LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
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IF 4.006
Yanbo Wang,Congnan Cen,Jian Chen,Chaosheng Zhou,Linglin Fu
Essential oils have attracted increasing attentions due to their broad-spectrum antimicrobial activities and generally recognized as safe (GRAS) regulatory status. However, their low-solubility in water and reduced effectiveness in food matrices limit the commercial application in food industry. In this study, we used ultrasonic emulsification , medium-chain triacylglycerol (MCT) and phosphate-buffered saline (PBS) to formulate a highly stable and water-dispersible litsea cubeba essential oil (LCEO) nanoemulsions. After emulsification, a minimizing droplet size of LCEO nanoemulsions was achieved at approximately 100 nm in diameter, resulting in an increase in the water solubility and better stability at 4 °C and 25 °C. Furthermore, the bioactivities (antibacterial, antioxidant and antibiofilm properties) of LCEO nanoemulsions were greatly improved after emulsification, suggesting that nano-emulsification is beneficial for the value development of various essential oils and the LCEO nanoemulsions could be used as a novel food preservative for controlling bacterial growth and preventing oxidative deterioration in foods.
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高影响因子
JOURNAL OF ESSENTIAL OIL RESEARCH
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2013
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IF 0.553
Xiao-Wei Huang,Yun-Chao Feng,Yi Huang,Hai-Ling Li
Litsea cubeba essential oil (LCEO) is widely used as a flavor enhancer in cosmetics and as a folk skin-care agent in southern China. The objective of this paper is to investigate whether LCEO has biological activities that might be useful in modern skin-protection formulations. The present study results revealed that citral (57.4%) was a major component, while LCEO exhibited a potent inhibitory effect on tyrosinase (IC50 values 100 μg/mL), good antioxidative activities (IC50: 17.75 mg/mL for ABTS+., 10.2 mg/mL for O2
−), and apparent protective effect against UV–TiO2–NO2
−-induced protein oxidation at 0.01 mg/mL and tyrosine nitration at 0.1 mg/mL. Additionally, the biological activities of LCEO were compared with that of its main constituents. The results demonstrated that any individual major component was not the unique contributor to the high activity of LCEO. Based on these results, we suggested that LCEO could serve as a new natural skin-whitening agent.
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高影响因子
JOURNAL OF FOOD QUALITY
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2020
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IF 1.763
Shiqi Xia,Hong Lin,Peilin Zhu,Peiling Wang,Shengliang Liao,Shangxing Chen,Zongde Wang,Guorong Fan
Aspergillus flavus (A. flavus) is a frequent harmful fungal pathogen. It can infect traditional Chinese medicine materials and release aflatoxin, to cause both economic and human health effects. By comparing the inhibitory potential of Litsea cubeba oil and its active components to A. flavus CGMCC 3.4408, citral was confirmed to be the main component that inhibits the growth of A. flavus CGMCC 3.4408, and the EC50 was 163.65 mg L−1. The inhibitory effect of citral on A. flavus CGMCC 3.4408 was studied for colony growth rate, mycelium biomass, aflatoxin production, and microstructure. Citral slowed down the growth rate of colonies and reduced mycelium biomass and toxin production. Moreover, citral altered the morphology of fungal spores and mycelium. In addition, citral also has the inhibitory effects on the isolates of A. flavus from moldy traditional Chinese medicinal materials. Thus, citral can be used as a potential agent to check the growth of A. flavus or related fungal strains.
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高影响因子
BIOMASS & BIOENERGY
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2025
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IF 5.8
Kaili Zhang,Xiao Jiang,Ran Tao,Jingcong Xie,Jian Zhao,Ning Zhang,Yicun Chen,Yangdong Wang,Kui Wang,Jianchun Jiang
Lignin is an important component of plants, and its utilization is essential for biorefinery. Litsea cubeba fruit residues (LSRs) are abundant renewable lignin-rich bioresource with enormous potential for producing value-added monophenols. Herein, the lignin from LFRs was isolated with organic solvent, which was characterized by two-dimensional HSQC-NMR, FT-IR, Py-GCMS and GPC. The results indicate that the lignin in LFRs is mainly composed of guaiacyl (G) subunits (93 %), with small amounts of syringyl (S)/ p -hydroxyphenyl (H) structural subunits. The reductive catalytic fractionation (RCF) of LFRs was investigated using methanol (MeOH) as the solvent and a commercial Pd/C (5 wt% Pd) catalyst. Two major phenolic compounds, 4- n -propylguaiacol and 4- n -propanolguaiacol derived from β-O-4 structure, were generated with high yield (19.4 wt%). This result was different from G-type pine wood powder, where 4- n -propanolguaiacol was the main product with a selectivity of 93.8 %. Besides, the influences about several important reaction parameters like time, temperature, solvent, H 2 pressure, and catalyst stability were also investigated based on monophenol yields and average molecular weight. Overall, the lignin components of Litsea cubeba fruit residues were identified and transformed into high functional phenolic chemicals in this work.
