JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2018
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IF 5.091
Kui Yin,Shanshan Zhang,Yuyang Sun,Hui Huang,Yafei Cheng,Fan Liao,Mingwang Shao
Hydrogen is considered as a promising energy source because of its high combustion heat and clean products. Among the various hydrogen evolution methods, electrocatalysis is the most convenient and effective way. However it is still problematic to find low-cost and high-efficiency electrocatalysts. In this work, Os-Ag-Si and Os-Au-Si ternary electrocatalysts were investigated. It is interesting that Os-Ag-Si exhibits higher hydrogen evolution performance than that of Os-Au-Si, even though Ag is a worse HER catalyst than Au. The optimal ternary catalyst Os-Ag-Si with mass ratio of 15:15:70 (Os:Ag:Si) was synthesized by a facile Si H bond reduction method, which shows superior hydrogen evolution reaction performance with a low Tafel slope of 40 mV·dec −1 . In addition, the current density of Os-Ag-Si-15 catalyst surpasses that of 20% Pt/C when the overpotential is larger than 160 mV. The introduction of Ag in the Os-Ag-Si ternary catalyst could enhance the electrocatalytic activity, reduce the cost of catalysts, and even be better than Os-Au-Si.
研究领域:
材料科学
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能源化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2024
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IF 2.1
Wei Zijie,Jiang Cuifeng,Chen Xinyan,Li Hui,Song Juan
In this study, Os/Ag nanoparticles (Os/Ag NPs) with smaller K m and almost five times V max compared to Os NPs were prepared. This not only reduced the cost of nanozyme, but also improved the catalytic ability. Addition of L-Cysteine or glutathione (GSH) resulted in inhibitory effect on the peroxidase-like activity of Os/Ag nanozyme. Therefore, the L-Cysteine and GSH could be detected by absorption spectra method with limit of detection (LOD) of 0.22 μM and 0.2 μM, respectively. Moreover, a colorimetric sensing system for L-Cysteine and GSH based on portable paper strip and smartphone was fabricated with LOD of 1.72 μM and 1.4 μM, respectively. Furthermore, this method can be used in fetal bovine serum with recovery rates ranging from 89.42% to 109.0%. The sensing system exhibited good selectivity. This proposed colorimetric sensing assay holds great potential application in the detection of biothiols when no sophisticated equipment are available. Graphical abstract
Scheme illustration for the colorimetric detection of L-Cysteine and GSH by use of smartphone
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
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IF 18.5
Pengfei Xie,Xiao Rong,Xuelian Qin,Min Li,Yan Zuo,Bingjie Liu,Sujiao Cao,Jie Yang,Li Qiu
Ultrasound (US) becomes an appealing modality for stimulating or amplifying immune responses during cancer therapy, which is also termed sono-immunotherapy. However, the clinical prospect has not been fully realized due to the scarcity of efficient sonosensitizers. Herein, for the first time a novel Os-doped Au-tri(pyridin-4-yl) amine coordination structure (Os@Au-TPA)-based sonosensitizer is originally designed and synthesized for sono-immunotherapy of breast-metastasized tumors. Impressively, Os@Au-TPA shows much higher US-mediated 1 O 2 -producing activity than Au-TPA as well as the other traditional sonosensitizers, for example, ≈41.6 folds to ce6, 19.5 times to Protoporphyrin IX (PpIX), 12.0 to Indocyanine Green (ICG), and 11.1 to Iron phthalocyanine (Pc(Fe)). The Os@Au-TPA can not only generate abundant ROS upon US irradiation to implement sonodynamic therapy (SDT), stimulating cell apoptosis and further immunogenic cell death, but can also generate O 2 to alleviate hypoxia to promote the polarization of M2 to M1 macrophages to enhance tumor immunogenicity. As a result, when combined with PD-L1 antibody, it remodels the immunosuppressive tumor microenvironment, achieves concurrent sonodynamic-triggered immune activation, and eradicates both the original and distant-metastasized tumors efficiently. This work not only provides a new strategy to construct potent sonosensitizers from pyridine-metal coordination structures but also proves that sonosensitizers with high performance are crucial in boosting cancer sono-immunotherapy.
