BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2019
·Vol. 67
·期 3
·343-353
·
IF 1.559
Hai-Feng Hu,Hai-Yan Zhou,Gao-Ping Cheng,Ya-Ping Xue,Yuan-Shan Wang,Yu-Guo Zheng
R -2-(4-hydroxyphenoxy)propionicacid (HPOPA) is a valuable intermediate for the synthesis of enantiomerically pure aryloxyphenoxypropionic acid herbicides. In this work, to improve the HPOPA biosynthesis by Beauveria bassiana ZJB16002 from the substrate R -2-phenoxypropionic acid (POPA), the original HPOPA producer B. bassiana ZJB16002 was subjected to physical mutagenesis with 137 Cs-γ irradiation and chemical mutagen N -methyl- N' -nitro- N -nitrasoguanidine (NTG) induced mutagenesis. The effects of different treatment doses of the mutagens on the lethal rate and positive mutation rate were investigated, and the results showed that the optimal 137 Cs-γirradiation dose and NTG concentration was 850 Gy and 500 µg/mL, respectively. Under these conditions, a mutant strain CCN-7 with the highest HPOPA production capacity was obtained through two rounds of 137 Cs-γ irradiation treatment followed by one round of NTG mutagenesis. At the substrate (POPA) concentration of 50 g/L, HPOPA titer of CCN-7 reached 36.88 g/L, which was 9.73-fold higher than the parental strain. The morphology of the wild-type and mutant strain was compared and the results might provide helpful information in exploration of the correlation of morphology and biochemical features of B. bassiana .
研究领域:
微生物学
·
生物工程
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DOI
PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2015
·Vol. 30
·期 5
·1098-1102
·
IF 0.399
Zhou Yong,Liu Fude,Wu Haiyan,Duan Lijie,Qu Boyang
One conjugated polymer consisting of carbazole and 3-hexylthiophene moiety, poly (N-Hexyl-2,7-di(2-(4-hexylthiophene)carbazole) (PNDC), has been synthesized by ferric chloride oxidative polymerization. The monomer N-Hexyl-2,7-di(2-(4-hexylthiophene)carbazole was synthesized and characterized by IR, 1 H-NMR, 13 C-NMR and MS. UV-vis absorption spectrum, fluorescence spectrum, photoluminescence spectrum and electrochemical properties of the polymer were investigated. Polymer PNDC shows maximum peak appearing at 394 nm in UV-vis absorption spectrum, strongest fluorescence-emission at 482 nm in fluorescence spectrum and maximum emission peak at 492 nm in photoluminescence spectrum. The band-gap ( E
g ), HOMO energy ( E
HOMO ), and LUMO energy ( E
LUMO ) of the polymer were obtained as 2.58, -5.32, and -2.74 eV, respectively.
研究领域:
材料科学
·
有机化学
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DOI
高影响因子
JOURNAL OF MICROBIOLOGICAL METHODS
·
2019
·
IF 1.803
Hai-Feng Hu,Hai-Yan Zhou,Mei-Xin Wang,Yuan-Shan Wang,Ya-Ping Xue,Yu-Guo Zheng
( R )-2-(4-hydroxyphenoxy)propionic acid (( R )-HPOPA) is an important intermediate for the synthesis of optically pure aryloxyphenoxypropionic acid herbicides. Regioselective hydroxylation of ( R )-2-phenoxypropionic acid (( R )-POPA) by microbes is one of the most useful methods for the industrial production of ( R )-HPOPA. In this study, we designed and optimized a rapid throughput assay for screening ( R )-HPOPA producing bacterial/fungal strains which can regioselectively hydroxylate ( R )-POPA. ( R )-HPOPA could react with 4-aminoantipyrine (4-AAP) in the presence of potassium hexacyanoferrate (K 3 [Fe(CN) 6 ]) to form indoxyl antipyrine, an orange-red chromophore, that can easily spectrophotometrically be determined at 550 nm. During the verification of the assay we observed an average recovery rate of between 97.3% and 104.5%. Apart from the rapid throughput, no obvious differences in detection ( R )-HPOPA in the culture broth samples were found between our rapid throughput multiplate assay and a high-performance liquid chromatography method. Our optimized assay method is simple, rapid and accurate with high repeatability. It has the potential for high throughput screening (about 3000–5000 samples/day) of the ( R )-HPOPA producing strains.
