FOOD CHEMISTRY
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2026
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IF 10.4
Huiling Li,Keyu Tian,Ruiqi Wang,Ruyu Shangguan,Jingna Yang,Nana Yang,Xintao Meng,Xiang Li,Ting Zhang,Hongjie Lei
Physical and functional properties of LPDFs were improved by modification methods. • Modification methods effectively increased soluble dietary fiber (SDF) content. • High-pressure homogenization (HPH) could loosen the dense structure of LPDFs. • Alkaline hydrogen peroxide (AHP) effectively enhanced thermal stability of LPDFs. • Complex enzymatic hydrolysis (CE) exhibited superior hydration capacity of LPDFs. Lycium ruthenicum pomace, a juice-processing by-product, is characterized by low soluble dietary fiber (SDF) content and limited functionality, which restricts its further application. In this study, the pomace dietary fibers (LPDFs) were modified using high-pressure homogenization (HPH), complex enzymatic hydrolysis (CE), and alkaline hydrogen peroxide (AHP), and the resulting changes in physicochemical, structural, and functional properties of both SDF and insoluble dietary fiber (IDF) fractions were evaluated. Modification treatments effectively increased SDF content (from 6.89% to 15.44%) and enhanced functional attributes. AHP produced the highest SDF yield (15.44%) and crystallinity (16.54%), and improved thermal stability. In contrast, CE and HPH treatments loosened the compact structure, reduced particle size, and increased antioxidant activity and viscosity. Notably, CE treatment yielded a substantial SDF increase (12.61%) and exhibited superior overall functional performance, including higher viscosity, rehydration capacities, and antioxidant activities. Our findings suggest that CE represents a more appropriate modification approach for LPDFs. Download: Download high-res image (264KB) Download: Download full-size image
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高影响因子
JOURNAL OF PROTEOME RESEARCH
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2018
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IF 3.95
Qi Wang,Shaohua Zeng,Xiangyu Wu,Hehua Lei,Ying Wang,Huiru Tang
Fruits of Lycium ruthenicum (LR) and L. barbarum (LB) in Solanaceae family contain abundant bioactive metabolites used widely as functional food and natural medicine. To characterize the fruit developmental molecular phenotypes, we comprehensively analyzed metabolite composition of both Lycium fruits at three developmental stages using the combined NMR, liquid chromatography–tandem mass spectrometry, and gas chromatography-flame ionization detector/mass spectrometry methods. The metabonomes of these fruits were dominated by over 90 metabolites including sugars, amino acids, tricarboxylic acid (TCA) cycle intermediates, fatty acids, choline metabolites, and shikimate-mediated plant secondary metabolites. Metabolic phenotypes of two species differed significantly at all three developmental stages; LB fruits contained significantly more sugars and amino acids but less TCA cycle intermediates, fatty acids, and secondary metabolites than LR. Interspecies differences for fatty acid levels were much greater after color-breaking than precolor-breaking. Furthermore, LR fruits contained more osmolytes than LB fruits indicating different osmoregulation requirements for these fruits during development. Significant differences were also present in biosynthesis of shikimate-mediated plant secondary metabolites in LR and LB. These findings provided essential metabolic information for plant physiology of these Lycium species and their utilizations demonstrating the usefulness of this metabonomic phenotyping approach for studying fundamental biochemistry of the plant development.
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高影响因子
JOURNAL OF FOOD SCIENCE
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2018
·Vol. 83
·期 10
·2448-2456
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IF 2.018
Xiaohui Zhao,Banmacailang Dong,Pi Li,Wei Wei,Jun Dang,Zenggeng Liu,Yanduo Tao,Hongping Han,Yun Shao,Huilan Yue
Lycium ruthenicum Murr. (LRM, Solanaceae), also called “black wolfberry” in China, is extremely tolerant drought. Although black wolfberry has seeds that are rich in oil, being considered as a potential alternative for edible oil production, little information has been found regarding its characterization. The present study evaluated the fatty acid profile of the seed oil and its phytosterol contents. Moreover, its antioxidant potential and its inhibition activities on pancreatic lipase, and cholesterol esterase (CEase) were also determined. The key fatty acids of studied oil were linoleic (74.58%), oleic (11.82%), γ -linolenic (6.60%), palmitic (4.88%), and stearic (1.40%) acids, which together comprised 99.28% of the total fatty acids. Phytosterols including 24-methylenecholesterol, campesterol, stigmasterol, dihydrolanosterol, 24-methyldesmosterol, β -sitosterol, Δ 5 -avenasterol, cycloartenol, and Δ 7− avenasterol were identified for the 1st time. The inhibition values (IC 50 ) for the DPPH and ABTS radical scavenging capacities of the oil were 7.86 and 15.49 mg/mL, respectively. Additionally, LRM seed oil was also demonstrated to possess strong pancreatic lipase, and CEase inhibition activities with IC 50 values of 12.38 and 2.63 mg/mL, respectively. The mechanism study indicated that the inhibitory manner of the oil on CEase belonged to uncompetitive inhibition of reversible inhibition. The results derived from above studies suggest that LRM seed oil could be a potential source of high value phytochemicals for developing novel functional food.