研究领域:
肿瘤学
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生物医学工程
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免疫学
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产品关联
DOI
高影响因子
Science China-Chemistry
·
2025
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IF 9.7
Qiu Zhuo,Dai Yiqi,Zhang Runzhi,Xiao Xin,Tong Yuqiao,Yao Lihua,Yan Dongpeng
Cost-effective metal-based electrocatalysts are desirable for industrial water and seawater electrolysis, triggering the hydrogen evolution reaction (HER) with high efficiency. Here, we report the loading of ultralow-content Ru-Os dual-atom onto NiCoP nanoneedles significantly enhancing HER in seawater splitting. The localization of 0.16% Ru and 0.58% Os atoms within NiCoP induces changes in the electronic structure and coordination environment of the Ni active sites. This catalyst achieves low overpotentials (29 and 303 mV in alkaline solution, 68 and 331 mV in alkaline seawater) at 10 and 800 mA/cm 2 , respectively, demonstrating remarkable stability by maintaining performance at 500 mA/cm 2 for 24 h in alkaline and simulated seawater. Experimental and theoretical studies indicate that ultralow content Ru and Os dual atoms are immobilized on the surface of NiCoP/NF through Ru-P and Os-P bonds, leading to electron transfer and changes in the coordination environment. These results enhance the activity of Ni active sits on support, demonstrating superior HER performance in alkaline solution and seawater. Ultralow content Ru and Os dual atoms in NiCoP@Ru-Os/NF play a crucial role in reducing the Gibbs free energy of hydrogen adsorption and lowering the d -band position of the catalyst, thereby accelerating the HER kinetic.
研究领域:
电化学
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催化
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材料科学
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能量转换
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纳米技术
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产品关联
DOI
高影响因子
Small Methods
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2025
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IF 9.1
Shengdong Mu,LiLi Ji,Yao Rao,Zihao Feng,Jiatian Zhu,Ling Bai,Yue Liu,Xingxiu Zhao,Changqing Zhao,Zhifeng Zhao
Real-time and high-efficient detection of food additives is of great significance for global public health. As a popular biosensor, colorimetric detection has been widely applied in various fields, including the food industry. However, owing to the complex structure, weak ROS-catalytic activities and unclear catalytic mechanism, recently reported biocatalysts as biosensors still face major challenges in achieving high-efficient and multimodal aspects. Here, the novel design of high-efficient, robust, multimodal, and Os-based active centers on the oxygen vacancies (O v )-containing Cu 2 Cl(OH) 3 substrates (Os@TBCC-O v ) to serve as ROS-catalytic biosensors to colorimetric detection of food additives is reported. Benefiting from the highly active and excellent electronic structure of Os-single-atom sites, the synthesized Os@TBCC-O v biocatalysts exhibit superior POD-mimetic activities, thus facilitating the exceptional and exclusive detecting activities. Moreover, colorimetric studies found that the POD-mimetic biosensor of Os@TBCC-O v can provide high-efficient, wide-range and visual detection abilities of food additives, including H 2 O 2 , L-Cysteine and glucose, which is much higher efficient than that of previously reported biosensors. Meanwhile, the results of practical application further demonstrate the feasibility of Os@TBCC-O v -based biosensors in detection of food additives. It is suggested that this design will offer a novel pathway for high-efficient colorimetric detection of POD-mimetic biocatalysts in food industry and other related fields.
研究领域:
生物传感器
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食品安全
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纳米技术
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酶模拟物
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分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Nan Wang,Chi Zhang,Houxier Li,Jiahui Wu,Dachuan Zhang,Yan Li,Li Yang,Nan Zhang,Xueyong Wang
This study investigated the in vitro fermentation characteristics of different structural types of Canna edulis resistant starch (RS). RS3 was prepared through a double enzyme hydrolysis method, and RS4 (OS-starch and cross-linked starch) was prepared using octenyl succinic anhydride and sodium trimetaphosphate/sodium tripolyphosphate, respectively. The RS3 and RS4 samples were structurally analyzed using scanning electron microscopy, Fourier-transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction analysis. This was followed by in vitro fermentation experiments. The results revealed microstructure differences in the two groups of starch samples. Compared to native starch, RS3 and RS4 exhibited a lower degree of order and endothermic energy, with lower crystallinity (RS3: 29.59 ± 1.11 %; RS4 [OS-starch]: 28.01 ± 1.32 %; RS4 [cross-linked starch]: 30.44 ± 1.73 %) than that in native starch (36.29 ± 0.89 %). The RS content was higher in RS3 (63.40 ± 2.85 %) and RS4 (OS-starch: 71.21 ± 1.28 %; cross-linked starch: 74.33 ± 0.643 %) than in native starch (57.71 ± 2.95 %). RS3 and RS4 exhibited slow fermentation rates, promoting the production of short-chain fatty acids. RS3 and cross-linked starch significantly increased the production of acetate and butyrate. Moreover, RS3 significantly promoted the abundance of Lactobacillus , while OS-starch and cross-linked starch significantly enhanced the abundance of Dorea and Coprococcus , respectively. Hence, the morphological structure and RS content of the samples greatly influenced the fermentation rate. Moreover, the different varieties of RS induced specific gut microbial regulation. Hence, they show potential applications in functional foods for tailored gut microbiota management.
