Food Science and Human Wellness
·
2022
·
IF 8.022
Hongyuan Zhao,Xiaoyu Chen,Fanqiang Meng,Libang Zhou,Xinyi Pang,Zhaoxin Lu,Yingjian Lu
Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 10 9 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
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Food Bioscience
·
2024
·
IF 4.8
Chao Miao,Kai Dong,Yu Shen,Yilin Sun,Wenyan Li,Chaoxin Man,Yu Zhang,Qianyu Zhao,Yujun Jiang
Hyperuricemia is associated with various diseases. This study confirmed that intervention with Lacticaseibacillus rhamnosus JY027 ( L. rhamnosus JY027) reduced biochemical markers including uric acid , creatinine, and urea nitrogen in hyperuricemic mice. A combined analysis of gut microbiota , short-chain fatty acids (SCFAs), and metabolites based on the gut-kidney axis revealed that this bacterial intervention significantly enhanced the abundance of beneficial bacteria such as Bifidobacterium , Akkermansia , and Faecalibaculum and increased SCFA levels. The bacterial intervention also regulated the levels of guanosine , creatinine, arginine, choline , taurine and other differential metabolites in the kidneys of mice with hyperuricemia. This intervention ultimately reduced uric acid levels in mice through the modulation of multiple pathways, including arginine biosynthesis, taurine and hypotaurine metabolism, and glutathione metabolism. This study lays the foundation for exploring how L. rhamnosus JY027 alleviates hyperuricemia, providing theoretical support for developing probiotic preparations to mitigate hyperuricemia and their application.
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Food Science and Human Wellness
·
2024
·
IF 5.6
Hongyuan Zhao,Xiaoyu Chen,Li Zhang,Fanqiang Meng,Libang Zhou,Zhaoxin Lu,Yingjian Lu
Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis, but the adverse effects of hyperuricemia on the liver have been seriously neglected. This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo . Cell free extracts of high dose L. rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1 % to 14.9 % by inhibiting NLRP3 recruitment, which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder. In purine dietary induced hyperuricemia (PDIH) mice model, liver oedema and pyroptosis were ameliorated after L. rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3, caspase-1 and gasdermin-D from 1.61 to 0.86, 3.15 to 1.01 and 5.63 to 2.02, respectively. L. rhamnosus Fmb14 administration restored mitochondrial inner membrane protein (MPV17) and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice, indicating that dysbiosis of mitochondrial membrane potential was restored in liver. Intriguingly, PDIH pyroptosis stimulates the process of apoptosis, which leads to severe leakage of hepatocytes, and both of pyroptosis and apoptosis were decreased after L. rhamnosus Fmb14 treatment. Therefore, L. rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-12
·
IF 3.8
Cheng Shumin,Cui Hongchang,Zhang Jingyan,Wang Qian,Duan Zhi
The main objective of this study was to investigate the potential probiotic properties of Lacticaseibacillus rhamnosus VHProbi®M15 (M15). This study examined the effects of M15 on sucralfate-induced constipation in a mouse model. The BALB/c mice were randomly divided into four groups: the normal group (NOR) was without any treatment, while the constipation (CON), phenolphthalein (PHE), and probiotic (PRO) treatment groups were fed with sucralfate until the appearance of constipation symptoms. Afterward, the NOR and CON groups were given 1 ml saline orally every day until the end of the experiment; the PHE and PRO groups were given phenolphthalein or M15 suspension in 1 ml orally, respectively. Compared with the CON group, the fecal water content and intestinal peristalsis improved in the PRO group. Here, intake of M15 effectively attenuated sucralfate-induced constipation, recuperated colonic epithelial integrity, and increased serum levels of gastrointestinal excitatory neurotransmitters (motilin, gastrin, substance P). Analysis of the intestinal microbiota of mice by 16S rRNA metagenomic revealed an increase in the relative abundance of Bacteroides and a decrease in Sclerotinia, Verrucosa and Proteus in the PRO group. Compared with the CON group, the constipation-induced intestinal microecological changes were partially recovered in the PHE and PRO groups. These results demonstrate that M15 enhanced gastrointestinal transit and alleviated in mice with sucralfate-induced constipation.