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FOOD CHEMISTRY
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2019
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IF 5.399
Xiaoman Yang,Shuang Lin,Yongxia Jia,Fazal Rehman,Shaohua Zeng,Ying Wang
The Lycium ruthenicum (Lr) fruit is a widely used nutritional food that contains various bioactive components such as anthocyanin and spermidine derivatives. In the present study, ultra-high-performance liquid chromatography with tandem mass spectrometry was utilized to profile the metabolic dynamics of four developmental stages of Lr fruit. A total of 49 compounds, including anthocyanin, alkaloids, hydroxycinnamic acid derivatives, flavonoids, and amino acids, were tentatively identified. Principal component analysis distinguished the fruit at four developmental stages using 15 (9 were tentatively identified) potential marker compounds. Pearson correlation analysis suggested that anthocyanin and spermidine derivative hexoses had a strong positive correlation coefficient. A glucosyltransferase (HG27071) was confirmed to glucosylate both anthocyanidin and spermidine derivative in vitro . Our results provide insight into the metabolic linkages among bioactive components in Lr fruits. The glucosyltransferase identified in this study will promote its potential use in functional foods and natural pigment resources.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yi-Kao Hu,Ayzohra Ablat,Xiao-Lin Bai,Xiang Yin,Chao Ma,Xun Liao
Lycium ruthenicum Murr is an important edible and medicinal plant widely cultivated in Northwest China. Our previous study found that its fruit possessed rich polyphenols exhibiting significant neuroprotective effects, inspiring us to investigate the active constituents of its rootstocks, which have never been reported. In this study, we rapidly identified four phenolamides (1–4) possessing monoamine oxidase B (MAO-B) inhibitory effect by using MAO-B-functionalized cellulose filter paper-based ligand fishing, and their structures were elucidated with NMR and MS. Compounds 1, 2, and 4 exhibited moderate inhibitory effect on MAO-B with IC50 values of 71.44 ± 1.81, 28.97 ± 1.20, and 25.58 ± 0.74 μM, respectively, and enzyme kinetic analysis, molecular docking, and molecular dynamics further revealed the inhibition mechanism. All four phenolamides displayed a neuroprotective effect against 1-methyl-4-phenylpyridinium -induced SH-SY5Y cells model, among which 2 and 4 significantly reduced intracellular MAO-B activity and improved the ATP content. Western blot revealed that compounds 2 and 4 alleviated the oxidative stress through up-regulation of the expression of Nrf2 and HO-1 protein, promoting mitophagy via activating the PINK1/Parkin pathway. Moreover, compounds 2 and 4 extended the lifespan, dopamine and ATP levels, locomotor behavior, and olfactory ability of the PINK1B9 flies, a drosophila model of Parkinson’s disease. Meanwhile, compounds 1–4 showed potent antioxidative activities on DPPH, ABTS+, and PTIO radical scavenging assays. These results revealed the health-promoting effects of the rootstocks in preventing and treating neurodegenerative diseases, laying the scientific foundation for the exploitation of this plant byproduct for the development of functional food formulations and pharmaceutical products.
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Frontiers in Sustainable Food Systems
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2022
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IF 5.005
Dong Yuhe,Zhong Wenting,Yang Chunmiao,Zhang Yongze,Yang Dongsheng
The objectives of this study are to optimize the ultrasonic microwave synergistic extraction (UMSE) of anthocyanins from Lycium ruthenicum Murr (ALRM) by response surface methodology and also to investigate its antioxidant activity in vitro. Based on the single-factor experiments, we optimized the ALRM extraction process by response surface methodology assuming anthocyanin extraction rate as the response point and microwave power, ultrasonic power, extraction time, and liquid-to-material ratio as the investigating parameters as well as performed correlation analysis between DPPH·, ·OH, ABTS·, O2-· radical scavenging potential, and ferric-reducing antioxidant power. The results showed that the influence on anthocyanin yield was in the order of liquid to material ratio > microwave power > ultrasonic power > extraction time; the elicited extraction conditions were: ultrasonic power 216.253 W, microwave power 89.311 W, time 26.141 min, liquid to material ratio 17.294 mL/g, the forecast content was 10.157 mg/g, while the actual value was consistent with the preview value. Meanwhile, ALRM obtainable by UMSE was identified as yielding favorable antioxidant properties firstly, when the concentration of purified ALRM (ALRM-1) was increased, the antioxidant capacity was strengthened. Theoretically, we provide a basis for the extraction procedure of ALRM and its antioxidant activity, which serves as a promising antioxidant and free radical scavenger.
