Separations
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2026
·Vol. 13
·期 8
·232
·
IF 3.5
Chuyue Zhang,Le Duan,Xu Wang,Lanlan Qiu,Deli Xiao
Reversed-phase chromatography stationary phases (such as C18) are widely used in commercial high-performance liquid chromatography (HPLC). However, when dealing with complex mixtures, they often exhibit limited separation capabilities and peak tailing phenomena. To address this problem, two novel HPLC stationary phases based on polyamine macrocycles were developed. A trianglamine macrocyclic stationary phase (TRI-Sil) was first prepared using chlorinated silica gel as the support, which can effectively separate a variety of aromatic compounds, but with limited selectivity for positional isomers such as phenylenediamine. To further improve the separation selectivity, a polyamine-silica macrocyclic stationary phase (CPAM-Sil) was synthesized by introducing a branched-chain-containing monomer. Under optimized conditions, CPAM-Sil achieved baseline separation of phenylenediamine and phenylenediol positional isomers and improved the separation of terphenyl isomers, with favorable asymmetry factors and high column efficiency compared with the commercial C18 column. Molecular docking confirmed multiple interactions such as electrostatic interactions and hydrogen bonding between the polyamine macrocycle (CPAM) and analytes. The CPAM-Sil column also exhibited good reproducibility and stability, showing promising potential for industrial application in chromatographic separation.
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Microorganisms
·
2026
·Vol. 14
·期 1
·158
·
IF 4.2
Shuyan Jiang,Jianhong Sun,Ning Jin,Shuya Wang,Shuchao Huang,Zhaozhuang Li,Jihua Yu,Jian Lyu,Li Jin
In facility tomato production, the excessive application ratio of ammonium nitrogen (NH 4 + -N) often leads to root acidification and calcium-magnesium antagonism. Although amide nitrogen (urea-N) has better buffering properties, it needs to be hydrolyzed before utilization, resulting in a lag effect. Previous studies have mostly focused on a single nitrogen source or a fixed proportion, and there is still a lack of systematic evidence on the nitrogen supply effects of different nitrogen application combinations at different growth stages of tomatoes. Therefore, in this experiment, tomato cultivar ‘Jingfan 502’ was used. All treatments received the same total nitrogen concentration (15 mM), but the nitrogen was supplied as different combinations of ammonium nitrogen (AN) and amide nitrogen (UN). Six AN–UN ratio treatments were designed: CK (0% AN, 0% UN), T1 (100% AN, 0% UN), T2 (0% AN, 100% UN), T3 (25% AN, 75% UN), T4 (50% AN, 50% UN), and T5 (75% AN, 25% UN). T3 (25% NH 4 + + 75% urea) increased single-plant yield by 64.04% and 5.10%, and total N, P, K, and Mg accumulation by 29.0% and 20.7%, relative to T1 and T2. In addition, compared to T1 and T2, the nitrogen fertilizer uptake rate of the T3 treatment increased by 17.00% and 24.90%, respectively, and the electrical conductivity (EC) increased by 27.04% and 44.84%, respectively. Redundancy Analysis (RDA) showed that enzyme activities, total N and electrical conductivity were positively linked to microbial communities in T3 and T4, whereas communities in CK, T1, T2 and T5 correlated with nutrients and pH. Under controlled pot conditions, T3 optimizes the rhizosphere micro-environment, enhances microbial abundance and nutrient uptake, and provides a theoretical basis for precise N management in tomato.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Donglin Xiang,Xiao Wang,Chun Ji,Laping He,Yuangen Wu,Han Tao
This study presents a novel electrochemical strategy for detecting organophosphorus pesticides based on the synergistic catalytic of red kidney bean esterase (RKBE) and iron–cobalt dual-atom catalyst embedded in N-doped carbon carrier (FeCo–NC). RKBE was extracted from the widely cultivated British red kidney beans ( Phaseolus vulgaris L.) . FeCo–NC exhibited excellent electrocatalytic and peroxidase-like activity, catalyzing o -phenylenediamine oxidation to produce electroactive 2,3-diaminophenazine, which served as signal probe generating a sensitive cathodic current. Notably, RKBE product 1-naphthol inhibited FeCo–NC activity, while organophosphorus pesticides counteracted this inhibition by suppressing RKBE activity, restoring the electrochemical signal of 2,3-diaminophenazine. Leveraging this signal-regulation mechanism, a dichlorvos sensor was developed, with wide linear range (50–50,000 pM), low detection limit (14 pM), and excellent real-sample recovery (93.33%–107.08%), demonstrating practical application potential. This study provides an affordable and easy-to-operate approach for large-scale on-site monitoring of organophosphorus pesticide residues in foods.
