COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Feng Wang,Chao Li,Haohao Wang,Lu Yu,Fuming Zhang,Robert J. Linhardt
Natural amino acid polymers have been wide-used as bio-compatible emulsion stabilizers . In this study, Amphiphilic O(Phe- r -Glu)s were synthesized through the papain-catalyzed random polymerization of L -phenylalanine and L -glutamate with the major degree of polymerization value of 6–7. The polymerization yield was increased by more than 35 % using choline to reduce the energy barrier for the amino substrates to form the tetrahedral intermediate with the catalytic triad. The squalene/water emulsions stabilized by O(Phe- r -Glu) nanoparticles were pH-insensitive at pH 1–12. The investigation of mechanism found the combined effects of steric hindrance , electrostatic repulsion and the viscosity of the emulsion contributed to the stability of the emulsion droplets. Under acidic condition, the layers of O(Pho- r -Glu)s particle aggregates formed around the emulsion droplets and the higher viscosity of emulsion mainly contributed to the stability of emulsions . At basic condition, the strong electrostatic repulsion between emulsion droplets covered by the negatively charged O(Pho- r -Glu) particles and the steric hindrance contributed mainly to the prevention of the coalescence of emulsion droplets. The application of enzymatically synthesized amphiphilic peptides in emulsion, along with tunable performance through the polymerization of amino substrates, is in line with the sustainable development requirements in fields such as pharmaceuticals, food and cosmetics.
研究领域:
生物材料学
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食品科学
·
制药工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Shan Yu,Ying Gao,Xu Mei,Tanchen Ren,Su Liang,Zhengwei Mao,Changyou Gao
Selective adhesion and migration of endothelial cells (ECs) over smooth muscle cells (SMCs) is very important in the rapid endothelialization of blood-contacting implants to prevent vascular restenosis. In this study, a uniform cell-resistant layer of methacrylate-functionalized hyaluronic acid (HA) was first immobilized on a poly(ε-caprolactone) (PCL) film via polydopamine coupling. Then, a density gradient of thiol-functionalized Arg-Glu-Asp-Val (REDV) peptide was prepared on the HA layer via thiol-ene click chemistry and the continuous injection method. The REDV gradient selectively enhanced EC adhesion and preferential directional migration toward the region of higher REDV density, reaching 86% directionality in the middle of the gradient. The migration rate of ECs was also significantly enhanced twofold compared with that on tissue culture polystyrene (TCPS). In contrast, the gradient significantly weakened the adhesion of SMCs to 25% of that on TCPS but had no obvious impact on the migration rate and directionality. Successful modulation of the selective adhesion and directional migration of ECs over SMCs on biodegradable polymers serves as an important step toward practical applications for guided tissue regeneration.
研究领域:
生物材料学
·
细胞生物学
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DOI
PubMed
高影响因子
Food Structure-Netherlands
·
2025
·
IF 5.9
Pai Peng,Xiaolong Wang,Xinyu Zhang,Xinrui Ren,Xinzhong Hu
To study the effect of gluten strength on starch structure and digestibility during noodle processing, two gluten samples containing HMW-GSs N/17 + 18/5 + 10 and N/17 + 18/2 + 12, respectively, were separated from wheat near-isogenic lines and combined with identical wheat starch to prepare two recombinant noodles. The structural changes in protein, starch, and their interactions during noodle cooking and retrogradation were investigated. The results indicated that the noodles with 5 + 10 subunits exhibited better cooking and textural characteristics compared to 2 + 12. Cooking and retrogradation led to protein aggregation, with the 5 + 10 noodles displaying higher levels of SDS-unextractable polymeric proteins characterized by increased disulfide bonds, hydrophobic interactions, and β-sheets. Meanwhile, the double helices within starch were disrupted, producing more single helices rich in inclusion complexes. The dense and extensive gluten matrix in 5 + 10 noodles inhibited the destruction of starch granules during cooking, and promoted starch recrystallization and the formation of starch-protein complexes, which contributed to a higher RS content (29.13 %). In contrast, the 2 + 12 noodle exhibited greater release of short-chain starches and monomeric proteins during cooking, leading to a disordered structure that impeded starch recrystallization and improved starch digestibility. The findings support improving noodle quality and starch digestion resistance through the incorporation of suitable HMW-GSs.
