ANALYTICAL BIOCHEMISTRY
·
2025
·
IF 2.5
Zi-qing Wang,Li-ping Hao,Zi-xuan Meng,Hao-ran Zhang,Wei-jun Kang,Lian-feng Ai
An LC-MS/MS method was developed and validated for simultaneously quantifying 25-hydroxyvitamin D2, D3, and 1α,25-dihydroxyvitamin D3 in human serum. Protein in 200 μL serum was precipitated with acetonitrile. After centrifugation, the metabolites were derivatized using (diacetoxyiodo)benzene (PIA) and 4-(4-dimethylaminophenyl)-1,2,4-triazolidine-3,5-dione (DMAT) and then quantified by the LC-MS/MS system. The limits of detection (LODs) for the three substances were 10, 10, and 5 pg/mL, and the limits of quantification (LOQs) were 20, 20, and 10 pg/mL. The standard curves for these compounds showed linear regression coefficients (R2)>0.998 over specific concentration ranges. Recoveries were 94.36 %–102.34 % for 25(OH)D2, 92.43 %–103.41 % for 25(OH)D3, and 88.98 %–94.36 % for 1α,25(OH)2D3. The mean serum levels in 109 subjects (consisting of 61 healthy adult males and 59 healthy adult females) were 2.0 ± 1.5 ng/ml (25(OH)D2), 16.4 ± 6.1 ng/ml (25(OH)D3) and 36.6 ± 15.1 pg/ml (1α, 25(OH)2D3).Derivatization of vitamin D metabolites using PIA and DMAT is useful for rapidly determining the serum 25(OH) D2, 25(OH) D3 and 1α,25(OH)2D3 concentrations simultaneously in human serum.
研究领域:
分析化学
·
临床医学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2021
·
IF 6.024
Jun Zhang,Shuai Shao,Desen Zhou,Tingmin Di,Tielin Wang
Two-dimensional (2D) layered nanocomposite provides a surface-to-surface heterojunction, which is an effective way to improve the photocatalytic performance because larger contact areas can promote the separation rate of interfacial charge carriers. In this study, Co(OH)2/g-C3N4/Ni(OH)2 2D layered photocatalysts have been successfully synthesized through a simple precipitation–hydrothermal method. The composite achieves a high H2-production rate of 899 μmol·h–1·g–1, which exceeds the rate of samples only coupled with Co(OH)2 and Ni(OH)2 by 4.0 and 1.9 times, respectively. In addition, the activity is even higher than that of the optimized Pt-g-C3N4 sample (1 wt. % Pt–C). The characterization results reveal the close contact of g-C3N4 and Ni/Co hydroxides, and such a face-to-face contact can greatly improve the interfacial charge-transfer rate, resulting in a high photocatalytic performance. This work demonstrates a simple way to enhance the photocatalytic H2 production of g-C3N4 under the cooperative effect of Co(OH)2 and Ni(OH)2 and provides novel insights into constructing other 2D ternary heterostructure nanocomposites.
研究领域:
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Danyu Lv,Wanjun He,Wei Liu,Yuqiang Cheng,Yan Cui,Xiujuan Zhou,Yanwen Xue,Siming Yu,Ningjin Zhang,Hecheng Meng,Yongguang Guan,Jian-He Sun,Xian-Ming Shi
The γ-cyclodextrin (γ-CD) metal–organic frameworks (CD-MOF-1) consist of γ-CD and potassium (K+) ions through coordinating an eight-coordinated K+ ion with two C5-linked oxygen and C6-linked hydroxyl (C5–O/C6–OH) groups in the primary faces of adjacent γ-CD units and two C2- and C3-linked hydroxyl (C2–OH/C3–OH) groups in the secondary faces. Herein, we found polysaccharide gels with only C2–OH/C3–OH or C5–O/C6–OH groups in pyranoid rings can form four-coordinated K+ ions and then coordinate γ-CD in a KOH solution for CD-MOF-1 growth. Exposure of C2–OH/C3–OH or C5–O/C6–OH groups in polysaccharide gels is important to form active four-coordinated K+ ions. Mechanism supporting this work is that four-coordinated K+ ion sites are first formed after coordinating C2–OH/C3–OH groups in pectin and then coordinating C5–O/C6–OH groups in the primary faces of γ-CD units. Alternatively, four-coordinated K+ ions with C5–O/C6–OH groups in chitosan can coordinate the C2–OH/C3–OH groups in the secondary faces of γ-CD units. Mechanism of CD-MOF-1 growing on pectin and chitosan gels through the proposed four-coordinated K+ ions is also universally applicable to other polysaccharide gels with similar C2–OH/C3–OH or C5–O/C6–OH groups such as alginate gel. Based on this mechanism, we developed pectin and chitosan gel-based CD-MOF-1 composites and exemplified applications of them in antibacterial and organic dye removal. To help future research and applications of this mechanism, we share our theoretical assumption for further investigations that any matrices with an ortho-hydroxyl carbon chain or ortho-hydroxyl ether structures may form four-coordinated K+ ions for CD-MOF-1 growth. The proposed mechanism will broaden the development of novel CD-MOF-1 composites in various fields.
