AQUACULTURE
·
2026
·
IF 4.4
Kaige Song,Enpei Zhu,Fuqi You,Xiang Fan,Chengxian Zhang,Hanwen Zhang,Xue Du,Qiao Liu,Liulan Zhao,Song Yang
Micropterus salmoides rhabdovirus (MSRV), a highly lethal and contagious pathogen, has inflicted substantial economic losses on the largemouth bass ( Micropterus salmoides ) aquaculture industry. In this study, a novel MSRV strain, designated MSRV-MS01, was isolated from diseased juvenile largemouth bass collected from Sichuan Province, China. To explore host-virus interactions, we focused on the viral glycoprotein (G), a major envelope protein involved in virus-host interactions, and performed protein–protein interaction screening to identify host-associated proteins. Prohibitin (PHB) was identified as a host interacting protein of MSRV-G protein. Functional analyses, including PHB overexpression and antibody-blocking assays, further suggested that PHB is involved in MSRV infection and may modulate viral replication. Collectively, these findings expand the current understanding of MSRV-host interactions and provide new insights into host factors associated with rhabdovirus infection.
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高影响因子
Viruses-Basel
·
2022
·Vol. 14
·期 7
·1421
·
IF 5.818
Yingjie Jin,Fei Yang,Gengrong Zhang,Qing Yu,Gaoxue Wang,Fei Ling,Tianqiang Liu
Micropterus salmoidesrhabdovirus (MSRV) is a primary viral pathogen in largemouth bass aquaculture, which leads to tremendous economic losses yearly. Currently, there are no approved drugs for the treatment and control of this virus. Our previous studies screened the herbMagnolia officinalisfrom many traditional Chinese medicines, and we isolated and identified magnolol as its main active compound against multiple rhabdoviruses, including MSRV. On the basis of the structure–activity relationship and pharmacophore model of magnolol, two new magnolol derivatives, namely, hydrogenated magnolol and 2,2′-dimethoxy-magnolol, were designed and synthesized. Their anti-MSRV activities were systematically investigated both in vitro and in vivo. By comparing the half-maximal inhibitory concentration (IC50), it was found that hydrogenated magnolol possessed a higher anti-MSRV activity than magnolol and 2,2′-dimethoxy-magnolol, with an IC50of 13.37 μM. Furthermore, hydrogenated magnolol exhibited a protective effect on the grass carp ovary (GCO) cell line by reducing the cytopathic effect induced by MSRV. Further studies revealed that hydrogenated magnolol did not directly impact virions or interfere with MSRV adsorption. It worked within the 6–8 h of the phase of virus replication. In vivo treatment of MSRV infection with magnolol and hydrogenated magnolol showed that they significantly improved the survival rate by 44.6% and 62.7%, respectively, compared to MSRV-infected groups. The viral load measured by the expression of viral glycoprotein in the organs including the liver, spleen, and kidney also significantly decreased when fish were intraperitoneally injected at a dose of 20 mg/kg. Altogether, the structural optimization of magnolol via hydrogenation of the propylene groups increased its anti-MSRV activity both in vitro and in vivo. These results may provide a valuable reference for anti-MSRV drug discovery and development in aquaculture.Keywords:Micropterus salmoidesrhabdovirus (MSRV);magnolol;largemouth bass;antiviral activity
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高影响因子
Animals
·
2026
·Vol. 16
·期 13
·2032
·
IF 3.2
Jing Ni,Hejian Xiong,Ruifang Wang,Yuanhong Xie,Lixing Huang,Ying Ma,Chuanbo He
Simple SummaryCurcumin holds great potential as a natural immunostimulant in animal nutrition, yet its practical application is severely constrained by low water solubility and poor bioavailability. We previously synthesized a highly water-soluble valine-curcumin conjugate (Val-Cur). This study now presents the first comprehensive in vivo evaluation of Val-Cur in largemouth bass, systematically revealing its enhanced efficacy and underlying physiological mechanisms. We found that dietary Val-Cur at doses as low as 15–30 mg/kg outperforms native curcumin (60 mg/kg) across multiple key metrics: it more effectively promotes growth, improves serum lipid profiles, and strengthens systemic antioxidant and anti-inflammatory responses. These benefits are achieved through a coordinated mechanism that includes reinforcing intestinal barrier integrity, modulating apoptosis-related gene expression, and beneficially reshaping the gut microbiota—specifically by inhibiting potential pathogens while enriching beneficial, short-chain fatty acid-producing bacteria. The poor water solubility of curcumin is a major bottleneck in animal nutrition. Our findings demonstrate that an amino acid conjugation strategy effectively reduces the