FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Yuzhu Zhao,Huawei Gu,Rodrigo Ledesma-Amaro,Ling He,Shuwen Liu,Kan Shi
β-Glucosidase (BGL) plays a key role in wine aroma enhancement by hydrolyzing glycosidic precursors; however, its application is limited by instability under harsh winemaking conditions. To address this, we developed an integrated biocatalytic system by immobilizing an acid-tolerant BGL onto functionalized magnetic oak chips (NFOak) via oriented Ni 2+ -histidine coordination. Comprehensive characterization confirmed the fabrication of a porous, magnetic cellulose scaffold. The immobilized BGL exhibited significantly enhanced stability against winemaking stresses (low pH, high ethanol) compared to the free enzyme. Furthermore, magnetic actuation of the biocomposite improved its hydrolytic performance, likely attributed to enhanced mass transfer. When applied to Chardonnay and Marselan wines, the magnetically actuated system effectively hydrolyzed glycosidic precursors, markedly increasing the concentrations of key volatile compounds-such as linalool and ethyl benzoate associated with fruity and floral aroma profiles. The treatment also induced subtle modulations in wine color and phenolic composition. Safety assessments confirmed no nickel leaching and oenologically acceptable iron levels. This work provides a sustainable and efficient strategy for controllable aroma modulation, combining the oenological benefits of a natural oak carrier with the operational controllability of magnetic actuation.
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Journal of Materials Chemistry A
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2026
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IF 9.5
Guangmeng Chen,Ziqiang Hu,Yuan Liu,Li Jia,Weiwei Zhao,Xiaoqing Liu
Non-enzymatic electrochemical glucose sensors (NEGS) offer advantages over enzymatic counterparts, but conventional supporting scaffolds suffer from poor conductivity, limited surface area, and binder dependence. Herein, we present a strategy to fabricate high-performance, binder-free NEGS based on a hierarchical laser-induced graphene/carbon fiber framework (LW-CFF-B) functionalized with NiO nanoparticles. The 3D porous LW-CFF scaffold is engineered by in-situ carbon fiber felt (CFF) enhanced LIG using 1,4-phenylenediboronic acid (PA) modified liquid benzoxazine (PGE-a) as the precursor. PA serves a dual role in enhancing carbon yield and improving electrical conductivity (sheet resistance reduced from 2.54 to 1.23 Ω/sq). Moreover, PA promotes the formation of a hierarchical porous carbon architecture, which is beneficial for facilitating electrochemical performances of the obtained carbon product. Subsequent laser assisted deposition of NiO nano-layer on LW-CFF-B scaffold yields the LW-CFF-B-Ni electrode. This optimized architecture combines mechanical integrity with exceptional electrocatalytic properties, leading to outstanding glucose sensing performance: ultrafast response (1.2 s), ultrahigh sensitivity (5758 μA mM⁻¹ cm⁻²), a low detection limit (0.16 μM), and excellent selectivity against common interferents. The developed sensor demonstrates appreciable recoveries (91.1% to 107.3%) regarding glucose concentration in beverages. These outstanding analytical results demonstrate significant potential for this sensor in precise glucose monitoring.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.7
Gongde Lan,Yuchen Liu,Hongyuan Mao,Han Yu,Weicheng Huang,Pengxiang Chen,Xin Chen,Qingxu Song,Yufeng Cheng,Yuxia Luan
Boron neutron capture therapy (BNCT) is a promising strategy for selective tumor eradication. However, the clinical application of the boron delivery agent p ‑boronophenylalanine (BPA) is limited by challenges such as poor solubility, suboptimal tumor accumulation, and lack of effective imaging capabilities. Here, we present a metal polyphenol-based boron nanodrug (EB@Gd NPs), designed to address these challenges and enhance the efficacy of BNCT. Self-assembled from epigallocatechin-3-gallate (EGCG), BPA, and gadolinium ions (Gd 3+ ), the EB@Gd NPs exhibit significantly improved tumor targeting and enable real-time magnetic resonance imaging (MRI). These nanoparticles show enhanced tumor accumulation, with MRI providing dynamic insights into boron distribution, thus aiding in determining optimal timing for neutron irradiation. Furthermore, EGCG potentiates therapeutic effect of BNCT by inhibiting DNA repair and promoting efficient DNA double-strand breaks. Therefore, the EB@Gd NPs demonstrate potent tumor growth inhibition in BNCT, holding great potential for improving precision and efficacy of BNCT in cancer therapy.