研究领域:
材料科学
·
化学
·
生物材料
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Yulan Song,Cuilan Tang,Tao Wang,Yansong Liu,Xiaoshan He,Chunping Xie,Guo Chen,Chengfu Deng,Zhibing He,Jinglin Huang
Constructing electronic structures with abundant surface/interface structures and effective regulation of catalytic active centers is an effective strategy to improve electrocatalytic performance, but designing efficient bifunctional non-noble metal oxygen evolution reaction (OER) and urea oxidation reaction (UOR) electrocatalysts remains challenging. Studies have shown that p-n heterojunctions can effectively regulate the electronic structure of catalytic active sites while having abundant surface/interface structure, which plays an important role in improving the native catalytic activity. Herein, FeNi-OH/Co(OH) 2 /NF p-n heterojunction structure was constructed by a simple electrochemical deposition method, and the effects of content, deposition time, and number of cycles on catalytic performance were investigated. The results show that Fe(0.02 M)Ni(0.07 M)–OH(100 s)/Co(OH) 2 (5)/NF composite material has the best OER and UOR catalytic performance. For OER, the electrocatalyst only requires an overpotential of 271 mV to obtain a current density of 50 mA cm −2 , and an applied voltage of 1.56 V to obtain a total water decomposition of 10 mA cm −2 (FeNi-OH/Co(OH) 2 /NF||Pt). For UOR, a voltage of only 1.314 V ( vs. RHE) is required to achieve a current density of 10 mA cm −2 , and an applied voltage of 1.44 V can achieve a current density of 10 mA cm −2 . Experimental, in situ measurements and theoretical results indicate that the improvement in catalytic activity is mainly due to the potential difference between FeNi-OH and Co(OH) 2 , which induces self-driven charge transfer at the interface, promoting the adsorption of reactant molecules, optimizing reaction barriers, accelerating chemical bond cleavage, and thus promoting catalytic reactions.
研究领域:
材料科学
·
电化学
·
催化
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Lv Jiabao,Cao Ang,Zhong Yunhao,Lin Qingyang,Li Xiaodong,Wu Hao Bin,Yan Jianhua,Wu Angjian
Renewable-driven electrocatalytic nitrate conversion offers a promising alternative to alleviate nitrate pollution and simultaneously harvest green ammonia. However, due to the complex proton-electron transfer processes, the reaction mechanism remains elusive, thereby limiting energy efficiency. Here, we adopt Ni(OH)₂ as a model catalyst to investigate the dynamic evolution of the reaction interface. A proposed OH cycle mechanism involves the formation of a locally OH-enriched microenvironment to promote the hydrogenation process, which is identified through in-situ spectroscopy and isotopic labelling. By further activating the dynamic state through the implementation of surface vacancies via plasma, we achieve a high Faradaic efficiency of almost 100%. The activated interface accelerates the OH cycle by enhancing dehydroxylation, water dissociation, and OH adsorption, thereby promoting nitrate electroreduction and inhibiting hydrogen evolution. We anticipate that rational activation of the dynamic interfacial state can facilitate electrocatalytic interface activity and improve reaction efficiency.