effective dose while surpassing native curcumin in efficacy, representing an important step for functional feed development.Curcumin (Cur), a polyphenol with excellent antioxidant and anti-inflammatory properties, is widely used in aquaculture. However, its low water solubility limits bioavailability. This study first investigated the effects of a highly water-soluble and bioavailable valine-curcumin (Val-Cur) on growth performance, antioxidant capacity, inflammatory factor expression and gut microbiota in juvenile largemouth bass. A total of 450 healthy largemouth bass (initial weight 12.00 ± 0.45 g per fish) were randomly divided into six groups: the basal diet group (CK), the group supplemented with 60 mg/kg of curcumin (Cur), and groups that were fed with 15, 30, 60, and 120 mg/kg of Val-Cur. Each group had three replicates (25 fish per replicate). After 8 weeks, compared with both the CK and Cur groups, 30–60 mg/kg Val-Cur displayed significantly increased growth rates, specific growth rates, and protein conversion efficiency, and significantly decreased the feed conversion ratio. Quadratic regression analysis indicated that the optimal supplementation level was approximately 51.62 mg/kg Val-Cur. The Cur and Val-Cur between 30 and 60 mg/kg groups also displayed significantly improved serum biochemical indicators (↑HDL-C, ALB, LZM, CAT and SOD, and ↓MDA). These groups also promoted the expression of intestinal anti-inflammatory factors (TGF-β1andIL-10) and physical barrier genes (Claudin1,OccludinandClaudin4). In terms of regulating the intestinal microbiota, both Cur and Val-Cur significantly reduced the bacterial diversity (↓Sobs, Chao1 and PD indices) and increased the evenness of bacterial distribution (↑Simpson, Shannon and Pielou indices). In addition, the abundance of some potential pathogens (e.g.,Plesiomonas,Plesiomonas shigelloides, andPseudomonas fluorescens) significantly decreased, while the abundance of beneficial bacteria (e.g.,Faecalibaculum,Faecalibaculum rodentiumandLactobacillus murinus) significantly increased. Pearson’s correlation analysis showed that the increase in the abundance of beneficial bacteria was positively correlated with improvement in growth performance, serum biochemical and antioxidant capacity indicators. In summary, Val-Cur exerted superior biological effects at lower dietary inclusion levels than Cur. This study laid a theoretical foundation for elucidating the mechanism of Val-Cur in improving fish immunity and promoting the application of water-soluble curcumin in aquaculture.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 3
·582-591
·
IF 2.479
Luyun Cai,Jiangli Wan,Xiuxia Li,Jianrong Li
To evaluate the physicochemical properties and structure of thawed fillets, following six treatments were used: conventional thawing, microwave thawing, microwave or ultrasound combined with vacuum thawing, magnetic nanoparticles combined with microwave or far-infrared thawing. The thawing loss, cooking loss, pH, color, texture change, water-holding capacity, and water migration of fish fillets were determined. The total volatile base nitrogen and thiobarbituric acid were used to determine the degree of protein degradation and lipid oxidation. Microscope observations and scanning electron microscope (SEM) were used to observe fiber microstructure. Results indicated that both microwave combined with vacuum thawing and far-infrared combined with magnetic nanoparticles thawing had desirable physicochemical properties as compared to other thawing methods. The lower total volatile base nitrogen and thiobarbituric acid values had less effect on protein degradation and lipid oxidation of thawing process. Besides, both microwaves combined with vacuum thawing and far-infrared combined with magnetic nanoparticles thawing samples had no significant difference in immobilized and bond water compared with fresh sample. SEM and microscopic observation showed that the myofibril bundles were arranged regularly and smoothly in microwave combined with vacuum thawing and far-infrared combined with magnetic nanoparticles thawing samples compared with other thawed methods. Thus, the microwave combined with vacuum thawing and far-infrared combined with magnetic nanoparticles thawing as potential thawing methods could be used to maintain the quality of thawed fish fillets. Practical Application Largemouth bass ( Micropterus salmoides ) is a good source of animal protein. The fish needs to be frozen for circulation because of geographical and seasonal factors, so thawing methods can directly affect the quality of thawed fish. The results showed that the microwave combined with vacuum and the magnetic nanoparticles combined with far-infrared thawing could better maintain the quality of thawed fish.
