MATERIALS LETTERS
·
2016
·
IF 2.437
Xinyu Peng,Yixin Zhang,Ying Chen,Sai Li,Bin He
A well-defined miktoarm star copolymer (μ-PEG-PCL-PLLA) was synthesized and the crystallization behavior of the copolymers was investigated. The structure of copolymer was confirmed by 1 H NMR, the molecular weight and molecular weight distribution were studied by GPC . XRD and POM were used to study the crystallization behavior of the miktoarm star copolymers. The XRD spectra showed that the miktoarm star copolymer showed low crystallinity for the joint of terminal, which limited the mobility of polymeric chains. The POM photographs revealed that PCL segments formed spherulites with PLLA crystalline grains as nucleus due to the good compatibility between PCL and PLLA.
研究领域:
材料科学
·
高分子化学
·
生物医学材料
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DOI
高影响因子
Materials Research Express
·
2021
·Vol. 8
·期 5
·055402
·
IF 1.62
Lu Pang,Peixin Sun,Xufeng Dong,Tao Tang,Yi Chen,Qiang Liu,Min Qi
Articular cartilage has limited self-repair capacity due to the lack of vascularization, innervation and lymphatic networks. Biomimetic scaffolds with features of the extracellular matrix (ECM) of cartilage are advantageous to repair the injured cartilage tissue, but it remains a challenge to regulate its shear viscoelasticity to meet the needs of applications as articular cartilages. Fiber reinforced hydrogel is of great significance for their clinical application as cartilage tissue engineering scaffolds, especially for repairing the fibrocartilage tissue like meniscus or temporomandibular joint disc. In order to promote the shear viscoelasticity of alginate hydrogels, which was seldom studied, electrospinning PCL nanofiber layers were added into the alginate hydrogels to prepare PCL nanofibers reinforced alginate hydrogel composites (PNRAHCs). Compared with neat alginate hydrogel scaffolds, the PNRAHCs presented coral-like structure and spider web-like structure, and some PCL nanofibers form reinforced fiber bundles. Those special structures make the PNRAHCs have higher porosity, higher shear storage modulus and higher shear loss modulus than the neat alginate hydrogels, indicating better shear mechanical properties. They have the potential to be applied as the scaffolds to repair fibrocartilage tissues.
研究领域:
生物材料学
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组织工程学
·
再生医学
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DOI
高影响因子
PLoS One
·
2014
·Vol. 9
·期 11
·e112200
·
IF 3.534
Jian Jin,Bowen Sui,Jingxin Gou,Jingshuo Liu,Xing Tang,Hui Xu,Yu Zhang,Xiangqun Jin
In this content, a small molecular ligand of prostate specific membrane antigen (SMLP) conjugated poly (caprolactone) (PCL)-b-poly (ethylene glycol) (PEG) copolymers with different block lengths were synthesized to construct a satisfactory drug delivery system. Four different docetaxel-loaded polymeric micelles (DTX-PMs) were prepared by dialysis with particle sizes less than 60 nm as characterized by dynamic light scattering (DLS) and transmission electron microscope (TEM). Optimization of the prepared micelles was conducted based on short-term stability and drug-loading content. The results showed that optimized systems were able to remain stable over 7 days. Compared with Taxotere, DTX-PMs with the same ratio of hydrophilic/hydrophobic chain length displayed similar sustained release behaviors. The cytotoxicity of the optimized targeted DTX-PCL 12K -PEG 5K -SMLP micelles (DTX-PMs2) and non-targeted DTX-PCL 12K -mPEG 5K micelles (DTX-PMs1) were evaluated by MTT assays using prostate specific membrane antigen (PSMA) positive prostate adenocarcinoma cells (LNCaP). The results showed that the targeted micelles had a much lower IC50 than their non-targeted counterparts (48 h: 0.87±0.27 vs 13.48±1.03 µg/ml; 72 h: 0.02±0.008 vs 1.35±0.54 µg/ml). In vitro cellular uptake of PMs2 showed 5-fold higher fluorescence intensity than that of PMs1 after 4 h incubation. According to these results, the novel nano-sized drug delivery system based on DTX-PCL-PEG-SMLP offers great promise for the treatment of prostatic cancer.
