ANALYTICA CHIMICA ACTA
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2024
·
IF 5.7
Yuping Zeng,Xiangyu Gan,Zhourui Xu,Xiaoxiang Hu,Chenxuan Hu,Hanbin Ma,Hangjia Tu,Bao Chai,Chengbin Yang,Siyi Hu,Yujuan Chai
Background Sensitive and rapid antigen detection is critical for the diagnosis and treatment of infectious diseases, but conventional ELISAs including chemiluminescence-based assays are limited in sensitivity and require many operation steps. Fluorescence immunoassays are fast and convenient but often show limited sensitivity and dynamic range. Results To address the need, an aggregation-induced emission fluorgens (AIEgens) enhanced i mmunofluorescent assay with beads-based quantification on the digital microfluidic (DMF) platform was developed. Portable DMF devices and chips with small electrodes were fabricated, capable of manipulating droplets within 100 nL and boosting the reaction efficiency. AIEgen nanoparticles (NPs) with high fluorescence and photostability were synthesized to enhance the test sensitivity and detection range. The integration of AIEgen probes, transparent DMF chip design, and the large magnetic beads (10 μm) as capture agents enabled rapid and direct image-taking and signal calculation of the test result. The performance of this platform was demonstrated by point-of-care quantification of SARS-CoV-2 nucleocapsid (N) protein. Within 25 min, a limit of detection of 5.08 pg mL −1 and a limit of quantification of 8.91 pg mL −1 can be achieved using <1 μL sample. The system showed high reproducibility across the wide dynamic range (10–10 5 pg mL −1 ), with the coefficient of variance ranging from 2.6% to 9.8%. Significance This rapid, sensitive AIEgens-enhanced immunofluorescent assay on the DMF platform showed simplified reaction steps and improved performance, providing insight into the small-volume point-of-care testing of different biomarkers in research and clinical applications.
研究领域:
生物医学工程
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分析化学
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分子诊断
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DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Yize Lin,Yan Tan,Rong Deng,Li Gong,Xiaoshan Feng,Zhongqi Cai,Yanxian He,Longbao Feng,Biao Cheng,Yi Chen
Chronic wounds are characterized with excessive biofluid and persistent infection. Therefore, there is an urgent desire to develop a multifunctional wound dressing that can meet the extreme requirements including effective antibacterial and powerful wound microenvironment regulation and protection function to promote wounds heal quickly. In this study, a multifunctional composite dressing (HA-AMP/SF/Alg/Rb-BG-AIEgens) was synthesized by combining a mesoporous bioactive glass framework loaded with AIEgens (Rb-BG-AIEgens) with cross-linked antimicrobial peptide grafted hyaluronic acid (HA-AMP), sodium alginate (Alg), and silk fibroin (SF). It is important to note that the Rb-BG-AIEgens can achieve real-time and sensitive bacterial detection. HA-AMP can achieve broad spectrum antibacterial and avoid the residue of drug-resistant bacteria. The HA-AMP/SF/Alg/Rb-BG-AIEgens dressing can up-regulate related proliferative proteins, thereby promoting regeneration of tissue and the rapid healing of chronic wounds. With good biocompatibility and antibacterial ability, HA-AMP/SF/Alg/Rb-BG-AIEgens dressing has great potential to become a next generation wound dressing for clinical biological fluid management and chronic bacterial infection treatment.
研究领域:
生物医学工程
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材料科学
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伤口愈合
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感染控制
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DOI
PubMed
高影响因子
Polymer Chemistry
·
2018
·Vol. 9
·期 30
·4150-4160
·
IF 4.927
Qian Li,Xiao Li,Zhongying Wu,Yuhao Sun,Jianglin Fang,Dongzhong Chen
Tetraphenylethylene (TPE) is one of the well-known typical aggregation-induced emission (AIE) luminogens (AIEgens) and TPE-containing polymers possess excellent processing properties which facilitates the fabrication of various AIE systems and devices. For AIE active side-chain polymers, increasing the fluorescence quantum yield through suitable molecular design is very appealing. In this work, we present the well-controlled synthesis of a series of TPE polyacrylate side-chain polymers of Pm with short spacers with methylene number m = 0–5 via RAFT polymerization. All the obtained polymers exhibit typical and strong AIE characteristics in THF/H2O solution mixtures and in solid state films. In particular, greatly enhanced fluorescence emission is achieved with reduced spacer lengths, and further significant improvement achieved through annealing, which is ascribed to the restriction of intramolecular motions (RIM) and the blocking of the non-radiative channels due to overcrowding and confinement thanks to the strong positive coupling effect and the jacketing effect of side-chain polymers with very short spacers. The quantum yields of 34.4%, 32.0% and 31.4%, respectively, for the polymer films P0, P1 and P2 are several times higher than those of homologous polymers with longer spacers reported in the literature. Based on these TPE side-chain polymers of very short spacers, a simple, economic and visual method for detection of the explosive 2,4,6-trinitrotoluene (TNT) is demonstrated. Therefore, a facile approach and feasible strategy of simply adopting very short spacers for side-chain AIE active polymer preparation to significantly enhance the luminescence efficiency has been properly established and proposed, which may provide guidance and have a wealth of implications for the rational design and preparation of highly efficient luminescent AIE polymer materials.