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CHEMICAL ENGINEERING SCIENCE
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2026
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IF 4.3
Jiafang Wang,Bingchen Guo,Boyu Zhang,Yexin Dai,Yiran Cheng,Shilong Chang,Zhenhua Wu,Shujing Zhang,Dong Yang,Jiafu Shi,Zhongyi Jiang
Enzyme catalysis is widely used in industrial bioprocesses, but free enzymes often suffer from low stability, poor reusability, and limited mass transfer. Capsule encapsulation improves stability by providing a cell-mimetic confined microenvironment, while limited shell permeability often restricts mass transfer, reducing catalytic activity. In this work, the immobilized enzyme system (MDH@OSN-RCC2) was prepared by incorporating the porous organic cage RCC2 into an organosilica network (OSN) for the encapsulation of mannitol dehydrogenase (MDH). RCC2 integration adjusted the capsule’s pore structure. At 3 g L −1 RCC2, the specific surface area and total pore volume increased by 3.48-fold and 3.31-fold, the diffusion rate of fructose increased 6.84-fold, and the catalytic efficiency ( k cat / K m ) was 2.05-fold higher than MDH@OSN. The system retained 71.8 % of initial activity after 11 reuse cycles and performed stably over a broad temperature and pH range. This strategy was also applied for whole-cell immobilization that exhibited excellent operational stability and environmental tolerance. This study offers a design principle for durable biocatalysts in industrial and green biomanufacturing applications.
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TALANTA
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2026
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IF 6.7
Xiao Ma,Junjie Wang,Xin Chen,Jingcheng Li,Jiaqi Xue,Yufan Hou,Shihao Zhao,Jingxiang Zhao,Xiaomi Meng,Huajun Chen,Fang Wang,Fangya Guo,Jian Sun,Dan Zhao
Oxidase-like nanozymes use dissolved O 2 as the terminal electron acceptor and do not require exogenous H 2 O 2 , offering methodological advantages; however, conventional “one-pot carbonization” often causes concurrent carbonization and active-site formation, making the coordination environment difficult to control. Here, a sequential “pre-coordinate-polymerize-pyrolyze” site-engineering route was developed. On carbon black, OPD-Fe 3+ pre-coordination and in situ oxidative polymerization were carried out, followed by thermal treatment and purification, by which Fe–N moieties were immobilized within the shell region of the material to afford highly dispersed Fe–N x moieties predominantly featuring Fe–N 4 -like coordination (Fe–N 4 @CB d ). Multiscale characterization (XRD/XPS/XANES/EXAFS) consistently showed that iron resided predominantly in a first coordination sphere dominated by Fe–N (coordination number CN ≈ 3.7; R ≈ 2.01 Å), with no resolvable Fe–Fe scattering and only minor, non-dominant Fe-containing phases. ROS-trapping and time-dependent EPR measurements further confirmed continuous O 2 activation with the generation of O 2 • - , •OH, and 1 O 2 in the Fe–N 4 @CB d /O 2 system. DFT calculations additionally supported Fe–N 4 -like Fe–N x sites as the most plausible dominant active structures for O 2 activation. Under a unified TMB/O 2 system, steady-state enzyme kinetics exhibited outstanding performance, with V max = 351 nmol L −1 s −1 and K m = 0.56 mM, which are competitive among reports using the same substrate/readout. An H 2 O 2 -free colorimetric platform for alkaline phosphatase (ALP) was constructed on this material, achieving a low limit of detection and good linearity; acceptable accuracy and precision were verified by standard additions in diluted serum. This sequential strategy predefines the Fe–N neighborhood and topology at the precursor stage, reduces uncertainty in site formation, and avoids metal-organic framework (MOF)/hard-template/ionic-liquid multi-step procedures, thereby providing a strong basis for reproducible Fe–N 4 -like/Fe–N x oxidase-like nanozyme preparation and streamlined, robust bioanalysis.
