TALANTA
·
2026
·
IF 6.7
Zifeng Wang,Cong Dai
The dual-channel fluorescent sensing of hydroxyl radicals (·OH) is of great importance for environment and life. Organic cage for fluorescence sensing suffers from short excitation/emission wavelength and poor water solubility, while the application of carbon quantum dots is also hindered by the time-consuming preparation in high pressure and temperature conditions. Herein, we report a red emitted carbon dots derived from cationic organic cage for dual-channel fluorescent sensing of ·OH. The abundant flexible methylene within the cationic organic cage leads to remarkable collapse of intrinsic cavity. The ring-opening of imidazole structure occurs via redox reaction at acidic condition, resulting in the simple formation of carbon dots with the emission at 640 nm. Under acidic conditions, the ·OH generated via Fenton reaction exhibits strong affinity toward the prepared carbon dots. The mild oxidation at pH 1 significantly enhances the fluorescence, whereas the severe oxidative degradation at pH 6 leads to obvious fluorescence quenching. Based on the mutual verification of dual signals, a dual-channel fluorescent sensing method is established for ·OH in environmental samples and biological fluids. The sensing at pH 6 and 1 respectively achieves the linearity range of 1-50 and 5-50 μM, the limit of detection of 7.3 and 11.8 nM, and the relative standard deviation of 0.76% and 0.83% for 10 replicate analysis. The proposed method gives the recoveries of spiked ·OH ranged from 97.3% to 103.4% in real samples, demonstrating excellent accuracy and reliability. This study underscores the promising application potential of organic cage and carbon dots in fluorescent sensing. Furthermore, combined with Fenton reaction, the proposed dual-channel sensing platform can also be utilized to the detection of hazardous substances or biomarkers capable of H 2 O 2 generation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2026
·
IF 5.2
Lu Sun,Jie Wei,Qing Hu,Xiuquan Xu,Jing Wu,Yaoyao Cao,Yao Zhang,Haoyan Wu
A Fe/V-CN nanozyme with synergistically enhanced POD-like activity was rationally designed. • A sensitive cascade colorimetric platform for detecting gastric cancer-related D-amino acids was successfully constructed. • The assay supports reliable quantification of D-amino acids in complex human saliva matrices. The development of a practical and reliable cascade colorimetric method for detecting D-amino acids (DAAs) provides a promising tool for the prevention and preliminary diagnosis of gastric cancer. Herein, a bimetallic Fe V supported on graphitic carbon nitride (Fe/V-CN) nanozyme was rationally designed and synthesized via a simple annealing strategy. The atomic dispersion of Fe and V species, along with their coexisting multivalent states (Fe 2+ / 3+ , V 4+ / 5+ ) synergistically boosted the intrinsic peroxidase-like (POD) activity to Fe/V-CN, which were characterized by a high substrate affinity ( K m of 0.078 and 0.083 mM for TMB and H 2 O 2 ) and an accelerated catalytic turnover ( V max of 4.94 × 10 −7 and 7.76 × 10 −7 M·s −1 , respectively). Leveraging this improved activity, a cascade colorimetric sensing system was developed by integrating Fe/V-CN with D-amino acid oxidase (DAAO) for the specific detection of DAAs. The platform exhibited favorable analytical performance for D-proline (D-Pro) and D-alanine (D-Ala), offering wide linear ranges of 0–200 μM and 0–160 μM and low detection limits of 0.93 μM and 1.45 μM, respectively, combined with acceptable selectivity against common interferents. For real-sample analysis, the platform was successfully implemented in determine DAAs in human saliva, delivering satisfactory recoveries (95.8–105.7%) and good precision (RSD < 3.5%). This work presented a synergistic Fe/V bimetallic nanozyme and a practical sensing strategy, demonstrating the feasibility of DAAs detection in complex biological matrices. Download: Download high-res image (70KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Simin Zhang,Xiangfeng Chen,Yaping Shi,Xiangyu Zhu,Zongwei Cai
As bioactive compounds, chiral amino acids (AAs) have been demonstrated to play pivotal roles in different physiological processes, thereby influencing human health. The assessment of the relative enantiomeric ratio through analytical methods is essential for indicating the abnormal status of disease progression, which drives the development of effective methods for resolving and identifying enantiomers. This work presents a robust strategy for the ultrahigh-resolution analysis of chiral AAs using MALDI-TIMS-MS. By employing a chiral derivatization process using S-NIFE and stabilizing the soft chain of the derivatization reagent with bimetal ion addition, the separation of 19 pairs of proteinogenic AAs was successfully achieved. This method not only demonstrates outstanding separation capability but also proves its efficiency in determining enantiomeric ratios, extending its applicability to real sample analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Mengmeng Ji,Jinwei Li,Liuping Fan,Liang Wang,Shengquan Huang