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Nutrients
·
2026
·Vol. 18
·期 9
·1335
·
IF 5.8
Tingchao He,Qidong Lu,Jian Zhang,Xinyu Xie,Xin Liu,Hua Jiang,Jing Li,Yumei Zhang
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease linked to epidermal barrier dysfunction, Th2-skewed immune polarization, and disrupted gut microbiota homeostasis. While probiotic interventions show promise in managing AD, the mechanisms governing strain-specific efficacy—particularly systemic modulation via the “gut–skin axis”—remaining to be fully elucidated.Methods: This study systematically compared the oral therapeutic effects of threeLacticaseibacillus rhamnosusstrains (MG-A047, MG-A054, and LGG) in a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model.Results: By integrating behavioral, histopathological, and serological assessments with 16S rRNA-based gut microbiota profiling and in vitro functional assays, this study offers a multidimensional evaluation of the strain-specific advantages and potential therapeutic mechanisms of threeL. rhamnosusstrains. The results demonstrate that MG-A054 most effectively alleviated cutaneous inflammation and pruritus, significantly reduced serum IgE and IL-4 levels, and attenuated epidermal hyperplasia and inflammatory cell infiltration (including mast cells and eosinophils). Mechanistically, this strain may directly inhibit hyaluronidase activity and mast cell degranulation, and specifically remodel the gut microbiota structure, thereby promoting a shift toward a healthier functional profile.Conclusions: These findings suggest that the superior efficacy of MG-A054 may be achieved through coordinated modulation of the gut–skin axis and related pathways. This study offers new mechanistic clues for understanding the strain-specific actions of probiotics and lays a preclinical foundation for the further development of MG-A054 as a potential targeted microecological therapy for AD.
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Food & Function
·
2024
·
IF 5.1
Xiaoqi Pang,Bingqian Zhou,Jingyi Wu,Qi Mo,Lijiao Yang,Tiaotiao Liu,Ge Jin,Lan Zhang,Xiang Liu,Xin Xu,Bangmao Wang,Hailong Cao
Consuming probiotic products is a solution that people are willing to choose to augment health. As one of the global health hazards, sleep deprivation (SD) can cause both physical and mental diseases. The present study investigated the protective effects of Lacticaseibacillus rhamnosus GG (LGG), a widely used probiotic, on SD mice model. Here, it’s shown that SD induced intestinal damage in mice, while LGG supplementation attenuated disruption of the intestinal barrier and enhanced antioxidant capacity. Microbiome analysis revealed that SD caused dysbiosis in the gut microbiota, characterized by increased levels of Clostridium XlVa, Alistipes, and Desulfovibrio, as well as decreased levels of Ruminococcus, which were partially ameliorated by LGG. Moreover, SD resulted in elevated pro-inflammatory cytokine concentrations in both the intestine and brain, while LGG provided protection in both organs. LGG supplementation significantly improved locomotor activity in SD mice. Although heat-killed LGG showed some protective effects in SD mice, its overall efficacy was inferior to live LGG. In terms of mechanism, it was found that AG1478, an inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase, could diminish the protective effects of LGG. In conclusion, LGG demonstrated the ability to alleviate SD-induced intestinal barrier dysfunction through EGFR activation and alleviate neuroinflammation.
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Food Bioscience
·
2024
·
IF 4.8
Xueting Zhao,Peng Cai,Shijin Xiong,Benliang Wei,Tonghao Du,Tao Huang,Qiang Yu,Mingyong Xie,Tao Xiong
Hyperuricemia (HUA) has emerged as a public health burden. Recently, lactic acid bacteria (LAB) were reported to possess potential for alleviating HUA. However, LABs with anti-HUA potential are still scarce, considering which, as well as complex and unclear alleviating mechanisms, and it is worthy to find various LABs with anti-HUA and investigate the alleviating mechanisms. In this study, Lacticaseibacillus rhamnosus NCUH061012 (LR012) with anti-HUA potential was obtained by isolating from infant feces, screening in vitro and evaluating in mice, and integrative fecal microbiome and metabolome were used for the first time to reveal the underlying mechanism of LAB in alleviating HUA. LR012 supplementation was effective in lowering the level of uric acid (UA), which prompted the decrease of serum uric acid (SUA) by 37.20% % compared with the HUA model group. LR012 facilitated the growth of Lactobacillus and Candidatus_Saccharimonas , and decreased the enrichment of Bacteroides , along with increasing short-chain fatty acids and decreasing lipopolysaccharide . Analysis of fecal metabolomics showed LR012 regulated more intestinal metabolites, including adenosine, hypoxanthine, xanthine, succinate, and tryptophan . Furthermore, these alterations in intestinal microbiota and metabolites showed positive associations with improving systemic inflammation and kidney damage, regulating UA production and excretion, and reducing SUA levels. It is deduced that the underlying mechanism of LR012 in alleviating HUA was to modulate intestinal microbiota and metabolites. In conclusion, LR012 was promising in preventing and alleviating HUA as a diet supplement, and these findings underscored the transformative potential of LR012 in alleviating HUA via intestinal remediation.