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BIORESOURCE TECHNOLOGY
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2026
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IF 8.2
Fangyuan Zhou,Kezhen Jia,Hongke Zhang,Wanbin Zhu,Hongliang Wang
Develop AlCl 3 -assisted mechanochemical strategy enabling efficient one-pot conversion of recalcitrant cellulose to HMF. • Synergistic interplay between Lewis acidity and mechanical stress disrupts H-bonding networks and lowers crystallinity, markedly enhancing the chemical reactivity of cellulose. • Optimization of the Brønsted/Lewis acid balance by controlled HCl addition achieves a high HMF yield of 62.8% from mechanochemically pretreated cellulose. • This green and scalable approach can be broadly extended to diverse lignocellulosic biomass wastes, including waste paper, corncob residue, bamboo powder, and corn straw, to efficiently produce HMF. 5-Hydroxymethylfurfural (HMF) is a pivotal bio-based platform chemical, yet its direct production from inexpensive and abundant cellulose remains challenging due to the intrinsic recalcitrance of cellulose and the competing degradation of intermediates and products under acidic conditions. Herein, we report an AlCl 3 -assisted mechanochemical strategy that couples catalytic Lewis acidity with ball-milling mechanical force to activate cellulosic feedstocks, enabling their efficient one-pot conversion to HMF. Detailed structural characterizations demonstrate that the synergistic interplay between AlCl 3 and mechanical stress disrupts the extensive hydrogen-bonding network and partially cleaves β-1,4-glycosidic linkages, leading to pronounced reductions in cellulose crystallinity and degree of polymerization and, consequently, markedly enhanced chemical reactivity. The resulting mechanochemically pretreated cellulose (BMM-AlCl 3 ) was directly converted into HMF without additional catalysts during the reaction step, as the Al species introduced during pretreatment served as the catalytic sites, affording a yield of up to 46.8%. By further optimizing the Brønsted/Lewis acid balance through controlled HCl addition, the HMF yield increases to 62.8%. Importantly, this pretreatment strategy is broadly applicable to diverse lignocellulosic biomass resources, including waste paper pulp, corncob residue, bamboo powder, and corn straw, consistently delivering high HMF yields. This work establishes an efficient and potentially scalable mechanochemical route for the valorization of cellulose and lignocellulosic biomass into HMF. Download: Download high-res image (185KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jing Chen,Wenli Xue,Yaqin Shi,Wenjing Liu,Rongfang Ma
Catechin is a natural flavonoid with antioxidant, anticarcinogenic and anti-inflammatory properties. They can be used to treat or prevent human health problems. However, excessive intake of catechin can also be harmful to the human body. It is crucial to find a method for quantitative detection of catechin. The carboxyl-functionalized multi-walled carbon nanotubes were intercalated into the MXene layers to form a composite material with excellent electrical conductivity, and then MOF-808 with adsorption sites was further loaded on MXene/MWCNTs-COOH to synthesize MXene/MWCNTs-COOH/MOF-808 with excellent catalytic properties. Based on this, an electrochemical sensor was constructed for the detection of catechin. The CA sensor constructed in this work exhibited excellent analytical performance, good linear range (0.5–21.53 μM) and low detection limit (0.05 μM). It was also used for the detection of CA in green tea and beverages, which exhibited good recoveries (99.46–99.71 % and 96.46 % ~ 101.33 %) and the relative standard deviations (1.33–2.55 % and 0.07 % ~ 1.57 %).