研究领域:
食品科学
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微生物学
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营养学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
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IF 4.8
Guangwei Zhao,Shengshuo Zhang,Yujie Zou,Nan Jia,Liyuan Zheng,Jinhua Dong
Introduction: Testosterone is a steroid hormone, which plays a pivotal role in regulating metabolism and protein synthesis in the body. The detection of testosterone is of paramount importance for diagnostic purposes in clinical settings, as well as for monitoring athletes’ physiological parameters and ensuring the integrity of sports competitions.Methods: Herein, we present a phage display-derived biosensing platform through genetic engineering of the TS77 antibody variable domains. The variable region genes of the heavy and light chains from TS77 antibody were cloned into the pDong1 plasmid and displayed on the phage surfaces through phage display technology. Subsequently, a novel non-competitive open-sandwich ELISA (OS-ELISA) was developed for testosterone detection, leveraging the antigen-induced interaction changes in antibody variable regions.Results: OS-ELISA based on anti L-chain antibody achieved a limit of detection (LOD) of 2.71 nM and a half-maximal effective concentration (EC50) of 0.22 μM for testosterone detection. Furthermore, the enhanced OS-ELISA platform incorporating purified maltose binding protein fused with VL (MBP-VL) and VH phage demonstrated a LOD of 1.07 pM and a wide working range from 1 pM to 10 mM.Discussion: The OS-ELISA developed in this study exhibits high sensitivity and a broad dynamic range for testosterone quantification, showing significant potential for clinical diagnostics and athlete monitoring applications.
研究领域:
生物化学
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免疫学
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临床诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
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IF 5.2
Qionghua Zheng,Liangming Wang,Xiaoyun Guo,Yebin Feng,Jincheng Chen,Yin Zhang,Qi Zhang,Shaobin He,Wei Chen
Synergistic coupling between Cu and Os endowed nanozyme with dual enzyme-like activities. • Cu modulation simultaneously improved efficiency and reduced noble metal consumption. • Peroxidase- and ascorbate oxidase–like activities were integrated into a single nanozyme. • Multimodal biosensing was realized via colorimetric and fluorescence readouts. Dual metal nanozymes with synergistic catalytic effects provide a versatile platform for biosensing applications. In this work, a carboxylated chitosan–supported Cu/Os dual-metal nanozyme (CC-CuOs) was developed. The incorporation of Cu introduced ascorbate oxidase–like activity, enhanced the intrinsic peroxidase-like performance of the Os-based nanozyme, and reduced noble metal consumption, thereby improving overall cost efficiency. Benefiting from these dual enzyme-like activities, CC-CuOs was employed as a unified catalytic platform to construct two complementary biosensing systems. A cascade colorimetric assay for choline detection was developed based on peroxidase-like activity, exhibiting a linear response over 1–400 μM with a detection limit of 0.85 μM. In parallel, a fluorescence-based sensing strategy for alkaline phosphatase was realized by exploiting the ascorbate oxidase–like activity of CC-CuOs, enabling quantitative detection from 10 to 500 U·L −1 with a detection limit of 0.91 U·L −1 . Both sensing platforms exhibited satisfactory accuracy, selectivity, and reproducibility when applied to human serum. Overall, this study highlights the potential of CC-CuOs nanozymes for constructing multimodal biosensing platforms through synergistic dual enzyme-like activities. Download: Download high-res image (66KB) Download: Download full-size image
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DOI
高影响因子
Orthopaedic Surgery
·
2020
·Vol. 12
·期 2
·645-652
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IF 1.718
Zhen-qun Zhao,Wan-lin Liu,Shi-bing Guo,Rui Bai,Jing-long Yan