研究领域:
电化学
·
催化
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·
IF 2.6
Li Gang,Ren Haifang,Geng Chunping,Wu Xiang
Electrical energy storage devices (EES) play an important role in the development of portable electronic devices, power grids, and electric vehicles. Supercapacitors (SCs) become the focus of the EES field due to their high power density and high energy density. ZnCo 2 O 4 ternary metal oxides show considerable potential as electrodes for SCs. In this work, Ni(OH) 2 and PPy materials are combined on the surface of ZnCo 2 O 4 samples. The synthesized products possess a specific capacitance of 1256 C/g at 1 A/g and an energy density of 69 Wh/kg at 1350 W/kg. Moreover, the assembled device retains 81% of its capacity after being fully charged and discharged 10,000 times.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xisong Feng,Mengjian Gao,Wanqing Wei,Wei Song,Jian Wen,Hongyu Wang,Guipeng Hu,Xiaomin Li,Cong Gao,Jing Wu
25-hydroxyvitamin D3 (25(OH)VD3) is an important daily nutritional supplement, and direct enzymatic C25 hydroxylation of vitamin D3 (VD3) to 25(OH)VD3 is a green and sustainable method. However, the low catalytic activity and electron transfer efficiency of redox partners of cytochrome P450 limited its production. Here, structure-guided semirational design of CYP109E1 led to the mutant CYP109E1M2, which showed a 2-fold increase in 25(OH)VD3 production compared to the wild-type. Furthermore, the novel reductase domain BmRD was first employed to construct a fusion protein with CYP109E1M2. Notably, truncating only two amino acids at the N-terminus of BmRD generated a fusion protein Chimera-2, resulting in a 38.5% increase in 25(OH)VD3 production. Under optimized conditions, the engineered strain Escherichia coli 03-2 produced 491.3 mg/L 25(OH)VD3 with a conversion of 49.0% and a space-time yield of 61.4 mg/(L·h). This work demonstrates the modification and optimization potential of P450 and lays a theoretical foundation for the industrialization of the 25(OH)VD3 biosynthesis.
研究领域:
生物化学
·
分子生物学
·
酶工程
✓ 已验证
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Huiqin Ma,Zhuxiang Mao,Wen Liu,Yahao Zhao,Peng Liu,Difu Zhan,Qian Fu,Yizhong Huang,Changcun Han
ZnIn2S4 (ZIS) is an N-type direct bandgap semiconductor material with a visible light response, which exhibits excellent photocatalytic water-splitting performance. To enhance ZIS-based photocatalysts, heterojunction construction and cocatalyst loading methods have been explored extensively. In this research, a Co9S8/ZnIn2S4 (CS/ZIS) heterojunction was successfully synthesized via a solvothermal method, and then Ni(OH)2 nanoparticles were successfully loaded on CS/ZIS by photodeposition, yielding the Ni(OH)2/CS/ZIS photocatalyst. Our findings reveal tight coupling of ZIS nanosheets with the CS surface, establishing an effectively constructed heterojunction structure that enhances interface charge transfer and light absorption. The synergistic effect of CS and Ni(OH)2 significantly boosts the photocatalytic hydrogen production of ZIS composites. The optimum hydrogen production rate of CS/ZIS is 10.06 mmol·h–1·g–1 and that of Ni(OH)2/CS/ZIS reaches 12.92 mmol·h–1·g–1, a 11.9-fold increase over pure ZIS, with exceptional light stability. Furthermore, we discuss the photocatalytic reaction mechanism based on the experimental and characterization results. This work presents a novel approach to designing highly efficient photocatalysts using ZnIn2S4, aimed at enhancing the conversion of solar energy into chemical energy.