Pseudomonas aeruginosa , a significant opportunistic pathogen, poses a serious threat to food safety due to its antibiotic resistance and strong biofilm-forming ability. In this study, quercetagetin (Que) was identified as a novel quorum sensing (QS) inhibitor. Reporter strain assays, molecular docking, and dynamic simulation indicated that Que acts as an antagonist of the QS receptors. Que attenuated QS-regulated virulence phenotypes in a dose-dependent manner but showed insufficient antibiofilm activity ( p >0.05) at sub-minimum inhibitory concentration (sub-MIC) levels. In contrast, the combination of 0.5 mmol/L Que with 20 mmol/L D-leucine or D-tryptophan exhibited additive effects on planktonic cells, reducing its survival by 2.19–3.97 log compared to the control. For biofilm cells, the combined treatment potentiated the reduction in cell density by 0.48–1.42 log. Notably, these effects were strain-dependent but were equal to or outperformed those of the ε-polylysine (ε-PL) control. Mechanistically, this enhancement was driven by the disruption of biofilm matrix and cell membrane integrity. This was evidenced by the greater suppression of Acyl-homoserine lactones (AHLs) synthesis and virulence phenotypes, alongside substantial reductions in biofilm biomass and roughness and increased in membrane permeability compared to Que. Furthermore, the combination resulted in repression of key enzymes activities (Na + -K + -ATPase and succinate dehydrogenase) by 29%–38%. These changes indicated a promising strategy to control P. aeruginosa and enhance food safety.
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Huimin Wang,Guangxin Wang,Ruoxi Liu,Jin Yong Lee,Shufeng Liu,Yingqi Tang,Jing Yu
The development of highly efficient chiral recognition systems by simple and cost-effective means is critical to advance the precise application of chiral molecules. Herein, a novel electrochemiluminescence (ECL) enantioselective recognition platform was constructed based on l-histidine-functionalized zeolitic imidazolate frameworks (l-His-ZIF8). Detailed characterization verified the successful preparation of the chiral l-His-ZIF8 material. It was then applied to the chiral discrimination of six amino acids: cysteine (Cys), tryptophan (Trp), phenylalanine (Phe), glutamic acid (Glu), histidine (His), and proline (Pro). Among them, the system employing Ru(bpy)32+ as the luminophore demonstrated the most efficient chiral recognition of d-Cys over l-Cys, as evidenced by a distinct ECL signal ratio (ECLd-Cys/ECLl-Cys) of 3.3 at 1 mM. A detection limit of approximately 0.193 mM was achieved for Cys enantiomers. Conversely, an inverse chiral recognition was observed for the tryptophan (Trp)/sodium tetraphenylborate (TPB) system, resulting in a stronger ECL response for l-Trp over that for d-Trp (ECLl-Trp/ECLd-Trp = 2.4 at 1 mM). Theoretical calculations indicate that the excellent ECL enantioselectivity stems from the preferential binding of the “host” l-His-ZIF8 with specific “guest” enantiomers (d-Cys or l-Trp) via stronger intermolecular interactions. This preferential binding leads to the formation of more stable diastereoisomers that facilitate electrooxidation of more “guests” to generate a stronger luminescent signal than its isomer. This study establishes a novel strategy for electrochemiluminescent enantiomer discrimination using chiral site-engineered metal–organic frameworks (MOFs) and opens new avenues for the future of chiral sensing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysis Science & Technology
·
2023
·Vol. 13
·期 13
·3953-3962
·
IF 5
Xuexia Xu,Qin Yang,Lanteng Wang,Jie Zheng,Yang Gu,Xiwen Xing,Jiahai Zhou