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高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 9
·1-18
·
IF 2.6
Xu Wenyi,Guan Yinghua,Tian Liping,Fu Yu,Zhang Yinghui,Liu Yanhong,Hu Yanfei,Zhao Bowen,Wu Chongming
The global prevalence of type 2 diabetes mellitus (T2DM) is currently surging, posing significant health and socioeconomic burdens. Probiotics have emerged as promising interventions due to their safety and potential metabolic benefits. Gut microbiota plays a critical role in the pathophysiology of metabolic diseases in humans, offering valuable insights for therapeutic strategies such as probiotics. Here, aiming to identify novel probiotics from the human gut microbes to combat T2DM, a total of 203 human intestinal bacterial strains were screened in vitro to evaluate their enzymatic inhibition of α-glucosidase and α-amylase, in which Lacticaseibacillus paracasei Glu-07 (= QH-142 = CGMCC 23796) exhibited the highest inhibitory efficacy (81.05% and 72.56% for α-glucosidase and α-amylase, respectively). In HepG2 cells, L. paracasei Glu-07 elevated glucose uptake and consumption by 22.4% and 85.8%. Oral administration of strain Glu-07 in diabetic mice significantly improved hyperglycemia, hyperlipidemia, and restored the structure and composition of gut microbiota toward a healthier profile. Mechanistically, strain Glu-07 activated the AMPK-AKT1-GSK3β/FOXO1 signaling pathways by modulating the expression of key genes, enhanced glycolysis and fatty acid ꞵ-oxidation, and inhibited gluconeogenesis and lipogenesis. It also stimulated GLP-1 secretion, potentially via triggering the GPR41/43-GLP-1 axis, and increased the abundance of beneficial gut microbes, such as Akkermansia muciniphila (~ threefold). The dual regulations synergistically support strain Glu-07 as a promising probiotic candidate for T2DM management.
研究领域:
微生物学
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糖尿病研究
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分子生物学
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药理学
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营养科学
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代谢性疾病
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胃肠病学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xiaoyu Zhao,Chenglong Xue,Yu Liu,Tao Wang,Qi Shuai
The treatment of diabetic wounds remains a challenge due to the interaction between hyperglycemia, immune imbalance and bacterial infection. Herein, we developed a Glu and GSH double-unlocked immunomodulatory nanocascade reactor to accelerate diabetic wounds healing which dynamically manages the wound microenvironment by controlling blood glucose levels, restoring immune regulation and eliminating bacteria. On the one hand, ultra-small gold nanoparticles with GOx activity can catalyse glucose at the diabetic wound site, which can be further enhanced by photothermal effects, thus destroying bacterial biofilm structures, reversing wounds pH, and reducing bacterial reproduction. On the other hand, the sustained controlled release of NO at specific sites was achieved by depleting over-expressed GSH in the wounds to restore normal immune regulation, which not only promotes M2 macrophage polarisation and expressions of anti-inflammatory factors such as IL-10 and TGF-β1, but also produces VEGF and CD31 via the PI3K/AKT signalling pathway. Especially, the chemical resonance energy transfer (CRET) strategy was first applied to diabetic wounds treatment, achieving chemical photodynamic therapy (PDT) while reducing the secondary tissue damage caused by catalytically-generated H 2 O 2 . In summary, the nanocascade reactor provides a new reference for bacterialy-infected diabetic wounds treatment mode by reducing blood glucose and restoring immune regulation in parallel.