研究领域:
材料科学
·
能源化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
IEEE TRANSACTIONS ON DIELECTRICS AND ELECTRICAL INSULATION
·
2022
·Vol. 30
·期 1
·230-237
·
IF 2.509
Bin Du,Yuxiang Mai,Nanqing Chen,Yushun Zhao
The interfacial effect of nanoparticles can effectively regulate electron migration in epoxy composites. In this article, hydroxylated C60 [C60(OH)] was prepared and added to epoxy resin (EP). The inhibition of electron motion in epoxy composites was realized, and the dielectric strength of the epoxy composites was improved. The results of dynamic mechanical analysis (DMA) showed that C60(OH) regulated the structure of the crosslinked EP network and increased the crosslink density by 16.5%. The results of a density functional theory (DFT) simulation showed that the doping of C60(OH) formed a potential energy barrier at the C60(OH)-EP interface, which was conducive to the directional migration and capture of electrons driven to C60(OH). Electrostatic force microscopy (EFM) and pulsed electroacoustic (PEA) experiments showed that C60(OH) had a strong ability to capture charge, and the charge migration rate of C60(OH)/EP was 43% lower than that of pure EP in the presence of 10 kV/mm when the EP was doped with 0.1wt% C60(OH). The breakdown strength and volume resistivity increased by 17.87% and 222%, respectively. Thus, this article provides a new method to improve the dielectric strength of epoxy composites.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
Battery Energy
·
2024
·
IF 9
Xiangchao Xu,Fen Qiao,Yanzhen Liu,Wenjie Liu
Cu(OH) 2 has the advantages of ease of structural regulation, good conductivity, and relatively low cost, making it a suitable candidate material for use as an electrocatalyst. However, its catalytic efficiency and stability still need to be improved further. Therefore, Cu(OH) 2 /Cu 2 S was successfully prepared on copper foam (CF) using the in situ growth and hydrothermal method. The structural characterization showed that sulfidation treatment induced transformation of Cu(OH) 2 /CF from smooth nanorods into a coral-like structure, which exposed more active sites of Cu(OH) 2 /Cu 2 S and enhanced the performance of electrocatalytic hydrogen evolution reaction (HER). Compared with Cu(OH) 2 , Cu(OH) 2 /Cu 2 S showed better alkaline HER performance, especially when the vulcanization concentration was 0.1 M, the overpotential of Cu(OH) 2 /Cu 2 S was 174 mV, and the reaction kinetics was 64 mv dec −1 at a current density of 10 mA cm −2 . In this work, the morphology and electronic structure of copper-based metal sulfide electrocatalysts were adjusted by sulfide treatment, which provided a new reference for improving HER performance.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Chemistry-An Asian Journal
·
2024
·
IF 3.5
Xinxin Dai,Zhen Tang,Xinyuan Yan,Shiyi Tao,Shanshan Wang,Yi Liu,Jiafeng Cao,Xiaolong Deng,Xin Bo
The exploring and developing non-precious transition metal-based catalysts for practical water electrolysis with the low cost, high efficiency and easy macroscopic preparation was still a challenge. Herein, Ni 3 S 2 /Ni(OH) 2 heterojunction with different sulfuration time was proposed and hydrothermally synthesized using a simple two-step approach, which served as a bifunctional electrocatalyst for water splitting in alkaline solution at industrial temperature. Among these catalysts, Ni 3 S 2 /Ni(OH) 2 -5h displayed the smallest overpotentials (237 mV@100 mA cm −2 and 360 mV@100 mA cm −2 ) for OER and HER at room temperature, along with low Tafel slopes of 62.0 mV dec −1 and 80.8 mV dec −1 respectively. Furthermore, working at high temperature Ni 3 S 2 /Ni(OH) 2 -5h exhibited even lower overpotential of 82 mV@100 mA cm −2 at 70 °C for OER and 325 mV@100 mA cm −2 at 60 °C for HER. The excellent performance was ascribed to the heterojunction accelerating the charge transfer, hierarchical interconnected structure promoting the mass transfer and the synergistic effect between the components of Ni 3 S 2 and Ni(OH) 2 . This work could provide a promising route for promoting the electrocatalytic performance of Ni-based catalyst with a simple sulfuration method for industrial water splitting, which could expand to other non-noble metal-based materials for enhancing the electrocatalytic activities.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Changming Zhang,Zhihao Liu,Yongjin Zou,Fen Xu,Lixian Sun,Zhicong Hu,Sensen Yu,Cuili Xiang
Spinel-type materials are attracting great attention as potential electrocatalysts for the hydrogen- and oxygen-evolution reactions. However, their inherent catalytic activities are not on par with those of precious-metal catalysts, and their poor stabilities continue to constrain their rapid development. In this study, we fabricated Zr-CuCo 2 S 4 nanowires and Co(OH) 2 nanosheet–nanowires using electrochemical methods, resulting in lattice defects, sulfur vacancies, and more exposed active sites that catalyze the hydrogen- and oxygen-evolution reactions. DFT calculations revealed that sulfur vacancies enhance intrinsic activity, while Zr doping reduces the energy barrier for H*–OH* adsorption, promotes the formation of additional sulfur vacancies, and modulates active-site Cu/Co valence states, which greatly enhances water-dissociation and H*-adsorption kinetics. Consequently, Zr-CuCo 2 S 4 /Co(OH) 2 requires low overpotentials of 88 and 208 mV, respectively, to support highly stable alkaline hydrogen- and oxygen-evolution reactions at 10 mA cm −2 . This study provides a rational strategy for designing low-cost spinels for green and clean energy.
研究领域:
材料科学
·
电化学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）