研究领域:
生物化学
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微生物学
·
工业生物技术
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DOI
PubMed
高影响因子
LIQUID CRYSTALS
·
2013
·
IF 1.959
Lina Zhang,Xinbing Chen,Fengqi Zhao,Xuezhong Fan,Pei Chen,Zhongwei An
Three series of 2-(4′-alkoxybiphenyl-4-yl)-1H-benzimidazole derivatives (nM-x), which possessed 5-nitrobenzimidazole (nM-N series), benzimidazole (nM-H series) or 5-methylbenzimidazole (nM-M series) units at the end of the molecule, were synthesised and characterised by infrared, 1H- and 13C-nuclear magnetic resonance spectra, electrospray ionisation-mass spectrometry and elemental analysis. Their phase transition behaviour was investigated by differential scanning calorimetry, polarising optical microscopy and X-ray diffraction. All the compounds exhibited enantiotropic smectic mesophases with wide temperature domains for a carbon number in the alkoxy chain from 6 to 16, where the mesophase ranges were 14–91°C and 17–99°C during heating and cooling processes for the nM-N compounds, 7–25°C and 8–49°C for the nM-H compounds and 48–81°C and 52–85°C for the nM-M compounds, respectively. The effect of the length of alkoxy chain on mesomorphic properties was discussed. The nM-N and nM-M exhibited a much wider mesophase range whether during heating or cooling process than the corresponding nM-H series, especially for the longer terminal chain (n > 8), which indicated that the substituent in the benzimidazole moiety was helpful in increasing the mesophase stability.
研究领域:
有机化学
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材料科学
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DOI
高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2013
·
IF 0.596
Zhiping Du,Tianhua Shen,Haibo Dang,Zhenfang Fu,Qingbao Song
A simple efficient method for synthesis of Schiff bases in good yields from aromatic aldehydes and 2-(4-aminophenyl)acetonitrile catalysed by 3,5-difluoroarylboric acid at room temperature for 2 hours is reported.
研究领域:
有机化学
·
合成化学
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DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2019
·Vol. 42
·期 10
·1573-1582
·
IF 2.371
Zhou Hai-Yan,Li Yi-Zuo,Jiang Rui,Hu Hai-Feng,Wang Yuan-Shan,Liu Zhi-Qiang,Xue Ya-Ping,Zheng Yu-Guo
R -2-(4-hydroxyphenoxy)propionic acid ( R -HPPA) is a key intermediate of the enantiomerically pure phenoxypropionic acid herbicides. R -HPPA could be biosynthesized through selective introduction of a hydroxyl group (–OH) into the substrate R -2-phenoxypropionic acid ( R -PPA) at C-4 position, facilitated by microorganisms with hydroxylases. In this study, an efficient high-throughput screening method for improved R -HPPA biosynthesis through microbial hydroxylation was developed. As a hydroxylated aromatic product, R -HPPA could be oxidized by oxidant potassium dichromate to form brown-colored quinone-type compound. The concentration of R -HPPA can be quantified according to the absorbance of the colored compound at a suitable wavelength of 570 nm; and the R -HPPA biosynthetic capability of microorganism strains could also be rapidly evaluated. After optimization of the assay conditions, the high-throughput screening method was successfully used in identification of Beauveria bassiana mutants with enhanced R -HPPA biosynthesis capacity. A positive mutant C-7 with high tolerance to 20 g/L R -PPA was rapidly selected from 1920 mutants. The biomass and R -HPPA titer were 12.5- and 38.19-fold higher compared with the original strain at 20 g/L R -PPA. This high-throughput screening method developed in this work could also be a potential tool for screening strains producing other important phenolic compounds.
研究领域:
生物技术
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微生物学
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化学工程
·
药学
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DOI
PubMed
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2020
·
IF 4.032
Yuehan Qian,Fuhao Dong,Lizhen Guo,Jiawen Guo,Hiba Shaghaleh,Yuqi Wang,Xu Xu,Shifa Wang,Shiwei Liu
Limonene is an important subsidiary agricultural product obtained from orange peel that can also be prepared by microbial engineering, but its application degree of deep processing is relatively lower. By studying the application of limonene to silicone rubber, the application field of limonene has been expanded. Triethoxy (2-(4-methylcyclohex-3-en-1-yl)propyl)silane (LTES) was synthesized by a hydrosilylation reaction in the presence of Karstedt's catalyst using limonene and triethoxysilane as raw materials. FT-IR and 1 H NMR were used to determine the structure of the product (LTES). Thereafter, a series of room temperature vulcanized (RTV) silicone rubbers was modified with the LTES cross-linking agent and hydroxy silicone oil under the catalytic action of dibutyltin dilaurate. The results showed that the tensile strength , elongation at break and heat resistance of the synthetic silicone rubber modified with LTES were significantly higher.