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ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Fuhui Luo,Fenfen Zeng,Zhongqiao Zhao,Xu Ren,Yong He,Xia Yang,Fengxiang Zhang,Hanqing Pang,Wei Shi
The development of efficient and green extraction methods to meet the needs of the food and pharmaceutical industries for natural products has received widespread attention. In this work, a novel two-step extraction process for acid-electrolyzed oxidizing water (AOEW) pretreatment with deep eutectic solvents (DESs) (EWDES) was designed. Screening of 25 ultrasound-assisted DESs and EWDESs revealed that the extraction rate of EWDES (chlorine chloride-acetic acid) (ChCl-AA) was significantly higher than that of UADES (ChCl-AA) and traditional solvents. Back-propagation-genetic algorithm proved superior to response surface methodology in robustness and predictive intelligence. The mechanism analysis demonstrated that the addition of AOEW significantly enhanced the destruction of the cell wall structure, facilitating the entry of DESs into cells and promoting the formation of hydrogen bonds with components, which aid in the delivery of active ingredients. Notably, the addition of AOEW further promotes the formation of hydrogen bonds between DESs and the active ingredients. The EWDES system demonstrates multifunctional capabilities in enhancing the natural product extraction efficiency through synergistic mechanisms. Furthermore, the green evaluation tools validated the environmental sustainability of EWDES.
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Haihui Ren,Wen Xiao,Jiameng Han,Qiaorong Liu,Zunjian Zhang,Yuan Tian
Background Tracking changes in plasma concentrations of monosaccharides and sugar alcohols related to glycometabolism, which plays a role as the main source of energy, is of great significance for the treatment of metabolic diseases. However, analysis of monosaccharides and sugar alcohols has always been a difficult task due to the high polarity of the polyhydroxy structure and the presence of a large number of isomers. So far, no suitable method has been developed for simultaneous determination of monosaccharides and sugar alcohols in beagle plasma. Results This work performed an innovative gas chromatography-mass spectrometry method for the simultaneous chromatographic separation and absolute quantification of 11 monosaccharides and sugar alcohols associated with glycometabolism. After derivatization, monosaccharides converted to be oxime acetylated derivatives and sugar alcohols transformed into acetylated derivatives. All analytes achieved good separation after chromatographic conditions were optimized, especially the selection of mid-polarity capillary column(6% cyanopropyl phenyl and 94% dimethyl polysiloxane). The improvement of derivatization efficiency through single factor and response surface optimization ensured a high sensitivity, with LOQ of each analyte in the range of 0.2-50 ng/mL. The established method was fully validated in terms of linearity and range, sensitivity, accuracy and precision, recovery, matrix effect, stability, dilution integrity and proved to be reliable, which was applied to the comparative study on hypoglycemic effect of two antidiabetic agents with different pharmacological mechanisms, dorzagliatin and metformin, as well as possible metabolic pathways affected by them. Significance This method solves the problems of multiple peaks after derivatization and frequent co-elution of analytes in previous analytical methods, significantly enhances derivatization efficiency of ketoses, which provides a promising approach for simultaneous analysis of aldose, ketose, and sugar alcohol. It offers new insights into a more comprehensive research on glycometabolism rather than merely focusing on glucose.
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FOOD CHEMISTRY
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2026
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IF 10.4
Weiwei Wu,Yanming Tuo,Zhihui Wang,Na Chen,Yuxue Jiang,Chuanjiu Wu,Yongquan Xu,Wen Zeng,Weijiang Sun
Tea flowers and seeds, non-leaf organs of Camellia sinensis , are often discarded, leading to inefficient resource utilization. This study evaluated the potential of tea flowers and seeds by preparing white teas from fresh leaves (WT), flowers (WTF), and seeds (WTS) of Camellia sinensis cv. Fuding Dabaicha. Sensory evaluation, targeted metabolomics, and molecular simulations were used to investigate taste components and their impact on pleasantness. WTF exhibited the highest pleasantness score (6.60), followed by WTS (6.36) and WT (6.10). Four sugars— d -fructose, glucose, D-galactose, and D-xylose—synergistically enhanced pleasantness, with a dose-dependent increase, especially d -fructose. Molecular simulations showed these sugars formed a stable binding network with the CHRM1 receptor (−60.04 kcal/mol), with d -fructose as the key contributor. Alanine scanning identified ARG123 as a critical binding residue. This study provides support for the high-value utilization of tea flowers and seeds and offers insights into enhancing the pleasantness of tea beverages.
