BRAIN BEHAVIOR AND IMMUNITY
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2026
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IF 7.5
Tingyi Zhou,Yi Yue,Juncai Leng,Yiming Jiang,Han Liu,Shiqi Zhang,Zhengchao Yang,Wei Zhao,Li Li
Lactobacillus plantarum enhances cognition in both normal and antibiotic-treated mice. • Microbial modulation elevates fecal acetate and reshapes brain immune homeostasis • Acetate supplementation alone improves cognition and restores microglial homeostasis. • Acetate enhances microglial homeostasis by reducing mitochondrial oxidative stress. • Acetate activates ACSS2-dependent acetyl-CoA production to reduce microglial inflammation. Gut microbiota profoundly influences cognition through immune and metabolic pathways, but how specific strains contribute to these processes remain unclear. Here, we show that supplementation with Lactobacillus plantarum C067 enhanced spatial and fear-associated memory, while restoring hippocampal cytokine expression and microglial morphology in both healthy and antibiotic-treated mice. L. plantarum also reshaped microbial composition and elevated short-chain fatty acids, particularly acetate. Acetate supplementation replicated the cognitive benefits and restored microglial homeostasis. Mechanistically, acetate exerted anti-inflammatory effects via acetyl-CoA synthetase 2 (ACSS2)–dependent acetyl-CoA production in microglia, reducing mitochondrial oxidative stress and cytokine release. Together, these findings identify an ACSS2-mediated microbe–metabolite–microglia axis that underlies the cognitive benefits of L. plantarum , providing a mechanistic framework for probiotic- and metabolite-based interventions against cognitive decline. Download: Download high-res image (148KB) Download: Download full-size image
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FOOD CHEMISTRY
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2021
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IF 7.514
Shaofeng Yuan,Fangwei Yang,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
Bioremediation of pesticides in contaminated foodstuffs using probiotics has attracted great attention in recent years, but some intermediate products may have profound effects on the toxicity of treated food. Therefore, this work studied the degradation mechanism of dimethoate in milk by L. plantarum, and analyzed the toxicity of degradation products. The results showed that under the optimal conditions, L. plantarum can degrade 81.28% of dimethoate. Dimethoate had high binding affinities to phosphatase with the free energy of –16.67 kcal/mol, and amino acid residues, Gln375 and SER415 played important roles in the catalysis process. Five degradation products were identified using UPLC-QTOF/MS, and their toxicity was estimated using quantitative structure–activity relationship models. Some intermediate products were predicted to be toxic, which should not be ignored, but the overall toxicity of milk decreased after fermentation. Furthermore, the pH and titratable acidity of the fermented milk were 4.25 and 85 ◦ T, respectively.
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Polymers
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2020
·Vol. 12
·期 7
·1565
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IF 3.426
Hongliang Yu,Weihua Liu,Dongmei Li,Chunhong Liu,Zhibiao Feng,Bin Jiang
With the increased interest in information on gut microbes, people are realizing the benefits of probiotics to health, and new technologies to improve the viability of probiotics are still explored. However, most probiotics have poor resistance to adverse environments. In order to improve the viability of lactic acid bacteria, polylactic acid (PLA) nanofibers were prepared by coaxial electrospinning. The electrospinning voltage was 16 kV, and the distance between spinneret and collector was 15 cm. The feed rates of the shell and core solutions were 1.0 and 0.25 mL/h, respectively. The lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers with PLA and fructooligosaccharides (FOS) as the shell materials. Scanning electron microscopy, transmission electron microscopy, and laser scanning confocal microscopy showed that lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers successfully. The water contact angle test indicated that coaxial electrospun nanofiber films had good hydrophobicity. An in vitro simulated digestion test exhibited that the survival rate of lactic acid bacteria encapsulated in coaxial electrospun nanofiber films was more than 72%. This study proved that the viability of probiotics can be improved through encapsulation within coaxial electrospun PLA nanofibers and provided a novel approach for encapsulating bioactive substances.Keywords:coaxial electrospinning;polylactic acid;probiotics;nanofibers
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Journal of Functional Foods
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2023
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IF 5.6
Chongwei Yu,Jingtianyi Liu,Zonghao Liu,Qinchao Ding,Qinchao Zhu,Nan Chen,Jinfeng Fu,Teresa G. Valencak,Daxi Ren
Probiotics have been shown to inhibit the development of IBD but the specific mechanism remains unclear. In this study, the mitigating effect and underlying mechanisms induced by Lactobacillus plantarum ZJUIDS04 ( L. plantarum ZJUIDS04) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice were investigated. After L. plantarum ZJUIDS04 feeding, symptoms of DSS-induced colitis were remarkably reduced, including weight loss, colon shortening, colonic tissue damage, decreased tight junction protein, and pro-inflammatory cytokines. Furthermore, L. plantarum ZJUIDS04 significantly altered the structure of the intestinal microbiota, including decreasing the abundance of Romboutsia , Escherichia − Shigella , and Parabacteroides, as well as increasing the abundance of Akkermansia and Ruminococcus _1. More importantly, the genera of butyric acid-producing bacteria as well as short-chain fatty acids (SCFAs) were increased after L. plantarum ZJUIDS04 intervention, indicating regulation of gut microbiota and its metabolism might be an important factor for L. plantarum ZJUIDS04 alleviation against colitis.