研究领域:
材料科学
·
有机化学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Zheng-En Piao,Jingzhou Guo,Dan Wang,Wei Mao,Yuan Pu,Jian-Feng Chen
2-(4-tert-Butylphenyl)-5-(4-biphenyl)-1,3,4-oxadiazole (PBD) is one of the most widely used electron transport layer materials in optoelectronic devices, and studies on the solubility of PBD in various solvents are critical for development of solution-processable roll-to-roll electronic devices. In this study, the isothermal saturation method is used to determine the solubility data of PBD in 12 commonly used organic solvents. The experimental solubility of PBD increases gradually with enhanced temperature for the examined temperature range of 283.15–323.15 K in n-hexane, methanol, ethanol, isopropanol, tetrahydrofuran (THF), acetonitrile, ethyl acetate, N-methylpyrrolidone (NMP), toluene, and chlorobenzene, while the temperature ranges were 293.15–333.15 K and 273.15–303.15 K for dimethyl sulfoxide and dichloromethane, respectively. The obtained solubility values of PBD are correlated by three models including the modified Apelblat equation, λh equation, and Yaws equation. Comparison of the calculated Akaike information criterion values resulted in selecting the optimal model for each solvent. Of the five solvents calculated, n-hexane, methanol, toluene, THF, and NMP, the λh equation provides an optimal fit. Both the modified Apelblat equation and the Yaws equation work well in chlorobenzene. Among the other six solvents, the fit of the Yaws equation is the best. These results of solubility provide the basic data for the synthesis of PBD nanoparticles via high-gravity-assisted antisolvent precipitation processing.
研究领域:
材料科学
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化学工程
·
物理化学
·
光电子学
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DOI
高影响因子
WATER ENVIRONMENT RESEARCH
·
2024
·Vol. 96
·期 2
·e10984
·
IF 2.5
Xinchao Ruan,Huan Wang,Fengyun Huang,Fanye Wang,Xiaojun Yang
In this study, a highly efficient peroxymonosulfate (PMS) activator, ZnO/ZnMn 2 O 4 , was synthesized using a simple one-step hydrothermal method. The resulting bimetallic oxide catalyst demonstrated a homogenous and high-purity composition, showcasing synergistic catalytic activity in activating PMS for degrading 2, 4-dichlorophenol (2, 4-DCP) in aqueous solution. This catalytic performance surpassed that of individual ZnO, Mn 2 O 3 , and ZnMn 2 O 4 metal materials. Under the optimized conditions, the removal efficiency of 2, 4-DCP reached approximately 86% within 60 min, and the catalytic ability remained almost constant even after four cycles of recycling. The developed degradation system proved effective in degrading other azo-dye pollutants. Certain inorganic anions such as HPO 4 − , HCO 3 − , and NO 3 − significantly inhibited the degradation of 2, 4-DCP, while Cl − and SO 4 2− did not exhibit such interference. Results from electrochemical experiments indicated that the electron transfer ability of ZnO/ZnMn 2 O 4 surpassed that of individual metals, and electron transfer occurred between ZnO/ZnMn 2 O 4 and the oxidant. The primary active radicals responsible for degrading 2, 4-DCP were identified as SO 4 •− , OH • and O 2 •− , generated through the oxidation and reduction of PMS catalyzed by Zn (II) and Mn (III). Furthermore, X-ray photoelectron spectroscopy (XPS) analysis of the fresh and used catalysts revealed that the exceptional electron transfer ability of ZnO facilitated the valence transfer of Mn (III) and the transfer of electrons to the catalyst's oxygen surface, thus enhancing the catalytic efficiency. The analysis of radicals and intermediates indicates that the two main pathways for degrading 2, 4-DCP involve hydroxylation and radical attack on its aromatic ring. Practitioner Points A bimetallic ZnO/ZnMn 2 O 4 catalyst was synthesized and characterized. ZnO/ZnMn 2 O 4 can synergistically activate PMS to degrade 2, 4-DCP compared with single metal oxide. Three primary active radicals, O 2 •− , • OH, and SO 4 •− , were generated to promote the degradation. ZnO promoted electron transfer among the three species of Mn to facilitate oxidizing pollutants. Hydroxylation and radical attack on the aromatic ring of 2, 4-DCP are the two degradation pathways.
