INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Jie Bai,Yongchun Huang,Yuling Zhao,Lirong Zhang,Fuli Zhu,Yixizhuoma,Yuxin Weng,Shoude Zhang
The structural characterization of polysaccharides requires precise determination of their monosaccharide composition, including the differentiation of D- and L-configurations. Current analytical methods can identify monosaccharide types but often fail to distinguish between enantiomeric configurations, posing a significant challenge for accurate polysaccharide structural elucidation. This study introduces a robust, accurate, and reproducible ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method to address this limitation. By utilizing ( S )-(−)-1-phenylethylamine (S-PEA) as a chiral derivatizing reagent, monosaccharide enantiomers are converted into diastereomers through the introduction of a new chiral center. This approach enables the simultaneous determination of D/L configurations for aldoses, amino sugars, alditols, and uronic acids, achieving excellent chromatographic baseline separation. Method validation demonstrated excellent linearity (R 2 > 0.9900) within the range of 2.5–160 μg/mL. The limits of detection (LOD) and quantification (LOQ) ranged from 0.04 to 0.11 μg/mL and 0.13–0.35 μg/mL, respectively, indicating high sensitivity. Accuracy was confirmed with recovery rates between 97.23% and 100.67%, while intra- and inter-day precision RSDs were below 3.54%. The method was successfully applied to characterize the monosaccharide composition of six plant-derived polysaccharides, demonstrating its efficacy in identifying both structural types and absolute configurations. These findings establish the proposed method as a valuable tool for advancing the comprehensive structural analysis of polysaccharides.
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Food Bioscience
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2026
·
IF 6.2
Xiangkun Du,Yasen Wang,Buqing Wang,Yu Shen,Shu Teng,Jiawen Bai,Zijie Li
Saccharomyces cerevisiae spores have emerged as a novel surface display host and have been successfully applied in the biosynthesis of useful products. To construct a dual-enzyme co-display and assembly system on the surface of S. cerevisiae spores, the fluorescent proteins were initially used as the model proteins to prove the feasibility. The result of the in vitro assembly experiment of L-arabinose isomerase from Bacillus coagulans ( Bc AI) and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans ( Gt MPI) confirmed that the assembled complex could significantly improve the biosynthetic efficiency of the rare sugar L-ribose. Subsequently, Bc AI and Gt MPI were co-displayed on the spore surface and further assembled via the SpyTag/SpyCatcher system to realize the biosynthesis of L-ribose. The maximum conversion rate of L-ribose reached approximately 16% with 10 g/L L-arabinose as the substrate. Nevertheless, the achieved L-ribose conversion rate of 16% is moderate compared with previously reported values. Even with this limitation, this study establishes a feasible spore-surface assembly strategy for multi-enzyme cascades, laying a technical foundation for the biosynthesis of high-value products.
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MOLECULES
·
2022
·Vol. 27
·期 9
·3027
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IF 4.927
Tingting Li,Haiqiong Ma,Hong Li,Hao Tang,Jinwen Huang,Shiying Wei,Qingxia Yuan,Xiaohuo Shi,Chenghai Gao,Shunli Mi,Longyan Zhao,Shengping Zhong,Yonghong Liu
Laminaria japonicais widely consumed as a key food and medicine. Polysaccharides are one of the most plentiful constituents of this marine plant. In this study, several polysaccharide fractions with different charge numbers were obtained. Their physicochemical properties and anticoagulant activities were determined by chemical and instrumental methods. The chemical analysis showed thatLaminaria japonicapolysaccharides (LJPs) and the purified fractions LJP0, LJP04, LJP06, and LJP08 mainly consisted of mannose, glucuronic acid, galactose, and fucose in different mole ratios. LJP04 and LJP06 also contained minor amounts of xylose. The polysaccharide fractions eluted by higher concentration of NaCl solutions showed higher contents of uronic acid and sulfate group. Biological activity assays showed that LJPs LJP06 and LJP08 could obviously prolong the activated partial thromboplastin time (APTT), indicating that they had strong anticoagulant activity. Furthermore, we found that LJP06 exerted this activity by inhibiting intrinsic factor Xase with higher selectivity than other fractions, which may have negligible bleeding risk. The sulfate group may play an important role in the anticoagulant activity. In addition, the carboxyl group and surface morphology of these fractions may affect their anticoagulant activities. The results provide information for applications ofL. japonicapolysaccharides, especially LJP06 as anticoagulants in functional foods and therapeutic agents.Keywords:Laminaria japonica;polysaccharides;physicochemical properties;anticoagulant activity
