SCIENCE OF THE TOTAL ENVIRONMENT
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2022
·
IF 10.753
Jian-Jie Gao,Bo Wang,Zhen-Jun Li,Jing Xu,Xiao-Yan Fu,Hong-Juan Han,Li-Juan Wang,Wen-Hui Zhang,Yong-Dong Deng,Yu Wang,Ze-Hao Gong,Yong-Sheng Tian,Ri-He Peng,Quan-Hong Yao
Industrial thiocyanate (SCN − ) waste streams from gold mining and coal coking have caused serious environmental pollution worldwide. Phytoremediation is an efficient technology in treating hazardous wastes from the environment. However, the phytoremediation efficiency of thiocyanate is very low due to the fact that plants lack thiocyanate degradation enzymes. In this study, the thiocyanate hydrolase module was assembled correctly in rice seedlings and showed thiocyanate hydrolase activity. Rice seedlings engineered to express thiocyanate degrading activity were able to completely remove thiocyanate from coking wastewater. Our findings suggest that transforming the thiocyanate hydrolase module into plants is an efficient strategy for rapid phytoremediation of thiocyanate in the environment. Moreover, the rice seedlings expressing apoplastic or cytoplasmic targeted thiocyanate hydrolase module were constructed to compare the phytoremediation efficiency of secretory/intracellular recombinant thiocyanate hydrolase. The most obvious finding from this study is that the apoplastic expression system is more efficient than the cytoplasm expression system in the phytoremediation of thiocyanate. At last, this research also shows that the secreted thiocyanate hydrolase from engineered rice plants does not influence rhizosphere bacterial community composition.
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Food and Bioprocess Technology
·
2024
·
IF 5.3
Hu Jin,Yang Wencheng,Lan Huanling,Lin Xueyuan,Li Chang
Rice ( Oryza sativa ) bran is a by-product of rice processing, which contains rich nutrients and is a resource that can be fully utilized. However, lipase in rice bran can lead to rancidity of rice bran oil. Therefore, it is important to study the characteristics of rice bran lipase for the stable storage of rice bran. A new lipase from rice bran was isolated by DEAE Sepharose column and gel filtration column, respectively. The determination of lipase’s molecular weight and purity was conducted utilizing the SDS-PAGE technique, and the enzyme was identified through LC-MS/MS. Concurrently, an investigation was conducted on the characteristics of the lipase. The findings indicated that the lipase had a molecular weight of 36.352 kDa. Optimal catalytic activity for rice bran lipase (RBL) was observed at a temperature of 40 ℃ and pH value of 7.0, while it exhibited remarkable stability below 20 ℃ and within the pH range from 6.0 to 8.0. The stability of the RBL against various metal ions and high NaCl concentrations was observed to be relatively modest. Notably, the catalytic activity was significantly influenced by certain organic solvents (such as acetone, THF, and trichloromethane) as well as several inhibitors ( P < 0.05). Furthermore, RBL demonstrated a preference for hydrolyzing long-chain p-NP esters (C12-C16).
