CHEMOSPHERE
·
2016
·
IF 3.698
Wenjuan Fan,Junlian Qiao,Xiaohong Guan
A new method for Cr(VI) (0.025–8.00 mg/L) determination based on the reaction of Cr(VI) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) in aqueous solutions was developed. The colorless ABTS reacted with Cr(VI) under strong acidic conditions ([H + ] = 3.0 or 6.0 M) producing a stable colored radical ABTS + , which could be measured spectrophotometrically at 415, 649 or 732 nm. The absorbance increase at these three wavelengths for ABTS + generation were all linear (less than 1.0% deviation) with respect to the amount of added Cr(VI) and the sensitivity were 1.099 × 10 5 , 3.720 × 10 4 , and 4.150 × 10 4 M −1 cm −1 of added Cr(VI) at 415, 649 or 732 nm, respectively. Cr(VI) of 0.025 mg/L below the discharge standard of drinking water could be detected with the method quantitatively and 0.002 mg/L Cr(VI) was determined qualitatively. The molar absorptivity of ABTS + generated was determined to be (3.69 ± 0.01) × 10 4 M −1 cm −1 at 415 nm and the reaction between Cr(VI) and ABTS had a stoichiometric factor of 1:3 in excess of ABTS. The absorbance of generated ABTS + was found to be stable in deionized water or wastewater and Cr(VI) spiked in wastewater could be determined accurately. The ABTS method also had a good anti-interference performance against Co(II) ions. Moreover, the ABTS method could be successfully used in the Cr(VI)-S(IV) system for Cr(VI) determination.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2022
·
IF 11.357
Yixin Huang,Jing Zou,Jinbin Lin,Haoyu Yang,Mengyun Wang,Jiawen Li,Wei Cao,Baoling Yuan,Jun Ma
The activation of peroxydisulfate (PDS) by organic compounds has attracted increasing attention. However, some inherent drawbacks including quick activator decomposition and poor anti-interference capacity limited the application of organic compound-activated PDS. It was interestingly found that 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) could act as both activator and electron shuttle for PDS activation to enhance diclofenac (DCF) degradation over a pH range of 2.0–11.0. Multiple reactive species of ABTS•+, •OH, and SO4•– were generated in the PDS/ABTS system, while only ABTS•+ and •OH directly contributed to DCF degradation. ABTS•+, generated via the reactions of ABTS with PDS, SO4•–, and •OH, was the dominant reactive species of DCF degradation. No significant decomposition of ABTS was observed in the PDS/ABTS system, and ABTS acted as both activator and electron shuttle. Four possible degradation pathways of DCF were proposed, and the toxicity of DCF decreased after treatment with the PDS/ABTS system. The PDS/ABTS system had good anti-interference capacity to common natural water constituents. Additionally, ABTS was encapsulated into cellulose to obtain ABTS@Ce beads, and the PDS/ABTS@Ce system possessed excellent performance on DCF degradation. This study proposes a new perspective to reconsider the mechanism of activating PDS with organic compounds and highlights the considerable contribution of organic radicals on contaminant removal.
研究领域:
环境科学
·
污染控制
·
化学工程
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DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2015
·Vol. 242
·期 4
·537-546
·
IF 1.559
Yang Hongli,Huang Zhongping,Chen Yin,Zhang Changjun,Ye Mengdie,Wang Lili
Propolis, a well-known folk medicine in the world, has drawn growing attention due to the various bioactivities. An on-line method of coupling HPLC with 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) cationic radical scavenging detection (HPLC–ABTS) was developed to screen the single polyphenol possessing ABTS cationic radical scavenging activity in ethanol extracts of Chinese propolis (EECP). Ten common polyphenols were selected from the active fingerprints of ten EECP samples obtained from different provinces of China by the on-line HPLC–ABTS method and quantified by Trolox equivalent. Their contributions to the antioxidant property of EECP were compared. Furthermore, Pearson’s correlation analysis was applied to determine whether there was a correlation between the antioxidant activity of individual polyphenol and total antioxidant activity of EECP. The results indicated that the composition of polyphenols in ten EECP samples obtained from China was similar. But the different polyphenols contributed in different way. Caffeic acid, kaempferol, and galangin simultaneously were the most prominent contributors to the antioxidant properties of ten EECP. The contributions of pinobanksin 3-acetate, caffeic acid phenethyl ester (CAPE), and caffeic acid cinnamyl ester varied largely with the different geographical regions. They, except galangin, significantly correlated with the antioxidant property of propolis. However, pinobanksin, pinocembrin, chrysin, p -coumaric acid, and ferulic acid rarely contributed to the antioxidant property of Chinese propolis. Therefore, the on-line HPLC–ABTS method was convenient, effective, and comprehensive to evaluate the contributions of polyphenols to the antioxidant property for Chinese propolis.