Objective To study the role of primary cilia formation disorder and osteoblasts autophagy in the pathogenesis of steroid-induced avascular necrosis of the femoral head (SANFH). Methods Osteoblasts were isolated from rabbit bones and treated with 1 μM Methylprednisolone for 0, 12, 24, 48, and 72 h. The Beclin1, MAP1LC3, Atg-5, Atg-12, IFT20 and OFD1 mRNAs and proteins were detected by PCR and Western blotting, and their correlation was statistically analyzed. The lengths of osteoblast cilia were measured under a laser confocal microscope, and the autophagy flux was tracked by transfecting the osteoblasts with GFP-RFP-LC3 lentivirus. Results Methylprednisolone significantly upregulated Beclin1, MAP1LC3, Atg-5, Atg-12 and OFD1 mRNAs and proteins in a time-dependent manner, and decreased that of IFT20 ( P < 0.05). In addition, the autophagy flux in the osteoblasts also increased and the ciliary length decreased in a time-dependent manner after Methylprednisolone treatment. The length of the cilia were 5.46 ± 0.11 um at 0 h, 4.08 ± 0.09 um at 12 h, 3.07 ± 0.07 um at 24 h, 2.31 ± 0.10 um at 48 h, and finally 1.15 ± 0.04 um at 72 h. Methylprednisolone treatment also affects primary cilium numbers in cultures, for 0 to 72 h. The autophagy regulatory genes, Beclin1, MAP1LC3, Atg-5 and Atg-12, were found to be negatively correlated with IFT20, with an average correlation coefficient of −0.81. A negative correlation was also found between OFD1 and IFT20, with an average correlation coefficient of −0.53. Conclusion Methylprednisolone inhibits primary cilia formation and promotes autophagy, which could be the pathological basis of SANFH. The exact regulatory mechanism needs to be further studied in vivo .
研究领域:
药理学
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骨科
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Orthopaedic Surgery
·
2021
·Vol. 13
·期 4
·1398-1407
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IF 2.071
Ming Li,Yan-bo Xiao,Xin-tao Wang,Jin-peng Zhuang,Chang-long Zhou
Objective To explore the possible way of proline-serine–threonine phosphatase-interacting protein 2 (PSTPIP2) influencing diabetes mellitus-osteoarthritis (DM-OA) progression. Methods In vivo , eight-week-old male Sprague Dawley rats were induced with DM-OA by intraperitoneal injection of streptozotocin with high-fat diet feeding and intra-articular injection of monoiodoacetate. PSTPIP2 overexpression was achieved by intra-articular injection of lentivirus vectors. PSTPIP2 expression was verified by real-time polymerase chain reaction and Western blotting. Histological changes were examined by hematoxylin/eosin and safranin-O/fast-green staining. In vitro , rat synovial fibroblasts were induced DM-OA by stimulation of high glucose (HG) and interleukin (IL)-1β. PSTPIP2 overexpression was achieved by lentivirus infection. U0126 was added as an ERK inhibitor. Levels of tumor necrosis factor (TNF)-α, IL-6, and IL-1β were detected using enzyme-linked immunosorbent assay. Expression of matrix metalloproteinase (MMP)-3, MMP-13, aggrecanase-2 (ADAMTS-5), intercellular cell adhesion molecule (ICAM)-1, extracellular regulated protein kinase (ERK) and phospho-ERK (p-ERK) was detected by Western blotting. Results In DM-OA rats, PSTPIP2 relative messenger RNA (mRNA) level was significantly decreased compared to control rats. The protein expression was also decreased obviously. Inflammation score in synovium was dramatically increased, accompanying with increased TNF-α, IL-6, and IL-1β levels. Osteoarthritis research society international (OARSI) score in cartilage was markedly increased, along with increased MMP-3, MMP-13, ADAMTS-5, ICAM-1, ERK and p-ERK expression. In PSTPIP2-overexpressed DM-OA rats, PSTPIP2 mRNA level and protein expression was increased compared to DM-OA rats received negative-control lentivirus vectors. The inflammation score, as well as TNF-α, IL-6, and IL-1β levels were dramatically decreased. Also, the OARSI score and protein expression of MMP-3, MMP-13, ADAMTS-5, ICAM-1, ERK and p-ERK were decreased. In HG+IL-1β-treated rat synovial fibroblasts, PSTPIP2 protein expression was decreased compared to normal glucose (NG)-treated cells. Levels of TNF-α, IL-6, and IL-1β, as well as expression of MMP-3, MMP-13, ADAMTS-5, ICAM-1, ERK and p-ERK were increased. After cells were infected with PSTPIP2-overexpressed lentivirus, levels of TNF-α, IL-6, and IL-1β, and expression of MMP-3, MMP-13, ADAMTS-5, ICAM-1, ERK and p-ERK were obviously decreased compared to cells infected with NC lentivirus. In addition, ERK inhibitor U0126 treatment also decreased the TNF-α, IL-6, and IL-1βlevels and MMP-3, MMP-13, ADAMTS-5, ICAM-1, ERK and p-ERK expression in HG + IL-1β treated rat synovial fibroblasts. Conclusion Overexpression of PSTPIP2 alleviates synovial inflammation and cartilage injury during DM-OA progression via inhibiting ERK phosphorylation.