研究领域:
材料科学
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高分子化学
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分析化学
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纳米技术
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DOI
高影响因子
Journal of Materials Chemistry B
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2021
·Vol. 9
·期 15
·3317-3325
·
IF 6.331
Lixiu Chen,Bin Xia,Bing Yan,Jianhua Liu,Zhaohua Miao,Yan Ma,Jinchen Wang,Hu Peng,Tao He,Zhengbao Zha
Multifunctional contrast-enhanced agents suitable for application in surgical navigation by taking advantage of the merits of their diverse imaging modalities at different surgical stages are highly sought-after. Herein, an amphipathic polymer composed of aggregation-induced emission fluorogens (AIEgens) and Gd3+ chelates was successfully synthesized and assembled into ultrasound responsive microbubbles (AIE-Gd MBs) to realize potential tri-modal contrast-enhanced ultrasound (US) imaging, magnetic resonance imaging (MRI), and AIEgen-based fluorescence imaging (FI) during the perioperative period. Through ultrasound targeted microbubble destruction (UTMD) and cavitation effect, the as-prepared AIE-Gd MBs went through a MBs-to-nanoparticles (NPs) conversion, which not only resulted in targeted accumulation in tumor tissues but also led to stronger fluorescence being exhibited due to the more aggregated AIE-Gd molecules in the NPs. As a proof-of-concept, our work proposes a strategy of US-lit-up AIEgens in tumors which could offer a simple and powerful tool for surgical navigation in the future.
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高影响因子
Small
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2024
·
IF 13
Jun Zhu,Leyuan Tian,Dingyuan Yan,Dong Wang,Ben Zhong Tang
Exploiting single molecular species synchronously affording powerful second near-infrared (NIR-II) fluorescence, superior photoacoustic output, prominent reactive oxygen species generation, and satisfactory photothermal conversion is supremely appealing for phototheranostics, yet remains formidably challenging. In this work, electron donor/π-bridge engineering is implemented on the basis of 6,7-di(thiophen-2-yl)-[1,2,5]thiadiazolo[3,4- g ]quinoxaline moiety. The optimal molecule, namely TPATO-TTQ, is demonstrates to exhibit those notable features requested by exceptional phototheranostics, which are systematically elucidated through the depictions of excited-state energy dissipation pathways and the influence of intramolecular motion on the photophysical properties, with assistances of quantum chemical calculation and molecular dynamic simulation. By utilizing TPATO-TTQ nanoparticles, unprecedented performance on NIR-II fluorescence-photoacoustic-photothermal trimodal imaging-navigated type I photodynamic-photothermal synergistic therapy to orthotopic breast cancer is authenticates by the precise tumor diagnosis and complete tumor ablation.
研究领域:
生物医学工程
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肿瘤学
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光学成像
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DOI
PubMed
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 2
·154
·
IF 4.034
Guifang Wang,Jing Wang,Linlin Zhao,Qiang Zhang,Yan Lu
In the present work, we describe a facile and general method of fabricating fluorescent inorganic nanoparticles with diverse shapes for cell imaging application. The hematite (α-Fe2O3) nanoparticles (HNPs) with three different shapes (i.e., spindle shape, ellipsoidal shape and quasi-spherical shape) were first prepared as model systems in consideration of good biocompatibility and the controllable morphology of α-Fe2O3. Three fluorescent HNPs with different shapes were readily achieved via one-pot sol-gel reaction of AIE luminogen-functionalized siloxane (AIEgen-Si(OCH3)3) and TEOS in the presence of PVP-stabilized HNPs. Due to the fluorescence originating from the thin AIEgens-contained SiO2shell around the HNPs, their photoluminescent intensities can be tuned by changing the concentrations of TEOS and AIEgen-Si(OCH3)3in feed prior to the sol-gel reaction. When the as-prepared fluorescent products were dispersed in water, they gave intense green light emission upon excitation at 360 nm with relatively high fluorescence quantum yield. Further, fluorescent HNPs exhibited low cytotoxicity and excellent photostability and, thus, were used as optical probes to preliminarily explore the effect of nanoparticle shapes on their cellular uptake behaviors. This work should open a facile way to prepare various fluorescent inorganic nanoparticles with specific morphology for various biological applications.