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Hengye Li,Xingwei Han,Lin Zhang,Wenjie Yu,Wenwen Bie,Meijie Wei,Zhongxia Wang,Fenying Kong,Wei Wang
Herein, β-cyclodextrin-containing hybrid polymers (P1, P2 and P3) were prepared through crosslinking partially benzylated β-cyclodextrin (PBCD) by octavinylsilsesquioxane (OVS). P1 stood out in screening studies and the residual hydroxyl groups of PBCD was sulfonate-functionalized. The obtained P1-SO 3 Na showed greatly enhanced adsorption towards cationic MPs and maintained the excellent adsorption performance towards neutral MPs. The rate constants ( k 2 ) of cationic MPs upon P1-SO 3 Na were 9.8–34.8 times larger than those upon P1. The equilibrium uptakes of the neutral and cationic MPs upon P1-SO 3 Na were above 94.5 %. Meanwhile, P1-SO 3 Na demonstrated appreciable adsorption capacities, excellent selectivity, effective adsorption of mixed MPs at environmental levels and good reusability. These results confirmed the great potential of P1-SO 3 Na as effective adsorbent to remove MPs from water.
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CRYSTENGCOMM
·
2022
·Vol. 25
·期 3
·432-443
·
IF 3.756
Yuping Li,Yang Tao,Yunping Zhoujin,Fang Zhao,Peng-Yu Liang,Sean Parkin,Tonglei Li,Pan-Pan Zhou,Sihui Long
Substituent size and isomerization play an important role in the polymorphism of 2-(naphthalen-n-ylamino)-benzoic acids (n = 1, 2; 2-NBAs) as each of the two previously investigated 2-NBAs was found to be polymorphic, while their parent compound fenamic acid (FA) has only one form discovered. 2-(Naphthalen-1-ylamino)-nicotinic acid (1) and 2-(naphthalen-2-ylamino)-nicotinic acid (2) (2-NNA) are isosteric compounds of 2-NBAs, and 2-(2,3-dimethyl-phenylamino)-nicotinic acid (3) and 2-(3,4-dimethyl-phenylamino)-nicotinic acid (4) are 2-NNA analogues with similar substitution patterns. All four compounds are clonixin analogues. The polymorphism of these four compounds was investigated. One solvent-free form (1-A) and one hydrate (1-H) were discovered for 1, three forms (2-I, 2-II, and 2-III) were obtained for 2, and one form was found for 3 and 4. The four systems were characterized by single-crystal XRD (SCXRD) and powder XRD (PXRD), and structural properties were compared among the crystals of the four compounds, their isosteres, and other related analogues. The phase behavior of the four systems was studied with differential scanning calorimetry (DSC). Thermogravimetric analysis (TGA) was used to investigate the dehydration of 1-H. Computational studies such as stable conformer search, π–π interaction energy calculation, and non-covalent interaction (NCI) analysis were performed to shed light on the rise of polymorphism, and the relative contribution of various intermolecular interactions to the overall stability of the crystals. The study confirmed the effect of substituent size, thus the consequent π–π interactions, on the hydrate formation of compound 1 and polymorphism of compound 2.
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MOLECULES
·
2022
·Vol. 27
·期 20
·6952
·
IF 4.927
Liqiong Sun,Zhijuan Tang,Minxin Wang,Jun Shi,Yajuan Lin,Tiefeng Sun,Zhilu Zou,Zebin Weng
With the abuse of antibiotics, bacterial antibiotic resistance is becoming a major public healthcare issue. Natural plants, especially traditional Chinese herbal medicines, which have antibacterial activity, are important sources for discovering potential bacteriostatic agents. This study aimed to develop a fast and reliable method for screening out antimicrobial compounds targeting the MRSA membrane fromPsoralea corylifoliaLinn. seed. A UPLC-MS/MS method was applied to identify the prenylated flavonoids in major fractions from the extracts ofPsoralea corylifoliaLinn. seed. The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of different fractions and compounds. The morphological and ultrastructural changes of MRSA were determined by scanning electron microscopy (SEM). The membrane-targeting mechanism of the active ingredients was explored by membrane integrity assays, membrane fluidity assays, membrane potential assays, ATP, and ROS determination. We identified eight prenylated flavonoids inPsoralea corylifoliaLinn. seed. The antibacterial activity and mechanism studies showed that this type of compound has a unique destructive effect on MRSA cell membranes and does not result in drug resistance. The results revealed that prenylated flavonoids inPsoralea corylifoliaLinn. seeds are promising candidates for the development of novel antibiotic agents to combat MRSA-associated infections.Keywords:methicillin-resistantStaphylococcus aureus;Psoralea corylifoliaLinn. seed;prenylated flavonoids;UPLC-MS/MS;bacterial membrane
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Qidong Yu,Wenmin Zhang,Hui Chen,Jingyi Wang,Zhiyong Wang,Qingqing Ding,Lan Zhang
