Molecular Catalysis
·
2024
·
IF 3.9
Yun Zhang,Guangfei Qu,Zhishuncheng Li,Junhong Zhou,Rui Xu,Junyan Li
This research has made significant advancements in the field of high-value bio-oil production, specifically focusing on the synthesis of the rare sugar D-Allose. Notably, synthesizing D-Allose through cellulose pyrolysis has long been challenging due to its high synthetic difficulty, which has limited related studies. By employing a one-pot synthesis approach using Al x -Fe y nano-catalysts prepared through a unique cellulose pretreatment method, this experiment successfully achieved efficient generation of D-Allose during rapid pyrolysis. Systematic experimentation demonstrated that at 330 °C with the Al 1 -Fe 5 nano-catalyst, the yield of D-Allose from cellulose pyrolysis reached an impressive 50.69 %, accompanied by a detection of 14.35 % furfural in the bio-oil. This breakthrough not only significantly contributes to the high-value utilization of bio-oil but also provides robust support for further development in this area. Simultaneously, the success of this research extends beyond high-value bio-oil utilization and makes a substantial impact on both the scientific community and industry.
研究领域:
生物化学
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可再生资源利用
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催化化学
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产品关联
DOI
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Zheng Qian,Long Si,Chen Zhi,Fu Jiaolong,Ju Xin,Li Liangzhi
Enzymatic preparation of rare sugars as an alternative to traditional sweeteners is an effective strategy to achieve a low-calorie healthy diet. Ribose-5-phosphate isomerase B (RpiB) is a key enzyme in the non-oxidative branch of the catalytic pentose phosphate pathway. Here, we investigated the potential of Curtobacterium flaccumfaciens ZXL1 ( C. flaccumfaciens ZXL1) derived RpiB (CfRpiB) in D-allose preparation. The optimal reaction conditions for recombinant CfRpiB were found experimentally to be pH 7.0, 55 °C, and no metal ions. The kinetic parameters K m , k cat , and catalytic efficiency k ca t / K m were 320 mM, 4769 s −1 , and 14.9 mM −1 s −1 respectively. The conversion of D-allulose by purified enzyme (1 g L −1 ) to D-allose was 13% within 1 h. In addition, homology modeling and molecular docking were used to predict the active site residues: Asp13, Asp14, Cys72, Gly73, Thr74, Gly77, Asn106, and Lys144.
研究领域:
生物化学
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生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Shuangshuang Duan,Yonghua Chen,Guodong Wang,Zebin Li,Shitong Dong,Yingshuai Wu,Yuanwei Wang,Chunling Ma,Ruiming Wang
D -Allose is a rare cis-caprose with a wide range of physiological functions, which has a wide range of applications in medicine, food, and other industries. L -Rhamnose isomerase ( L -Rhi) is the earliest enzyme found to catalyze the production of D -allose from D -psicose. This catalyst has a high conversion rate, but its specificity for substrates is limited; thus, it cannot fulfill the requirements of industrial production of D -allose. In this study, L -Rhi derived from Bacillus subtilis was employed as the research subject, and D -psicose as the conversion substrate. Two mutant libraries were constructed through alanine scanning, saturation mutation, and rational design based on the analysis of the secondary structure , tertiary structure , and interactions with ligands of the enzyme. The yield of D -allose produced by these mutants was assessed; it was found that the conversion rate of mutant D325M to D -allose was increased by 55.73 %, and the D325S improved by 15.34 %, while mutant W184H increased by 10.37 % at 55 °C, respectively. According to modeling analysis, manganese (Mn 2+ ) had no significant effect on the production of D -psicose from D -psicose by L -Rhi. The results of molecular dynamics simulation demonstrated that the mutants W184H, D325M, and D325S had more stable protein structures while binding with the substrate D -psicose, as evidenced by its root mean square deviation (RMSD), root mean square fluctuation (RMSF), and binding free energy values. It was more conducive to binding D -psicose and facilitating its conversion to D -allose, providing the basis for the production of D -allose.