研究领域:
环境科学
·
催化化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LOSS PREVENTION IN THE PROCESS INDUSTRIES
·
2024
·
IF 3.6
Chenyang Pu,Zhi Wang,Boren Zheng,Bin Zhang
1,2,4-Triazole is a common intermediate used in the synthesis of pesticides and medicine, and has a very wide range of applications. As a kind of polynitrogen heterocycles, 1,2,4-triazole will release a lot of heat during thermal decomposition. If there is no comprehensive understanding of its pyrolysis behavior, there will be a very large thermal safety risk in the process involving 1,2,4-triazole in industrial production. At the same time, the instability of the substance may be affected by the presence of impurities, and the existing research on this aspect is quite scarce. Therefore, it is very necessary to study the effect of adding other substances on the thermal decomposition of 1,2, 4-triazole. The thermal decomposition characteristics and thermal runaway behavior of 1,2, 4-triazoles were revealed by thermodynamic experiments and various kinetic theoretical methods. According to the risk matrix, the thermal runaway danger level of 1,2, 4-triazole was determined to be level Ⅲ, which was an unacceptable risk. The results show that 1,2, 4-triazole has a huge heat release and the effect of thermal runaway is serious. In this study, three substances involved in the industrial production of fluconazole and flutriafol mixed with 1,2, 4-triazole were selected to explore their influence on the thermal decomposition of 1,2, 4-triazole under non-working conditions. DSC and ARC experiments consistently showed that these three impurities could reduce the reaction heat release of 1,2, 4-triazole to varying degrees, and confirmed that these three impurities could reduce the risk of thermal runaway of 1,2, 4-triazole to varying degrees. Among them, sodium hydride has the most outstanding performance, both in terms of reducing reaction heat release and reducing adiabatic temperature rise. The results can provide reference for loss control of 1,2,4-triazole in industrial applications.
研究领域:
化学工程
·
热力学
·
安全科学
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DOI
高影响因子
Molbank
·
2023
·Vol. 2023
·期 2
·M1664
·
IF 0.6
Lingsen Li,Jingjing Wang,Tianhao Gong,Kunming Liu,Juanhua Li
In this short note, we elaborate on a synthetic method for a new terpyridine Schiff base-bearing vanillin motif. The structure of the product was confirmed via1H and13C-NMR spectroscopy and FT-IR spectroscopy. Due to the peculiar coordination nature and optical property of the synthesized compound, this work presents a promising colorimetric sensing molecular compound.Keywords:terpyridine derivatives;Schiff base;condensation
研究领域:
化学
·
材料科学
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DOI
高影响因子
Materials Research Express
·
2020
·Vol. 7
·期 10
·105304
·
IF 1.929
Yuanji Xiao,Xinqian Yang,Xinjian Cheng,Shuangyu Xiong,Rong Chen
Among nitroaromatic compounds with similar structures, 2,4,6-trinitrophenol (TNP) exhibits strong biological toxicity and explosion hazard. Therefore, detection of TNP is of great importance. In this work, two novel fluorescent macromolecular chemosensors P1 and P2 were prepared using boron-dipyrromethene (BODIPY) as the fluorophore. After the preparation of small BODIPY molecule M1, another small molecule M2 with two aldehyde groups was synthesized by two consecutive Vilsmeier-Haack reactions. Then, M2 reacts with trans-cyclohexane-1,4-diamine and 1,4-phenylenediamine respectively to obtain two macromolecules P1 and P2 via Schiff Base forming reaction. The results suggested that P1 and P2 could recognize TNP only while other aromatic/nitroaromatic compounds cannot cause significant fluorescence intensity response. When TNP exist, these fluorescent macromolecular chemosensors produce significant fluorescence quenching. At the same time, because the electron-rich Schiff base and the electron-deficient TNP have the structural basis of chemical interaction, these two macromolecular sensors also have excellent sensitivity to TNP, and the excellent recognition limit of detection reach as low as 0.017 μM. High sensitivity and selectivity have been applied to test TNP in lake water samples as well.
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高影响因子
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