COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Menghang Zu,Heliang Song,Jiabin Zhang,Qiubing Chen,Shiqin Deng,Brandon S.B. Canup,Yi Yuan,Bo Xiao
Edible plant-derived nanoparticles (NPs) have attracted increasing attention in the treatment of ulcerative colitis (UC). Lycium barbarum (LB), a popular functional fruit, possesses various biological functions. Here, fat-soluble contents were extracted from LB and further processed into LB lipid-derived NPs (LBLNs). The resultant NPs had an average hydrodynamic diameter around 189.2 nm, narrow size distribution (polydispersity index = 0.2), and negative surface charge (-34.9 mV). Moreover, they could be efficiently taken up by UC therapy-related target cells (macrophages), and over 69.0 % of macrophages internalized LBLNs after 4 h co-incubation. We further found from the in vitro results that LBLNs had strong capacities to inhibit the secretion of the main pro-inflammatory cytokines (TNF-α and IL-12) and up-regulate the expression of the typical anti-inflammatory factor (IL-10). Finally, mice experiments confirmed that LBLNs after oral administration could specifically accumulate into inflamed colon tissues, and further attenuate UC-relevant symptoms ( e.g. , bodyweight loss, colon shortening, increase of spleen weight, and histopathological appearance, as well as ulceration). Collectively, this study demonstrates the excellent therapeutic outcomes of LBLNs against UC and provides a promising edible nanotherapeutic for UC treatment.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Cui Cao,Lilong Wang,Xueqian Zhang,Chunqing Ai,Zhongfu Wang,Linjuan Huang,Shuang Song,Beiwei Zhu
The present study investigated the utilization of an arabinogalactan from Lycium barbarum (LBP-3) by intestinal Bacteroidetes species. The mixed-culture assay showed 58.4 % LBP-3 was utilized, and Bacteroides caccae and Phocaeicola vulgatus utilized more LBP-3 in single-culture compared to others. During in vitro fermentation of LBP-3, P. vulgatus favored arabinose while B. caccae preferred galactose. Moreover, 9 and 25 oligosaccharides were identified by HPLC-MS n in conditioned media (CM) derived from B. caccae and P. vulgatus , respectively. All of 3 tested Parabacteroides species ( P. distasonis , P. goldsteinii , and P. johnsonii ) markedly proliferated in CM of B. caccae and P. vulgatus , and proliferations of B. uniformis , B. finegoldii , B. ovatus and B. thetaiotaomicron also increased significantly in CM of B. caccae. The study suggests that the ability of Bacteroidetes species to degrade LBP-3 and sheds light on cooperative interactions of Bacteroides, Phocaeicola , and Parabacteroides species in the presence of LBP-3.
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International Journal of Food Science
·
2025
·Vol. 2025
·期 1
·4899439
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IF 3.1
Yuling Chen,Xueluan Liu,Genglin Lu,Xuan He,Dandan Li,Yunong Tian,Xiaolin Feng,Yong Ye
This study has established structure-function relationships of Lycium barbarum polysaccharides (LBPs) relevant to antioxidant and tyrosinase-inhibitory functions in food systems by integrating optimized extraction, stepwise purification, comprehensive structural analysis, and functional verification, offering a novel multimethod approach to explore structure-based mechanisms of bioactivity. Firstly, hot-water extraction optimized at 30 mL/g, 90°C, and 5 h yielded 7.44 % ± 0.17 % crude LBPs. Secondly, ion-exchange followed by gel-filtration chromatography separated two purified fractions: LBP-1 (115.1 kDa) and LBP-2 (73.5 kDa). LBP-1 exhibited a compact morphology and featured spectral characteristics indicative of a triple-helix structure, along with both α - and β -glycosidic linkages. In contrast, LBP-2 showed a more amorphous configuration without triple-helix features and predominantly β -linkages. Their monosaccharide profiles also differed, with LBP-1 enriched in glucose, mannuronic acid, galacturonic acid, and glucuronic acid, while LBP-2 contained glucose, galacturonic acid, mannuronic acid, and arabinose. Both fractions exhibited in vitro antioxidant and tyrosinase-inhibitory activities, with LBP-2 demonstrating relatively stronger effects. The enhanced performance of LBP-2 may be associated with its dispersed structure and uronic acid–rich β -linked motifs, which increase the accessibility of functional sites. These insights support the potential application of both LBP-1 and LBP-2 in functional foods, with LBP-2 showing greater promise for use in beverages and fresh-cut fruit products requiring natural antibrowning and antioxidant solutions.