研究领域:
纳米技术
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免疫学
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伤口愈合
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糖尿病
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细菌学
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生物化学
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药学
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DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 76
·期 12
·4210-4221
·
IF 3.75
Qiuyan Zhu,Yang Yang,Zhiting Lao,Yingying Zhong,Kun Zhang,Suqing Zhao
BACKGROUND Pyrethroids, a class of insecticides, that act on the nervous system of insects. Frequent consumption of foods with pyrethroid residues increase the risk of developmental and neurological diseases in humans. Assessing the toxicity of novel synthetic pyrethroids to mammals is also critical to the development of agrochemicals. RESULTS Using mice as models, the acute and chronic toxicity of deltamethrin, permethrin, dihaloacetylated pyrethroids to mammals was researched by gavage administration. Acute toxicity assessment displayed that the median lethal dose (LD 50 ) of deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 tested were greater than 500 mg/kg of weight. Furthermore, chronic toxicity assessment demonstrated that deltamethrin, permethrin caused epidermal damage near the genitals, while dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 showed no relevant symptoms. However, both the acute and chronic toxicity assessment suggested that pyrethroids exposure induced mice loss weight. Additionally, the elevated plus maze (EPM) test showed that pyrethroids caused anxiety-like behaviors and no motor defects in Kunming mice. Beside, during the sucrose preference test (SPT), 60-day pyrethroids exposure increased excitatory behaviors in mice. However, the neurochemical studies displayed that pyrethroids exposure increased the total amount of glutamate (Gln), glutamine (Glu) and γ -aminobutyric acid (GABA) in the mice's blood. CONCLUSION Pyrethroids exposure induced weight loss in mice, although the acute oral toxicity of deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 was low. However, regarding chronic toxicity, deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 induced anxiety-like behaviors, excitatory behaviors, Gln-Glu-GABA circulatory dysfunction in blood. Particularly, deltamethrin, though permethrin also had reproductive toxicity. © 2020 Society of Chemical Industry
研究领域:
毒理学
·
神经科学
·
农业化学
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PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2023
·
IF 3.6
Qinming Xu,Hui Chen,Junming Zhu,Xing Li,Jingang Yang,Xiaocui Deng,Qingmei Yuan
In this study, the PEG-Glu-Lys-Glu copolymer drug delivery system (GO/PEG-Glu-Lys-Glu) is prepared using glutamate-lysine-glutamate (Glu-Lys-Glu) modified polyethylene glycol (PEG) and connected graphene oxide nanosheets (GO). The multiple carboxyl groups of Glu-Lys-Glu and π–π interactions of GO can increase drug loading rate, and the fluorescence characteristics of GO could monitor the distribution of drug-loading systems in cells and the uptake of cells without the need for external dyes. Paclitaxel (PTX) is loaded via reduction-responsive disulfide bonds as a model medicine to examine the drug delivery potential of GO/PEG-Glu-Lys-Glu. The results showed that the drug loading content of PEG-Glu-Lys-Glu and GO/PEG-Glu-Lys-Glu to PTX is 7.11% and 8.97%, and the loading efficiency is 71.05% and 89.68%, respectively. It’s speculated that the π–π interaction between GO and PTX improved the drug-loading capacity and efficiency of GO/PEG-Glu-Lys-Glu. In vitro, in a simulated drug release test, at 48 h, the release of PTX was 85.51% at pH 5.0, 65.12% and 38.32% at pH 6.5 and 7.4, respectively. The cytotoxicity assay results showed that GO/PEG-Glu-Lys-Glu cell inhibition rate to MCF-7 cells was 7.36% at 72 h. The cell inhibition rate of GO/PEG-Glu-Lys-Glu/PTX system at 72 h was 92%, equivalent to free PTX. Therefore, the GO/PEG-Glu-Lys-Glu drug delivery system has the characteristics of good biocompatibility and sustainable release of PTX, which is expected to be applied in the field of tumor therapy.