研究领域:
植物化学
·
药理学
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产品关联
DOI
高影响因子
Natural Product Communications
·
2015
·
IF 0.906
Jian-Wei Dong,Le Cai,Yun Xing,Jing Yu,Zhong-Tao Ding
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS•+) is a stable free radical frequently used for estimating the total antioxidant capacity (TAC) of natural products. The existing methods for ABTS•+ radical-scavenging activity assays are diverse in pre-diluting solvents and reaction time, which lead to errors in the TAC estimations. To develop an effective and universal method for estimating the ABTS•+ capacity accurately and reasonably, five pre-dilution solvents [methanol, ethanol, phosphate buffer (Na2HPO4-NaH2PO4, 200 mM, pH = 7.4), PBS buffer (Na2HPO4-NaH2PO4-NaCl, 200 mM, pH = 7.4), and distilled water] and different reaction times were investigated in ABTS•+ assays of five typical antioxidants. The results showed that the solvent effects were very significant. When using different pre-diluting solvents, different detection wavelengths should be selected. ABTS-+ assay could be measured within 2–10 min to obtain a rough result, which was mostly 6 min in the literature. However, full and accurate evaluation of antioxidant reactivity rather than capacity requires recording ABTS•+ loss continuously during the whole reaction period. The present study makes a recommendation for estimating the ABTS•+ capacity accurately and reasonably.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
ACS ES&T Water
·
2024
·
IF 4.8
Xiao-Na Zhao,Zhuang-Song Huang,Lu Wang,Zhi Gao,Yang Li,Qing-Liang Liu,Yu-Lei Liu,Jun Ma
Colorimetric methods using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as a chromogenic reagent are widely used for oxidant detection in water samples. However, the coexisting natural organic matter, especially those having phenolic hydroxyl groups, can consume the formed ABTS·+ radical, leading to a reduced measured concentration using the ABTS method. Using phenols as model substances, the rate constants between ABTS·+ and deprotonated phenols could reach as high as 107∼109 M–1 s–1, approximately 4–6 orders of magnitude higher than those with protonated phenols. These reaction rates could decrease with an increase in ABTS concentration. Lowering the chromogenic pH and increasing the initial ABTS concentration can reduce the consumption of ABTS·+ by matrix components, but does not fully eliminate the reductive interference caused by para- or ortho-phenolic hydroxyl groups. Similar measurement deviations of the ABTS method were also observed during the analysis of real water samples. Relatively, utilizing 3,3′,5,5′-tetramethylbenzidine as the chromogenic reagent, the TMB method exhibits stronger anti-interference performance toward matrix components in both synthetic solutions and natural waters, making it suitable for the quantitative analysis of multiple oxidants. This work investigates the deviation characteristics of the ABTS method and provides several suggestions for the accurate and sensitive determination of oxidants in complex aqueous environments.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 19
·7973-7977
·
IF 1.938
Yingfeng Qin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
A homogeneous label-free colorimetric sensing strategy for bleomycin (BLM) assay was developed on the basis of BLM enhanced Fe(II)–H2O2–ABTS reaction. Fe(II) exhibits a catalytic effect on H2O2-mediated oxidation of ABTS by Fenton chemistry. BLM·Fe(II) complex possessed a significantly higher catalytic ability than Fe(II) probably because of the further formation of more active “activated oxo-iron-bleomycin complex”. The present method was convenient without any complicated chemical synthesis, modification, or tedious experimental procedures. Analysis results can be seen with the naked eye and monitored by UV-vis spectra. The peak absorbance exhibits a linear relationship with BLM concentrations from 25 nM to 1 μM, and a detection limit of 16 nM can be achieved. The designed colorimetric strategy may provide a promising alternative for BLM detection in clinical samples.
