ANALYST
·
2020
·Vol. 145
·期 12
·4254-4259
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IF 3.978
Qingqing Zhang,Tong Yang,Guoxiang Zheng,Heng Gao,Chenxiao Yan,Xiong Zheng,Xiaoshun Zhou,Yong Shao
G-triplex (G3) has been recognized as a popular intermediate during the folding of G-quadruplex (G4). This has raised interest to anticipate the ultimate formation of G3 by shortening the G4-forming oligonucleotides with the remaining three G-tracts. Some G3 structures have been validated and their stability has been found to be affected by the loop sequences similar to G4s. In this work, however, we first found that an intermolecular parallel G4 structure was preferred in K+ for the oligonucleotide 5′-TGGGTAGGGCGGG-3′ (DZ3) containing only three G-tracts. We screened auramine O (AO) as the appropriate fluorophore with a molecular rotor feature to target this G4 structure. AO bound with DZ3 in a 1 : 4 ratio, as confirmed by isothermal titration calorimetry experiments, suggesting the formation of a tetramolecular G4 structure (4erG4). The excimer emission from the labelled pyrene and the DNA melting behavior at various pHs in the presence of Ag+ proved the formation of the 4erG4 structure rather than the prevalent intramolecular G3 folding. This work demonstrates that one should be cautious while putatively predicting a G3 structure from an oligonucleotide containing three G-tracts.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 29
·13931-13939
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IF 3.925
Nan Sun,Yuhan Jing,Yong Zhang,Xinru Li,Yaoning Bai,Yuwei Yan,Taoyuan Ouyang,Honglin Tan,Xiaoming Cai,Jinming Cai
Copper-based photocatalysts have attracted much attention because of their great prospects in photochemical conversion. However, the preparation of highly stable copper-based photocatalysts is a challenge. In this study, copper nanoparticles (Cu NPs) wrapped in graphitized polyacrylonitrile (g-PAN) were prepared by a two-step sintering method. Even if Cu@g-PAN is placed in the air for one month, the Cu NPs were still not oxidized. Furthermore, we succeeded in further introducing graphitized carbon nitride (g-C3N4) into the g-PAN shell structure, which was confirmed using a Scanning Transmission Electron Microscope (STEM), FT-IR, and UV-Vis absorption spectra. Cu@g-PAN/g-C3N4 can degrade 88.61% of methylene blue (MB) in one hour, showing a higher catalytic activity than Ag@g-PAN/g-C3N4 (55.18%). XPS and Auger electron spectroscopy tests of catalyzed Cu@g-PAN/g-C3N4 show that Cu is not oxidized. We have proposed a possible mechanism for charge separation and transformation in the Cu@g-PAN/g-C3N4 composites to explain the enhanced photocatalytic performance. This work provides a new method to protect Cu NPs and demonstrates the prospect of rational design of copper-based photocatalysts with high stability and efficiency.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Jingle Zhang,Huihu Wang,Yangliu Ou,Hao Tu,Ziguan Zhang,Chenhuinan Wei,Xing Xiang,Ming Bao
As the overuse of antibiotics increasingly contaminates the environment, one of modern chemistry's objectives is to identify high-performance photocatalytic materials to degrade antibiotics. This study initially utilized NH 4 Cl as a gas template to synthesize porous lamellar g-C 3 N 4 (CNS) through a high-temperature calcination process. Building upon this, a homojunction catalyst, CNS/g-C 3 N 5 (S-CN), was developed via a secondary sintering technique using different amount of 3-amino-1,2,4-triazole addition to evaluate its efficacy in activating peroxymonosulfate (PMS) for degrading the antibiotic ceftriaxone sodium. Under neutral conditions, the photocatalytic system composed of S-CN catalysts prepared by 50 mg of 3-amino-1,2,4-triazole addition and PMS (S 3 -CN/PMS) exhibited degradation rates that were 3.37 and 8.11 times higher than those of the CNS/PMS and g-C 3 N 5 /PMS, respectively. At a pH of 8, the S 3 -CN/PMS system attained a 99.7 % degradation efficiency within 75 min. Based on results from UV–visible diffuse reflectance spectroscopy (DRS), transient photocurrent response, electrochemical impedance spectroscopy (EIS), photoluminescence spectroscopy and radical scavenging experiments, this high efficiency is attributed to a homojunction Z-type charge transfer mechanism that enhances the separation of photogenerated carriers. Furthermore, the study found that the optimal PMS addition was 20 mg and the optimal pH was 8. Chloride ions promoted the reaction, while other ions decreased the reaction rate. In conclusion, this paper provides valuable insights for the morphological modification of carbon nitride homojunction materials and the efficient activation of PMS for pollutant degradation.