研究领域:
材料科学
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生物医学工程
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细胞生物学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zhihui Zhong,Qinglei Cui,Tangsheng Guan,Hongyu Wen,Yu-xin Chen,Yu-Xin Ye,Jianqiao Xu,Gangfeng Ouyang
Background Aggregation-induced emission luminogens (AIEgens) exhibit efficient fluorescence in the aggregated state, making them promising candidates for bioimaging applications. However, their inherently hydrophobic structures often lead to uncontrollable aggregation, hindering their delivery to target sites for imaging. Researchers have developed strategies to enhance the water solubility of AIEgens through either structural modification or carrier-based encapsulation. However structural modification methods involve complicated synthesis and macrocycles often show low encapsulation efficiency toward AIEgens. Improving the water solubility of AIEgens for bioimaging requires simple yet effective strategies. Results We report a novel supramolecular encapsulation approach utilizing water-soluble metal–organic cages (MOCs) as host carriers for AIEgens. Encapsulation significantly prevented the aggregation of AIEgens, enabling their uniform dispersion in aqueous matrices. The AIEgens@MOC complex exhibited excellent structural stability across broad ranges of pH (5.0-9.0), temperature (10°C-80°C), and time (over 14 days) prior to bisulfite (HSO 3 - ) stimulation, confirming the stable host–guest interaction. Upon stimulation with HSO 3 - , the disassembly of MOC led to the release of AIEgens, which subsequently aggregated and exhibited significantly enhanced fluorescence within 60 s. The new AIEgens@MOC complex enabled the succeeded visualization of abnormal intracellular HSO 3 - fluctuations ( > 20 μM). Moreover, the low cytotoxicity of AIEgens@MOC facilitated its application as an intracellular imaging probe for HSO 3 - , with the capability of specific lysosomal localization. Significance We have developed a simple and effective strategy to improve the water solubility of AIEgens for bioimaging of important biomarkers in live cells. This method avoided complex chemical modifications, and enabled efficient detection of HSO 3 - in biological systems. By selecting MOCs with appropriate cavity sizes, this strategy is promising to accommodate various AIEgens in the future, offering a generalizable solution to facilitate their bioimaging applications.
研究领域:
超分子化学
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生物成像
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材料科学
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有机电子学
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细胞生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 18
·10440
·
IF 6.208
Yingxiang Zhai,Jiguo Zhang,Jian Li,Shouxin Liu,Zhijun Chen,Shujun Li
The potential for leakage of liquid electrolytes from magnesium (Mg) batteries represents a large hurdle to future application. Despite this, there are no efficient sensing technologies to detect the leakage of liquid electrolytes. Here, we developed a sensor using laccaic acid (L-AIEgen), a naturally occurring aggregation-induced emission luminogen (AIEgens) isolated from the beetle Laccifer lacca. L-AIEgen showed good selectivity and sensitivity for Mg2+, a universal component of electrolytes in Mg batteries. Using L-AIEgen, we then produced a smart film (L-AIE-F) that was able to sense leakage of electrolytes from Mg batteries. L-AIE-F showed a strong “turn-on” AIE-active fluorescence at the leakage point of electrolyte from model Mg batteries. To the best of our knowledge, this is the first time that AIE technology has been used to sense the leakage of electrolytes.Keywords:laccaic acid;aggregation-induced emission;smart film;detect of Mg2+
研究领域:
材料科学
·
化学
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DOI
PubMed
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 9
·2001750
·
IF 16.806
Ying Li,Fei Liu,Jiangjiang Zhang,Xiaoye Liu,Peihong Xiao,Haotian Bai,Shang Chen,Dong Wang,Simon H. P. Sung,Ryan T. K. Kwok,Jianzhong Shen,Kui Zhu,Ben Zhong Tang
Bacteria infected cells acting as “Trojan horses” not only protect bacteria from antibiotic therapies and immune clearance, but also increase the dissemination of pathogens from the initial sites of infection. Antibiotics are hard and insufficient to treat such hidden internalized bacteria, especially multidrug-resistant (MDR) bacteria. Herein, aggregation-induced emission luminogens (AIEgens) such as N , N -diphenyl-4-(7-(pyridin-4-yl) benzo [ c ] [1,2,5] thiadiazol-4-yl) aniline functionalized with 1-bromoethane (TBP-1) and (3-bromopropyl) trimethylammonium bromide (TBP-2) (TBPs) show potent broad-spectrum bactericidal activity against both extracellular and internalized Gram-positive pathogens. TBPs trigger reactive oxygen species (ROS)-mediated membrane damage to kill bacteria, regardless of light irradiation. TBPs effectively kill bacteria without the development of resistance. Additionally, such AIEgens activate mitochondria dependent autophagy to eliminate internalized bacteria in host cells. Compared to the routinely used vancomycin in clinic, TBPs demonstrate comparable efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in vivo. The studies suggest that AIEgens are promising new agents for the treatment of MDR bacteria associated infections.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Shunni Dong,Qiguang Zang,Zhao-Yu Ma,Meiqi Tang,Zhi-Kang Xu,Jingjing Nie,Binyang Du,Jing Zhi Sun,Ben Zhong Tang