研究领域:
分析化学
·
生物医学
·
临床检验
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产品关联
DOI
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Huahua Cai,Xin Liu,Jing Zou,Junyang Xiao,Baoling Yuan,Fei Li,Qingfeng Cheng
In this study, a new spectrophotometric method was proposed for the measurement of hydrogen peroxide (H 2 O 2 ) in aqueous solutions. The method was based on the peroxidase (POD)-catalyzed reaction in which 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was oxidized to form the stable green radical (ABTS + ). The generated ABTS + could be determined spectrophotometrically. The absorbance of the generated ABTS + at 415 nm, 650 nm, 732 nm and 820 nm were linear with H 2 O 2 concentrations in the range of 0–40 μM. The sensitivities of the proposed ABTS method for H 2 O 2 determination at 415 nm, 650 nm, 732 nm and 820 nm were 6.29 × 10 4 M −1 cm −1 , 2.00 × 10 4 M −1 cm −1 , 2.54 × 10 4 M −1 cm −1 and 1.89 × 10 4 M −1 cm −1 , respectively. The oxidation of ABTS to generate ABTS + in POD-catalyzed H 2 O 2 system at pH 6.0 was so fast that the determination time of the ABTS method was as short as 0.5 min. The stoichiometry of the reaction of H 2 O 2 and ABTS in the presence of POD was calculated as nearly 1:2 (1:1.92). The residual absorbance of the generated ABTS + was also found to be stable within 30 min in natural waters. Low H 2 O 2 concentration in rainwater could be both measured by the ABTS method and the DPD method with high accuracy, however, H 2 O 2 concentration in wastewater contained rhodamine B could only be accurately measured with the ABTS method at 732 nm. Moreover, waste solutions after H 2 O 2 analysis with the proposed ABTS method were non-hazardous towards E. coli .
研究领域:
分析化学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2024
·
IF 5.3
Qinhai Xu,Kang Li,Peng Wang
The reaction mechanism of p -hydroxystyryl-substituted BODIPY (BOH) with two oxidative cations, namely ABTS •+ and Fe 3+ , was investigated in a water–ethanol mixed solution and in liposome suspensions, respectively, using different spectroscopic methods. In solution, the oxidation of BOH (with orange fluorescence) by the two cations occurred at the ethylene group (C C) locating between the dipyrrole and phenol groups and resulted in conjugation-truncated products exhibiting characteristic green fluorescence emission. In heterogeneous small unilamellar vesicles (SUV), water soluble ABTS •+ was evidenced to oxidize BOH embedded in the lipid bilayers of SUV, while Fe 3+ did not. The lack of reaction between Fe 3+ and BOH was attributed to the complexation between Fe 3+ and the phenolic hydroxyl group of BOH on the surface of the SUV. The reaction kinetics results indicated that, in homogeneous solution, the oxidation rate of Fe 3+ was three orders of magnitude slower than that of ABTS •+ for BOH. The location and orientation of BOH within the SUV were discussed based on the reaction phenomena. BOH could be as a good antioxidant fluorescent prober for ABTS •+ detection with a detection limit of 1.5 * 10 −7 M and a linear rang of 0–4.93 μM. What’s more, the amphiphilic BOH dispersed in the round GUV (BOH + GUV) could show the bright red fluorescence. This research suggests the significant potential of BOH as an antioxidant fluorescent probe for in situ bioimaging.
