ANALYTICA CHIMICA ACTA
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2026
·
IF 6.1
Xinyi Wang,Yuying Lin,Rubing Xiong,Jing Liu,Yue Liu,Zijie Li,Yongmei Chen,Yong Xia,Zhiqi Li,Lei Mou,Meiling Liu
Background Continuous lactate monitoring provides valuable information on metabolic dynamics during exercise and recovery. Enzymatic lactate sensors commonly use lactate oxidase, whose catalytic response depends on local oxygen availability. As physiological oxygen levels fluctuate, a calibration model established under one oxygen condition may generate substantial measurement bias under another condition. Existing wearable lactate sensors generally use a single calibration model and therefore cannot compensate for oxygen-dependent changes in sensor response. The key problem addressed in this study is the lack of an oxygen-adaptive calibration strategy for accurate continuous lactate monitoring in complex physiological microenvironments. Results We developed a wearable electrochemical platform based on a carbon nanotube/Prussian blue/lactate oxidase interface and integrated it with a custom portable microcircuit. The system classified currents less negative than −0.8 μA as hypoxic and those more negative than −0.8 μA as aerobic, and applied the corresponding calibration model in real time. The sensor showed linear responses from 2 to 12 mM lactate, with sensitivities of 0.2247 μA/mM under aerobic conditions and 0.01256 μA/mM under hypoxic conditions. Plasma measurements agreed closely with a commercial biochemical analyzer (r = 0.9860). In rats, sensor readings correlated strongly with blood lactate assay results during both aerobic (r = 0.9766) and hypoxic (r = 0.9316) phases. The oxygen-adaptive strategy achieved a mean absolute relative difference of 4.86%, compared with 59.72% and 1028% for the single aerobic and hypoxic calibration models, respectively. Significance and novelty This study introduces an oxygen-adaptive calibration strategy that converts oxygen-dependent signal variation from a source of error into a basis for real-time model selection. By combining oxygen-condition classification with condition-matched calibration, the platform markedly improves lactate measurement accuracy across aerobic and hypoxic conditions. This approach provides a practical framework for continuous biosensing in physiologically variable microenvironments.
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Stem Cell Reports
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2026
·
IF 5.6
Zhishuai Zhang,Yuchan Mai,Jun Tang,Jiaying Ning,Yanjiao Qin,Jiaming Gu,Zhaozong Cao,Jian Liu,Tiancheng Zhou,Junwei Wang,Jeane L. Pan,Jiaheng Chen,Yanling Zhu,Guangjin Pan
Natural Killer (NK) cells generally exhibit dysfunction in tumor microenvironment (TME), significantly limiting their efficacy in antitumor therapy. Tumor cells display enhanced glycolysis, leading to lactic acid (LA) secretion and accumulation in the TME. Using human-induced pluripotent stem cell (hiPSC)-derived NK cells (iNKs) as a model, we demonstrate that LA induces substantial iNK dysfunction, including reduced survival, impaired IFN-γ secretion, and diminished tumor-killing capacity due to severely compromised mitochondrial function. To overcome LA-induced dysfunction, we knocked in an expression cassette for lactate dehydrogenase B (LDHB) into hiPSCs (LDHB-hiPSCs), enabling conversion of cellular lactate to pyruvate. iNKs derived from LDHB-hiPSCs (LDHB-iNKs) largely resisted LA-induced dysfunction, exhibiting enhanced cytotoxicity and improved survival in high-LA tumor tissues. Importantly, LDHB-iNKs show superior suppression of solid tumor formation in vivo . Our study provides a novel strategy to enhance NK cell therapy against solid tumors by reshaping the metabolic pathways.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yuzhu Zhao,Huawei Gu,Rodrigo Ledesma-Amaro,Ling He,Shuwen Liu,Kan Shi
β-Glucosidase (BGL) plays a key role in wine aroma enhancement by hydrolyzing glycosidic precursors; however, its application is limited by instability under harsh winemaking conditions. To address this, we developed an integrated biocatalytic system by immobilizing an acid-tolerant BGL onto functionalized magnetic oak chips (NFOak) via oriented Ni 2+ -histidine coordination. Comprehensive characterization confirmed the fabrication of a porous, magnetic cellulose scaffold. The immobilized BGL exhibited significantly enhanced stability against winemaking stresses (low pH, high ethanol) compared to the free enzyme. Furthermore, magnetic actuation of the biocomposite improved its hydrolytic performance, likely attributed to enhanced mass transfer. When applied to Chardonnay and Marselan wines, the magnetically actuated system effectively hydrolyzed glycosidic precursors, markedly increasing the concentrations of key volatile compounds-such as linalool and ethyl benzoate associated with fruity and floral aroma profiles. The treatment also induced subtle modulations in wine color and phenolic composition. Safety assessments confirmed no nickel leaching and oenologically acceptable iron levels. This work provides a sustainable and efficient strategy for controllable aroma modulation, combining the oenological benefits of a natural oak carrier with the operational controllability of magnetic actuation.