The L-proline analogue, L-azetidine-2-carboxylate (L-AZC), is of considerable interest both from biological and medicinal chemistry perspectives. It can be quickly involved in proteins and cause protein misfolding, disrupting the normal function of the protein. L-AZC can be biodegraded as the only carbon and nitrogen source by bacteria, and the hydrolysis on the ring opening of L-AZC has an effective practical detoxification function. We identified an L-AZC hydrolase from Novosphingobium sp. MBES04 (NsA2CH), which belongs to the haloacid dehalogenase-like superfamily and participates in cyclic amino acid metabolism. This enzyme has high substrate and stereospecificity for the hydrolysis of L-AZC. We determined the high-resolution crystal structures of NsA2CH in the form of apo- and covalent complexes with the reaction intermediate. Detailed biochemical, structural, and computational studies provide the molecular mechanism of the substrate and stereoselectivity of NsA2CH. Further bioinformatics analysis revealed 328 NsA2CH homologues (sequence identity more than 60%) widely distributed in bacteria. Our work provides the first structural insight into A2CH covalently complexed with the reaction intermediate. The results will enable the engineering of L-AZC to the building block of aminoglycoside antibiotics.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Li Gao,Piao Xu,Jiaoyan Ren
This work described a sensitive and economical HPLC-UV method with FDAA derivatization to simultaneously detect 36 D/ l -amino acids, which provides higher sensitivity and lower cost than other HPLC-based methods. It was validated for linearity range (8–1000 µmol/L), limits of detection (2.68–62.44 pmol/L), limits of quantification (2.93 to 208.13 pmol/L), intraday precision (0.30 % − 5.31 %), interday precision (1.96 % − 8.04 %) and accuracy (86.53 % − 121.46 %). This method was then applied in the determination of D/L- amino acids abundance in fermented and unfermented food materials and showed the characteristics of each type of foods. The method also demonstrated good performance in another application case for the discrimination of different types of food materials based on D/L- amino acids profile. It emphasizes the ability of the method to study the characteristics, distribution and abundance of d -amino acids in foods and their potential application in food quality control.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTIK
·
2022
·
IF 2.84
Xiang-Lin Fang,Rui-Tong Zhao,Na Chen,Xin Wang,Peng Wang,Zhi-Wei He,Shao-Xin Li,Yang Xu,Yong-Hong He
As one of important optical properties of chiral molecules, the real-time monitoring of the optical rotation is of great significance to study the reaction characteristics of chiral molecules. In this study, we have built an optical frequency domain weak measurement system with linear common-path method for chiral sensing, in which the response between optical rotation and central wavelength shift can reach more than 1200 nm/rad. The high-precision measurement of the optical rotation of chiral molecules and the high-sensitivity real-time monitoring of the decomposition rate in the acidic hydrolysis of sucrose under different concentrations of catalysts are realized using this system. The experimental results are basically consistent with the theoretical prediction. This study shows that optical weak measurement chiral sensing can monitor the hydrolysis degree of chiral molecules in real time without separating and labeling molecules. It has great application potential in the production and research of chiral compounds, especially chiral medicine.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Chengyi Xie,Liancheng Gu,Qidi Wu,Lei Li,Chenlu Wang,Jiancheng Yu,Keqi Tang
Chiral analysis is critical to many research fields due to different biological functions of enantiomers in living systems. Although the use of ion mobility spectrometry (IMS) has become an alternative technology in the area of chiral measurements, there is still a lack of a general chiral selector for IMS-based chiral recognition, especially for small chiral molecules. Here, a new method using oligosaccharides as the chiral selector has been developed to discriminate chiral amino acids (AAs) by trapped ion mobility spectrometry-mass spectrometry (TIMS-MS). We analyzed 21 chiral amino acids, including small molecules (e.g., alanine and cysteine). Our data showed that the use of nonreducing tetrasaccharides was effective for the separation of chiral AAs, which differentiated 21 chiral AAs without using metal ions. By further incorporating a copper ion, the separation resolution could be improved to 1.64 on average, which accounts for an additional 52% improvement on top of the already achieved separation in metal-free analysis. These results indicate that the use of tetrasaccharides is an effective strategy for the separation of AA enantiomers by TIMS. The method developed in this study may open up a new strategy for effective IMS-based chiral analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Bingbing Liu,Xiaoya Wang,Yunyun Zhai,Zulei Zhang,Haiqing Liu,Lei Li,Herui Wen