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LANGMUIR
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2026
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IF 4.4
Yun Zhang,Wei Wei,Yiwen Guo,Jinyu Chai,Lu Liu,Jinhua Zhu
The conventional synthesis of superparamagnetic Fe3O4 superparamagnetic nanoparticles (Fe3O4SNPs) for biomedical applications often suffers from poor aqueous dispersibility, complex multistep processes, and potential toxicity from organic solvents or surfactants. Achieving precise control over both particle size and magnetic field-driven aggregation state in a biocompatible manner remains a significant challenge. A facile one-pot hydrothermal synthesis strategy was developed using a polysaccharide-iron complex as the precursor to prepare nano-Fe3O4 with excellent water dispersibility. With this method, we achieved precise regulation of Fe3O4 particle size (3.2–12.5 nm) and magnetic field-driven aggregation behavior by optimizing reaction conditions (such as sugar content, NaOH concentration, and reaction time), making them suitable for different biomedical scenarios such as the separation of active ingredients, in vivo targeted delivery, or MRI contrast agent. The solution of synthesized Fe3O4SNPs remained stable at room temperature for up to six months without any precipitation and did not exhibit significant toxicity at different particle sizes. In vitro MRI performance evaluation revealed that Fe3O4SNPs with a particle size of 8.1 nm exhibited a high transverse relaxation rate (r2 = 189.8 mM–1 s–1) and a prominent r2/r1 ratio of 172.5, demonstrating potential as a T2 contrast agent; nanoparticles with a particle size of 4.0 nm displayed an r1 value approximately 1.6 times that of Gd-DTPA contrast agents, showing application potential as T1 contrast agents. This green synthesis method only uses water and polysaccharide iron complexes, thus providing a technical reference for the production of medical magnetic nanomaterials.
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INDUSTRIAL CROPS AND PRODUCTS
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2026
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IF 6.4
Penghua Shu,Wenhao Zou,Yu Meng,Jiaqi He,Tingting Guo,Xinfeng Fan,Di Hu,Hao Yin,Shuxian Fan,Na Wang,Simin Liu,Lu Lv,Si Chen,Xialan Wei,Jihong Huang
Astragali complanati Semen (ACS) is frequently used as Chinese medicines and functional foods. Complanatoside A (CA), a key bioactive phytochemical isolated from ACS, exhibits broad-spectrum pharmacological properties. This investigation systematically formulated sixteen natural deep eutectic solvent (NADES) systems by combining three hydrogen-bond acceptors with eleven hydrogen-bond donors at varying molar ratios. These NADES formulations were subsequently employed in tandem with ultrasonic-assisted extraction to isolate CA. Optimization studies identified the following parameters as most effective for extraction using the L -proline/levulinic acid-based NADES (molar ratio 1:2): 60℃, 30 min, 40 % water content, liquid-solid ratio 25 mL/g, achieving an extraction yield of 1.57 %. The adsorption and desorption conditions were optimized employing a macroporous resin for the recovery of CA, while the reusability of the NADES solvent was thoroughly evaluated throughout the extraction process. The CA extract exhibited significant tyrosinase and α-glucosidase inhibitory activity. These findings establish ultrasound-assisted NADES extraction as a sustainable methodology for efficient extraction of CA from ACS, providing valuable insights for eco-friendly extraction and pharmacological exploitation of plant-derived bioactives.
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危险品化学品经营许可证（带存储）