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高影响因子
RENEWABLE ENERGY
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2021
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IF 8.001
Dongming Li,Wenping Feng,Chao Chen,Shangxing Chen,Guorong Fan,Shengliang Liao,Guoqiang Wu,Zongde Wang
An effective approach for synthesizing green and sustainable biodiesel fuel from low-value vegetable oils has been explored. Herein, a series of catalysts of zinc supported on zirconia ( x Zn/ZrO 2 ) were successfully synthesized, characterized, and applied for the production of biodiesel from Litsea cubeba kernel oil (LCKO) through the transesterification process. The results showed that the monoclinic phase structure of the prepared catalysts transformed to the more stable tetragonal phase structure after supporting different Zn loadings (≥5%). Compared with the pure ZrO 2 , the highly dispersed Zn species on the surface of x Zn/ZrO 2 could be divided into Zn oxide and ZnOH + , which could promote the transesterification of LCKO. Thus, the 7%Zn/ZrO 2 catalyst exhibited the best catalytic activity of the LCKO conversion 82.5%, which was attributed to the positive effects of both the ZrO 2 support and Zn species. Furthermore, the various reaction parameters (such as reaction time, stirring rate, n CH3OH :n LCKO , catalyst amount, and reaction temperature) were investigated systematically and optimized carefully. And a possible mechanism of the transesterification process catalyzed by Zn/ZrO 2 catalysts was also proposed. Finally, the physical properties of the as-synthesized biodiesel accorded with the current international biodiesel standards.
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高影响因子
Journal of Food Measurement and Characterization
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2025
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IF 3.3
Zeng Liping,Yang Ruopeng,Deng Qiuhong,Hu Chunyuan,Tian Ruijin,Yang Guangming,Liu Sha,Fan Aiping
This study successfully prepared polylactic acid (PLA) composite films containing Litsea cubeba oil (LCO, 9 wt%) and different concentrations of nano-TiO 2 (0 wt%, 1 wt%, 2 wt%, and 3 wt%), and evaluated their efficacy in preserving fresh-cut jackfruit. The results showed that LCO and nano-TiO 2 could be successfully incorporated into the PLA matrix, which improved the composite films’ structure, flexibility, water and UV resistance, and antioxidant and antimicrobial activities. Notably, the composite film with 2 wt% nano-TiO 2 content exhibited the best overall performance among all the packaging materials. Experiments on fresh-cut jackfruit preservation revealed that the composite films suppressed the proliferation of microorganisms like total bacterial count, mold and yeast, and to varying degrees, inhibited their growth and slowed down the quality deterioration of fresh-cut jackfruit. In addition, composite film with 2 wt% nano-TiO 2 content was the most effective in inhibiting microorganisms, respiratory rate, enzyme activity, and malondialdehyde (MDA) content as well as in maintaining weight loss, color, firmness, flavor, and vitamin C (VC) content, ultimately extended the shelf-life of fresh-cut jackfruit from 6 to 15 days at 10 °C. This work demonstrates the feasibility of bioactive PLA films as safe antimicrobial packaging for tropical fruits.
