ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2022
·
IF 11.357
Yixin Huang,Jing Zou,Jinbin Lin,Haoyu Yang,Mengyun Wang,Jiawen Li,Wei Cao,Baoling Yuan,Jun Ma
The activation of peroxydisulfate (PDS) by organic compounds has attracted increasing attention. However, some inherent drawbacks including quick activator decomposition and poor anti-interference capacity limited the application of organic compound-activated PDS. It was interestingly found that 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) could act as both activator and electron shuttle for PDS activation to enhance diclofenac (DCF) degradation over a pH range of 2.0–11.0. Multiple reactive species of ABTS•+, •OH, and SO4•– were generated in the PDS/ABTS system, while only ABTS•+ and •OH directly contributed to DCF degradation. ABTS•+, generated via the reactions of ABTS with PDS, SO4•–, and •OH, was the dominant reactive species of DCF degradation. No significant decomposition of ABTS was observed in the PDS/ABTS system, and ABTS acted as both activator and electron shuttle. Four possible degradation pathways of DCF were proposed, and the toxicity of DCF decreased after treatment with the PDS/ABTS system. The PDS/ABTS system had good anti-interference capacity to common natural water constituents. Additionally, ABTS was encapsulated into cellulose to obtain ABTS@Ce beads, and the PDS/ABTS@Ce system possessed excellent performance on DCF degradation. This study proposes a new perspective to reconsider the mechanism of activating PDS with organic compounds and highlights the considerable contribution of organic radicals on contaminant removal.
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高影响因子
CHEMOSPHERE
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2016
·
IF 3.698
Wenjuan Fan,Junlian Qiao,Xiaohong Guan
A new method for Cr(VI) (0.025–8.00 mg/L) determination based on the reaction of Cr(VI) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) in aqueous solutions was developed. The colorless ABTS reacted with Cr(VI) under strong acidic conditions ([H + ] = 3.0 or 6.0 M) producing a stable colored radical ABTS + , which could be measured spectrophotometrically at 415, 649 or 732 nm. The absorbance increase at these three wavelengths for ABTS + generation were all linear (less than 1.0% deviation) with respect to the amount of added Cr(VI) and the sensitivity were 1.099 × 10 5 , 3.720 × 10 4 , and 4.150 × 10 4 M −1 cm −1 of added Cr(VI) at 415, 649 or 732 nm, respectively. Cr(VI) of 0.025 mg/L below the discharge standard of drinking water could be detected with the method quantitatively and 0.002 mg/L Cr(VI) was determined qualitatively. The molar absorptivity of ABTS + generated was determined to be (3.69 ± 0.01) × 10 4 M −1 cm −1 at 415 nm and the reaction between Cr(VI) and ABTS had a stoichiometric factor of 1:3 in excess of ABTS. The absorbance of generated ABTS + was found to be stable in deionized water or wastewater and Cr(VI) spiked in wastewater could be determined accurately. The ABTS method also had a good anti-interference performance against Co(II) ions. Moreover, the ABTS method could be successfully used in the Cr(VI)-S(IV) system for Cr(VI) determination.
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Natural Product Communications
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2015
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IF 0.906
Jian-Wei Dong,Le Cai,Yun Xing,Jing Yu,Zhong-Tao Ding
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS•+) is a stable free radical frequently used for estimating the total antioxidant capacity (TAC) of natural products. The existing methods for ABTS•+ radical-scavenging activity assays are diverse in pre-diluting solvents and reaction time, which lead to errors in the TAC estimations. To develop an effective and universal method for estimating the ABTS•+ capacity accurately and reasonably, five pre-dilution solvents [methanol, ethanol, phosphate buffer (Na2HPO4-NaH2PO4, 200 mM, pH = 7.4), PBS buffer (Na2HPO4-NaH2PO4-NaCl, 200 mM, pH = 7.4), and distilled water] and different reaction times were investigated in ABTS•+ assays of five typical antioxidants. The results showed that the solvent effects were very significant. When using different pre-diluting solvents, different detection wavelengths should be selected. ABTS-+ assay could be measured within 2–10 min to obtain a rough result, which was mostly 6 min in the literature. However, full and accurate evaluation of antioxidant reactivity rather than capacity requires recording ABTS•+ loss continuously during the whole reaction period. The present study makes a recommendation for estimating the ABTS•+ capacity accurately and reasonably.