研究领域:
医学研究
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风湿病学
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糖尿病学
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DOI
PubMed
高影响因子
Orthopaedic Surgery
·
2015
·Vol. 7
·期 2
·161-167
·
IF 0
Hui Yu,Wen-bin Ye,Zhao-ming Zhong,Ruo-ting Ding,Jian-ting Chen
Objective Advanced oxidation protein products (AOPPs), a marker of oxidative stress, are prevalent in many kinds of disorders. Osteoarthritis (OA), mainly resulting from the regression of cartilage, chronic inflammation of the synovium and the subchondral bone remodeling. Although the inflammatory response of AOPPs on fibroblast-like synoviocytes (FLSs) were reported, the effect of AOPPs on cartilage and synovial in vivo remains unclear. Therefore, our study aims to investigate whether AOPPs have an effect on the articular cartilage and synovial in a rabbit model of OA. Methods OA model were created by anterior cruciate ligament transection and medial meniscus resection (ACLT + MMx). Forty-eight male New Zealand rabbits were randomly divided into 3 groups: sham-operated group, AOPPs/ACLT + MMx group, and phosphate buffered saline (PBS)/ACLT + MMx group. In sham-operated group, the anterior cruciate ligament was just exposed without transection, and then the incision was sutured. Then intra-articular injection of AOPPs or PBS was performed in the other two groups. Through four weeks and eight weeks of treatment, rabbits in each group were sacrificed. Both hind legs were removed. India ink staining and Safranin O and fast green staining were used to evaluate the macroscopic and microscopic cartilage morphology. The protein expression of matrix metalloproteinases (MMP)-3, MMP-13 in synovium was measured by Western blot. Result The India ink score and Mankin score of AOPPs/ACLT + MMx group were both higher than the other two groups at the two time points. Western blot have revealed that intra-articular injection of AOPPs upregulated the protein expression of MMP-3 and MMP-13 in synovium. Conclusion AOPPs participated in the occurrence and development of OA by upregulating the protein expression of MMP-3 and MMP-13 in synovium.
研究领域:
骨科
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病理学
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风湿病学
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生物化学
✓ 已验证
DOI
PubMed
Orthopaedic Surgery
·
2020
·Vol. 12
·期 6
·1947-1953
·
IF 1.718
Yuan-jun Xia,Wei Wang,Hong Xia,Xian-hua Huang,Feng-piao Deng,Qing-shui Ying,Xiang Yu,Li-hua Li,Jian-hua Wang,Ying Zhang
Objective Spinal fusion is one of the most common surgical interventions for spine reconstruction. Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed. The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion. In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity. The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion. However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres. This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion. Methods The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats. All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group. The microsphere morphology was scanned and analyzed using a scanning electron microscope. Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery. Hematoxylin and eosin staining was conducted for histological analysis. Both alkaline phosphatase (ALP) and calcium content were measured. Results The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared. Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres. No side effects, infections, or abnormal behaviors were found in the animals. After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group. No ectopic osteogenesis was found in the vertebral canal or other muscle tissues. After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen. Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups. Conclusion The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content. These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.
研究领域:
骨科
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生物医学工程
·
组织工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）