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RSC Advances
·
2026
·Vol. 16
·期 17
·15187
·
IF 6.1
Jiajia Yang,Ruihua Chen,Huan Zhang,Ruonan Wang,Zhifeng Zhang
Elucidating the phytotoxicity of silver nanoparticles (AgNPs) at the primary tissue level is essential for a comprehensive assessment of their ecotoxicological risks. This study systematically investigated the phytotoxic mechanisms of polyethyleneimine-coated silver nanoparticles (AgNPs@PEI) on plant primary roots using rice ( Oryza sativa L.) primary roots as an experimental model through integrated physiological, transcriptomic, and metabolomic analyses. Exposure to AgNPs@PEI induced concentration-dependent growth inhibition, with a 10 mg L −1 treatment causing a 44% reduction in root length, a 50% decrease in biomass, and a 28.3% shortening of the root meristematic zone, accompanied by abnormal root hair development and cap detachment. Physiological assessments revealed significant disturbances in root vitality and antioxidant enzyme activities (SOD, POD, and CAT). Transcriptomic profiling identified significant downregulation (fold change > 2) of genes involved in nine key metabolic pathways, most notably phenylpropanoid biosynthesis, aromatic amino acid (phenylalanine/tyrosine/tryptophan) biosynthesis, and glutathione metabolism. Metabolomic analysis revealed concomitant disruptions in seven essential pathways, particularly those involved in unsaturated fatty acid biosynthesis. These transcriptomic and metabolic alterations collectively impaired cell wall lignification through suppression of the phenylpropanoid pathway and compromised membrane integrity via dysregulation of fatty acid metabolism. This research elucidates the phytotoxic effects of AgNPs from a primary plant tissue perspective, providing novel evidence for understanding nanomaterial–plant interactions at the tissue level. Elucidating the phytotoxicity of silver nanoparticles (AgNPs) at the primary tissue level is essential for a comprehensive assessment of their ecotoxicological risks.
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ENVIRONMENTAL POLLUTION
·
2017
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IF 5.099
Biying Wu,Lizhong Zhu,X. Chris Le
The wide occurrence and high environmental concentration of titanium dioxide nanoparticles (nano-TiO 2 ) have raised concerns about their potential toxic effects on crops. In this study, we employed a GC-MS-based metabolomic approach to investigate the potential toxicity of nano-TiO 2 on hydroponically-cultured rice ( Oryza sativa L.) after exposed to 0, 100, 250 or 500 mg/L of nano-TiO 2 for fourteen days. Results showed that the biomass of rice was significantly decreased and the antioxidant defense system was significantly disturbed after exposure to nano-TiO 2 . One hundred and five identified metabolites showed significant difference compared to the control, among which the concentrations of glucose-6-phosphate, glucose-1-phosphate, succinic and isocitric acid were increased most, while the concentrations of sucrose, isomaltulose, and glyoxylic acid were decreased most. Basic energy-generating ways including tricarboxylic acid cycle and the pentose phosphate pathway, were elevated significantly while the carbohydrate synthesis metabolism including starch and sucrose metabolism, and glyoxylate and dicarboxylate metabolism were inhibited. However, the biosynthetic formation of most of the identified fatty acids, amino acids and secondary metabolites which correlated to crop quality, were increased. The results suggest that the metabolism of rice plants is distinctly disturbed after exposure to nano-TiO 2 , and nano-TiO 2 would have a mixed effect on the yield and quality of rice.
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ENVIRONMENTAL AND EXPERIMENTAL BOTANY
·
2022
·
IF 6.028
Wenxiao Zhang,Haiou Wang,Qian Bai,Xintong Li
To understand uptake and accumulation process of organophosphate esters (OPEs), growth, concentrations of OPEs, and transcript regulation of lipid transporters in rice seedlings were investigated under six OPE congeners exposures. The increase of seedlings’ growth and accumulation of OPEs in plants were observed. Root concentration factors (RCFs) of aryl-OPEs were the highest in consistent with their strong hydrophobicity, indicating that they were more liable to be accumulated in roots. Meanwhile, shoot/root concentration factors (SRCFs) of halogenated alkyl-OPEs were the highest in accordance with their low hydrophobicity, implying that they were easily translocated to shoots. Lipid transporters involved in uptake and accumulation of OPEs, evidence from the transcript responses of their lipid transporters. Moreover, the only positive relationship between OsTIL expressions and accumulation of OPEs demonstrated the dominant role of OsTIL among the investigated transporters. In addition, although all of OPE congeners could be docked into active cavities of OsTIL with varied markedly binding affinities, hydrophobicity OPEs performed stronger binding energy with OsTIL after homology modeling and molecular docking analysis. Therefore, uptake and accumulation of OPEs were corporately charged by OPEs’ physicochemical properties and lipid carrier transports in coincidence with the experimental observation about accumulation of OPEs in roots and shoots.