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INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
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2015
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IF 1.206
Chuan Li,Shao-Ping Nie,Ke-Xue Zhu,Tao Xiong,Chang Li,Joshua Gong,Ming-Yong Xie
Lactobacillus plantarum, as a probiotic, has many functional properties in human intestinal tract. This study examined the effects of L. plantarum NCU116 on loperamide-induced constipation in a mouse model. Loperamide (5 mg kg−1) was injected subcutaneously to induce constipation. Animals were divided to five groups: normal group, constipation group, constipation plus three doses of L. plantarum NCU116 groups (NCU116-L, 107 CFU/mL; NCU116-M, 108 CFU/mL; NCU116-H, 109 CFU/mL; respectively). Mice were treated with the probiotic for 15 d to assess the anti-constipation effects. Fecal parameters, intestinal transit ratio and the production of fecal short chain fatty acids, histological of colon and immunohistochemical in colonic interstitial cells of Cajal (ICC) by c-kit were all improved in L. plantarum NCU116-treated mice as compared to the constipation group. These results demonstrate that L. plantarum NCU116 enhanced gastrointestinal transit and alleviated in mice with loperamide-induced constipation.
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FOOD HYDROCOLLOIDS
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2016
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IF 3.858
Wei Li,Ya Zhu,Feng Ye,Bin Li,Xiaogang Luo,Shilin Liu
Cellulose-based microgels, prepared by the sol-gel transition method, were evaluated to extent the loading capacity and make more controlled release of probiotics . Granules of calcium carbonate as porogenic agents made the cellulose microgels with different porosity, and the S BET of modified cellulose microgels was increased to 197 m 2 /g. The microgels were constituted by the interconnected network of the cellulose nanofibrils, which was house-like. The house-liked microgels had the ability for the accommodation of Lactobacillus plantarum ( L. plantarum ) as high as 10 10 cfu/g, and the released viable cells was up to 3.10 × 10 10 cfu/g. Furthermore, the Ca-alginate shell protection of the cellulose houses kept the loaded L. plantarum cells from liberating into stomach but released the cells at intestinal tracts due to the effect of pH-responsibility. From the cross-sectional fluorescence image, L. plantarum cells mainly focused on the cellulose microgels houses instead of Ca-alginate shell. It indicated that the surface modification of the cellulose microgels houses by using alginate based hydrogel exhibited more controlled release of L. plantarum cells, which could be increased to 5.80 × 10 8 cfu/mL at 360 min. Prolonged duration in simulated intestine fluid contributed to making sure that cells reached the desired region of intestine tracts. The designed composite system advances in affording better shelter for probiotic residence or other bioactive nutrients.