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PubMed
高影响因子
CHIRALITY
·
2020
·Vol. 32
·期 8
·1062-1071
·
IF 2.171
Min Liu,Zhong-Qian Liu,Hai Zhu,Chao-Hong He,Ke-Jun Wu
In this paper, a novel l -glutamate based immobilized chiral ionic liquid (SBA-IL (Glu)) was prepared by chemical bonding method and applied as a solid sorbent for chiral separation of amlodipine. The performance of SBA-IL (Glu) was investigated for the absorption of ( S )-amlodipine and separation of amlodipine enantiomer. The static experiment showed that equilibrium adsorption was achieved within 80 minutes, and the saturation adsorptions capacity was 12 mg/g. The complex was then packed in a glass chromatographic column for the separation of amlodipine and the enantiomeric excess (%ee) of ( S )-amlodipine reached 24.67%. The immobilized ionic liquids exhibit good reusability, and the separation efficiency remains 18.24% after reused five times, which allows potential scale-up for the chiral separation of amlodipine.
研究领域:
药物化学
·
分析化学
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DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2022
·Vol. 99
·期 1
·68-77
·
IF 3.521
Xianhu Wei,Qihui Gu,Ying Feng,Youxiong Zhang,Ying Li,Shuhong Zhang,Jumei Zhang,Shi Wu,Xiaojuan Yang,Qinghua Ye,Yu Ding,Juan Wang,Moutong Chen,Qingping Wu
A new, simple-to-synthesize and sensitive turn-on fluorogenic substrate ( CFMU-Glu ) for β-glucosidase activity was developed. This probe was based on a 7-hydroxycoumarin derivative ( CFMU ) that could emit green fluorescence and had the low p K a value of 5.61 ± 0.01. CFMU-Glu could be used for sensitive monitoring of the almond βGLU and Enterococcus faecalis ( E. faecalis ) at the optimal pHs of 6.50 and 7.00, respectively. Moreover, a new sensitive and selective fluorogenic broth (PBF-B) for E. faecalis , utilizing CFMU-Glu and polymyxin B, was also developed. Polymyxin B was discovered to can significantly improve the detection selectivity and signal intensity. The proposed 4-four method using PBF-B and a microcentrifuge tube could provide fluorogenic detection limits of 5.01 × 10 4 and 1.0 × 10 5 CFU mL −1 by fluorescence microplate reader and naked eye, respectively; it could also provide a turn-on chromogenic detection limit of 1.0 × 10 6 CFU mL −1 by naked eye. The proposed method could detect 8 CFU mL −1 of E. faecalis in drinking water, Liangcha (herbal tea) and milk samples within 10 h, without pre-enrichment.
研究领域:
微生物学
·
分析化学
·
食品科学
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DOI
PubMed
高影响因子
LANGMUIR
·
2016
·
IF 3.993
Genxing Zhu,Shasha Yao,Halei Zhai,Zhaoming Liu,Yaling Li,Haihua Pan,Ruikang Tang
Aggregation-based crystal growth is distinct from the classical understanding of solution crystallization. In this study, we reveal that N-stearoyl-l-glutamic acid (C18-Glu, an amphiphile that mimics a biomineralization-relevant biomolecule) can switch calcite crystallization from a classical ion-by-ion growth to a non-classical particle-by-particle pathway, which combines the classical and non-classical crystallization in one system. This growth mechanism change is controlled by the concentration ratio of [C18-Glu]/[Ca2+] in solution. The high [C18-Glu]/[Ca2+] can stabilize precursor nanoparticles to provide building blocks for aggregation-based crystallization, in which the interaction between C18-Glu and the nanoprecursor phase rather than that of C18-Glu on calcite steps is highlighted. Our finding emphasizes the enrollment of organic additives on metastable nano building blocks, which provides an alternative understanding about organic control in inorganic crystallization.