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高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Han Mengyao,Zhang Zhongshan,Li Xinyue,Tong Haibin,Xu Zhiguo,Ding Zikang,Yang Anquan,Xie Min,Wang Xiaomei
To investigate the protective effect of collagen peptide from Micropterus salmoides skin (CPMs) on oxidative damage induced by cyclophosphamide in mice. Balb/c female mice were divided into blank, model (cyclophosphamide, CTX), positive control (levamisole hydrochloride), and collagen peptide low-, medium-, and high-dose groups. The results showed that CPMs increase the body mass and immune-related organ indexes, such as liver and kidneys of immunosuppressed mice. The activities of ALT, AST, UA, BUN, and MDA in the liver and kidney tissues decreased significantly, while those of SOD and GSH-Px increased significantly. CPMs can relieve the pathological damage to immune organs. CPMs significantly increase the activities of IL-2, IgG, and TNF-α in serum and SOD activity, while the MDA content was decreased compared to the model group. CPMs can exert a protective effect on cyclophosphamide-induced oxidative damage and have application prospects in the field of health food.
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PubMed
高影响因子
JOURNAL OF FISH DISEASES
·
2022
·Vol. 45
·期 11
·1599-1607
·
IF 2.58
Xin-Yuan Jiang,Zong-Yi Ren,Nian-Kun Zhang,Ke-Chen Yang,Gao-Xue Wang,Hai-Feng Jiang
Nocardiosis caused by Nocardia seriolae is a major threat to the aquaculture industry. Given that prolonged therapy administration can lead to a growth of antibiotic resistant strains, new antibacterial agents and alternative strategies are urgently needed. In this study, 80 medicinal plants were selected for antibacterial screening to obtain potent bioactive compounds against N. seriolae infection. The methanolic extracts of Magnolia officinalis exhibited the strongest antibacterial activity against N. seriolae with the minimal inhibitory concentration (MIC) of 12.5 μg/ml. Honokiol and magnolol as the main bioactive components of M. officinalis showed higher activity with the MIC value of 3.12 and 6.25 μg/ml, respectively. Sequentially, the evaluation of antibacterial activity of honokiol in vivo showed that honokiol had good biosafety, and could significantly reduce the bacterial load of nocardia-infected largemouth bass ( p < .001). Furthermore, the survival rate of nocardia-infected fish fed with 100 mg/kg honokiol was obviously improved ( p < .05). Collectively, these results suggest that medicinal plants represent a promising reservoir for discovering active components against Nocardia , and honokiol has great potential to be developed as therapeutic agents to control nocardiosis in aquaculture.
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高影响因子
International Journal of Gastronomy and Food Science
·
2019
·
IF 0
Jiangli Wan,Ailing Cao,Luyun Cai
In order to estimate the effects of vacuum (Vacuum boiling (VB), Vacuum steaming (VS)) or sous-vide cooking (SV) methods on the quality of largemouth bass compared with traditional cooking methods (Boiling (BT), Steaming (ST)), the pH, cooking loss, color and texture indicator were used to evaluate physicochemical changes. The thiobarbituric acid (TBA) was used to analyze lipid oxidation. The low-field nuclear magnetic resonance (LF-NMR) was used to explore the water migration and distribution. The scanning electron microscopy (SEM) was used to observe the microstructure. Raman spectroscopy was carried out to determine the structural changes. The results showed that the VS and SV samples had desired quality, showing more stable protein secondary structure and lower lipid oxidation. In addition, the VS and SV samples had more regular and smooth myofibril bundles compared with other cooked samples. Thus, the VS and SV cooking methods could be used to better maintain the quality of fillets.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Maosheng He,Ying Yan,Xiang Liu,Linhan Li,Bin Yang,Mingzhu Liu,Qing Yu,Erlong Wang,Pengfei Li,Tianqiang Liu,Gaoxue Wang
Diseases caused by pathogens severely hampered the development of aquaculture, especially largemouth bass virus (LMBV) has caused massive mortality and severe economic losses to the culture of largemouth bass ( Micropterus salmoides ). Considering the environmental hazards and human health, effective and environmentally friendly therapy strategy against LMBV is of vital importance and in pressing need. In the present study, a novel nanobody (NbE4) specific for LMBV was selected from a phage display nanobody library. Immunofluorescence and indirect ELISA showed that NbE4 could recognize LMBV virions and had strong binding capacity, but RT-qPCR evidenced that NBE4 did not render the virus uninfectious. Besides, antiviral drug ribavirin was used to construct a targeted drug system delivered by bacterial nanocellulose (BNC). RT-qPCR revealed that NbE4 could significantly enhance the antiviral activity of ribavirin in vitro and in vivo . The targeted drug delivery system (BNC-Ribavirin-NbE4, BRN) reduced the inflammatory response caused by LMBV infection and improved survival rate (BRN-L, 33.3 %; BRN-M, 46.7 %; BRN-H, 56.7 %)compared with control group (13.3 %), ribavirin group (RBV, 26.7 %) and BNC-ribavirin (BNC-R, 40.0 %), respectively. This research provided an effective antiviral strategy that improved the drug therapeutic effect and thus reduced the dosage.