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ACS Omega
·
2026
·
IF 4.3
Yutong Feng,Hui Sun,Xinyu Liu,Huiping Wu,Gaoyuan Chen,Afang Gao,Shijie Wang,Jiahui Zhou,Bin Yu
Flexible electrochemical sensors based on noninvasive materials can continuously monitor blood glucose levels in sweat, making them ideal for personal diabetes management intelligent devices. It is necessary to construct sweat glucose electrochemical sensors that have the ability to both adjust to human skin and reliably monitor. We first synthesized nickel/cobalt metal–organic framework material (Ni/Co-MOF) nanosheets via a solvent-thermal approach. Then, an oil-based polyurethane (OPU)/polypropylene spun-bonded nonwoven fabric (PPSF) was obtained by coating the surface of PPSF. Silver/silver chloride (Ag/AgCl) paste and conductive carbon (C) paste were printed layer-by-layer on the surface of the OPU/PPSF by screen printing, followed by the immobilization of Ni–Co/MOF nanosheets on the working area using a Nafion solution to form a Ni/Co-MOF@OPU/PPSF flexible glucose sensor for continuous monitoring of glucose in sweat. This Ni/Co-MOF@OPU/PPSF flexible glucose sensor retained the high electrocatalytic activity of Ni/Co-MOF for glucose oxidation and exhibited high sensitivity (159.9 μA/mM·cm2), low limit of detection (LOD) (2 μM), and a wide linearly glucose-monitoring range (10 μM–1 mM). Compared to other nonenzymatic sensors, our Ni/Co-MOF@OPU/PPSF flexible glucose sensor has better immunity than other substances present in sweat. Meanwhile, the Ni/Co-MOF@PPSF flexible glucose sensor not only monitors the glucose in sweat at different body temperatures but also maintains good mechanical flexibility and stable electrochemical properties under bending conditions. It is suggested that our flexible glucose sensor can be used for continuous monitoring of glucose in human sweat. Overall, our glucose sensor can be used for the continuous monitoring of glucose in human sweat.
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ACS Sensors
·
2026
·
IF 9.1
Shaorui Qi,Lei Sun,Fansheng Meng,Guimei Shi,Yunlong Sun,Bo Jiang,Junbo Li,Yukihiro Ozaki,Wei Ji
Abnormal lactic acid (LA) enantiomeric excess (ee) in biofluids is closely associated with various intestinal diseases. Here, we report a chiral-label-free nonplasmonic surface-enhanced Raman scattering (SERS) platform using a ZIF-8/ZnS heterojunction that enables direct quantification of LA enantiomers and their ee values in raw human urine through a charge-transfer-driven chiral recognition mechanism. Our findings indicate that an enantioselective hydrogen bonding between 4-mercaptopyridine (4-MPy) probes and LA enantiomers induces differential charge-transfer effects within the ZIF-8/ZnS@4-MPy system, evidenced by the selective enhancement of specific vibrational modes in the SERS spectra of 4-MPy and enantiomer-specific changes in the fluorescence lifetime of ZnS. This innovative system integrates signal amplification and stereoselectivity without the use of noble metals or chiral modifiers, overcoming critical limitations associated with the complexity of preparing chiral plasmonic substrates. The platform achieves ultrasensitive detection limits (10 nM), linear response to ee values (R2 = 0.98), high measurement precision (RSD < 8.28%, n = 20), long-term stability (28-day), and clinical-grade accuracy against enzymatic assays (RMSEP = 1.97). This work presents an efficient, noninvasive method for the analysis of chiral metabolites in urine, while establishing a novel direction for plasmon-free SERS chiral sensing.