研究领域:
环境科学
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材料科学
·
化学工程
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DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 40
·7413-7419
·
IF 1.915
Min Huang,Hanying Li,Hanping He,Xiuhua Zhang,Shengfu Wang
The sequence-specific recognition of SNPs is important for disease diagnosis and human gene therapy. In this work, a simple sensor based on electrochemical impedance spectroscopy (EIS) was developed for the highly sensitive and specific detection of G–G mismatches in dsDNA using a small molecule-modified gold electrode. After the electrochemical impedance sensor was incubated with G–G mismatched dsDNA, its charge-transfer resistance (Rct) increased significantly. Meanwhile, only a small increase in Rct values was observed for dsDNA with other base mismatches and for complementary dsDNA. Importantly, G–G mismatched dsDNA was selectively detected based on differences in charge transfer resistance (ΔRct) even in the presence of other DNA and ct-DNA. Under optimal experimental conditions, the established EIS sensor was used to quantify G–G mismatched dsDNA based on the linear relationship between ΔRct and the logarithm of the concentration of G–G mismatched dsDNA from 1 nM to 1 μM with a detection limit of 0.3 nmol L−1. This sensing strategy is a potential alternative tool for measuring G–G mismatched DNA for the diagnosis and early clinical detection of genetic diseases.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
JOURNAL OF MATERIALS RESEARCH
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2020
·Vol. 35
·期 16
·2148-2157
·
IF 2.502
Qianhong Shen,Chengyan Wu,Zengyu You,Feilong Huang,Jiansong Sheng,Fang Zhang,Di Cheng,Hui Yang
A novel g-C3N4 nanoparticle@porous g-C3N4 (CNNP@PCN) composite has been successfully fabricated by loading g-C3N4 nanoparticles on the porous g-C3N4 matrix via a simply electrostatic self-assembly method. The composition, morphological structure, optical property, and photocatalytic performance of the composite were evaluated by various measurements, including XRD, SEM, TEM, Zeta potential, DRS, PL, FTIR, and XPS. The results prove that the nanolization of g-C3N4 leads to an apparent blueshift of the absorption edge, and the energy band gap is increased from 2.84 eV of porous g-C3N4 to 3.40 eV of g-C3N4 nanoparticle (Fig. 6). Moreover, the valence band position of the g-C3N4 nanoparticle is about 0.7 eV lower than that of porous g-C3N4. Therefore, the photo-generated holes and electrons in porous g-C3N4 can transfer to the conduction band of g-C3N4 nanoparticle, thereby obtaining higher separation efficiency of photo-generated carriers as well as longer carrier lifetime. Under visible-light irradiation, 6CNNP@PCN exhibits the highest photocatalytic performance (Fig. 8) on MB, which is approximately 3.4 times as that of bulk g-C3N4.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 10
·3330
·
IF 4.927
Bingxin Mao,Cong Liu,Xu Cui,Yanhui Li,Qian Duan
A novel thermo-responsive 2,9(10),16(17),23(24)-tetrakis[(3-carboxyacrylamide) phthalocyaninato] zinc (ZnPc)-g-TiO2-g-poly(N-isopropylacrylamide) (PNIPAM) photocatalyst modified with phthalocyanines was prepared. The photocatalyst exhibited thermo-responsive properties due to the introduction of PNIPAM, which performed recovery for reuse above the lower critical solution temperature (LCST, about 26 °C). ZnPc-g-TiO2-g-PNIPAM effectively expanded the light response range to the visible light region and inhibited the recombination of electron–hole pairs, which enhanced the performance of the photocatalyst. As expected, ZnPc-g-TiO2-g-PNIPAM (0.3 g/L) exhibited excellent photocatalytic performance for the removal of Rhodamine B (RhB, 1.0 × 10−5mol/L) and methylene blue (MB, 1.0 × 10−5mol/L) under visible light, which reached 97.2% and 88.6% at 20 °C within 40 min, respectively. Furthermore, the influence of temperature upon photocatalytic performance was also investigated. When the temperature increased from 20 °C to 45 °C, the removal of RhB decreased by approximately 53.8%. The stability of the photocatalyst demonstrated that the photocatalytic activity was still above 80% for the removal of RhB after 3 cycles. Above all, this work provided an intelligent thermally responsive photocatalyst based on phthalocyanine for water purification under visible light.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2021
·
IF 19.503
Rongdi Tang,Daoxin Gong,Yaoyu Zhou,Yaocheng Deng,Chengyang Feng,Sheng Xiong,Ying Huang,Guanwei Peng,Ling Li
Herein, a g-C 3 N 4 /PDI-g-C 3 N 4 homojunction has been fabricated for piezo-photocatalytic atrazine removal and exhibited better performance than individual photocatalysis or piezocatalysis. The introduction of PDI induces the π-π interaction facilitating electrons migration, and twists the g-C 3 N 4 plane into a more polar porous structure with enhanced piezoelectricity. The homojunction facilitates the photoelectron transfer at the g-C 3 N 4 /PDI-g-C 3 N 4 interfaces. The photoelectricity and the piezoelectricity of g-C 3 N 4 /PDI-g-C 3 N 4 were assessed. The finite element simulation showed that the porous structure of the g-C 3 N 4 /PDI-g-C 3 N 4 is essential to the enhanced piezoelectricity. Astonishingly, the piezo-photocatalytic atrazine degradation rate under an optimized condition (pH=2.97) reached 94% within 60 min. Moreover, the g-C 3 N 4 /PDI-g-C 3 N 4 homojunction produced 625.54 μM H 2 O 2 during the one-hour piezo-photocatalysis. Given the quenching experiments, reactive species detection and the electronic band of g-C 3 N 4 /PDI-g-C 3 N 4 , the piezo-photocatalytic mechanism has been proposed. In addition, the degradation pathways and the reduced intermediates toxicity intermediates of atrazine have been investigated.
