FIBERS AND POLYMERS
·
2021
·Vol. 22
·期 11
·2972-2978
·
IF 2.153
You Haining,Zhang Qiang,Yan Shuqin,You Renchuan
Chondroitin sulfate (CS) is a naturally derived bioactive macromolecule and has been used to improve the performance of silk fibroin (SF) biomaterials for soft tissue regeneration. However, it is still a challenge to use a chemical-free method to prepare water-insoluble SF/CS scaffolds. Here, a novel all-aqueous process was developed to prepare SF/CS scaffolds with controlled properties. Freezing-annealing treatment was used to induce silk I crystallization of SF to entrap CS macromolecules. It was found that the addition of CS did not prevent silk I crystallization of SF, even with a ratio as high as 10 % addition ratio. With increasing CS content, the water solubility of the scaffolds increased due to insufficient silk I crystals. The physicochemical properties of the scaffolds were evaluated, and the results showed that the water binding capacity and mechanical properties of the SF scaffolds can be effectively regulated by adding only less than 5 % CS content. This study provides a green strategy, including all-aqueous, low temperature, and without the use of toxic chemicals or solvents, to prepare bioactive SF/CS scaffolds for use in tissue engineering.
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高影响因子
ACS Biomaterials Science & Engineering
·
2026
·
IF 5.5
Yansong Wang,Yichong He,Yuxun Wu,Enze Zhao,Hanwei Huang,Zhengbin Yan,Hainan Shen,Fenbo Ma,ShuaiShuai Cao,Bin Tang
Background: Polyetheretherketone (PEEK) is a promising alternative to titanium alloy for dental implants due to its bone-mimicking elastic modulus, which mitigates stress shielding. However, its bioinert nature limits osseointegration. Methods: We developed a novel PEEK variant, PEEK-chondroitin sulfate zinc (PEEK-CSZn), by chemically grafting zinc and chondroitin sulfate onto the PEEK surface. Material properties were characterized using SEM, FTIR, EDS, and ICP-MS. Anti-inflammatory, osteogenic, and angiogenic effects were evaluated in vitro using MC3T3-E1, HUVEC, and RAW264.7 cells and in vivo using a rabbit femur bone defect model. Results: Characterization confirmed successful CSZn integration. In vitro, PEEK-CSZn at 500 μg/mL enhanced the MC3T3-E1 cell proliferation. Osteogenic markers (OCN and Osterix) were upregulated by around 2.3- and 1.8-fold, respectively, in MC3T3-E1 cells (p < 0.05). Inflammatory markers (IL-6 and IL-12a) in RAW264.7 cells decreased by 23% and 49%, respectively (p < 0.05), while angiogenic markers (VEGF and CD31) in HUVECs increased by 2.2- and 2.8-fold (p < 0.05). In vivo, Micro-CT analysis revealed PEEK-CSZn increased bone volume fraction (BV/TV) and BMD compared to unmodified PEEK at 8 weeks postimplantation (p < 0.05). Conclusions: PEEK-CSZn exhibits trifunctional bioactivities, including anti-inflammatory, osteogenic, and angiogenic, and thus significantly enhances osseointegration, making it a promising material for advanced dental implant applications.
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高影响因子
BIOPOLYMERS
·
2019
·Vol. 110
·期 12
·e23328
·
IF 1.845
Feng-Miao Zhang,Lei Zhou,Zheng-Nan Zhou,Cong Dai,Lei Fan,Chang-Hao Li,Cai-Rong Xiao,Cheng-Yun Ning,Yi Liu,Jian-Qiang Du,Guo-Xin Tan
Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation. Most of the current methods promote vascularization by adding specific growth factors, which are costly and easy to inactivate. In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties. The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA. Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel. The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs. Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis. This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties. Graphical
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Menghang Zu,Lijun Ma,Xueqing Zhang,Dengchao Xie,Yuejun Kang,Bo Xiao
Targeted delivery of chemotherapeutic drugs to tumors is a major challenge in colon cancer chemotherapy. To overcome this bottleneck, we loaded camptothecin (CPT) into polymeric nanoparticles (NPs), and further functionalized their surface with chondroitin sulfate (CS). The resulting CS-CPT-NPs had a desirable hydrodynamic diameter (289 nm), narrow particle size distribution (polydispersity index = 0.192) and neutral surface charge. Furthermore, in vitro experiments revealed that the surface functionalization of CS endowed NPs with the capacity of colon cancer-targeted drug delivery, and significantly improved the anti-colon cancer activities and pro-apoptosis effects against colon cancer cells. Strikingly, treatment of colon tumor-bearing mice with different NPs clearly indicated that CS-CPT-NPs showed much better therapeutic outcomes than non-targeted NPs and no systemic toxicity. Taken together, these results demonstrated the promising potential of CS-CPT-NP as an effective drug delivery system for colon cancer-targeted chemotherapy.