研究领域:
化学
·
生物化学
·
医学
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产品关联
DOI
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 6
·920
·
IF 2.861
Youxun Liu,Yuanyuan Geng,Mingyang Yan,Juan Huang
The successful encapsulation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules. ABTS@MIL-100(Fe) was then evaluated as an immobilized laccase mediator for dye removal using indigo carmine (IC) as a model dye. Through the application of laccase in combination with a free (ABTS) or immobilized (ABTS@MIL-100(Fe)) mediator, decolorization yields of 95% and 94%, respectively, were obtained for IC after 50 min. In addition, following seven reuse cycles of ABTS@MIL-100(Fe) for dye treatment, a decolorization yield of 74% was obtained. Dye decolorization occurred through the breakdown of the chromophoric group by the Laccase/ABTS@MIL-100(Fe) system, and a catalytic mechanism was proposed. We therefore expect that the stability, reusability, and validity of ABTS@MIL-100(Fe) as a laccase mediator potentially render it a promising tool for dye removal, in addition to reducing the high running costs and potential toxicity associated with synthetic mediators.Keywords:laccase;ABTS;metal-organic framework;mediator;decolorization
研究领域:
生物化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Chemical Engineering Journal Advances
·
2022
·
IF 0
Na Yuan,Hongchao Li,Jieshu Qian
Determination of the concentration of peracetic acid (PAA) in the presence of H 2 O 2 is a great challenging problem due to the similar chemical behaviors of the two species. In this study, we developed a new method for simultaneous measurement of PAA (0.5 – 10.0 μM) and total peroxides (i.e., PAA and H 2 O 2 with a molar ratio of 10:6.5), based on the catalytic oxidation of ABTS by separately using Co(II) and peroxidase (POD) as the catalysts. Owing to the unique catalytic activity of Co(II) towards PAA, the coexistent H 2 O 2 exhibits negligible effect on the determination of PAA. The value of absorbance at 415 nm increases linearly with increasing the concentration of PAA or total peroxides, and the sensitivities for PAA and total peroxides are as high as 5.31 × 10 4 and 9.07 × 10 4 M −1 cm −1 , respectively. In addition, the proposed ABTS method shows excellent feasibility and applicability, which make it suitable to be employed for detection in complicated water matrices.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
Journal of Chemistry
·
2015
·Vol. 2015
·期 1
·670194
·
IF 0.772
Youxun Liu,Mingyang Yan,Yuanyuan Geng,Juan Huang
Efficient reuse and regeneration of spent mediators are highly desired for many of the laccases’ biotechnology applications. This investigation demonstrates that a redox mediator 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) covalently attached to silica nanoparticles (SNPs) effectively mediated dye decolorization catalyzed by laccase. Characteristics of ABTS-modified silica nanoparticles (ABTS-SNPs) were researched by scanning electron microscopy and Fourier-transformed infrared spectroscopy. When ABTS and ABTS-SNPs were used as laccase mediators, the decolorization yields of 96 and 95% were, respectively, obtained for indigo carmine dye. The results suggest that ABTS immobilized on SNPs can be used as laccase mediators as they retain almost the same efficiency as the free ABTS. The oxidized ABTS-SNPs were regenerated by their reduction reaction with ascorbic acid. Decolorization efficiency of regenerated ABTS-SNPs and their initial forms were found to be almost equivalent. Six reuse cycles for spent ABTS-SNPs were run for the treatment of indigo carmine, providing decolorization yields of 96–77%. Compared with free mediator, the immobilized mediators have the advantage of being easily recovered, regenerated, and reused making the whole process environmentally friendly.
研究领域:
环境生物技术
·
纳米生物技术
·
酶工程
·
污染物降解
·
绿色化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jing-Wen Hao,Wei-Ting Wang,Nai-Dong Chen,Yan Shen
The novel application of a combined screening method, utilizing AAPH, ABTS, and FRAP assays with HPLC, is first used for screening natural antioxidants in Green Calyx plums (GCP). This method was assumed that the peak areas of compounds with antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH, ABTS, and FRAP. The thirteen screened antioxidants can be identified by Q-TOF-MS/MS. kaempferol-3-O-rutinoside, narcissoside, and astragalin were first reported in GCP. respectively. The thirteen screened antioxidants were quantified simultaneously at their maximum absorption wavelengths, with concentrations ranging from 0.61 to 79.30 mg/g across three distinct flowering stages. Furthermore, the in vitro activity and molecular docking of oxidative stress-related enzyme xanthine oxidase (XOD) were utilized to evaluate the antioxidant capacity of the screened compounds. Consequently, our method effectively screens for antioxidants within complex mixtures, positioning GCP as a promising reservoir of natural bioactive compounds.
研究领域:
植物化学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）