Due to its nano-thickness, fast mass transfer and excellent electron transfer capability, two-dimensional (2D) MOF are an efficient strategy to develop good electrocatalytic materials. In this paper, 2D-MOF were synthesized by facile and cost-efficient methods. MOF can be fast prepared with nucleation and crystal growth in several minutes at room temperature , leading to very thin 2D Co-MOF. Herein, Co, Ni and NiCo-based 2D MOF as non-enzymatic H 2 O 2 sensors at different oxidation potentials in KOH solution, the Co-MOF exhibited excellent H 2 O 2 oxidation and detection performance including a wide linear range from 0.5 μM to 832.5 μM, low detection limit of 0.69 μM, and very fast sensing response in basic solution. By taking advantage of excellent conductivity, the Co-MOF showed the low oxidation potential for H 2 O 2 and good detection performance at 0.25 V. It was comparable to most carbon materials and reported MOF materials for H 2 O 2 detection. We insist that 2D MOF is effective for creating active catalysts. The 2D Co-MOF performed higher electrochemical sensitivity with high stability, conductivity, and selectivity. It indicates 2D MOF has potential employment in the fabrication of sensing devices.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Detao Li,Zichen Ning,Zhuoshan Gong,Limin Zhou,Li Xu,Feiqiang He,Hamza Shehzad,Heng Jerry,Shichao Du,Jinbo Ouyang
According to in-depth research on cocrystals of polyhydroxylated natural products, we previously summarized the substitution model to elucidate the formation mechanism of cocrystals. However, when refers to salt formation of natural products, the substitution model reveals numerous deficiencies. To compensate for the shortcomings of substitution model in predicting salt formation, this study conducts a comprehensive exploration of natural product salts. By supplementing the energy difference rule to the substitution model, it endows the modified model with broader applicability. Simultaneously, the transfer of protons within salts structures is verified by this modified model. Furthermore, we apply competitive cocrystallization technique to separate flavonoid natural products (quercetin, hesperetin, and naringenin). In view of the characteristics of various natural products as well as their cocrystals/salts, numerous separation processes are developed, and the separation effects of these processes are analyzed in detail. The purity of the products obtained from separation process all reaches above 70%, strongly demonstrating the feasibility of using cocrystallization for the binary separation of flavonoids. This work not only refines the substitution model for predicting cocrystals/salts formation of natural products but also indicates the promising application prospects of cocrystallization in industrial separation of natural products.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xudong Zhu,Yanbo Zeng,Zulei Zhang,Yiwen Yang,Yunyun Zhai,Hailong Wang,Lingyu Liu,Jian Hu,Lei Li
Molecularly imprinted polymers prepared using traditional functional monomers and cross-linkers exhibit slow binding kinetics , low electrocatalytic activity and adsorption capacity . Herein, we report a new composite of ionic liquid-based graphene and molecularly imprinted polymer (IL-GR-MIP) with high electrocatalytic activity and adsorption capacity to construct an effective electrochemical sensor for 6-benzylaminopurine (6-BAP). Our objective was to enhance the efficiency of the sensor by incorporating more IL in the MIP framework. We synthesized IL-GR-MIP using ionic liquid 1-vinyl-3-butylimidazolium tetrafluoroborate (IL 1 ) as functional monomer, ionic liquid 1,4-butanediyl-3,3′-bis-l-vinylimidazolium dibromide (IL 2 ) as cross-linker, 6-BAP as template, and GR as supporter. IL-GR-MIP was characterized by Fourier transform infrared spectroscopy, thermal gravimetric analysis , Raman spectroscopy , X-ray photoelectron spectroscopy, and scanning electron microscope . Compared with GR-MIP composites based on methacrylic acid or IL 1 as functional monomer, N, N'-methylenebisacrylamide and ethylene glycol dimethacrylate as cross-linker, the IL-GR-MIP (prepared with ionic liquids as functional monomer and cross-linker) sensor exhibited highest peak current for 6-BAP. The results indicate the ability of IL 2 as cross-linker to enhance electrocatalytic activity and adsorption capacity for 6-BAP of IL-GR-MIP. Under the optimized conditions, the peak current of IL-GR-MIP sensor was linear to 6-BAP concentration in the range of 0.5–50 μM with a detection limit of 0.2 μM (S/N = 3). The IL-GR-MIP sensor exhibited good selectivity with the anti-interference ability of 1000-fold ascorbic acid in 6-BAP determination. Furthermore, we demonstrated practical applicability of IL-GR-MIP sensor in detecting 6-BAP in real samples with satisfactory results.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）