研究领域:
环境科学
·
材料科学
·
催化化学
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DOI
高影响因子
Catalysts
·
2023
·Vol. 13
·期 11
·1398
·
IF 3.9
Wei Qian,Yi Fang,Hui Liu,Yili Deng,Yingying Li,Yongzheng Zhang,Zenghui Diao,Mingyu Li
The environmental pollution caused by antibiotics is becoming more serious. In this study, the Mn/BiPO4/g-C3N4composite (Mn-BPC) and the Ti/g-C3N4/BiPO4composite (Ti-BPC) were prepared by hydrothermal reaction method and solvent method, respectively, and applied to the degradation of tetracycline (TC) in an aqueous environment. The XRD and HRTEM results showed that these materials had the crystalline rod-like structure of BiPO4and abundant carbon, nitrogen and carbon–oxygen surface functional groups. The degradation of TC by Ti-BPC and Mn-BPC were nearly 92% and 79%, respectively. The degradation processes of TC were well consistent with the pseudo-second-order kinetics model and R2values were closer to 1. The trapping experiment showed that electron holes (h+) were the main reactive species for the degradation of tetracycline, OH· and O2−also have certain effects. Also, the possible photocatalytic degradation mechanism of Ti-BPC and Mn-BPC composites was thereby proposed. TC was firstly adsorbed on the surface of catalysts, and subsequently degraded by reactive species such as h+, OH· and O2−generated under visible light excitation. This study shows that the Ti-BPC and Mn-BPC photocatalysts have great potential in antibiotic degradation and can provide new ideas for antibiotic removal in aqueous environments.Keywords:bismuth phosphate;graphite phase carbon nitride;tetracycline (TC)
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Huijie Li,Jingli Zhao,Yan Geng,Zhongjun Li,Yike Li,Jianshe Wang
CoP/g-C 3 N 4 , a kind of noble-metal-free binary photocatalyst , has been demonstrated to be active for water splitting. However, high recombination rate of charge carriers is still one of the biggest factors to restrict the photoactivity of CoP/g-C 3 N 4 . In this paper, Boron doped g-C 3 N 4 nanodots (BCNDs) were introduced to CoP/g-C 3 N 4 nanosheets (CoP/CNNS) and a novel CoP/BCNDs/CNNS ternary photocatalyst was constructed. Experimental characterization and testing results indicate that BCNDs/CNNS heterojunction was formed in CoP/BCNDs/CNNS ternary photocatalyst. Comparing with CoP/g-C 3 N 4 , CoP/BCNDs/CNNS facilitates electron-hole separation, and the photocatalytic hydrogen evolution rate can reach 1332.81 μmolg −1 ·h −1 , which is approximately 9.7 times as high as CoP/CNNS. It owns an apparent quantum efficiency of 10.78% at 420 nm. This work would provide a novel strategy to construct high activity g-C 3 N 4 based catalyst without noble metals.