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Forests
·
2021
·Vol. 12
·期 6
·699
·
IF 2.634
Jian Zhao,Te Ma,Tetsuya Inagaki,Qingyu Chen,Zening Gao,Lijuan Sun,Haoxuan Cai,Chao Chen,Chuanlin Li,Shixia Zhang,Satoru Tsuchikawa,Jun Chen
When harvestingLycium barbarumL., excess amounts of detachments of the half-ripe fruit, unripe fruit, flowers, and leaves significantly affect the yield and adversely affect the subsequent processing, such as drying and grading. Finite element method (FEM) simulations and experiments of detachments were performed to harvest more ripe fruit and less half-ripe fruit, unripe fruit, flowers, and leaves. Three-dimensional (3D) models of the ripe fruit, half-ripe fruit, unripe fruit, flowers, leaves, fruit calyxes (flower calyx), fruit stems (flower stem), and branches were constructed using a 3D scanner, and material mechanics models of the above parts were established based on physical tests with universal testing machines. Detachment simulations and experiments of the ripe fruit, half-ripe fruit, unripe fruit, flowers, and leaves were performed to determine the detachment mechanisms and sequences. The detachment forces of each set of two parts were obtained. The field experiments showed that the detachment force between the fruit and calyx of ripe fruit was the lowest value of these forces, and only the ripe fruit was the first to detach from the calyx when harvesting. The results provided data support on the mechanics properties of wood and the optimization basis for the harvesting method ofL. barbarum.
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JOURNAL OF INORGANIC BIOCHEMISTRY
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2020
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IF 3.212
Ying Wang,Qianqian Han,Feng Bai,Qiang Luo,Mingliang Wu,Gang Song,Hongmei Zhang,Yanqing Wang
In this work, the new polysaccharide-platinum conjugates of 5-aminosalicylic acid modified lycium barbarum polysaccharide linking platinum compounds were designed in order to construct an anticancer metal drug delivery system. The multiple analysis methods were used to describe the chemical structure and physical properties of the polysaccharide-metal conjugates . The results showed that 5-aminosalicylic acid successfully acted as linker which was covalently bound between polysaccharide and platinum compound. The morphology and rheological properties of polysaccharide have been changed by the formation of conjugates, which exhibited certain inhibition specificity to A549 (human lung cancer cell line). The agarose gel electrophoresis and fluorescence microscopy results demonstrated that such conjugates promoted the unwinding of DNA and could significantly damage the nucleus of A549 cells. Cell cycle analyzing the Pt complex of conjugates could cause intracellular DNA damage and induced G2 phase arrest . So, polysaccharide-platinum conjugates might find a range of applications, for example in metal anticancer drug delivery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Ze-yong Tang,Dong Sun,Chun-wei Qian,Qi Chen,Shun-shan Duan,Sheng-yun Sun
Nonylphenol is an endocrine disrupting chemicals that can disrupt the organisms’ reproductive system, and exists widely in rivers and lakes. Lycium barbarum polysaccharide (LBP) is the main active constituent (about 10%) in Lycium barbarum , which is used to protect reproductive health. In this study, we investigated whether LBP can alleviate nonylphenol exposure induced testicular injury in juvenile zebrafish. We detected histological alteration, anti-oxidant enzyme profile and P450 gene transcription to assess LBP effect on testicular development. The GSI reduced significantly due to nonylphenol exposure, while LBP can improve the GSI. The densities of sperms increased and non-celluar zone decreased after LBP treatment. Meanwhile, Cyp11b gene was up regulated to NP group, and cyp19a gene was down regulated to NP group. In sum, the LBP could repair the testicular injury in zebrafish. This findings provide a basis research to remit the estrogen effect of artificial endocrine disruptor.
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Food Chemistry-X
·
2024
·
IF 6.5
Fei Hu,Yu-Zhu Song,Jin-Yu Li,Kiran Thakur,Jian-Guo Zhang,Zhao-Jun Wei
This study investigated the effects of Lycium barbarum pulp (LBP) on the properties of mixed dough and gluten protein. The results showed that appropriate addition of LBP (5 %) significantly improved the performance of the dough, promoted the aggregation of gluten protein, enhanced the water binding ability, and delayed the gelatinization of starch during cooking. Compared with the control group, the peak temperature (T p ) of the LBP sample gradually increased from 63.23 °C to 65.56 °C, the expansion force reduced by about 21.56 %, the absolute Zeta potential lowered by about 18.4 %, and the α -helix content and β -folding increased by 32.36 % and 10.23 %, respectively, indicating the more orderly and stable overall structure. However, LBP did not change the crystal configuration of starch and still showed typical type A line diffraction. Moreover, the addition of LBP increased the polyphenol content, which further improved the antioxidant properties and provided the possibility to improve the health potential of the flour.
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CHINESE JOURNAL OF STRUCTURAL CHEMISTRY
·
2025
·
IF 10.3
Lin-An Cao,Yi-Qian Li,Lu Sun,Sai Chu,Yun-Fei Huo,Xiao-Qin Li,Jia-Hui Guo,Fang-Li Yuan,Kenichi Otake,Dai-Lian Wang,Ming-Shui Yao
The sulfur-fumigation process not only induces the chemical transformation of Lycium barbarum (Lb, a widely used traditional Chinese medicine) but also severely influences human health. Given the existing challenges like the complex and time-consuming operation, as well as the high technical demands of the current detection methods for sulfur-fumed Lycium barbarum (SF-Lb), this paper employs a simple chemiresistor to carry out discrimination research between Lb and SF-Lb which have significant differences in volatolomics. The sensor is constructed by a conductive metal-organic framework (cMOF) thin film, Cu 3 (HHTP) 2 , due to its abundant active sites, excellent electron transfer performance as well as the capacity to detect specific groups of volatile organic compounds (volatolomics). Consequently, the response values of the Cu 3 (HHTP) 2 -based sensor to 0.5 g SF-Lb (151.74%) are significantly higher than those to normal Lb (80.07%), identifying SF-Lb simply and rapidly with an accuracy of ∼100%. Our work investigates volatolomics of SF-Lb and establishes a new rapid discrimination method for sulfur-fumed traditional Chinese herbs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Wei Liu,Jinnan Xu,Rui Zhu,Yiqing Zhu,Yang Zhao,Pei Chen,Chun Pan,Wenbing Yao,Xiangdong Gao
Techniques including ultraviolet–visible spectra (UV), high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as N H variable angle vibration of CONH could be selected as herbal markers to distinguish different samples.