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TISSUE & CELL
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2026
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IF 3.1
Kai Deng,Gaoyuan Zhang,Qiang Yan,Shuwen Han,Yunyao Wei,Ying Mei
LRACN alleviated acute RP by reducing lung inflammation and oxidative stress. • LRACN partially reversed thoracic irradiation-induced gut microbiota dysbiosis. • Gut microbial and metabolic remodelling was associated with improved RP-related lung phenotypes. • Multi-omics linked LRACN protection to gut–lung axis modulation and a PI3K/AKT-related signalling axis. Radiation pneumonitis (RP) is a dose-limiting complication of thoracic radiotherapy, and effective preventive interventions remain limited. Lycium ruthenicum anthocyanins (LRACN) exhibit antioxidant and anti-inflammatory activities, but their effects on RP and the associated systemic mechanisms remain unclear. In this study, a mouse RP model was established by 15 Gy localised chest irradiation, and LRACN was administered orally before and after irradiation. Protective effects were evaluated using histopathology, inflammatory cytokines, and oxidative stress indices. Potential mechanisms were explored by integrating 16S rRNA sequencing, non-targeted serum metabolomics, metabolite-based target network analysis, transcriptomics, and single-cell RNA-seq. Compared with the model group, high-dose LRACN reduced injury score, collagen volume fraction, tumour necrosis factor-α, and malondialdehyde in the lung tissue by approximately 55%, 58%, 45%, and 44%, respectively. Multi-omics profiling revealed that LRACN partially restored radiation-disrupted gut microbial taxa, including Dubosiella , Ligilactobacillus , and Akkermansia , and reversed radiation-induced disturbances in serum purine and glycerophospholipid metabolism. Correlation analysis linked LRACN-responsive gut taxa and circulating metabolites with RP-related pathological, inflammatory, and oxidative indices. Integrated pathway analysis and western blotting suggested that the protective effect of LRACN was associated with reduced PI3K and Akt phosphorylation in lung tissue. These findings indicate that LRACN mitigates early RP in mice, and gut microbiota-associated metabolic remodelling may contribute to its protective effects. Download: Download high-res image (189KB) Download: Download full-size image
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Siyuan Tang,Da Chen,Guoqiang Guo,Xiameng Li,Changxing Wang,Tingting Li,Gang Wang
Growing global environmental pollution problems challenge the need for converting biomass into an advantageous product. In this paper, Lycium ruthenicum is successfully turned into beneficial green emissive (527 nm) fluorescent nitrogen doping carbon dots (N-CDs) via the hydrothermal treatment for the first time. The horizontal and vertical dimensions of N-CDs are demonstrated to be about 4.5 and 0.73 nm, respectively. The N-CDs possess an extremely stable green fluorescence and quantum yield up to 21.8%. Meaningfully, N-CDs exhibit a good linear relationship with Ag + in the range of 0.7–36 μM, and its detection limit is determined to be 59 nM. The practicability of the fluorescent probe is further validated in lake water and the satisfactory spiked recoveries of Ag + ranges from 98.99% to 104.19%. Besides, based on the sensitive and selective photoluminescence quenching properties, a smartphone-based laboratory device and RGB analysis software are used to directly capture and analyze fluorescence images with a sensitive detection limit of 83 nM for Ag + . This novel sensor based on N-CDs and smartphone provides a reliable way for on-site monitoring of Ag + and expands application prospect in the field of environmental pollution detection.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Yi-Kao Hu,Xiao-Lin Bai,Hao Yuan,Yi Zhang,Emmanuel Ayodeji Ayeni,Xun Liao
The fruits ofLycium ruthenicum Murr have long been consumed as health food and used in folk medicine in China. Apart from the well-known polysaccharides, the active small molecular constituents in this fruit have not been fully studied. In this work, a systematic phytochemical study was carried out to investigate the small molecules and their potential health benefits. Nine new polyphenolic glycosides, lyciumserin A–I (1–9), together with 16 known compounds (10–25), were isolated and elucidated by high-resolution electrospray ionization mass spectrometry and comprehensive NMR analyses in combination with chemical hydrolysis. Compounds 1, 2, and 16 exhibited moderate inhibitory activity of monoamine oxidase B (MAO-B), while compounds 1 (50 μM) and 2 (100 μM) displayed significant neuroprotective effects (69.22 and 72.38% of cell viability, respectively) in the 6-hydroxydopamine-induced injury of the PC12 cell model (54.41%), comparable to the positive drug rasagiline (70.45%). The neuroprotective effect of 1 and 2 was further evidenced by the observation of the morphological change and fluorescein diacetate/propidium iodide staining. In addition, the levels of the major active compounds (1, 3, 5/6, and 16–18) vary from 21.5 to 892.3 μg/g. This is the first report on phenolic glycosides from the fruits ofL. ruthenicum Murr that possess both significant MAO-B inhibitory and neuroprotective effects, indicating the promising potential of the fruits for the development of health care products and even therapeutic agents for the treatment of Parkinson’s disease and other neurodegenerative diseases.