Background Developing facile and general functionalization strategies to improve the durability of covalent organic framework (COF) coatings and their affinity for polar targets is of great significance for solid-phase microextraction (SPME) technology . Results In this work, a facile and general amidation strategy was developed for conversion from reversible (imine) to irreversible (amide) linkages in COF coatings. After the amidation, the durability of the obtained amide-linked covalent organic framework (Am-P-COF) coating was greatly improved, and the adsorption efficiency for polar aromatic amines (AAs) was also significantly increased. Moreover, this strategy is also applicable to the amidation of other two COF coatings, showing good general applicability. The obtained Am-P-COF coated fiber was used for SPME, and then coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) to detect AAs. Under the optimal SPME conditions (extraction temperature: 50 °C, extraction time: 30 min, stirring rate: 600 rpm, pH: 8, NaCl concentration: 5.0 mg mL −1 , desorption temperature: 290 °C and desorption time: 10 min), a detection method for trace AAs was established. The established method possess wide linear ranges (0.5–500.0 ng L −1 ), good correlation coefficients (0.9986–0.9993) and low detection limits (0.1–0.5 ng L −1 ). Moreover, the established method had also been successfully applied to detection of trace AAs in bottled tea beverage and plastic bags packed tea with satisfactory recoveries (83.5 %–116.8 %). Significance and novelty This research provides a facile and general pathway for increasing the durability of COF coatings and affinity to the polar AAs. The detection method based on the obtained fibers possesses high sensitivity, satisfactory reproducibility and good precision.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Liangliang Gao,Yan Liang,Qin Xiong,Meiling Huang,Yueming Jiang,Jun Zhang
Citrus blue and green molds caused by Penicillium digitatum , P. italicum , and P. polonicum , are the major postharvest diseases of citrus fruit. In the present study, Actinomycin X 2 (Act-X 2 ), a naturally occurring antibiotic produced by Streptomyces species, was found to show excellent antifungal effect against these three pathogens with a minimum inhibitory concentration (MIC) value of 62.5 μg/mL for them all, which was better than the positive control thiophanate-methyl. Act-X 2 significantly reduced the percentage of spore germination , and highly inhibited the mycelial growth of P. italicum , P. digitatum , and P. polonicum with EC 50 values being 34.34, 13.76, and 37.48 μg/mL, respectively. In addition, Act-X 2 greatly decreased the intracellular protein content while increasing the reactive oxygen species (ROS) level and superoxide anion (O 2 − ) content in the mycelia of pathogens. In vivo test indicated that Act-X 2 strongly inhibited the infection of navel oranges by these three Penicillium species, with an inhibition percentage of >50% for them all at the concentration of 10 MIC. Transcriptome analysis suggested that Act-X 2 might highly influence the ribosomal functions of P. polonicum , which was supported as well by the molecular docking analysis of Act-X 2 with some key functional proteins and RNAs of the ribosome. Furthermore, Act-X 2 significantly reduced the decay percentage and improved the firmness, color, and sugar-acid ratio of navel oranges spray-inoculated with P. polonicum during the postharvest storage at 4 °C for 60 d.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Qingqing Li,Wenmin Zhang,Yuheng Guo,Hui Chen,Qingqing Ding,Lan Zhang
In this study, an oxygenated carbon nanotubes cages (OCNTCs) material was prepared by calcinating zeolitic imidazole framework-67 (ZIF-67) and then oxidizing the resulting material. The OCNTCs was used as a high efficient solid-phase microextraction (SPME) coating to extract aromatic amines (AAs). The obtained fiber exhibited high selectivity for AAs over other organic compounds in food contact materials (FCMs) due to matched pore size and abundant oxygen-containing groups. Subsequently, coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS), a sensitive method with low limits of detection (0.1-2.0 ng L −1 ), wide linear ranges (0.5-500 ng L −1 ) and good precision (RSDs ≤ 8.6%) was developed for analysis of AAs. The specific migrated AAs from food simulants that prepared by standardized migration and thermal migration test were successfully analysed by this developed method with satisfactory recoveries (81.6% - 118.1%) and precision (RSDs, 2.1-9.5%). The results demonstrated that the prepared OCNTCs-coated fibers displayed excellent extraction performance, suggesting a promising application to investigate the migration behaviors of AAs.