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Food Bioscience
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2026
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IF 6.2
Siru Zhao,Jiashuo Chen,Yueying Zhao,Shuang Zhang
Metal-phenolic networks (MPNs) exhibit rapid film formation and surface modification potential, but their material selection and mechanisms are insufficiently explored. This study used gallic acid (GA), epigallocatechin (EGC), and epigallocatechin gallate (EGCG) as polyphenol precursors to construct MPNs coatings on Lactobacillus plantarum ( L. plantarum ) via coordination with ferric (Fe 3+ ) or calcium (Ca 2+ ) ions. Systematic analysis showed that polyphenol structure and metal ion (Fe 3+ > Ca 2+ ) significantly affect coating performance, with EGCG-Fe 3+ coatings achieving the greatest thickness (1584 nm), strongest metal-phenolic coordination, and highest stability. This coating also demonstrated remarkable functional enhancements: superior antioxidant capacity (75.9% DPPH and 98.96% ABTS scavenging rates), high antibacterial rate (78.7%), and excellent gastrointestinal tolerance, maintaining viable cell counts above 10 7 CFU/mL throughout digestion stages. These findings clarify MPNs formation mechanisms, laying a theoretical basis for optimizing polyphenol-metal coordination in probiotic encapsulation systems to enhance functionality and expand applications in probiotic food development.
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Shijing Dong,Liping Guo,Chenyang Xuan,Chenhui Zhu,Zongze Han,Nian Chen,Xinyu Wang,Meng Shen,Xiaoxuan Wei,Hui Yang,Xue Zhang,Man Liu,Simin Zhou,Lu Zhou
Lactobacillus plantarum (L. plantarum ) has shown protective effects in various liver diseases, yet its therapeutic potential and mechanisms in intrahepatic cholestasis remain unelucidated. This study aimed to investigate the therapeutic efficacy and mechanism of L. plantarum in intrahepatic cholestasis using two well-established mouse models induced by α-naphthylisothiocyanate (ANIT) and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). The results indicated that L. plantarum significantly alleviated cholestasis-related hepatic inflammation, ductular proliferation, fibrosis, and improved gut barrier integrity. The 16S rRNA sequencing and untargeted metabolomics analysis revealed that the intestinal abundance of L. plantarum was significantly increased after intervention and showed a positive correlation with the level of indole-3-acetic acid (IAA), a key functional metabolite of tryptophan ( r = 0.712, p = 0.003). The elevated IAA levels were closely associated with the improvement of key liver injury indicators. Exogenous IAA supplementation replicated the hepatoprotective effects of L. plantarum in intrahepatic cholestasis. Transcriptome analysis indicated that IAA mediated these benefits primarily through aryl hydrocarbon receptor (AHR) activation, and AHR inhibition markedly attenuated its protective effects. This study reveals that L. plantarum ameliorates intrahepatic cholestasis by enhancing tryptophan metabolism to promote IAA production, which in turn activates the IAA-AHR axis, providing new insights into probiotic and metabolite-based therapies for intrahepatic cholestasis.
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FOOD CHEMISTRY
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2021
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IF 7.514
Shaofeng Yuan,Changjian Li,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
Organophosphorus pesticides (OPPs) are widely used worldwide, leading to varying degrees of residues in food. Lactic acid bacteria (LAB) can degrade OPPs by producing phosphatase. This study explored the reasons for the variation in the degradation of different OPPs by Lactobacillus plantarum . The results showed that the degradation effects of OPPs by L. plantarum (intact cells) varied greatly, the degradation rate constant of phoxim was 1.65-fold higher than that of dichlorvos. However, the phosphatase extracted from L. plantarum had no degradation selectivity for OPPs in vitro . It was speculated that the selective uptake of cells determines this degradation selectivity. The results of molecular docking supported this hypothesis because there was no difference in the binding energies between phosphatase and OPPs, while the binding energies between phosphate-binding protein and pesticides were different, and they were negatively correlated with the degradation rate constants of the eight OPPs by L. plantarum .