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International Journal of Food Engineering
·
2020
·Vol. 16
·期 11
·
IF 1.022
Jitian Song,Shi Dongqi,Su Hang,Feng Yongxia,Tian Wei
Ultrasonic extraction was a new development method to achieve high-efficiency extraction of Lycium barbarum polysaccharides instead of hot water extraction. In this paper, the single factor method combined with the box Behnken design of response surface method was used to study the influence of extraction time, extraction temperature, material liquid ratio and ultrasonic power on the extraction rate of L . barbarum polysaccharide. The results indicated that the best extraction rate of L . barbarum polysaccharide was 12.54 ± 0.12% under the conditions of 80 min for extraction time, 73 °C for extraction temperature, 1 g:38 mL for material to liquid ratio, and 185 W for ultrasonic power. Under the same operating conditions, the yield of L . barbarum polysaccharide using ultrasonic extraction was 83.3%, which was higher than that hot water extraction. Moreover, the extraction time of ultrasonic extraction was only 47% of that using hot water extraction. This suggested that there was great potentials of using ultrasonic extraction in the realization of high-efficiency extraction of L. barbarum polysaccharide. The results of this study could also provide a theoretical basis for the coupling of ultrasonic extraction and ultrasonic concentration process to develop the integrated equipment of both ultrasonic extraction and ultrasonic concentration.
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Food Chemistry-X
·
2025
·
IF 8.2
Zhifeng Zhang,Zehao Li,Gang Fan,Lulu Zhang,JingNan Ren,Kangning Wu,Youli Ma
Identifying the key aroma-active compounds in wolfberry pulp is essential for ensuring product quality. In this study, the key odorants in fresh wolfberry pulp (FWP), dried wolfberry pulp (DWP) and mixed wolfberry pulp (MWP) were systematically characterized using sensory evaluation, comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GC × GC-TOFMS), gas chromatography-mass spectrometer-olfactometry (GC–MS-O), and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS). A total of 58 odorants were detected across the three samples, exhibiting flavor dilution (FD) factors ranging from 1 to 243, while 33 odorants showed odor activity values (OAVs) greater than 1. Data analysis identified hexanal, ( E )-2-hexenal, geranylacetone, 2-undecanone, 6-methyl-5-hepten-2-one, 1-hexanol, 4-hexen-1-ol, 2-hexen-1-ol, 2-pentylfuran, eremophilene, and β-selinene as the key aroma-active compounds in FWP. Furthermore, HS-GC-IMS provided distinctive fingerprint profiles that allowed clear differentiation of 84 odorants among FWP, DWP, and MWP. These findings establish a theoretical basis for the quality control and improvement of wolfberry pulp products.
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Journal of Future Foods
·
2026
·
IF 7.2
Qian-Lan Wu,Jing Li,Tian-Le Mao,Xin Guo,Zhi-Jing Ni,Kiran Thakur,Yi-Long Ma,Zhao-Jun Wei
Lycium barbarum leaf (LBL) is a traditional Chinese vegetable rich in phenolics and with prominent health properties. However, the high bioaccessible LBL phenolics and their activity against alcoholic injury are under study. Herein, in vitro digestion/Caco-2 cell model was used to identify LBL phenolics with high bioaccessibility. In 45 identified LBL phenolics, rutin, sinapic acid, ferulic acid, quercetin, scopoletin, and 3,4-dihydroxymandelic acid (DA) displayed higher content (0.56 ∼ 7.16 μg/mL) and intestinal transport rates (7.34 ∼ 51.28%) after simulated digestion/absorption. Among these phenolics, DA was the most potent agent against alcoholic injury, elevating the viability of alcohol-injured AML-12 cells to 62.57%. By markedly reducing intracellular ROS levels by 28.39 - 45.04%, restoring redox-related indices (decreasing AST/ALT by 56.07%/33.27% and MDA by 27.26%, while enhancing SOD, CAT, and GSH activities), DA significantly alleviated the alcohol-triggered oxidative damage of AML-12 cells. Network pharmacology and molecular docking revealed that DA targeted YWHAG, regulating downstream pathways including PI3K-Akt, Foxo, and MAPK signaling pathway to exert hepatoprotective effects. These findings provide insight into the health properties of LBL and support its further utilization in functional foods.
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