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高影响因子
RSC Advances
·
2021
·Vol. 11
·期 41
·25646-25652
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IF 3.361
Qian-Hui Shen,Qi Huang,Ju-Ying Xie,Kun Wang,Zheng-Ming Qian,De-Qiang Li
In the present study, a simple and efficient approach based on the online extraction-high performance liquid chromatography coupled with ABTS antioxidant assay (OLE-HPLC-ABTS) was established to quickly and directly analyze the antioxidants in S. baumii. Through this system, the HPLC mobile phase via a guard column packed with a S. baumii sample was used for online extraction (OLE). The separation was performed on an Agilent Poroshell EC-C18 column with a gradient elution using 0.1% formic acid (A) and 0.1% formic acid–acetonitrile (B) as mobile phase systems and detected at a wavelength of 254 nm. Then, the separated compounds were reacted with the antioxidant solution (ABTS), and the response was recorded at a wavelength of 400 nm. The developed analytical method was successfully applied to S. baumii samples, and eight antioxidants were identified. The established system integrated the online extraction, separation and online antioxidant detection, which is rapid, efficient, and suitable for the rapid screening of antioxidant compounds from solid sample mixtures.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
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IF 9.8
Jiaxin Xu,Jing Zou,Jianying Wu,Huiping Zeng,Yixin Huang,Jingxin Yang,Chunming Gong,Siying Chen,Jun Ma
Chlorination, one of the most common oxidation strategies, performed limited degradation capacity towards many emerging organic contaminants under neutral pH conditions. In this study, 2,2′-azinobis(3-ethylbenzothiazoline)-6-sulfonate (ABTS) was discovered to possess an outstanding activation property towards free available chlorine (FAC) during the chlorination of diclofenac (DCF) among pH 6.0–9.5. ABTS radical (ABTS •+ ) primarily accounted for the elimination of DCF in the ABTS/FAC system, although hydroxyl radicals , reactive chlorine species, and singlet oxygen were also generated via the self-decomposition of FAC. ABTS acted as the electron shuttle to degrade DCF in the ABTS/FAC system, where ABTS was firstly oxidized by FAC to ABTS •+ via single electron transfer , and followed by the elimination of DCF with the generated ABTS •+ . Eight DCF degradation intermediates were identified by LC/Q-TOF/MS, and four DCF degradation pathways were proposed. Real water bodies, humic acid , and the coexistent anions of Cl − , HCO 3 − , NO 3 − , and SO 4 2− performed negligible influence on DCF removal in ABTS/FAC system. ABTS/FAC system was much superior to sole chlorination in terms of toxicity reduction and anti-interference capacity. Overall, this study innovatively introduced ABTS as the electron shuttle to enhance the oxidative capacity of FAC under neutral pH conditions and provided a new insight that the ABTS-like organic/synthetic components might play an important role in degrading emerging organic contaminants by chlorination in water treatment .
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ACS ES&T Water
·
2024
·
IF 4.8
Xiao-Na Zhao,Zhuang-Song Huang,Lu Wang,Zhi Gao,Yang Li,Qing-Liang Liu,Yu-Lei Liu,Jun Ma
Colorimetric methods using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as a chromogenic reagent are widely used for oxidant detection in water samples. However, the coexisting natural organic matter, especially those having phenolic hydroxyl groups, can consume the formed ABTS·+ radical, leading to a reduced measured concentration using the ABTS method. Using phenols as model substances, the rate constants between ABTS·+ and deprotonated phenols could reach as high as 107∼109 M–1 s–1, approximately 4–6 orders of magnitude higher than those with protonated phenols. These reaction rates could decrease with an increase in ABTS concentration. Lowering the chromogenic pH and increasing the initial ABTS concentration can reduce the consumption of ABTS·+ by matrix components, but does not fully eliminate the reductive interference caused by para- or ortho-phenolic hydroxyl groups. Similar measurement deviations of the ABTS method were also observed during the analysis of real water samples. Relatively, utilizing 3,3′,5,5′-tetramethylbenzidine as the chromogenic reagent, the TMB method exhibits stronger anti-interference performance toward matrix components in both synthetic solutions and natural waters, making it suitable for the quantitative analysis of multiple oxidants. This work investigates the deviation characteristics of the ABTS method and provides several suggestions for the accurate and sensitive determination of oxidants in complex aqueous environments.