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JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 3
·696-706
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IF 2.479
Meilun Shen,Kang Liu,Yefei Liang,Guangxin Liu,Jie Sang,Cuiqin Li
The aim of this study was to extract and purify anthocyanins from Lycium ruthenicum Murr. and evaluate their tyrosinase inhibitory activity. Response surface methodology was devoted to optimize enzyme-assisted extraction of anthocyanins from L. ruthenicum dried fruits. Extraction at 38 °C for 37 min using water-containing pectinase (52.04 mg/100 g dried fruit) rendered an anthocyanin extraction yield of 19.51 ± 0.21 mg/g. The purified anthocyanins were separated from the extract by macroporous resin XDA-6. Antioxidant tests in vitro suggested that the extract and the purified anthocyanins exhibited a potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging capacity, hydroxyl radical scavenging capacity, superoxide radical scavenging capacity, and total reducing power. Thirteen anthocyanins from L. ruthenicum dried fruits were analyzed by HPLC-MS. Moreover, the purified anthocyanins had inhibitory effect on tyrosinase monophenolase (IC 50 = 1.483 ± 0.058 mg/mL), and the type of inhibition was competitive inhibition ( K i = 39.83 ± 1.4 mg/mL). The maximum inhibitory activity of the purified anthocyanins (3.00 mg/mL) on tyrosinase diphenolase was 42.16 ± 0.77%, and the type of inhibition was anticompetitive inhibition ( K is = 2.387 ± 0.10 mg/mL). Practical Application The anthocyanins from L. ruthenicum dried fruits can be used as tyrosinase inhibitors in medicine, cosmetics, and food preservation industries.
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Journal of Functional Foods
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2017
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IF 3.144
Jilong Tang,Yamei Yan,Linwu Ran,Jia Mi,Yi Sun,Lu Lu,Yuxiao Gao,Xiaoxiong Zeng,Youlong Cao
The fruit of Lycium ruthenicum Murray has been widely used as a precious Chinese herbal medicine. In this study, the major anthocyanin in L. ruthenicum fruit was obtained via an AKTA purifier system with YMC-Pack ODS-A column and identified by mass and nuclear magnetic resonance spectroscopy to be petunidin 3- O -[6- O -(4- O -( trans - p -coumaroyl)-α- l -rhamnopyranosyl)-β- d -glucopyranoside]-5- O -[β- d -glucopyranoside] (compound 1 ). Compound 1 exhibited higher scavenging activities against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 1,1-diphenyl-2-picrylhydra-zyl and superoxide radicals than the crude extract of anthocyanins. Moreover, compound 1 was demonstrated to have potential cytoprotective effect on hydrogen peroxide-induced intracellular reactive oxygen damage in neuronlike rat pheochromocytoma line cells by promoting cell proliferation, mitigating lipid peroxidation and regulating endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase).
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shihai Zhang,Fei He,Xia Chen,Kan Ding
Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose ™ Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Gal p , 1,3-linked β-D-Gal p , 1,6-linked β-D-Gal p , 1,3,6-linked β-D-Gal p , 1,5-linked α-L-Ara f , 1,3,5-linked α-L-Ara f , T-linked α-L-Ara f and T-linked β-D-Xyl p . Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.
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