could effectively clear GO and MGO, with clearance rates of 1.03 %–71.42 % and 0.93 %–69.37 % for GO and MGO, respectively. Six flavonoids—chrysin, naringenin, apigenin, luteolin, diosmetin, and kaempferol—could form adduct products with mono-/di-MGO, with flavonoid to mono-/di-MGO adduct ratios of approximately 6:1, 2:1, 8:1, 23:1, 10:1, and 3:1, respectively. The number and site of phenolic hydroxyl groups, as well as methoxy substitution on the B-ring in flavonoids, had little effect, but phenolic hydroxyl groups at the C-3 position in the C-ring impeded adduct formation. The flavonoids lowered the degradation of glucose itself, and decreased ammonia-induced degradation and Amadori rearrangement product oxidation due to their antioxidant activities, and they trapped the reactive 1,2-di‑carbonyl species via nucleophilic addition reaction to form AGEs.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）