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高影响因子
Aquaculture Reports
·
2025
·
IF 3.7
Huifen Liu,Yueying Wang,Jiaying Fang,Meng Zhang,Xi Shi,JianXin Zhang,Ruwei Xu,Xuejun Li,Xianghui Kong
Temperature plays a crucial role in the gonadal development and maturation of fish. However, the mechanism by which fish perceive temperature signals and respond accordingly, thereby altering their developmental trajectory, remains incompletely understood. In this study, the largemouth bass (LMB) broodstock were reared at ambient temperature, 18℃, 23℃ and 28℃ for 60 days respectively. The growth differences, antioxidant capacity, serum hormones and ovarian development were compared, and comparative transcriptome analyses of ovarian tissues were performed. The results revealed that the weight gain (WG, %), specific growth rate (SGR, %/day), and gonadosomatic index (GSI, %) of LMB at 18℃ were significantly higher than those in other groups ( p < 0.05). Compared to the control group under natural water temperature, fish maintained at constant temperatures (18 °C, 23 °C and 28 °C) exhibited significantly higher activities of total antioxidant capacity (T-SOD) and catalase (CAT), along with a markedly lower malondialdehyde (MDA) content. Histological analysis showed that under 18℃ and 23℃ conditions, most oocytes were in the vitellogenic stage, while oocytes in the 28℃ group were mainly in the primary growth stage and cortical vesicle stage, with some oocytes atretic. Additionally, transcriptome sequencing detected a total of 30,523 genes, and comparative analysis found 854, 1344, 1823, 322, 1400, and 1349 DEGs in 18℃ vs control, 23℃ vs control, 28℃ vs control, 23℃ vs 18℃, 28℃ vs 18℃, and 28℃ vs 23℃, respectively. KEGG analysis revealed that these DEGs were mainly enriched in pyruvate metabolism, thermogenesis, apelin signaling pathway, PPAR signaling pathway, GnRH signaling pathway, ovarian steroid biosynthesis, PI3K-AKT, TGF-β signaling pathway, prolactin signaling pathway. The results can provide a reference for a deeper understanding of the molecular mechanism by which temperature regulates the gonadal maturation of fish, and also contribute to the analysis of the mechanism of environment-gene interaction.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 4.3
Zhang Zhenyang,Chen Weijun,Shen Jiaqi,Dong Haowei,Zhang Chunnuan,Gao Xiaochan,Ren Hongtao,Huang Yong,Gao Shiyang
Waterborne cadmium (Cd) remains a significant threat to the intestinal health and growth of largemouth bass ( Micropterus salmoides ), necessitating the search for effective dietary mitigants. In this 56-day trial, the protective efficacy of paeonol (PAE) was evaluated across five experimental groups: a negative control, a Cd-stressed group (Cd, 2.75 mg/L waterborne exposure), and three treatment groups concurrently exposed to 2.75 mg/L Cd and supplemented with dietary PAE at 100, 200, and 400 mg/kg, respectively. The results showed that Cd exposure induced intestinal oxidative stress, impaired structural integrity, downregulated antimicrobial peptide expression, compromised immune function, and disrupted intestinal microbial homeostasis ( P < 0.05). PAE supplementation significantly decreased malondialdehyde (MDA) content, and the PAE200-Cd exhibited increased activities of total superoxide dismutase (T-SOD) and catalase (CAT), as well as elevated expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and cytochrome P450 1A1 (CYP1A1) ( P < 0.05). Intestinal villus structure was restored, the number of goblet cells increased, and the expression of tight junction proteins (Occludin, ZO-1, Claudin-1) genes was significantly upregulated ( P < 0.05). Meanwhile, the expression of β-defensin, Lysozyme, and Mucin 2 was significantly upregulated ( P < 0.05). The PAE100-Cd showed increased expression of immunoglobulins (e.g., IgM and IgT), while the PAE200-Cd inhibited the expression of pro-inflammatory factors ( P < 0.05). Cd-induced negative changes in intestinal microbial diversity and stability were restored by PAE, and the PAE200-Cd exhibited decreased abundances of Proteobacteria and Pseudomonas , and increased abundance of Firmicutes ( P < 0.05). Under the present experimental conditions, the appropriate dietary PAE level based on oxidative stress and intestinal physical barrier was preliminarily estimated to be 115.51–229.55 mg/kg. Collectively, PAE can alleviate Cd-induced intestinal injury by improving antioxidant capacity and enhancing intestinal barrier function, and represents a promising functional candidate for heavy metal mitigation. Graphical The alternative text for this image may have been generated using AI.