研究领域:
生物化学
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酶学
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生物技术
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结构生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Xiaofeng Zhang,Xinqi Xu,Xuemei Yao,Rong Wang,Hengtao Tang,Xin Ju,Liangzhi Li
Ribose-5-phosphate isomerase B is of great importance for biocatalysis and biosynthesis, but the multifunctional residues in active sites hinder the research efforts. This study employed rational design strategies to locate the key residues of RpiB from Ochrobactrum sp. CSL1 (OsRpiB). A single-mutant S9T of a noncontact residue showed 80% activity improvement toward d-allose. A double-mutant S98H/S134H further increased the activity to 3.6-fold. The mutations were analyzed by kinetics and molecular dynamics analyses, indicating that S9T might enhance the substrate binding and catalysis by inducing a steric effect, and S98H/S134H could strengthen both ring opening and binding of d-allose. Though S98H/S134H showed low temperature stability, its potential was explored by isomerizing d-allose to d-psicose with higher conversion and in less reaction time. The findings of this study were beneficial for illustrating the complex functions of key residues in RpiBs and applying OsRpiB in preparing rare sugars.
研究领域:
生物化学
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分子生物学
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合成生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Dan Bu,Zong-cai Tu,Hui Wang,Yue-ming Hu,Qing Sun,Guang-xian Liu
The mechanism of d -allose in reducing the allergenicity and digestibility of ultrasound-pretreated α-lactalbumin (α-LA) was studied. The intensity reduction and peak red shift occurred in fluorescence spectra of glycated samples. Enzyme-linked immunosorbent assay and basophil degranulation analysis showed that d -allose significantly reduced the allergenicity of α-LA, and ultrasound-pretreated α-LA showed the lowest allergenicity after glycation. Compared with α-LA, the degree of hydrolysis decreased in glycated samples. Size-exclusion high-performance liquid chromatography showed that the glycated α-LA was resistant to digestive enzymes. The glycated sites and average degree of substitution per peptide molecule were determined using LC Orbitrap MS/MS. These results suggested that the masking of linear allergenic epitopes by glycation could reduce the allergenicity. Therefore, the combination of ultrasound pretreatment and glycation is a potential method to reduce protein allergenicity in food processing and provides a useful approach for the application of rare sugars in food processing.
研究领域:
食品科学
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过敏学
·
质谱学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Bo Zhang,Liqun Shen,Cheng Yu,Zhibo Leng,Zhihong Zhu,Lingfei Hua,Zijie Li
d-Allose, one of important rare sugars, has attracted increasing attention due to its low caloric value and remarkable physiological activities. l-Rhamnose isomerase (L-RI) catalyzes the reversible conversion of d-allulose to d-allose. However, its industrial application is limited by inadequate catalytic efficiency. In this study, L-RI from Clostridium thermosuccinogenes was identified and systematically characterized. Using a semirational protein engineering strategy, loop residues adjacent to the catalytic core and potential active sites predicted by the HotSpot Wizard web server were targeted. The resultant triple mutant N137Q/D258R/A265I achieved a d-allose conversion rate of 21.8% and a productivity of 130.8 g·L–1·h–1 within 1 h using 600 g·L–1 d-allulose. Molecular dynamics (MD) simulations revealed that modulation of loop flexibility enhanced the enzyme turnover rate. By fine-tuning loop flexibility while maintaining the structural integrity of the enzyme, this engineered mutant reduces the risk of enzyme inactivation during prolonged high-temperature catalysis. Collectively, these results highlight its potential for industrial d-allose production and provide valuable insights for the modification of L-RIs and related enzymes.
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xinrui Tang,Abdullah Arsalan,Guoyan Zhang,Junhua Yun,Cunsheng Zhang,Xianghui Qi
D-Allose, a rare sugar, has gained significant attention not only as a low-calorie sweetener but also for its anticancer, antitumor, anti-inflammatory, antioxidant, and other pharmaceutical properties. Despite its potential, achieving high-level biosynthesis of D-allose remains challenging due to inefficient biocatalysts, low conversion rates, and the high cost of substrates. Here, we explored the food-grade coexpression of Blautia produca D-allulose 3-epimerase (Bp-DAE) and Bacillus subtilis L-rhamnose isomerase (BsL-RI) within a single cell using B. subtilis WB800N as the host. Using this system, D-allose was synthesized via a simple, cost-effective, one-pot enzymatic process, employing whole cells as catalysts and D-fructose as the substrate. The system exhibited optimal activity at 65 °C, pH 8.5, with 1 mM Mn2+ and 20 g/L of whole-cell dry weight. Initial production reached 12.5 g/L D-allose with a 12.5% yield from 100 g/L D-fructose. Optimization of dual promoter combinations enhanced production, achieving 15.0, 29.1, and 43.2 g/L D-allose from 100, 200, and 300 g/L D-fructose, with yields of 15.00, 14.55, and 14.40%, respectively. This D-allose production biocatalyst offers a scalable and economically viable platform for the industrial production of rare sugar.