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PLoS One
·
2018
·Vol. 13
·期 4
·e0195754
·
IF 2.766
Yongsheng Zhu,Hanju Sun,Shudong He,Qiuyan Lou,Min Yu,Mingming Tang,Lijun Tu
Anthocyanins are naturally active substances. In this study, anthocyanins from black rice were obtained by membrane filtration and column chromatography separation. Five anthocyanin monomers in black rice extract were identified by HPLC-MS/MS, and the major anthocyanin monomer (cyanidin-3-glucoside, C3G) was purified by preparative HPLC (Pre-HPLC). The proliferative effects of the anthocyanins on Bifidobacteria and Lactobacillus were investigated by determining the media pH, bacterial populations and metabolic products. After anaerobic incubation at 37 °C for 48 h, not only the pH of the media containing C3G was lower than that of the extract of black rice anthocyanin (BRAE), but the numbers of both Bifidobacteria and Lactobacillus were also significantly increased. Furthermore, hydroxyphenylpropionic, hydroxyphenylacetic, and hydroxybenzoic acids and other metabolites were detected by GC-MS in vitro . Our results revealed that the anthocyanins and anthocyanin monomers from black rice had prebiotic activity and they were metabolized into several small molecules by Bifidobacteria and Lactobacillus.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yimei Wang,Shuai Huang,Zongrun Li,Jundan Tong,Yile Sheng,Chun Ye,Weixu Huo,Yafan Zhao,Yanxiu Du,Ting Peng,Jing Zhang
Arsenic (As) contamination severely limits plant growth and poses a significant threat to human health due to its accumulation in crops like rice. Phytochrome B (PhyB) regulates plant development and responses to abiotic stress, but its role in modulating As stress and regulating As accumulation in rice remains unclear. In this study, we examined the physiological and molecular responses of rice phytochrome B ( OsPHYB ) knockout ( OsPHYB -KO) lines compared with wild-type (WT) plants under arsenite [As(III)] stress. Under As(III) exposure for 7 days, OsPHYB -KO seedlings exhibited significantly increased root length (40.42 %-58.08 %), shoot height (7.41 %-9.22 %), and dry biomass (18.88 %-37.41 %) compared with WT. Chlorophyll fluorescence analysis showed enhanced photosynthetic efficiency in OsPHYB -KO lines, with significant increases in maximum photochemical efficiency of PSII (Fv/Fm) and effective photochemical quantum yield of PSII (φPSII). Notably, As concentrations in both shoots and roots of OsPHYB -KO plants were reduced by 39.78 %-51.89 % and 28.26 %-33.79 %, respectively, compared with WT. OsPHYB -KO lines had 25.56 %-55.63 % higher antioxidant enzyme activities and 43.10 %-54.60 % lower malondialdehyde levels, indicating improved tolerance to oxidative stress. Transcriptomic profiling and qRT-PCR revealed upregulation of genes involved in melatonin (MT) biosynthesis and glutathione (GSH) metabolism, with MT content increased by 16.20 %-67.96 % and GSH content elevated by 38.51 %-117.12 % in both roots and shoots of OsPHYB -KO plants. These results demonstrate that OsPHYB plays a crucial role in As(III) detoxification and provide a foundation for developing low-As rice cultivars.