研究领域:
材料科学
·
化学
·
纳米技术
·
能源科学
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DOI
高影响因子
Microbiology Spectrum
·
2022
·Vol. 10
·期 3
·
IF 9.043
Lu Lv,Leiliang Zhang
Human enteroviruses cause many diseases; however, there is no specific therapeutic drug. G-quadruplex is an atypical secondary structure formed in the guanine rich region of DNA or RNA, which can exist in the viral genome. The different positions of G-quadruplex play an important role in the regulation of virus replication and infection. Whether G-quadruplexes are present in human enteroviruses is unknown. In current study, we analyzed the potential quadruplex forming sequences of human enteroviruses, especially EV-A71 virus, which causes hand, foot, and mouth disease. The results showed that there were a certain number of potential quadruplex-forming sequences in human enteroviruses. Through a variety of experimental methods, we evaluated the formation potential of EV-A71 encoded G-quadruplex and analyzed the binding ability of G-quadruplex ligands, including BRACO-19, pyridostatin and TMPyP4 to virus encoded G-quadruplexes. G-quadruplex ligands BRACO-19, PDS and TMPyP4 could inhibit the transcription of constructs containing EV-A71 G-quadruplex sequences. Moreover, we found that BRACO-19 was able to inhibit the replication of EV-A71, suggesting that targeting G-quadruplexes in EV-A71 genome by G-quadruplex ligands could be a novel antiviral way against EV-A71. Our finding not only uncovered the G-quadruplexes in human enteroviruses, but also would provide a new strategy for human enteroviruses therapy.IMPORTANCE G-quadruplex is a stable nucleic acid secondary structure formed by the folding of guanine rich nucleic acid. The important regulatory function of G-quadruplex makes it an attractive target of antiviral effect. Human enteroviruses cause a variety of human diseases, including common cold, nervous system diseases, cardiovascular damage, and diabetes. Enterovirus A71 (EV-A71) is one of pathogens causing hand, foot, and mouth disease; however, whether G-quadruplexes are present in the genomes of human enteroviruses is unknown. The function of G-quadruplexes in the EV-A71 genomes is not clear. We predicted and characterized G-quadruplex sequences in EV-A71. G-quadruplex ligands were identified to stabilize EV-A71 G-quadruplexes with high affinities. We also demonstrated G-quadruplex ligand BRACO-19 inhibited EV-A71 replication. Our studies provide a framework for targeting G-quadruplexes in the enteroviruses genome, which will be a new way to develop antiviral agents against human enteroviruses.
研究领域:
分子生物学
·
病毒学
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药理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Min Zou,Jing-Yan Li,Meng-Jia Zhang,Jun-Hui Li,Jun-Tao Huang,Pei-Dan You,Shu-Wen Liu,Chun-Qiong Zhou
At present, there are still no anti-Zika virus (ZIKV) drugs or vaccines approved by FDA with accurate targets and antiviral mechanisms. Considering the RNA G-quadruplex sequences in ZIKV genome, it is very meaningful to develop G-quadruplex binders as potential anti-ZIKV drugs with novel and accurate targets. In this paper, five classical G-quadruplex binders including Ber , Braco 19 , NiL , 360A and PDS have been chosen to discuss their interaction with ZIKV RNA G-quadruplexes. PDS shows higher binding affinity and thermal stability than the other G-quadruplex binders. This property is further evidenced in cells by immunofluorescence microscopy. And PDS shows higher anti-ZIKV activity (EC 50 = 4.2 ± 0.4 μM) than the other G-quadruplex binders as well as the positive control ribavirin, with a low cytotoxicity. By time-of-addition assay, PDS exerts antiviral activity at the post-entry process of ZIKV replication cycle, thus inhibiting ZIKV mRNA replication and protein expression. Furthermore, PDS combines with ZIKV NS2B-NS3 protease and reduces its catalytic activity. This study suggests that G-quadruplex binder PDS is an effective multi-target ZIKV inhibitor, which provides more guidance to design some novel anti-ZIKV drugs targeting ZIKV RNA G-quadruplexes.
研究领域:
病毒学
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药物化学
·
分子生物学
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DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Zeyi Wang,Yuzhen Qian,Guozhen Wang,Xinyu Sun,Jingcheng Hao
Supramolecular ionogels, as an emerging class of materials, utilize the intriguing properties of ionic liquids (ILs) and offer a promising method for constructing functional materials in anhydrous solvents. G-Quadruplex, assembled from guanine-rich nucleic acid building blocks, is an important structure closely associated with life and leads to the gelation for constructing versatile materials, such as the electrolytes of supercapacitors. Herein, we design and fabricate G-quadruplex ionogels using an unreported cation. Among the metal acetylacetonates under investigation, iron(III) acetylacetonate is distinctive in its capacity to stabilize G-quadruplexes through a reaction with the solvent ethylammonium nitrate (EAN), resulting in the formation of Fe(acac)2+. The ionogel displays high ionic conductivity (6.43 mS·cm–1), a wide electrochemical stability window (2.86 V), and self-healing properties. The supercapacitor based on the G-quadruplex ionogel electrolyte delivers a gravimetric capacitance of 91.8 F·g–1, a high energy density of 32.6 W h/kg, and a power density of 319.8 W/kg at a current density of 0.2A·g–1. Therefore, the present findings have important implications for deepening the understanding of G-quadruplex structures and metal acetylacetonate complexes and provide an approach to fabricating ionogel electrolytes for high-performance supercapacitors.
研究领域:
材料科学
·
生物化学
·
能源存储
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）