研究领域:
生物化学
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食品科学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2025
·
IF 9.8
Xiaofeng Song,Mengbin Gu,Jun Ding,Weibin An,Zhe Wang,Weiguang An
This study investigates the development of eco-friendly foam extinguishing agents for suppressing thermal runaway (TR) fires in lithium-ion batteries (LIBs) by synergizing hydrocarbon surfactants. Three anionic surfactants—sodium α-alkenyl sulfonate (AOS), sodium dodecyl benzene sulfonate (SDBS), and sodium lauryl ethoxylate sulfate (SLES)—were compounded with amphoteric betaine (Bet) and nonionic alkyl polyglucoside (APG) to optimize foamability, stability, wettability, and fire suppression performance. Surface tension analysis, critical micelle concentration (CMC) measurements, and fire extinguishing tests were conducted to evaluate the physicochemical properties and mechanisms of these composite systems. Results demonstrated that Bet/SDBS and APG/SLES formulations exhibited superior synergistic effects, significantly reducing surface tension (e.g., Bet/SDBS: γ CMC = 29.38 mN/m) and enhancing foamability, wettability and foam stability. In fire suppression tests, the 0.1 wt% Bet/0.1 wt% SDBS system achieved the fastest extinguishing time (0.21 s) and highest cooling rate (20.7 °C/s) by forming a dense foam layer that isolated oxygen and prolonged cooling. The study highlights the potential of hydrocarbon surfactant blends as sustainable alternatives to fluorocarbon-based agents, offering biodegradability, low toxicity, and high efficiency in LIB fire scenarios.
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Weiwei Han,Tianyu Shi,Chao Ma,Chenwei Liu,Sanbao Dong
Foam drainage is a critical technique for enhancing gas production in water-producing natural gas wells during mid-to-late operational stages. In this study, we hypothesized that constructing a pseudo-Gemini supramolecular surfactant complex through non-covalent interactions between a hydroxy tertiary amine (HTA) and 2-hydroxyterephthalic acid (HTPA) would significantly enhance foam stability and liquid-carrying performance under harsh downhole conditions. To realize this, we developed a novel five-component foam drainage agent by integrating the HTA/HTPA complex with cocamidopropyl betaine (CAPB), α-olefin sulfonate (AOS), and sodium carboxymethyl cellulose (NaCMC). The optimized molar ratio and total dosage of HTA:HTPA:AOS:CAPB:NaCMC were found to be 12:6:42:40:25 and 15 mM/L respectively, at which this foaming system exhibited a foaming volume of 390 mL and a half-life time of 23.07 min. Critically, liquid-carrying rate tests demonstrated that moderate contents of condensate and methanol enhanced drainage efficiency, the presence of 30 % condensate yielded a liquid-carrying rate of 43.06 %, while the presence of 20 % methanol increased it to 47.10 %. Remarkably, the system maintained 100 % foaming efficiency over a wide range of methanol or condensate concentrations (0–50 % v/v). This foaming system also exhibited excellent tolerance to salinity (up to 300 g/L, enabled by nitrilotriacetic acid) and temperature (up to 90 ℃), at which produced a liquid-carrying rate of 91.98 % while foaming efficiency remained at 100 % across 20–90 ℃. Besides, surface tension measurements, shear viscosity tests, microscopic foam morphology analysis, interaction energy calculations, and electrostatic surface potential analyses confirmed strong synergistic effect among the components. These findings validate the rational design of the supramolecular system and underscore its high potential as a novel foam drainage agent for efficient gas well deliquification.