研究领域:
材料科学
·
生物矿化
·
结晶学
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DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Yuan Fang-Fang,Wang Pei,Han Xiao-Jie,Qin Ting-Ting,Lu Xin,Bai Hai-Jiao
The microreactor with two types of immobilized enzymes, exhibiting excellent orthogonal performance, represents an effective approach to counteract the reduced digestion efficiency resulting from the absence of a single enzyme cleavage site, thereby impacting protein identification. In this study, we developed a hydrophilic dual-enzyme microreactor characterized by rapid mass transfer and superior enzymatic activity. Initially, we selected KIT-6 molecular sieve as the carrier for the dual-IMER due to its three-dimensional network pore structure. Modification involved co-deposition of polyethyleneimine (PEI) and acrylamide (AM) as amine donors, along with dopamine to enhance material hydrophilicity. Remaining amino and double bond functional groups facilitated stepwise immobilization of trypsin and Glu-C. Digestion times for bovine serum albumin (BSA) and bovine hemoglobin (BHb) on the dual-IMER were significantly reduced compared to solution-based digestion (1 min vs. 36 h), resulting in improved sequence coverage (91.30% vs. 82.7% for BSA; 90.24% vs. 89.20% for BHb). Additionally, the dual-IMER demonstrated excellent durability, retaining 96.08% relative activity after 29 reuse cycles. Enhanced protein digestion efficiency can be attributed to several factors: (1) KIT-6’s large specific surface area, enabling higher enzyme loading capacity; (2) Its three-dimensional network pore structure, facilitating faster mass transfer and substance diffusion; (3) Orthogonality of trypsin and Glu-C enzyme cleavage sites; (4) The spatial effect introduced by the chain structure of PEI and glutaraldehyde’s spacing arm, reducing spatial hindrance and enhancing enzyme–substrate interactions; (5) Mild and stable enzyme immobilization. The KIT-6-based dual-IMER offers a promising technical tool for protein digestion, while the PDA/PEI/AM-KIT-6 platform holds potential for immobilizing other proteins or active substances.
研究领域:
生物化学
·
蛋白质组学
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DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2026
·
IF 5.2
Xinrui Yan,Linfeng Ma,Jing Ren,Binyan Chen,Wenxuan Song,Yu Dong,Xiaojiao Li,Yingjie Guo
Valproic acid (VPA), a widely used antiepileptic drug, often induces hepatic steatosis during long-term treatment, though the underlying mechanisms remain incompletely understood. In our study, epileptic patients with abnormal liver function exhibited significantly elevated levels of homocysteine (Hcy) and glutamate (Glu) compared to those with normal liver function. Moreover, Hcy and Glu levels were positively correlated with markers of lipid accumulation and oxidative stress. Similar elevations in Hcy and Glu were observed in VPA-treated mice and hepatocyte models (HepG2, AML12), suggesting that disrupted Hcy/Glu homeostasis may be a hallmark of VPA-induced hepatic steatosis. Further in vitro experiments demonstrated that treatment with either Hcy or Glu not only increased lipid accumulation but also triggered ferroptosis, as indicated by Fe 2+ overload, suppression of the GSH-GPX4 axis, and ACSL4-mediated lipid peroxidation. These effects were attenuated by the ferroptosis inhibitor ferrostatin-1 (Fer-1), confirming the contribution of ferroptosis to Hcy/Glu-induced hepatotoxicity. Additional investigations revealed that VPA promotes Hcy accumulation by downregulating its catabolic enzymes (MTR, CBS, CTH), while enhancing Glu accumulation through upregulation of its synthetic enzymes (ALDH4A1, GLS) and repression of the catabolic enzyme GLUL, both in vivo and in vitro . The alterations in MTR, CBS, ALDH4A1, and GLS levels were shown to depend on HDAC1, as demonstrated using HDAC1 knockdown and overexpression systems in HepG2 cells. In summary, VPA disrupts Hcy/Glu homeostasis via HDAC1, leading to ferroptosis and subsequent hepatic steatosis. These findings highlight the HDAC1-Hcy/Glu-ferroptosis axis as a promising therapeutic target for mitigating VPA-induced hepatotoxicity.
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PubMed
高影响因子
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