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JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
·
2024
·
IF 3.2
Wysocki Łukasz,Adamczuk Patrycja,Bardadyn Paula,Gabor Anna,Jelonek Karolina,Kudelska Monika,Kukuć Maksymilian,Piasek Adrianna,Pietras Marta,Słomka Monika,Trojan Zoja,Tybulczuk Wiktoria,Sobiepanek Anna,Żylińska-Urban Joanna,Cieśla Joanna
Spent coffee grounds (SCG) are commercial waste that are still rich in numerous valuable ingredients and can be further processed into useful products such as coffee oil, antioxidant extract, lactic acid, and lignin. The challenge and innovation is to develop the SCG processing technology, maximizing the use of raw material and minimizing the use of other resources within the sequential process. The presented research is focused on the aspect of biotechnological production of lactic acid from SCG by using the Lacticaseibacillus rhamnosus strain isolated from the environment. Thanks to the optimization of the processes of acid hydrolysis, neutralization, enzymatic hydrolysis of SCG, and fermentation, the obtained concentration of lactic acid was increased after 72 hr of culture from the initial 4.60 g/l to 48.6 g/l. In addition, the whole process has been improved, taking into account the dependence on other processes within the complete SCG biorefinery, economy, energy, and waste aspects. Costly enzymatic hydrolysis was completely eliminated, and it was proven that supplementation of SCG hydrolysate with expensive yeast extract can be replaced by cheap waste from the agri-food industry.One-Sentence SummaryA process for efficient lactic acid production from spent coffee grounds using the Lacticaseibacillus rhamnosus strain was developed and optimized, including nutrient solution preparation, supplementation and fermentation.
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Foods
·
2026
·Vol. 15
·期 3
·409
·
IF 5.1
Hongyuan Zhao,Zihan Zhang,Xiaoyu Chen,Chao Tang,Li Song,Zhaoxin Lu,Yingjian Lu
Fructose dietary intake is one of the most common risk factors for hyperuricemia, which is a critical threat to human health, and the lack of an effective biological intervention method is the main problem in preventing hyperuricemia caused by fructose intake.Lacticaseibacillus rhamnosusFmb14 (L. rhamnosusFmb14) has a fructose-metabolizing ability to produce extracellular polysaccharides (EPSs), and the yield of EPSs reached 0.50 and 0.42 g/L after 48 h of fermentation in liquid media of glucose-MRS and fructose-MRS. Six pure polysaccharide components were obtained after purification. A hyperuricemic mouse model was subsequently established by feeding a 60% high-fructose diet with potassium oxyazinate for 8 weeks, and the results revealed thatL. rhamnosusFmb14 and fructose-derived EPS (F-EPS) intervention significantly reduced the serum uric acid level of the model mice from 133.6 μmol/L to 106.7 to 111.0 μmol/L. The content of XOD in the liver decreased from 2188.1 ng/L in the model group to 1797.9 ng/L in the H-Fmb14 group and 1906.6 ng/L in the H-F-EPS group, alleviating fatty liver degeneration and improving intestinal barrier (increasing OCLN and ZO1 expression in colon). The abundances ofallobaculum,bacteroides,Lactobacilli prevotella, andclostridium, the new potential biomarkers of fructose-induced hyperuricemia, were found to be modulated after Fmb14 and F-EPS intervention. The effects of Fmb14 and F-EPS in reducing uric acid synthesis and protecting the intestinal tract are very promising as food intervention agents in the prevention of hyperuricemia caused by fructose dietary.