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Advanced Sustainable Systems
·
2026
·Vol. 10
·期 2
·e00001
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IF 5.6
Aohui Ping,Mei Li,Lu Ding,Meijuan Ding,Ziyu Li,Jingyi Nie,Xianglong Lyu,Chaoqi Ding,Youji Li,Zhiliang Jin
In order to investigate the influence of the ohmic junction formed at the interface between the bimetallic sulfide NiMnS and maple-leaf CdS on photocatalytic hydrogen evolution performance, this work employed a simple hydrothermal method to synthesise maple-leaf CdS and granular NiMnS. The optimized composite material (15NiMnS/CdS) achieved an exceptional hydrogen evolution rate of 34.83 mmol·g −1 ·h −1 under visible light illumination within 5 h, surpassing the rates of CdS alone and NiMnS alone by 5.44-fold and 530.70-fold, respectively. The superior photocatalytic hydrogen evolution performance originates from the fact that the Fermi level of the metal-like catalyst NiMnS is higher than that of the semiconductor catalyst CdS. This allows the two to form an ohmic junction after contact, creating a strong electronic coupling interface. Electron enrichment occurs at CdS, which inhibits electron–hole recombination and enables electrons to engage in the photocatalytic process efficiently, thus improving its photocatalytic hydrogen evolution ability. The incorporation of NiMnS co-catalyst increased the CdS charge separation efficiency from 18.67% to 68.16%, thereby improving the catalyst's reaction kinetics and enhancing the photocatalytic hydrogen generation rate. This study presents an example of engineering transition-metal sulfide-based Ohmic-contact structures and enhancing photocatalytic hydrogen evolution performance through strong electronic coupling.
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BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Xue Wang,Lin He,Zihan Li,Zongqian Hu
In modern medicine, glycemic control is critical for the health of individuals with diabetes. Over decades of care, patients with diabetes have relied on glucose meters for monitoring and on insulin or adjunct hypoglycemic agents for glycemic control. Frequent blood sampling and injections increase infection risk, restrict patient mobility, and generate substantial single-use medical waste. We present a simple, user-friendly closed-loop blood-glucose management system. The system integrates a highly sensitive glucose sensor and a controllable drug-release module within a dual-mode microneedle array, coupled with a miniature electrochemical workstation, thereby enabling dynamic glucose monitoring, precise drug delivery, and stable glycemic control with minimal patient intervention. The system's design demonstrates potential for achieving closed-loop glucose management. Preliminary proof-of-concept studies in diabetic mouse models yielded positive trends in relevant data. This work establishes an engineering framework integrating dual-mode microneedle devices with portable bioelectronic devices, providing a technical foundation for future mid-to-long-term closed-loop glucose management.
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Cell Reports
·
2026
·Vol. 45
·期 3
·
IF 7.7
Chao Li,Han Zhao,Jingjing Wang,Chenfei Liu,Shiqing He,Jingchao Zhou,Liangyu Li,Yiyao Liu,Qingping Wang,Xiaojing Shen,Junjie Wu,Guangquan Chen,Yu Wang,Liang Yang,Bin Du
Tumor-resident microbiota are identified as a critical modulator in the carcinogenesis and progression of cervical malignancies. However, the mechanisms linking these microbiota to cervical cancer remain poorly understood. In this study, we find that patients with cervical cancer exhibit increased microbiota diversity, characterized by eight distinct bacterial species that distinguish them from control subjects. Intratumoral or intravenous administration of an isolated strain, Gordonia polyisoprenivorans GP-2, derived from tumor-resident microbiota, significantly promotes the growth and metastasis of cervical cancer in mice. Further investigation reveals that GP-2 metabolizes host-derived choline to produce the bacterial metabolite lysophosphatidylcholine (lysoPC). We propose and validate the biosynthetic pathway for lysoPC in strain GP-2 and demonstrate that lysoPC activates the classical tumorigenic genes c-Jun / c-Fos using cervical tumor organoids derived from patients. These findings elucidate the mechanisms underlying microbiota-host crosstalk in cervical cancer and highlight a potential therapeutic target for intervening in tumor-resident bacteria.
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CHEMICAL ENGINEERING JOURNAL
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2026
·
IF 12.5
Tingting Yu,Peng Song,Ruixue Wang,Ziwen Chen,Liang Zhao,Ming Wang,Jianying Wang,Tao Mei,Xianbao Wang,Jinhua Li
Colorectal cancer (CRC) is one of the malignant tumors with high morbidity and mortality rates worldwide. Conventional screening methods, such as endoscopy and fecal testing, are invasive and have low compliance, highlighting the urgent need for efficient, non-invasive diagnostics. Circulating small RNAs, including the sequence annotated as PIWI-interacting RNA-54265 (piR-54265), have been reported to be closely associated with CRC and represent promising serum biomarkers in liquid biopsy research. This study aims to develop an ultrasensitive, label-free solution-gated graphene transistor (SGGT) based biosensor to detect piR-54265 in serum samples, addressing limitations of current CRC biomarker detection. The Polyadenine 15 -thymine 5 (PolyA 15 T 5 -DNA) probe was designed with an L-shaped configuration, constructing a sensing interface. This configuration is proposed to facilitate a favorable probe conformation for nucleic acid recognition at the gate interface, thereby contributing to the overall sensing performance of the biosensor. The biosensor achieves a remarkable limit of detection (LOD) of 8.56 × 10 −19 M, with a linear detection range from 1 aM to 10 nM, and excellent selectivity for piR-54265. Clinical serum samples analyzed under diluted conditions demonstrate clear differentiation between CRC patients and non-cancer controls, with receiver operating characteristic (ROC) analysis further confirming its exceptional diagnostic performance and achieving an area under the curve (AUC) of 1.0. These results establish a SGGT-based sensing platform for CRC-associated RNA detection and highlight its potential for non-invasive biomarker detection and future diagnostic applications.