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高影响因子
Food Bioscience
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2023
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IF 5.2
Mei Bai,Jinming Dai,Wei Hu,Changzhu Li,Haiying Cui,Lin Lin
This study investigated the use of Litsea cubeba essential oil (LC-EO) as an anti-virulence agent targeting listeriolysin O (LLO) of Listeria monocytogenes ( L. monocytogenes ). The hemolysis assay showed a significant decrease in the hemolytic activity of LLO by 90.7% and 70.2% after exposure to 500 μg mL −1 LC-EO or 700 μg mL −1 citral (the main component of LC-EO), respectively. This suggested that citral plays a major role in the anti-virulence function against LLO. The experiments including UV–visible absorption spectroscopy , fluorescence quenching and three-dimensional fluorescence spectroscopy demonstrated that exposure to citral leads to significant changes in the conformation of LLO, potentially resulting in the unfolding of its structure and the formation of a ground-state complex. Synchronous fluorescence spectra analysis indicated that the binding site of citral could be the undecapeptide region of LLO. Additionally, the results of molecular docking and the acid-base effect experiment of different aldehydes and phenols revealed that the cysteine residue of LLO and the α, β-unsaturated aldehyde of citral are likely to form a covalent bond through Michael addition . This interaction is believed to be the primary reason for the loss of hemolytic activity. As a result, this work provides a new approach for the development of LLO-targeting anti-virulence agents and holds promise for expanding the application of LC-EO in the field of food microbiological safety.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Jiantao Zhang,Ran Tao,Yangdong Wang,Jianchun Jiang,Yicun Chen
The essential oil of Litsea cubeba demonstrates notable antibacterial properties and holds considerable promise for various applications. To explore green and effective extraction method for L. cubeba essential oil, we introduced enzyme-assisted natural deep eutectic solvents (DESs). The optimization of DESs was performed using response surface methodology with a Box-Behnken design (BBD), and the optimal parameters were determined as follows: a choline chloride to citric acid (ChCl/CA) ratio of 1:0.97, water content of 73%, and cellulase(0.5%) enzymolysis temperature of 45°C. Compared to traditional steam distillation, the DESs method significantly increased the essential oil yield from 3.20% to 4.70% and expanded the chemical profile of the essential oil, identifying a total of 79 compounds. Five new aromatic compounds, including methyl geranate, geranic acid, p-mentha-1,5-dien-8-ol, α-terpinyl acetate, and terpinyl acetate, were identified. Antifungal activity against Aspergillus flavus was confirmed by scanning electron microscopy. At a concentration of 1,000 μL/L, after 96 hours of incubation, essential oils extracted using enzyme-assisted DESs method demonstrated substantial inhibition against A. flavus , with the ChCl/EG formulation achieving a 100% inhibition rate. The content of citral, citronellal, citronellol, linalool, and new compound methyl geranate showed a positive correlation with the anti- A. flavus inhibition rate. The enzyme-assisted DESs extraction method proves to be an effective and efficient approach for extracting L. cubeba essential oil and provides a valuable reference for future research and application in essential oil extraction.
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高影响因子
npj Science of Food
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2026
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IF 9.7
Li Li,Wang Wei-qi,Wang Bin,Han Qiu-yue
The protein of oilseed crops can be hydrolyzed into peptides by enzymatic hydrolysis. In our investigation, 9 antioxidant peptides from the protein hydrolysate fraction of Litsea cubeba by RP-HPLC and identified as AN, VEQ, RV, VQ, VHDVSF, FQI, EAALA, VVGGG, and LA, respectively. The in vitro antioxidant assay results showed VHDVSF had the most potent antioxidant activity. Additionally, VHDVSF modulated the kelch-like ECH-associated protein l (Keap1)/ nuclear factor erythroid 2-like 2 (Nrf2) pathway to protect against hepatocellular oxidative stress damage. The ultraviolet-visible absorption and fluorescence spectra analysis indicated that VHDVSF might bind to the recombinant Keap1 protein through hydrogen bond formation and hydrophobic interactions. The results of molecular dynamics simulations (MDS) indicate that the binding to Keap1 protein is spontaneous and driven mainly by electrostatic interaction forces and van der Waals forces, which provided theoretical essential support for exploring VHDVSF as an antioxidant.
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高影响因子
FOOD HYDROCOLLOIDS
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2026
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IF 13.8
Wenliang Qi,Qingqing Jiang,Erihemu
Food packaging plays a crucial role in extending food shelf life and ensuring food quality and safety. However, conventional packaging materials often contribute to environmental pollution, making the development of eco-friendly, edible functional coatings an effective strategy for addressing both food preservation and sustainability concerns. In this study, a novel edible composite coating was developed using tamarind seed polysaccharide (TSP) and flaxseed gum (FG) as the film-forming matrix, with Litsea cubeba essential oil (LCEO) incorporated as an active component. Single-factor optimization identified the optimal formulation as 0.5% TSP, 0.5% FG, and 0.6% LCEO. Structural characterization revealed that TSP and FG formed a stable three-dimensional network, while LCEO was uniformly dispersed within the matrix, significantly enhancing the mechanical and barrier properties of the composite film. The optimized film exhibited excellent antioxidant activity, with DPPH and ABTS radical-scavenging rates of 79.70% and 89.77%, respectively. Furthermore, it demonstrated remarkable antibacterial and antifungal activities against Escherichia coli , Staphylococcus aureus , and Botrytis cinerea . Application tests on postharvest grapes showed that the composite coating effectively reduced weight loss, delayed fruit softening, and retarded nutrient degradation, thereby extending the shelf life of grapes under ambient storage conditions. In addition, the coating was nontoxic, edible, and fully biodegradable, fulfilling the requirements for sustainable food packaging and exhibiting considerable potential for industrial applications. These findings demonstrate that the TSP/FG/LCEO composite coating is a promising sustainable packaging material with multifunctional antioxidant and antimicrobial properties for maintaining postharvest quality and extending the shelf life of fresh produce.