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Nature Communications
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2022
·Vol. 13
·期 1
·1-14
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IF 17.694
Arabi Maryam,Ostovan Abbas,Wang Yunqing,Mei Rongchao,Fu Longwen,Li Jinhua,Wang Xiaoyan,Chen Lingxin
Chiral discrimination is critical in environmental and life sciences. However, an ideal chiral discrimination strategy has not yet been developed because of the inevitable nonspecific binding entity of wrong enantiomers or insufficient intrinsic optical activities of chiral molecules. Here, we propose an “inspector” recognition mechanism (IRM), which is implemented on a chiral imprinted polydopamine (PDA) layer coated on surface-enhanced Raman scattering (SERS) tag layer. The IRM works based on the permeability change of the imprinted PDA after the chiral recognition and scrutiny of the permeability by an inspector molecule. Good enantiomer can specifically recognize and fully fill the chiral imprinted cavities, whereas the wrong cannot. Then a linear shape aminothiol molecule, as an inspector of the recognition status is introduced, which can only percolate through the vacant and nonspecifically occupied cavities, inducing the SERS signal to decrease. Accordingly, chirality information exclusively stems from good enantiomer specific binding, while nonspecific recognition of wrong enantiomer is curbed. The IRM benefits from sensitivity and versatility, enabling absolute discrimination of a wide variety of chiral molecules regardless of size, functional groups, polarities, optical activities, Raman scattering, and the number of chiral centers.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Shuo Shi,Guocheng Wang,Jie Liu,Sunan Liu,Qiuyu Xu,Xi Lan,Jianxing Feng,Jing Sun,Wentao Zhang,Jianlong Wang
Gentiana straminea Maxim. exhibits various biological activities. However, the purification and functions of polysaccharides in Gentiana straminea Maxim. have never been reported. Herein, by proposing a flexible 3D graphene-based decoloration column (3DD column), Gentiana straminea Maxim. polysaccharide (GMP) was high-throughput obtained and its anti-inflammatory activity was investigated. Benefiting from the large macroporous network of 3D NH 2 -graphene oxide hydrogel with selective adsorption towards pigments, the 3DD column exhibits high decoloration ratio (96.41%). In addition, the 3DD column provides superior practical functionality as compared to the traditional approaches, which are time-consuming and need toxic solvents, and exhibiting widespread-application for the purification of polysaccharide from other common plant species. More importantly, the decolored GMP as a natural product has promising anti-inflammatory activity on RAW264.7 cells without negative impact on cell viability . Overall, this work reveals a new functional polysaccharides and provides a flexible approach for polysaccharide decoloration, exhibiting a promising prospect for natural polysaccharides in practical application of pharmaceutical.
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Materials
·
2020
·Vol. 13
·期 3
·507
·
IF 3.057
Hui Wang,Meiyin Wang,Jining Shang,Yuanhang Ren,Bin Yue,Heyong He
In this work various amount of phosphomolybdic acid (PMo) were immobilized on amine functionalized SBA-15 and used as heterogeneous catalysts in the epimerization of glucose in aqueous solution. 13.3PMo/NH2-SBA-15 exhibited the best catalytic performance with a glucose conversion of 34.8% and mannose selectivity of 85.6% within two hours at 120 °C. The activation energy of 80.1 ± 0.1 kJ·mol−1was lower than that of 96 kJ·mol−1over the homogeneous H3PMo12O40catalyst. The catalytic activities of 13.3PMo/NH2-SBA-15 for the transformation of some other aldoses including mannose, arabinose and xylose were also investigated.Keywords:aldose epimerization;phosphomolybdic acid;amino-silanization;SBA-15
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2020
·Vol. 192
·期 3
·935-951
·
IF 2.277
Guo Zongren,Long Liangkun,Ding Shaojun
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn 2+ . Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
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FOOD CHEMISTRY
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2020
·
IF 6.306
Manli Guo,Jingtian Chi,Chi Zhang,Minglin Wang,Hui Liang,Juying Hou,Shiyun Ai,Xiangyang Li
In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.