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 9
·4248
·
IF 4.9
Yongchen Yu,Xiaona Qian,Xiwang Li,Zhichao Chai,Dejiang Ni,Xiaoling Sun
The jasmonic acid (JA) signaling pathway has been demonstrated to play a crucial role in plant defense against herbivorous insects. However, the relationship betweenEctropis obliqua-induced defensive metabolites and the JA signaling pathway in tea plants remains poorly understood. In this study, we investigated seven key special metabolites, includingp-coumaroylputrescine, feruloylputrescine, prunin, naringenin, and three monolignols, to address this knowledge gap. Epicatechin was selected as a positive control based on its well-documented regulation through the JA signaling pathway. Notably, the content of all selected compounds was significantly increased byE. obliquainfestation. Furthermore, exogenous application of high-dose methyl jasmonate (MeJA) induced the accumulation of six of the eight compounds, excludingp-coumaryl alcohol and sinapyl alcohol, whereas low-dose MeJA failed to elicit their accumulation. To confirm the results, we screened two bioactive molecules, D-allose and L-theanine, which significantly increased the endogenous JA levels at low concentrations. Interestingly, neither D-allose nor L-theanine triggered the biosynthesis of these defensive compounds. Additionally, D-allose-treated tea leaves had no significant effect on the performance ofE. obliqualarvae. These findings demonstrate that the metabolic accumulation induced byE. obliquais mediated through a high-threshold JA signaling cascade. This study provides novel insights into the relationship between plant resistance and JA signaling pathway, advancing our understanding of special metabolites mediated plant-insect interactions.
研究领域:
植物学
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昆虫学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Liyuan Wu,Yaxin Liu,Xin Wang,Mengrui Li,Jingya Li,Xiaoran Zhang,Dawen Gao,Haiyan Li
Benzo[a]pyrene is difficult to remove from soil due to its high octanol/water partition coefficient. The use of mixed surfactants can increase solubility but with the risk of secondary soil contamination, and the compounding mechanism is still unclear. This study introduced a new approach using environmentally friendly fatty acid methyl ester sulfonate (MES) and alkyl polyglucoside (APG) to solubilize benzo[a]pyrene. The best result was obtained when the ratio of MES/APG was 7:1 under 6 g/L total concentration, with an apparent solubility (Sw) of 8.58 mg/L and a molar solubilization ratio (MSR) of 1.31 for benzo[a]pyrene, which is comparable to that of Tween 80 (MSR, 0.95). The mechanism indicates that the hydroxyl groups (−OH) in APG form “O–H···OSO2–” hydrogen bonding with the sulfonic acid group (−SO3–) of MES, which reduces the electrostatic repulsion between MES molecules, thus facilitating the formation of large and stable micelles. Moreover, the strong solubilizing effect on benzo[a]pyrene should be ascribed to the low polarity of ester groups (−COOCH3) in MES. Functional groups capable of forming hydrogen bonds and having low polarity are responsible for the enhanced solubilization of benzo[a]pyrene. This understanding helps choose suitable surfactants for the remediation of PAH-contaminated soils.
研究领域:
环境科学
·
化学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Hongbin Yang,Haocong Li,Haizhuang Jiang,Yubin Zhang,Luyao Xing,Xin Kang,Huazheng Wang,Shuhe Zhang,Haobin Shi,Wanli Kang
Nanopolymer microspheres have been widely studied in the development of low-permeability reservoirs as an effective material to improve foam stability. However, there are few studies on the foam stability mechanism of nanopolymer microspheres. In this work, the effect of surface hydrophobicity of fluorescent nanopolymer microspheres PARC(Flu-Ac) on the stability of CO2 foam was investigated by using foam performance as an evaluation index. The mechanism of foam stabilization by PARC(Flu-Ac) with different degrees of hydrophobicity was clarified by studying the drainage rate, micromorphology, interfacial rheology of foam, and adsorption behavior of microspheres on the foam liquid film. The results show that PARC(Flu-Ac)-5 microspheres with neutral wettability have the best foam stabilization effect. At 94 °C, the foam volume of CO2 foam is 630 mL and the foam half-life is 30.12 min. PARC(Flu-Ac)-5 microspheres have a high desorption energy and can stably adsorb at the gas–liquid interface to form a solid layer. The microspheres improve the mechanical strength of the foam and reduce the direct contact of the fluid, thus providing space resistance for thinning the liquid film and the diffusion of gas between bubbles. In addition, the microspheres can slow the thinning rate of the liquid film through the release of free water. The stability of the foam is significantly improved. This study further clarifies the enhancement mechanism of nanopolymer microspheres on the stability of CO2 foam, which has excellent guiding significance for the application of CO2 foam in low-permeability oil fields.
研究领域:
石油工程
·
材料科学
·
界面化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）