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RSC Advances
·
2015
·Vol. 5
·期 99
·81388-81394
·
IF 3.84
Shu-Jun Wang,Bei-Bei Wang,Feng-Wu Bai,Xiao-Jun Ma
Mass production of carbon dots (CDs) derived from chondroitin sulfate (CS) was developed by the facile hydrothermal approach for the first time. The CS derived CDs (CSCDs) possessed good dispersibility and water solubility, bright blue and green luminescence, and relative pH- and photo-stable properties. Moreover, the multicolor CSCDs could be efficiently uptaken by SAS cells and exhibited low cytotoxicity. Therefore, the responses of human oral squamous cell carcinoma SAS cells to CSCDs were further investigated by evaluating their proliferation and invasion. Compared to CS, CSCDs not only provided higher efficiency for proliferation of SAS cells, and up-regulated expression of matrix metalloproteinases to mimic extracellular matrix secretion, but also portrayed fluorescence for labeling SAS tumor cells. Hence, the multifunctional CSCDs are expected to have potential for biomedical applications.
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Biomaterials Science
·
2019
·Vol. 7
·期 6
·2308-2316
·
IF 5.251
Jingyi Yang,Shan Jiang,Yong Guan,Juan Deng,Shaofeng Lou,Dandan Feng,Deling Kong,Chen Li
Islet transplantation is one of the most promising therapeutic options that could restore euglycaemia in type 1 diabetic individuals. The currently implemented alginate microsphere islet encapsulation approach has led to positive outcomes in improving intraportal islet delivery in rodents. However, results obtained from human clinical trials remain disappointing. The less than satisfactory clinical outcome is mainly attributable to the increased size of the encapsulated islet and alginate-elicited host immune responses. In order to achieve islet encapsulation without significant alteration of the islet size, we designed and prepared a chondroitin sulfate (CS)-incorporated starPEG nanocoating (CS-PEG) that conjugates covalently to the pancreatic islet cell surface amine groups via pseudo-orthogonal chemistry for islet surface engineering. CS-PEG surface engineering incurred minimal alteration of the islet volume. Enhanced in situ revascularization, which is protective against extracellular matrix disruption, was also observed from CS-PEG islets in addition to robust islet viability and uncompromised insulin secretory ability. More importantly, CS-PEG surface engineering reduced blood coagulation while facilitating islet cell survival under pro-inflammatory conditions. Given that host immune rejection and an instant blood-mediated inflammatory reaction (IBMIR) are the primary factors detrimental to islet engraftment and survival, often resulting in rapid cell loss and graft failure, CS-PEG surface engineering provides an “easy-to-adopt” approach for cell surface engineering that could potentially improve the clinical efficacy of islet transplantation and other types of cell therapies.