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PLANT GROWTH REGULATION
·
2025
·
IF 3.9
Tu Chun Yan,Gao Yong Qiang,Zheng Lu,Ma Jun Wei,Shen Ren Fang,Zhu Xiao Fang
Aluminum (Al) toxicity represents a significant constraint on crop growth in acidic soils. Al stress can stimulate the biosynthesis of melatonin (MT) in rice ( Oryza sativa L.). The exogenous application of 20 μM MT markedly reduces Al accumulation in root tips and effectively mitigates Al stress. By enhancing the levels of root hemicellulose and down-regulating the expression of two genes sensitive to aluminum rhizotoxicity ( OsSTAR1 and OsSTAR2) , which function to obscure Al binding sites within the cell wall, MT significantly improves the fixation of Al by the cell wall. Furthermore, MT diminishes cytoplasmic entry of Al by reducing expression levels of nramp aluminum transporter 1 gene ( OsNRAT1 ), while inducing expression of an aluminum-sensitive gene ( OsALS1 ), thereby promoting vacuolar compartmentation to lower cytoplasmic Al concentration. Additionally, MT substantially decreases Nitric oxide (NO) accumulation induced by Al in roots. The alleviation effect exerted by MT on Al toxicity can be replicated through administration of the NO scavenger carboxyl-PTIO (c-PTIO). Moreover, the Al-sensitive phenotype exhibited in the osgsnor mutant, coupled with the absence of mitigation by MT, further substantiates that MT alleviates Al toxicity in rice primarily via regulation of NO content, potentially relying on NO signal transduction pathways. In summary, MT plays a pivotal role in modulating rice resistance to Al toxicity and stimulates multiple pathways to enhance tolerance against this abiotic stressor. This study provides new insights into understanding mechanisms underlying rice resistance to abiotic stresses.
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Agriculture-Basel
·
2021
·Vol. 11
·期 12
·1247
·
IF 2.925
Haipeng Zhang,Rui Wang,Zhiqing Chen,Peiyuan Cui,Hao Lu,Yanju Yang,Hongcheng Zhang
Zinc oxide nanoparticles (ZnO NPs) have been applied widely in agriculture, and many studies were conducted to evaluate the effect of ZnO NPs on plant growth. So far, few studies have been investigated with regard to the potential effect of ZnO NPs on cereal yield formation or Zn content in grains. Herein, we used a pot experiment, which was conducted involving five dosages of Zn (0.3, 0.6, 1.2, 2.4, and 4.8 g Zn pot−1), to evaluate the impacts which ZnO NPs made in rice yield, dry matter accumulation, rice quality and grain Zn contents. The results demonstrated that ZnO NPs increased the production of grain, dry matter accumulation and particulate Zn content. Compared with control treatment, ZnO NPs application presented higher rice yield with more panicle number (4.83–13.14%), spikelets per panicle (4.81–10.69%), 1000-grain weight (3.82–6.62%) and filled grain rate (0.28–2.36%). Additionally, the dry jointing, heading and mature periods, LAI, SPAD values, and photosynthetic potentials of ZnO NPs were all significantly higher relative to treatment without ZnO NPs. The more photosynthetic substances and higher dry matter accumulated in the whole rice growing stage resulted in higher rice grain yield. Furthermore, ZnO NPs increased brown rice rate, milled rice rate, head rice rate, chalkiness size, chalkiness grain rate, chalkiness degree, amylose content and protein content, improving rice processing and appearance qualities. For the Zn nutrition in rice grain, ZnO NPs application significantly increased the Zn content of edible polished rice and promoted the relocation of Zn from the aleurone layer. This study effectively demonstrated that ZnO NPs could be a potential high-performed fertiliser for enhancing rice yield and quality.Keywords:Zinc oxide nanoparticles;yield formation;rice quality;photosynthetic potential
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Journal of Advanced Research
·
2020
·
IF 6.992
Yuanze Zhou,Lijing Liao,Xikai Liu,Biao Liu,Xinxin Chen,Yan Guo,Chuanlong Huang,Yucheng Zhao,Zhixiong Zeng