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Journal of Chemistry
·
2015
·Vol. 2015
·期 1
·670194
·
IF 0.772
Youxun Liu,Mingyang Yan,Yuanyuan Geng,Juan Huang
Efficient reuse and regeneration of spent mediators are highly desired for many of the laccases’ biotechnology applications. This investigation demonstrates that a redox mediator 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) covalently attached to silica nanoparticles (SNPs) effectively mediated dye decolorization catalyzed by laccase. Characteristics of ABTS-modified silica nanoparticles (ABTS-SNPs) were researched by scanning electron microscopy and Fourier-transformed infrared spectroscopy. When ABTS and ABTS-SNPs were used as laccase mediators, the decolorization yields of 96 and 95% were, respectively, obtained for indigo carmine dye. The results suggest that ABTS immobilized on SNPs can be used as laccase mediators as they retain almost the same efficiency as the free ABTS. The oxidized ABTS-SNPs were regenerated by their reduction reaction with ascorbic acid. Decolorization efficiency of regenerated ABTS-SNPs and their initial forms were found to be almost equivalent. Six reuse cycles for spent ABTS-SNPs were run for the treatment of indigo carmine, providing decolorization yields of 96–77%. Compared with free mediator, the immobilized mediators have the advantage of being easily recovered, regenerated, and reused making the whole process environmentally friendly.
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Colloid and Interface Science Communications
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2023
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IF 4.5
Chengyang Ban,Hanzhu Shi,Qiong Ding,Bin Wu,Ru Xia,Jiasheng Qian,Ming Cao
In this study, Fe/ABTS/PVP nanocomplexes (named FAPs) as photothermal-reinforced catalytic antibacterial agents were prepared using the coordination-induced assembly of Fe (III), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and polyvinylpyrrolidone (PVP). The redox pairs of Fe(II)/Fe(III) in FAPs can catalyze the H 2 O 2 to hydroxyl radicals (•OH) through Fenton reactions, thereby killing effect on bacteria. The photothermal effect of ABTS •+ in FAPs stimulated by near infrared (NIR) irradiation can further improve the catalytic effect, thereby generating more •OH and achieving better antibacterial activity. What's more, the reduction reaction between glutathione (GSH) and ABTS •+ results in the depletion of the GSH level, the redox pair of Fe(II)/Fe(III) is also beneficial in the consumption of GSH. This dual depletion of GSH further expands the intracellular oxidative stress. Thus, the integrated function of photothermal effect, catalytic property, and dual depletion of GSH by FAPs provides a new strategy for fighting against bacterial infections.
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高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Huahua Cai,Xin Liu,Jing Zou,Junyang Xiao,Baoling Yuan,Fei Li,Qingfeng Cheng
In this study, a new spectrophotometric method was proposed for the measurement of hydrogen peroxide (H 2 O 2 ) in aqueous solutions. The method was based on the peroxidase (POD)-catalyzed reaction in which 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was oxidized to form the stable green radical (ABTS + ). The generated ABTS + could be determined spectrophotometrically. The absorbance of the generated ABTS + at 415 nm, 650 nm, 732 nm and 820 nm were linear with H 2 O 2 concentrations in the range of 0–40 μM. The sensitivities of the proposed ABTS method for H 2 O 2 determination at 415 nm, 650 nm, 732 nm and 820 nm were 6.29 × 10 4 M −1 cm −1 , 2.00 × 10 4 M −1 cm −1 , 2.54 × 10 4 M −1 cm −1 and 1.89 × 10 4 M −1 cm −1 , respectively. The oxidation of ABTS to generate ABTS + in POD-catalyzed H 2 O 2 system at pH 6.0 was so fast that the determination time of the ABTS method was as short as 0.5 min. The stoichiometry of the reaction of H 2 O 2 and ABTS in the presence of POD was calculated as nearly 1:2 (1:1.92). The residual absorbance of the generated ABTS + was also found to be stable within 30 min in natural waters. Low H 2 O 2 concentration in rainwater could be both measured by the ABTS method and the DPD method with high accuracy, however, H 2 O 2 concentration in wastewater contained rhodamine B could only be accurately measured with the ABTS method at 732 nm. Moreover, waste solutions after H 2 O 2 analysis with the proposed ABTS method were non-hazardous towards E. coli .