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MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Baolei Fan,Guoliang Wei,Xiaofeng Gan,Tingting Li,Zengyi Qu,Sheng Xu,Chao Liu,Chunqi Qian
In this study, we reported the various contents of Polygonatum cyrtonema by extracting polysaccharides (PCPs) using steaming and shining procedures, thus providing scientific basis for the processing of Polygonatum cyrtonema and content analysis of polysaccharides. We established the characteristic fingerprint to identify and analyze polysaccharide component changes in distillated and processed Polygonatum cyrtonema by PMP precolumn derivatization and HPLC-MS/MS technology. We also classified two kinds of solutions by comparing the composition difference of 10 polysaccharide components in the samples with principal component and cluster analysis. There was a huge difference between them and seven monosaccharides. Processed PCPs mainly consisted of galactose along with mannose, followed by glucose. Meanwhile, we found that the results of cluster analysis and principal component analysis were the same. The polysaccharide components extracted from processed Polygonatum cyrtonema and distillate were mainly galactose and mannose. The results of cluster analysis and principal component analysis were the same. The distilled liquid can be divided into 5 categories, and the processed dry extract of Polygonatum cyrtonema can be divided into 4 categories. The changing trend of polysaccharide components of Polygonatum cyrtonema and distilled liquid was revealed during the multiple cycles of steaming and sun drying. The similarity and difference of polysaccharide components in the extracted liquid and the distilled liquid of each batch were shown.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Dongxue Cao,Enwei Wei,Zeyu Wang,Zhengyu Hu,Ling Qi,Hongli Zhou,Jingli Zhao
This paper aimed to elaborate the chemical characteristics of ginseng polysaccharide via chemical methods and instrumental analyses; additionally, the anti-glioma activity and immunostimulatory capacity were also evaluated via biological experiments. Ginseng polysaccharide by microwave-assisted extraction (MEGP) method was purified by spongiform diethylaminoethyl cellulose (DEAE) and further separated by DEAE-52 column chromatography to obtain four MEGP fractions (MEGP-0, MEGP-1, MEGP-2, MEGP-3). Utilizing Zeta potential analyzer and X-ray photoelectron spectroscopy (XPS), MEGPs were, for the first time, confirmed the valence state of C and O with the binding mode of elements, and all MEGP fractions contained triple-helix structure. MEGP-2 and MEGP-3 significantly reduced the cell growth rate of TJ905 and U87 , showing an inhibitory effect on glioma. Among four fractions, MEGP-2 markedly elevated the immunostimulatory capacity via the activation of MAPK signaling route, with an increasing the expression levels of p-p38, p-ERK and p-JNK. In summary, this paper laid a solid foundation for the development of ginseng, as a promising drug or tonic, in the fields of anti-glioma and immunoregulation.
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Polymers
·
2022
·Vol. 14
·期 21
·4636
·
IF 4.967
Zhengyu Hu,Jiaming Wang,Long Jin,Tieqiang Zong,Yuanqi Duan,Jinfeng Sun,Wei Zhou,Gao Li
This investigation focuses on the three novel polysaccharides fromCordyceps militarisand then discusses their characterization and anti-complementary activity. The three polysaccharides fromC. militaris(CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.Keywords:Cordyceps militaris;anti-complementary activity;polysaccharides;structure-activity relationship
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FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Zheng Qian,Long Si,Chen Zhi,Fu Jiaolong,Ju Xin,Li Liangzhi
Enzymatic preparation of rare sugars as an alternative to traditional sweeteners is an effective strategy to achieve a low-calorie healthy diet. Ribose-5-phosphate isomerase B (RpiB) is a key enzyme in the non-oxidative branch of the catalytic pentose phosphate pathway. Here, we investigated the potential of Curtobacterium flaccumfaciens ZXL1 ( C. flaccumfaciens ZXL1) derived RpiB (CfRpiB) in D-allose preparation. The optimal reaction conditions for recombinant CfRpiB were found experimentally to be pH 7.0, 55 °C, and no metal ions. The kinetic parameters K m , k cat , and catalytic efficiency k ca t / K m were 320 mM, 4769 s −1 , and 14.9 mM −1 s −1 respectively. The conversion of D-allulose by purified enzyme (1 g L −1 ) to D-allose was 13% within 1 h. In addition, homology modeling and molecular docking were used to predict the active site residues: Asp13, Asp14, Cys72, Gly73, Thr74, Gly77, Asn106, and Lys144.
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