Plant tryptophan decarboxylase (TDC) is a type II Pyridoxal-5′-phosphate-dependent decarboxylase (PLP_DC) that could be used as a target to genetically improve crops. However, lack of accurate structural information on plant TDC hampers the understanding of its decarboxylation mechanisms. In the present study, the crystal structures of Oryza sativa TDC ( O sTDC) in its complexes with pyridoxal-5′-phosphate, tryptamine and serotonin were determined. The structures provide detailed interaction information between TDC and its substrates. The Y359 residue from the loop gate is a proton donor and forms a Lewis acid-base pair with serotonin/tryptamine, which is associated with product release. The H214 residue is responsible for PLP binding and proton transfer, and its proper interaction with Y359 is essential for Os TDC enzyme activity. The extra hydrogen bonds formed between the 5-hydroxyl group of serotonin and the backbone carboxyl groups of F104 and P105 explain the discrepancy between the catalytic activity of TDC in tryptophan and in 5-hydroxytryptophan. In addition, an evolutionary analysis revealed that type II PLP_DC originated from glutamic acid decarboxylase, potentially as an adaptive evolution of mechanism in organisms in extreme environments. This study is, to our knowledge, the first to present a detailed analysis of the crystal structure of Os TDC in these complexes. The information regarding the catalytic mechanism described here could facilitate the development of protocols to regulate melatonin levels and thereby contribute to crop improvement efforts to improve food security worldwide.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Shangguang Du,Jun Luo,Xutang Tu,Zuozuo Ai,Dong Wu,Zhengrong Zou,Liping Luo
Low temperatures significantly impact on rice ( Oryza sativa ) yield and quality. Traditional metabolomic techniques, often involving time-consuming chromatography-mass spectrometry procedures, are currently in use. This study investigated metabolomic responses of rice seedlings under low-temperature stress using nanoliter electrospray ionization mass spectrometry (nanoESI-MS) in combination with multivariate analysis . Results revealed distinct metabolic profiles in ‘Qiutianxiaoting’ ( japonica ) and ‘93-11′ ( indica ) rice seedlings. Among the 36 identified compounds in rice, seven key metabolites, comprising l -glutamic acid, asparagine, tryptophan, citric acid, α-linolenic acid, malic acid, and inositol, were identified as responsive to cold stress. Notably, malic acid content reached 1332.40 μg/g dry weight in Qiutianxiaoting and 1444.13 μg/g in 93-11. Both the qualitative and quantitative results of nanoESI-MS were further confirmed through gas chromatography-mass spectrometry validation. The findings highlight the potential of nanoESI-MS for rapidly characterizing crucial metabolites across diverse plant species under exposure to stress.
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Frontiers of Environmental Science & Engineering
·
2020
·Vol. 14
·期 6
·1-12
·
IF 4.053
Zhang Yingdan,Liu Na,Wang Wei,Sun Jianteng,Zhu Lizhong
Titanium dioxide nanoparticle (nano-TiO 2 ), as an excellent UV absorbent and photo-catalyst, has been widely applied in modern industry, thus inevitably discharged into environment. We proposed that nano-TiO 2 in soil can promote crop yield through photosynthetic and metabolic disturbance, therefore, we investigated the effects of nano-TiO 2 exposure on related physiologic-biochemical properties of rice ( Oryza sativa L.). Results showed that rice biomass was increased >30% at every applied dosage (0.1–100 mg/L) of nano-TiO 2 . The actual photosynthetic rate (Y(II)) significantly increased by 10.0% and 17.2% in the treatments of 10 and 100 mg/L respectively, indicating an increased energy production from photosynthesis. Besides, non-photochemical quenching (Y(NPQ)) significantly decreased by 19.8%–26.0% of the control in all treatments respectively, representing a decline in heat dissipation. Detailed metabolism fingerprinting further revealed that a fortified transformation of monosaccharides (D-fructose, D-galactose, and D-talose) to disaccharides (D-cellobiose, and D-lactose) was accompanied with a weakened citric acid cycle, confirming the decrease of energy consumption in metabolism. All these results elucidated that nano-TiO 2 promoted rice growth through the upregulation of energy storage in photosynthesis and the downregulation of energy consumption in metabolism. This study provides a mechanistic understanding of the stress-response hormesis of rice after exposure to nano-TiO 2 , and provides worthy information on the potential application and risk of nanomaterials in agricultural production.
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