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Molecular Catalysis
·
2020
·
IF 3.687
Huan Fu,Luna Ruan,Jianhua Liao,An Pei,Jun Liu,Li Zeng,Kai Yang,Lihua Zhu,Bing Hui Chen
In this report, the Ni/C sample was successfully prepared at room temperature (RT) by depositing nickel related species on carbon black by a chemical liquid reduction method with hydrazine hydrate as reducing agent, Pt/C was synthesized via a wetness impregnation method. And Pt was supported on Ni/C by galvanic replacement reaction to form PtNi/C bimetallic nanocatalyst. By comparing the catalytic performance of different catalysts for 1-nitronaphthalene hydrogenation, it could be found that the PtNi/C bimetallic nanocatalyst showed higher catalytic activity than monometallic nanocatalysts (Pt/C and Ni/C), exhibiting 100 % conversion of 1-nitronaphthalene, 100 % selectivity to 1-naphthylamine and turnover frequency of 194.9 h −1 under mild reaction conditions (40 min, 30 °C, 3.0 MPa H 2 ). The as-prepared catalysts were characterized by XRD, XPS, HAADF-STEM, STEM-EDX mapping and line-scanning, TG, HRTEM and H 2 -TPD, which proved that PtNi/C showed most outstanding catalytic behaviors in 1-nitronaphthalene selective hydrogenation due to high dispersion of platinum, the nanostructure of Pt-on-Ni-on-Ni(OH) 2 and thus synergism effect among Pt, Ni and Ni(OH) 2 .
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ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Zaiming Chen,Jianfei Wang,Yuefeng Wang,Bing Li,Meizhen Wang
Pyrogenic char (biochar) with a high sorption capacity (B-HSC) can sequester hazardous chemicals (e.g., phenanthrene). However, when sorption inhibits bioavailability of some functional chemicals (e.g., the herbicidal efficacy of diuron in soil), biochar with a low sorption capacity (B-LSC) is required to prevent sorption effects. The pyrolytic B-HSC generation has been reported, but information on B-LSC formation is scarce. How fast B-HSC and B-LSC could be generated is unknown until now. Here, biochars were rapidly prepared (the shortest heating time reached 5 min and the cooling time reached < 30 min) by a direct-pyrolysis method by directly exposing packaged rice straw and pine wood to 350 °C, 500 °C and 700 °C and out-of-furnace cooling at room temperature. The sorption of diuron, phenanthrene , and twelve other chemicals was investigated. B–HSCs were obtained within 30 min of rice straw pyrolysis , and the biochar K d values quickly increased to 7-730-fold that of the raw biomass as –OH and C–O–C in (hemi)cellulose of rice straw rapidly degraded, increasing hydrophobic interactions between the char and chemicals (solubility ≤ 82.8 g/L). In contrast, B-LSCs were generated within 30 min of PW pyrolysis, and the K d values of the biochars were 0.2–3.0-fold that of the raw biomass, as the surface area development and hydrophobicity-driven sorption were probably delayed by the late degradation of lignin aromatic C–O and phenolic –OH. Biochar amendment revealed an enhancement effect of B-HSC but not of B-LSC on soil sorption. The fast formation of B-LSC and B-HSC provides a guide to develop time- and cost-effective technique in pyrolytically producing weakly or strongly sorbing biochars for organic chemical management.
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TALANTA
·
2020
·
IF 5.339
Zong-Da Du,Yuan-Yuan Cui,Cheng-Xiong Yang
Microporous organic networks (MONs) are a potential class of microporous materials in analytical chemistry . As great efforts have been made in sample pretreatment and gas chromatography, the application of MONs in high performance liquid chromatography (HPLC) has largely lagged and has never been investigated. In addition, directly packing of MONs into columns for HPLC is impractical due to the high column pressure and low column efficiency resulted from the sub-micron sized and irregular MONs. To solve these issues and uncover the possibility of MONs in HPLC, here we report the fabrication of spherical silica amino-functionalized MON (SiO 2 @MON-NH 2 ) composite as a novel stationary phase for HPLC. Neutral, acidic and alkaline probes were evaluated on SiO 2 @MON-NH 2 packed column. A column efficiency of 21,246 plates per meter was obtained for n -propylbenzene. The results revealed the hydrophobic interaction was the main separation mechanism on SiO 2 @MON-NH 2 packed column. Baseline separation of benzenediol , dichlorophenol , chlorophenol and phenylenediamine position isomers, deoxynucleosides and nucleosides was also achieved on SiO 2 @MON-NH 2 packed column. The relative standard deviations of retention time, peak height and peak area for the studied position isomers, deoxynucleosides and nucleosides were 0.2–0.7%, 0.3–3.7% and 1.7–3.5%, respectively. The SiO 2 @MON-NH 2 packed column also offered better resolution for dichlorophenol isomers, deoxynucleosides and nucleosides than SiO 2 –NH 2 and commercial C18 columns. These results revealed the potential of MONs in HPLC and also provided a new way for the use of nano-sized or irregular MONs in HPLC.
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