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Bo Wang,Feng Zhao,Zheng Zhou,Ji Wang,Lei Qin,Xuhui Huang
Studied hazardous compound changes in the thermal processing of 7 animal foods. • Hazards: acrylamide, 5-hydroxymethylfurfural, 13 heterocyclic aromatic amines. • AA, 5-HMF, and 13 HAAs in all animal foods increased with roasting temperature. • Low moisture and high carbs promoted AA; high protein favored HAAs. • Amino acids and dipeptides had negative correlations with hazardous compounds. The thermal processing of food is often accompanied by changes in composition and the generation of hazardous compounds. However, the unclear mechanisms linking food composition to related hazardous compounds hinder the advancement of inhibition technologies for hazardous compounds formed during thermal processing. In this paper, the mechanisms of 15 kinds of associated hazardous compounds produced by 7 different animal-derived food samples during the roasting process were investigated. When the roasting temperature was 260 °C, mutton exhibited the highest acrylamide content at 0.11 μg/g, chicken had the highest HAAs (heterocyclic aromatic amines) content at 0.17 μg/g, and Antarctic krill showed the highest 5-hydroxymethylfurfural content at 0.72 μg/g. notably, the compounds closely related to the production of associated hazards during thermal processing include amino acids, small dipeptides, and certain carbohydrate compounds. The results of this study have positive significance in improving food safety and optimizing production processes. Download: Download high-res image (136KB) Download: Download full-size image
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npj Flexible Electronics
·
2026
·
IF 15.4
Lin Kanglong,Yang Baili,Yan Guizhi,Li Changhao,Zhao Guoxu,Zou Yang,Li Zhou,Wang Dong
Chronic diabetic wounds present a significant clinical challenge due to antibiotic abuse and limited understanding of the wound microenvironment. Real-time monitoring of key biomarkers in the wound microenvironment can provide critical insights into wound status and guide therapeutic interventions. In this study, we developed an intelligent wound patch by integrating a near-infrared (NIR)-responsive photothermal antibiotic-releasing hydrogel (PPQTA) with a multichannel electrochemical biosensor array, which takes sensor monitoring-guided precise drug administration as its core. The sensing module can continuously monitor the key biomarkers in the wound in situ with high sensitivity and strong anti-interference capability within the physiological range (pH: 4–9; uric acid: 0–700 μM; glucose: 5–30 mM), providing a quantitative basis for drug release. The PPQTA hydrogel can release drugs on demand via NIR irradiation based on the sensing results, achieving 100% antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Experiments in a diabetic rat wound model confirmed that this patch can monitor the dynamic changes of biomarkers in situ and guide drug administration within 7 days. After terminating drug administration on day 4, the bacteria in the wound were effectively eliminated on day 5, and the wound healing process was significantly promoted. By combining real-time diagnostics with responsive therapy, this patch offers a closed-loop strategy for personalized management of diabetic chronic wounds.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 13
·6308
·
IF 4.9
Yongmei Wu,Wenqian Song,Xuxian Wu,Jing He,Min Su,Rong Hu,Youbo Zhao
Autoimmune hepatitis (AIH) is linked to an increased risk of hepatocellular carcinoma (HCC). However, the precise connection between the two remains unclear. GPR81, a G-protein-coupled receptor located on the membranes of various cell types, plays a role in numerous physiological processes. We established an AIH animal model and activated GPR81 using the agonist 3,5-dihydroxybenzoic acid (3,5-DHBA). Additionally, the effect of GPR81 inhibition on tumor and immune cell dynamics was examined using the HepG2, Hep3B, and Hepa1-6 cell lines with the antagonist 3-hydroxybutyric acid (3-OBA). Our results demonstrated that 3,5-DHBA treatment reduced T cell and pro-inflammatory cytokine secretion, while MDSC secretion increased, inhibiting Concanavalin A (Con A)-induced AIH. The inhibition of GPR81 by 3-OBA suppressed HCC cell proliferation and invasion, reduced tumor volume and weight, and downregulated PD-L1 expression. Furthermore, CTL and DC activity in the spleen and tumors increased, while MDSC activity decreased. This study confirms that GPR81 plays an important role in both inflammation and tumorigenesis, suggesting that GPR81 may serve as a bridge in the transformation of inflammation into cancer. Modulating GPR81 activity may provide a novel therapeutic strategy for hepatitis and cancer.
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