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FIBERS AND POLYMERS
·
2023
·Vol. 24
·期 3
·975-984
·
IF 2.5
Luo Honglin,Yin Cheng,Zhong Bingbing,Li Wei,Yang Zhiwei,Zhu Hongmin,Zhan Yuliang,Cai Xinyong,Ren Kaijing,Zhang Quanchao,Wan Yizao
Lacking large pores and insufficient bioactivity are two critical disadvantages of bacterial cellulose (BC). In this study, a new type of coating, chondroitin sulfate (CS) modified with gelatin (Gel), was selected to modify porous bacterial cellulose (PBC) for improved biocompatibility. The PBC was fabricated by adding Gel microspheres in the culture medium of BC, and the PBC-CS/Gel scaffolds were prepared by immersing PBC in the CS solutions followed by Gel modification. The morphology, chemical structure, physicochemical properties, and in vitro cell behavior of the PBC-CS/Gel scaffolds were characterized. The mechanical test shows that the tensile strength and Young's modulus of the PBC scaffold were increased with the introduction of CS/Gel coatings. The in vitro cell studies show that the CS/Gel coatings significantly promote cell proliferation, adhesion, differentiation, and ingrowth into scaffolds. The PBC-CS/Gel scaffolds are expected to be used in tissue engineering.
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高影响因子
DENTAL MATERIALS
·
2026
·
IF 6.3
Changyu Shao,Tong Shi,Dayu Wang,Chang Liu,Zhenhang Tang,Yuanyu Zhou,Haihua Pan,Zhijian Xie,Ruikang Tang,Zhuo Chen
Chondroitin sulfate mediates the amorphous calcium phosphate ordered assembly. • Chondroitin sulfate directs the oriented crystallization of the hydroxyapatite. • Biomimetic restores the enamel hierarchical structure and mechanical properties. • Hydrogel mineralization system demonstrates significant efficacy for enamel repair. Objectives This study aims to develop a biomimetic mineralization strategy using chondroitin sulfate (CS) to reconstruct the intricate hierarchical structure of enamel and restore its mechanical properties. Methods Demineralized enamel was remineralized in a CS-containing artificial saliva (AS). The crystallization of hydroxyapatite (HAP) in AS was investigated using transmission electron microscopy (TEM) and atomic force microscopy (AFM)-infrared spectroscopy. Enamel repair efficacy was systematically evaluated via scanning electron microscopy (SEM) and AFM to characterize the microstructure and repair thickness, while nanoindentation was employed to quantify the mechanical properties. In addition, acid resistance was evaluated using a 50 mM HCl solution. Furthermore, an AS-hydrogel mineralization system was developed to evaluate its efficacy in restoring demineralized enamel under biologically relevant conditions. Results The results demonstrate that CS-modified ACP successfully assembles into chain-like precursors via anisotropic forces, which simultaneously regulate the oriented crystallization of HAP. This strategy resulted in a newly formed mineralized layer that effectively reconstructs the hierarchical architecture and recovers mechanical properties, including hardness, elastic modulus, and coefficient of friction, to near-native levels. In addition, the repaired enamel exhibits an acid resistance level comparable to that of native enamel. Significance This study establishes a new, mechanism-driven strategy for enamel remineralisation that is mediated by CS, and a corresponding hydrogel mineralization system, which can be translated into practice, offering a promising approach to future clinical enamel restoration. Download: Download high-res image (411KB) Download: Download full-size image
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Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Ma Fenbo,Li Sijing,L.I. Ruiz-Ortega,Zhang Yuanjun,Xu Lei,Wang Kui,Lin Lijun,Tang Bin
Repairing bone defects remains challenging in orthopedics. Here, strontium (Sr) alginate hydrogels containing chondroitin sulfate (CS) were fabricated for enhancing bone defects repair. The effects of CS incorporation ratio on the morphology, structure, thermal stability, water uptake and mechanical performance of Sr-CS/alginate hydrogels were also evaluated. Increasing CS incorporation ratio, Sr-CS/alginate hydrogels exhibit decreasing mechanical properties and lower water retention capacity. In vitro results suggest that Sr-CS/alginate hydrogels with higher CS ratio facilitate the proliferation of osteoblasts. Additionally, the osteogenic genes expressions were investigated by real-time quantitative polymerase chain reaction (RT-qPCR). The results reveal that Sr-CS/alginate hydrogels should have positive effects on modulating the osteogenic factors. Moreover, by employing repair femoral cylindrical defects rabbit model, the efficiency of as-fabricated Sr-CS/alginate hydrogels in bone regeneration was evaluated. The animal study suggests that Sr-CS/alginate hydrogel could significantly facilitate bone defects repair and therefore should potentially be useful for osteochondral tissue engineering.