The proposal of the aggregation-induced emission (AIE) effect shines a light on the practical application of luminescent materials. The AIE-active luminescence microgels (TPEC MGs) with photo-induced color-changing behavior were developed by integrating positively charged AIE luminogens (AIEgens) into the anionic network of microgels, where AIEgens of TPEC were obtained from the quaternization reaction between tetra-(4-pyridylphenyl)ethylene (TPE-4Py) and 7-(6-bromohexyloxy)-coumarin. The aqueous suspensions of TPEC MGs exhibit a significant AIE effect following the enhancement of quantum yield. In addition, further increase in fluorescence intensity and blueshift occur at elevated temperatures due to the collapse of microgels. The distinctive photochromic behavior of TPEC MGs was observed, which presents as the transition from orange-yellow to blue-green color under UV irradiation, which is different from TPEC in good organic solvents. The phenomenon of color changing can be ascribed to the competition between photodimerization of the coumarin part and photocyclization of TPE-4Py in TPEC. The photochromic TPEC MG aqueous suspensions can be conducted as aqueous microgel inks for information display, encryption, and dynamic anticounterfeiting.
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PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 12
·2202907
·
IF 10
Xinghua Yu,Ming Lyu,Xupei Ou,Wenquan Liu,Xing Yang,Xiaoxi Ma,Tianfu Zhang,Longnan Wang,Ying-Chuan Zhang,Sijie Chen,Ryan T. K. Kwok,Zheng Zheng,Hong-Liang Cui,Lintao Cai,Pengfei Zhang,Ben Zhong Tang
Aggregation-induced emission luminogens (AIEgens) are widely used as photosensitizers for image-guided photodynamic therapy (PDT). Due to the limited penetration depth of light in biological tissues, the treatments of deep-seated tumors by visible-light-sensitized aggregation-induced emission (AIE) photosensitizers are severely hampered. Microwave dynamic therapy attracts much attention because microwave irradiation can penetrate very deep tissues and sensitize the photosensitizers to generate reactive oxygen species (ROS). In this work, a mitochondrial-targeting AIEgen (DCPy) is integrated with living mitochondria to form a bioactive AIE nanohybrid. This nanohybrid can not only generate ROS under microwave irradiation to induce apoptosis of deep-seated cancer cells but also reprogram the metabolism pathway of cancer cells through retrieving oxidative phosphorylation (OXPHOS) instead of glycolysis to enhance the efficiency of microwave dynamic therapy. This work demonstrates an effective strategy to integrate synthetic AIEgens and natural living organelles, which would inspire more researchers to develop advanced bioactive nanohybrids for cancer synergistic therapy.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yunxia Wang,Min Ren,Ying Li,Feng Liu,Yu Wang,Zhijun Wang,Liheng Feng
Diseases caused by bacterial infections are increasingly threatening human health. As a major part of the microbial family, Gram-positive bacteria induce severe infections in hospitals and communities. Therefore, developing antibacterial materials that can recognize bacteria and specifically kill them is significant to cope with fatal bacterial infection. To this end, we designed and prepared a series of positively charged photosensitizers with an aggregation-induced emission feature and a type I reactive oxygen species (ROS) generation ability. Based on a molecular engineering strategy, the PS abbreviated to MTTTPy that owns a superior ROS generation ability and red emission in aggregation is obtained by adjusting bridging groups. Due to the unique molecular structure, MTTTPy can sensitively and specifically recognize and light up Gram-positive bacteria through electrostatic adsorption and void permeability. In addition, it can kill 95% of the recognized bacteria at a low concentration of 0.5 μM by generating oxygen-independent ROS under white light irradiation. Both in vitro and in vivo studies verify the sensitive and specific recognition and killing effect of MTTTPy toward Gram-positive bacteria. This work provides superior material-integrated diagnosis and treatment for Gram-positive bacteria-caused infectious diseases and shows potential for addressing bacterial resistance.
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PubMed
高影响因子
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