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AQUACULTURE NUTRITION
·
2021
·Vol. 27
·期 6
·2749-2758
·
IF 3.497
Yan-Yu Zhang,Jun-Xian Wang,Jiong Ren,Jie Wang,Fang Qiao,Mei-Ling Zhang,Zhen-Yu Du
In aquaculture, excess lipid deposition in liver is a health damage in largemouth bass ( Micropterus salmoides ). Activation of peroxisome proliferator-activated receptor-α (PPARα) has been proved to stimulate lipid catabolism and alleviate fatty liver in rodents and human. Our present study was conducted to investigate the effects of Pparα activation via dietary fenofibrate stimulation on hepatic lipid catabolism in largemouth bass. Total 240 fish (10.7 ± 0.2 g) were randomly divided into eight tanks and fed two diets (control and control containing 5 g/kg fenofibrate), respectively, for 4 weeks. Dietary fenofibrate increased Pparα protein and mitochondrial DNA amounts. The weight gain rate and carcass ratio of fish with Pparα activation decreased, but viscerosomatic index and hepatosomatic index were higher in the fenofibrate-fed fish than the control. Pparα activation significantly reduced triglyceride levels in the serum and liver but did not change the lipid composition in liver. Moreover, hepatic glycogen accumulation was found in the fish with Pparα activation. The Pparα activation also had health benefits for liver, as reflected by reducing serum ALT and AST activities, and increasing antioxidative ability in liver. Our results showed that in largemouth bass, Pparα can be activated and the Pparα activation could efficiently stimulate lipid catabolism and reduce hepatic fat deposition, and promote health in liver.
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AQUACULTURE
·
2023
·
IF 4.5
Xiangjie Mao,Wangwang Chen,Xianmei Long,Xiaomei Pan,Guoqing Liu,Wenguang Hu,Dianchao Gu,Qingsong Tan
The aim of this study was to investigate the effects of dietary iron (Fe) levels on the growth performance, health status, meat quality, and intestinal flora of juvenile largemouth bass ( Micropterus salmoides ) and to determine the dietary Fe requirement of largemouth bass . Largemouth bass was fed with 6 isonitrogenous and isocaloric experimental diets containing Fe levels of 42.26, 50.79, 66.61, 80.86, 123.13, and 201.87 mg/kg for 10 weeks. The results showed that both final body weight (FBW) and specific growth rate (SGR) tended to increase and then decrease with increasing dietary Fe level, reaching their highest values of 50.34 g and 3.58%/d at a dietary Fe concentration of 80.86 mg/kg respectively. Feed efficiency (FE) and protein efficiency ratio (PER) increased linearly with increasing dietary Fe level. Total serum protein (TP) and urea nitrogen (UN) decreased linearly with increasing dietary Fe level. Serum superoxide dismutase (SOD) and catalase (CAT) enzyme activities tended to increase and then decrease with increasing dietary Fe level, while serum malondialdehyde (MDA) level tended to decline and then increase. In addition, increased dietary Fe level tended to improve muscle hardness and chewiness , thus improving meat quality. Analysis of the intestinal flora showed that the abundance of microorganisms such as Plesiomonas , Peptostreptococcaceae , and Lactococcus in the genus level increased significantly at dietary Fe concentrations of 80.86 mg/kg and 201.87 mg/kg, indicating that appropriate levels of dietary Fe can increase the diversity of intestinal microorganisms . Optimal dietary Fe levels for juvenile largemouth bass were determined to be between 73.81 and 83.22 mg/kg based on the broken-line regression analyses of SGR, FE, or hemoglobin content.
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