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JOURNAL OF DAIRY SCIENCE
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2020
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IF 3.333
Yuan Gao,Yujun Liu,Mengying Sun,Heping Zhang,Guangqing Mu,Yanfeng Tuo
ABSTRACT In our previous studies, Lactobacillus plantarum Y44 showed antioxidant activity and favorable gastric and intestinal transit tolerance. In the current study, we investigated the physiological function of L. plantarum Y44 based on an analysis of its genotype and phenotype. The complete genome of L. plantarum Y44 contained a single circular chromosome of 3,255,555 bp, with a GC content of 44.6%, and a single circular plasmid of 51,167 bp, with a GC content of 38.8%. The L. plantarum Y44 genome contained 3,293 genes including 3,112 protein coding sequences, 16 rRNAs, 66 tRNAs, 4 small (s)RNAs, and 95 pseudo genes. Lactobacillus plantarum Y44 could metabolize 24 different carbohydrate sources. Nineteen complete phosphoenolpyruvate-dependent sugar phosphotransferase system complex genes and intact Embden-Meyerhof-Parnas pathway and hexose monophosphate pathway enzyme genes, as well as abundant carbohydrate active enzyme genes, were identified in the L. plantarum Y44 genome. We also identified genes related to the biosynthesis of exopolysaccharide and surface proteins. Surface proteins played an important role in the L. plantarum Y44 adhesion to HT-29 cell monolayers, as evidenced by the removal of cell surface proteins leading to decreased adhesion capacity. The L. plantarum Y44 genome contained genes encoding chaperones, intracellular proteases, and 2-component systems, which were associated with the general stress response. Genes encoding bile salt hydrolase, F 0 F 1 -ATPase, Na + /H + -antiporter, H + /Cl − exchange transporter, cyclopropane-fatty acyl-phospholipid synthase, and alkaline shock protein were identified in the L. plantarum Y44 genome, which might explain the strain's favorable gastric and intestinal transit tolerance. Some genes associated with encoding the NADH system, glutathione system, and thioredoxin system were predicted via in silico analysis and might account for the strain's ability to scavenge reactive oxygen species. Lactobacillus plantarum Y44 was susceptive to 7 antibiotics and did not produce biogenic amines, likely due to the absence of acquired antibiotic resistance genes and amino acid decarboxylase genes. The phenotype profile of L. plantarum Y44 was associated with its genetic characteristics, indicating that strains with certain physiological functions can be screened by analyzing their phenotypic and genotypic characteristics. Lactobacillus plantarum Y44 has the potential to be used as a starter culture in fermented dairy products.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2019
·
IF 3.714
Xiaoyu Yang,Yue Su,Liang Li
The change of protein structure and intermolecular interaction during the formation of soy protein isolate (SPI) gel induced by Lactobacillus plantarum ( L . plantarum ) were investigated. In the present study, the peak value of UV–Vis spectra decreased gradually with the prolongation of fermentation time. Fermentation treatment resulted in α-helix content decreasing by 60.16%, while β-sheet content increased by 68.15% (P < 0.05). The surface hydrophobicity was decreased by 62.31% (P < 0.05) due to the molecular aggregation of SPI. Correspondingly, the free sulfydryl group content decreased by 58.48% (P < 0.05). These results highlighted that the protein structure and intermolecular interaction of SPI could be significantly changed by L . plantarum fermentation. The main intermolecular interactions maintaining the fermentation-induced gel were hydrophobic interactions and disulfide bonds. This work provided theoretical support for the establishment of the mechanism of fermentation-induced gel and the rational application of L . plantarum in soybean products.
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BIOMEDICINE & PHARMACOTHERAPY
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2023
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IF 7.5
Zhibo Zeng,Zonghao Huang,Wen Yue,Shah Nawaz,Xinzhu Chen,Jing Liu
Gut microbiota (GM) contributes to the production of immune-regulatory molecules and cytokines. However, our understanding regarding intricate relationship between Lactobacillus plantarum and GM on regulation of immune function remained limited. To investigate the effect of Lactobacillus plantarum on an immunosuppressed mouse model, we employed cyclophosphamide treatment and conducted various analysis including H&E (hematoxylin-eosin staining), immunohistochemistry, 16S rRNA gene sequencing, and RT-PCR. Our results demonstrated that the administration of Lactobacillus plantarum had significant immunoenhancing effects in the immune-suppressed mice, as evidenced by the restoration of functional expression of specific immune markers in the spleen and an increase in the number of goblet cells in intestine (P < 0.05). Microbial taxonomic analysis revealed alterations in the gut microbiota composition, characterized by a decrease in the richness of Firmicutes and an increase in the proportion of Verrucomicrobia and Actinobacteria following cyclophosphamide treatment. Furthermore, cyclophosphamide treatment significantly suppressed the mRNA expression of inflammatory cytokines (P < 0.05), which were subsequently restored after administration of Lactobacillus plantarum . These observations provide valuable insights into the complex interplay between probiotics, gut microbiota, and immune system functioning.
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