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2015
·Vol. 242
·期 4
·537-546
·
IF 1.559
Yang Hongli,Huang Zhongping,Chen Yin,Zhang Changjun,Ye Mengdie,Wang Lili
Propolis, a well-known folk medicine in the world, has drawn growing attention due to the various bioactivities. An on-line method of coupling HPLC with 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) cationic radical scavenging detection (HPLC–ABTS) was developed to screen the single polyphenol possessing ABTS cationic radical scavenging activity in ethanol extracts of Chinese propolis (EECP). Ten common polyphenols were selected from the active fingerprints of ten EECP samples obtained from different provinces of China by the on-line HPLC–ABTS method and quantified by Trolox equivalent. Their contributions to the antioxidant property of EECP were compared. Furthermore, Pearson’s correlation analysis was applied to determine whether there was a correlation between the antioxidant activity of individual polyphenol and total antioxidant activity of EECP. The results indicated that the composition of polyphenols in ten EECP samples obtained from China was similar. But the different polyphenols contributed in different way. Caffeic acid, kaempferol, and galangin simultaneously were the most prominent contributors to the antioxidant properties of ten EECP. The contributions of pinobanksin 3-acetate, caffeic acid phenethyl ester (CAPE), and caffeic acid cinnamyl ester varied largely with the different geographical regions. They, except galangin, significantly correlated with the antioxidant property of propolis. However, pinobanksin, pinocembrin, chrysin, p -coumaric acid, and ferulic acid rarely contributed to the antioxidant property of Chinese propolis. Therefore, the on-line HPLC–ABTS method was convenient, effective, and comprehensive to evaluate the contributions of polyphenols to the antioxidant property for Chinese propolis.
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CHEMOSPHERE
·
2020
·
IF 5.778
Yifei Leng,Jianguo Bao,Henglin Xiao,Dandan Song,Jiangkun Du,Sanjeeb Mohapatra,David Werner,Jun Wang
The threat of antibiotics in the environment causing antibiotics resistance is a global health concern. Enzymes catalyze pollutant transformations, and how commercially available enzymes like horseradish peroxidase (HRP), with or without a redox mediator, may be used to degrade antibiotics in water treatment is of great interest. This work demonstrates tetracycline transformation by HRP, and how it is significantly enhanced by free radicals created from the mediator 2,2-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Water temperature and pH strongly influence the tetracycline removal rate due to their correlation with the enzyme activity , abundance and stability of ABTS •+ . Four transformation products were identified in the pure HRP system using a liquid chromatography tandem mass spectrometry hybrid quadrupole-orbitrap mass spectrometer system. Addition of 25 μmol L −1 ABTS not only accelerated the degradation of tetracycline, but also expanded the range of degradation pathways. Potential tetracycline transformation pathways are proposed based on these observations, which include a range of mechanisms such as hydroxylation, demethylation, dehydration, decarbonylation and secondary alcohol oxidation. Despite of decreased efficiency, the HRP/ABTS system was able to degrade tetracycline in a domestic wastewater treatment plant effluent matrix, which demonstrates the potential of the system to be utilized in wastewater treatment.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Wei Yang,Ruicheng Xu,Feifei Mu,Jérome Chauvin,Serge Cosnier,Xue-Ji Zhang,Dan Shan
Uncontrolled diffusion and instability of redox mediators remain major challenges limiting the durability and fidelity of electrochemical biosensors. Here, we report a biomimetic strategy that encapsulates 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) within phospholipid vesicles to construct a vesicle-confined electron relay platform for hydrogen peroxide (H 2 O 2 ) sensing. The confined aqueous microenvironment preserves the redox activity of ABTS, prevents mediator leakage and degradation, and enables efficient electron shuttling with horseradish peroxidase (HRP). Consequently, the biosensor achieves ultrasensitive electrochemical detection (LOD: 2.53 nM), retains >70 % of its activity after 30 days at 4 °C, and endures over 500 CV cycles without signal loss. In parallel, a complementary chromogenic output provides an intuitive visual response in the micromolar range, underscoring the synergistic advantages of recyclable electrochemical relays versus one-off colorimetric products. Importantly, the platform enabled intracellular H 2 O 2 monitoring in living cells, validating its biocompatibility and robustness under biologically relevant conditions. This organelle-inspired, dual-mode sensing architecture offers a generalizable framework for stabilizing redox mediators through nanoconfinement and opens new avenues for durable, field-deployable point-of-care biosensing.
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危险品化学品经营许可证（带存储）