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Polymers
·
2023
·Vol. 15
·期 2
·271
·
IF 5
Zuwu Tang,Meiqiong Yu,Ajoy Kanti Mondal,Xinxing Lin
In this paper, porous scaffolds based on composite hydrogels were fabricated using polydopamine (PDA), chondroitin sulfate (CS), and polyvinyl alcohol (PVA) via the freezing/thawing method. Different characteristics of the prepared composite hydrogels, including the pore sizes, compression strength, lap shear strength, mass loss, and cytocompatibility were investigated. Scanning electron microscope images (SEM) displayed the hydrogel pore sizes, ranging from 20 to 100 μm. The composite hydrogel exhibited excellent porosity of 95.1%, compression strength of 5.2 MPa, lap shear strength of 21 kPa on porcine skin, and mass loss of 16.0%. In addition, the composite hydrogel possessed good relative cell activity of 97%. The PDA/CS/PVA hydrogel is cytocompatible as a starting point, and it can be further investigated in tissue engineering.Keywords:composite hydrogel;cytocompatibility;porosity;porous scaffolds
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APPLIED SURFACE SCIENCE
·
2016
·
IF 3.15
Huihui Yuan,Jing Xue,Bin Qian,Huaying Chen,Yonggang Zhu,Minbo Lan
An antifouling polyurethane film modified by chondroitin sulfate (PU-CS) was prepared by chemical grafting with N-Boc-1,3-propanediamine as a spacer. The different mass fraction of N-Boc-1,3-propanediamine was investigated to obtain PU-CS films with different CS grafting density. The surface properties of PU-CS films were comprehensively characterized. Proteins adsorption and glycosaminoglycans adhesion on films were evaluated. Moreover, inorganic salt deposition on film with highest CS grafting density (3.70 μg/cm 2 ) was briefly investigated. The results showed that the increase of CS grafting density improved not only the hydrophilicity but the antifouling performance of films. The best antifouling film reduced the adsorption of fibrinogen (BFG), human serum albumin (HSA) and lysozyme (LYS) by 81.4%, 95.0% and 76.5%, respectively, and the adhesion of chondroitin (CS), heparin (HP) and hyaluronic acid (HA) by 70.6%, 87.4% and 81.3%, respectively. In addition, the co-adsorption of proteins and glycosaminoglycans reduced up to 86.9% and 75.5%, respectively. Changes in inorganic salt deposition after co-adsorption of proteins and glycosaminoglycans on PU-CS(3) suggested that the proteins promoted the inorganic salt deposition, while glycosaminoglycans inhibited the crystal growth. The negatively charged polysaccharides might promote the generation of smaller crystals which could be conducive to provide theoretical and practical guide to develop novel urinary stents with significant anti-encrustation properties.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Bin Chu,Yibin Huang,Yifan Chen,Song Wang,Xiaoli Li
Developing durable anticoagulant coatings for cardiovascular implants remains a major research challenge. Heparin, the current gold-standard anticoagulant, faces challenges due to rapid elution from coated surfaces. This article reports a new heparin-alternative anticoagulant coating system engineered through rational design of a dual-functional chondroitin sulfate derivative (methacrylated/aldehyde-modified chondroitin sulfate, CSMAO) and sulfonated chitosan (SCS). The hierarchical architecture was constructed via sequential layer-by-layer (LbL) assembly on polydopamine (PDA)-functionalized 316 L stainless steel substrates. The material characterization methods included Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), and X-ray photoelectron spectroscopy (XPS). The grafting rate of glycidyl methacrylate was 20.05 %, with an aldehyde substitution degree of 48.61 %, and the sulfonation degree of SCS reached 46.96 %. The composite coating exhibited excellent biocompatibility and anticoagulant properties. Coagulation profiling indicated comparable extrinsic pathway regulation to that of heparin controls along with superior intrinsic pathway inhibition (P < 0.01). This rationally designed coating system shows translational promise for improving the hemocompatibility of cardiovascular implants.
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