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高影响因子
BMC PLANT BIOLOGY
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2026
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IF 5.6
Liao Guoxiang,Pan Xiaoyi,Ning Xiaodan,Fan Guorong,Chen Shangxing,Zhang Lu,Wang Zongde,Yang Yuling,Wang Jiawei
Background Litsea cubeba is an important industrial plant valued for its pericarp essential oil and kernel oil. However, variation in seed traits and kernel oil characteristics among wild populations and their relationships with ecological factors remain insufficiently understood. Wild germplam from 21 provenances across eight provinces in southern China was evaluated for seed traits, kernel oil content, and fatty acid composition, and variance analysis, correlation and path analysis, and multivariate ecological analyses were used to characterize phenotypic diversity and examine its associations with environmental variables. Results Analysis of variance showed that the investigated provenances differed significantly in seed traits and kernel oil characteristics ( P < 0.05). Provenances ZJ-LY and SC-KJ exhibited relatively high kernel oil content (≥ 35%), whereas YN-XC, GZ-ZY, and SC-KJ showed higher seed yields (SY, ≥ 1000 g.tree −1 ). In addition, kernel oil from Yunnan provenances was characterized by relatively high lauric acid content (C 12:0 , ≥ 60%). Variance partitioning analysis revealed that provenance effects accounted for the majority of variation in seed and kernel oil characteristics. Seed and its kernel oil yield showed greater diversity than its oil content and components. Based on the RDA analyses, annual average relative humidity, annual average rainfall, soil pH, soil maximum water-holding capacity, soil total potassium, soil water content, and longitude were identified as the main environmental correlates of seed and kernel oil characteristics. Greater kernel oil accumulation was associated with higher annual minimum temperature, higher soil total potassium, and higher latitude, whereas less favorable fatty acid composition was associated with higher soil water content and longitude; in addition, greater C 12:0 accumulation was associated with higher annual maximum temperature and higher soil water content. Conclusions These findings reveal substantial variation in seed traits and kernel oil characteristics among wild L. cubeba populations in southern China. Provenances ZJ-LY and SC-KJ are promising candidates for breeding programs targeting high kernel oil content. Moreover, the identified associations of kernel oil accumulation and fatty acid composition with annual minimum temperature, soil water status, and soil nutrients provide useful guidance for germplasm selection and cultivation management.
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PubMed
高影响因子
Journal of Food Measurement and Characterization
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2026
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IF 3.6
Du Ruoyu,Ye Mei,Jin Chong,Li Na,Qi Dianwen,Zhang Haotian,Liu Xuecheng,Dai Jinhang,Yin Zhongyi
This study established a general six-dimensional molecular analysis toolkit (6D MAT) (version 2.0) to comprehensively collect the molecular information of feedstock, extract liquor, and purified extracts derived from Litsea cubeba fruit (LCF), and to connect this data with the activity evaluation results to achieve a structure–activity relationship closed loop. Specifically, we established a streamlined and cost-effective ultra-high performance liquid chromatography (UHPLC) or high performance liquid chromatography (HPLC)-based molecular analysis platform that integrates UV and RID detectors, avoiding dependence on mass spectrometry while ensuring simultaneous qualitative and quantitative identification 17 phenolic and 12 non-phenolic compounds (including 3 monosaccharide, 1 sugar acid, 5 alcohols, and 3 organic acids) and achieving high sensitivity (LODs: 0.02–0.89 μg/mL) and linearity (R2 > 0.997). Principal findings reveal that dried LCF contains exceptionally high extractables (56.55 wt%), with ethanol extraction yielding near-complete efficiency (99.94%) compared to water (64.28%). Notably, water extracts exhibited significantly higher total phenolic content (55.37% vs. 35.33% in ethanolic extracts) and superior bioactivities, including potent antioxidant capacity (DPPH· IC 50 : 0.619 mg/mL; ABTS •+ IC 50 : 0.420 mg/mL) and α-amylase inhibition (6.59% at 9.39 mg/mL). Molecular analysis traced phenolic antioxidants to lignin-derived macromolecules, validating our “molecular traceability” approach. Graphical
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高影响因子
危险品化学品经营许可证（带存储）