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npj Biofilms and Microbiomes
·
2025
·Vol. 11
·期 1
·1-17
·
IF 9.2
Yang Ruili,Xu Yi,Xu Jinchun,Huang Chengqing,Zhu Feng,Wang Tian,Kong Rui,Xiao Jie,He Biao,Gu Xiaozhen,Wang Hui-Li
As a prevalent neurodevelopmental disease, whether ASD (autism spectrum disorder) can be ameliorated by the early use of a single microbe remains unknown. Here we used a lactobacillus strain, Lacticaseibacillus rhamnosus GR-1 (LGR-1), for prenatal intervention in autism-like mice with either environmental or idiopathic origins by exclusively administering to the pregnant dams at a dose of 10 9 /mouse/day, followed by offspring behavioral assessment with 3-chamber trial and marble burying test. The results revealed that LGR-1 prevented the occurrence of autism-like symptoms, as evidenced by the improved behaviors and restored E/I (excitatory-inhibitory) balance in the prefrontal cortex of male pups. In parallel, the offspring microbiome was reshaped by LGR-1 treatment, probably mediated by the vertical transmission of maternal microbiome, with its roles further unraveled by fecal microbiota transplant and cross-fostering experiments. In addition to gut commensals, the LGR-1-shaping vaginal microbiota also contributed to the establishment of “beneficial” microbiome. Regarding key taxa in offspring, Akkermansia muciniphila was influenced by LGR-1 and exerted impact on behaviors via pathways related to IL-17-producing lymphocytes. Our findings demonstrate that prenatal microbial administration protects offspring against autism-like behavioral phenotypes through microbiome transmission, highlighting a potential microbe-based therapeutic avenue to mitigate ASD risk.
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Food Bioscience
·
2026
·
IF 6.2
Qinqin Li,Lingxing Feng,Muhammad Yousaf,Dongmei Liu
Obesity is a complex metabolic disorder, and its treatment remains ongoing challenges worldwide. Exopolysaccharides (EPS) produced by lactic acid bacteria are increasingly recognized as potential prebiotics for combating obesity. This study purified EPS2 from L. rhamnosus LR-ZB1107-01 and revealed it as a heteropolysaccharide (Mw 8.42 × 10 4 Da) with 92.5% mannose and 7.43% glucose in molar composition. EPS2 featured a backbone of alternating →2)-α-D- Man p -(1→ and →2,6)-α-D-Man p -(1→ glycosidic linkages, with side chains consisting of α-D-Man p -(1→ units attached at the O-6 positions of the latter residues. In vivo tests demonstrated that EPS2 effectively alleviated obesity-related symptoms, ameliorated dyslipidemia, and inhibited hepatic oxidative damage and lipid deposition. Additionally, EPS2 modulated gut microbiota composition, increasing the abundances of beneficial genera such as Parabacteroides and Candidatus Saccharimonas strongly linked to the alterations in fecal metabolites like arterolane and glycerol tributanoate. The potential of EPS2 in obesity treatment through gut microbiota regulation and metabolic reprogramming provided new insights for the development of anti-obesity strategies.
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Food Bioscience
·
2026
·
IF 6.2
Yalin Li,Ziwei Feng,Liu Liu
Free probiotic cells are vulnerable to environmental and gastrointestinal stresses, which limits their functional efficacy and highlights the need for effective food-grade delivery systems. In this study, cellulose–chitosan microcapsules containing Lacticaseibacillus rhamnosus CICC 6151 ( L. rhamnosus 6151) were prepared using cellulose extracted from soybean dregs, Pueraria residue, Astragalus residue, and pomegranate peel. Among the four formulations, the soybean dregs cellulose-based formulation showed the highest encapsulation efficiency (51.3%), compared with 44.0%, 45.8%, and 42.3% for the other formulations, and was selected for in vivo evaluation. Free L. rhamnosus 6151 reached the stationary phase at approximately 12 h, whereas the encapsulated formulations reached the stationary phase after 19 h. In a loperamide-induced constipation mouse model, the selected microcapsule restored body weight and food intake to 92.22% and 93.94% of the blank control level, respectively, compared with 89.76% and 88.13% in the free-bacteria group and 88.47% and 86.98% in the carrier group. The microcapsule also improved constipation-related phenotypes, alleviated intestinal and liver histopathological injury, modulated colonic inflammatory mediators and serum neuropeptide-related indicators, and was associated with altered gut microbiota profiles. These findings suggest that soybean dregs-derived cellulose is a promising food-grade carrier and that the corresponding cellulose–chitosan microcapsule holds great potential to support the in vivo physiological efficacy of L. rhamnosus 6151 in alleviating constipation-associated symptoms.
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