研究领域:
药学
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癌症研究
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纳米技术
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
Discover Applied Sciences
·
2024
·Vol. 6
·期 4
·1-16
·
IF 0
Liu Xiaohong,Basta Altaf H.,Liu Rui,Fu Shiyu
This study describes the preparation of cellulose nanofibers (CNFs) with varying amounts of carboxyl groups from rice straw pulp using the TEMPO/NaBr/NaClO oxidation system. The resulting CNFs were found to be in the form of nanofibers with an average diameter of 6 nm and an average length of 160 nm. To further enhance their properties, the CNFs were grafted with polycaprolactone (PCL) to create CNFs-g-PCL, which was then blended with shape memory polyurethane (SMPU) to produce CNFs-g-PCL/SMPU composites. It was observed that as the carboxyl content in CNFs increased from 0.35 to 1.14 mmol/g, the graft ratio of PCL on CNFs decreased from 24.6 to 10.7%. Consequently, the hydrophobicity of the grafted product (CNFs-g-PCL) also decreased. When 10% CNFs-g-PCL was added to the SMPU matrix, the elastic modulus and tensile stress of the resulting composite were both higher than those of the pure SMPU, increasing by up to 54.4% and 67.3%, respectively. Additionally, the shape retention and shape recovery rates of the composite remained stable after addition of CNFs-g-PCL. In conclusion, incorporating CNFs-g-PCL into SMPU can improve its mechanical properties while maintaining its shape memory properties. Graphical abstract
研究领域:
材料科学
·
高分子化学
·
纳米技术
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DOI
POLYMER ENGINEERING AND SCIENCE
·
2014
·Vol. 55
·期 1
·223-230
·
IF 1.441
Zhao-Xia Li,Man-Geng Lu,Kun Wu,Yun-Fei Zhang,Lei Miao,Yin-Wen Li,Hui-Long Guo,Jian Zheng
A series of novel thermosensitive macroporous poly (ethylene glycol) (PEG)- co -poly( N -isopropylacrylamide) (PNIPAAm)- co -poly (ε-caprolactone) (PCL) hydrogels were synthesized via in situ free radical polymerization. Poly(ethylene glycol diacrylate) (PEGDAc) and poly(ε-caprolactone diacrylate) (PCLDAc) were prepared as macrocrosslinkers. All compounds were investigated by Nuclear Magnetic Resonance (NMR) and Fourier transform-infrared spectroscopy (FT-IR). Differential Scanning Calorimetry (DSC) results showed the lower critical solution temperatures (LCSTs) of the gels were at around 31°C. The macroporous gels not only had considerable swelling ratios, but also exhibited rapid swelling kinetics and response sensitivity. Above mentioned hydrogels showed a remarkable oscillatory swelling–deswelling transition, making them have potential application in long-term drug delivery. POLYM. ENG. SCI., 55:223–230, 2015. © 2014 Society of Plastics Engineers
研究领域:
材料科学
·
生物医学工程
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DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Jianhang Du,Xiaogang Bao,Jing Wen,Chen Tang,Chenxu Wang,Changgui Shi,Chengqing Yi,Guohua Xu,Dejian Li
Bone defects caused by osteoporosis present a significant clinical challenge. The primary obstacles to osteoporotic bone regeneration are the persistent decline in osteogenic differentiation potential of bone marrow mesenchymal stem cells (BMSCs) and the abnormally high activity of osteoclasts. Emerging research highlights the crucial role of immunomodulation in osteoporosis. Harnessing the immunomodulatory capabilities of bioactive materials to improve the compromised osteoporotic microenvironment may enhance the osteogenic differentiation potential of BMSCs while reducing osteoclast differentiation and resorption. This strategy offers promising avenues for treating osteoporotic bone defects. In this study, we developed an innovative 3D-printed SrCO 3 @PCL/PDA composite scaffold. Through 3D printing, the polycaprolactone (PCL) matrix was customized to achieve biomimetic structural and mechanical design. The introduced strontium carbonate (SrCO 3 ) allows for the responsive release of Sr 2+ ions in the acidic osteoporotic microenvironment, suppressing osteoclast activity and maintaining a regenerative-friendly environment. The polydopamine (PDA) coating enhances the biocompatibility of the scaffold, thereby promoting cell adhesion and proliferation on its surface. Notably, this novel composite scaffold effectively promotes macrophage polarization towards the M2 phenotype rather than the M1 phenotype, exerting an immunomodulatory effect that improves osteoporotic bone regeneration. In vivo experiments further validated our hypothesis. This innovative composite scaffold offers a promising strategy for the comprehensive treatment of osteoporotic bone defects.
研究领域:
生物材料学
·
骨科医学
·
免疫学
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产品关联
DOI
高影响因子
Surfaces and Interfaces
·
2021
·
IF 4.837
Ying Wang,Yuezhou Liu,Yongfang Qian,Lihua Lv,Xiyue Li,Yanjing Liu
In this work, the antibacterial nanofibers of MgO/PCL/PVP (MCV) used for tissue engineering materials were prepared using PCL/PVP (CV) as nanofiber substrates and magnesium oxide nanoparticles (MgONPs) as antibacterial agents by electrospinning technology. The suitable CV substrates with excellent microscopic morphology and small average diameter of 208.5 ± 74.9 nm were prepared by electrospinning the mixture of PCL and PVP with the mass ratio of 1: 1. The MCV antibacterial nanofibers were successfully fabricated by loading MgONPs onto the suitable CV substrates by electrospinning process. In addition, the characterizations including scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) indicated that 3.0 wt% MgONPs were physically and evenly loaded onto the CV substrates (MCV-3.0), which presented an excellent antibacterial rate of 100% against Escherichia coli ( E. coli ).
研究领域:
生物材料学
·
组织工程学
·
医学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2022
·Vol. 139
·期 29
·e52649
·
IF 3.057
Chen Gao,Junbo Jiang,Juan Wang,Yutong Wang,Yangyang Zhang,Caihua Rao,Yue Cheng,Zhongrong Chen,Runhuai Yang,Gang Zhao
Mineralization of hydroxyapatite mineralized by acidic metal oxides has attracted substantial research interest in the research and development of orthopedic substitutes. In this study, Polycaprolactone (PCL) nanofibers with nano-tantalum oxide (Ta 2 O 5 ) were successfully fabricated through electrospinning. The scaffold simulates the mineralization process of hydroxyapatite in simulated body fluid. However, in the process of mineralization, there is a chemical reaction between hydrophobic scaffolds and acid oxides, which is believed to have a significant impact on the biological effects of the scaffolds. The results of SEM, EDS, XRD, Mapping, FTIR, GPC, 13 CNMR, and 1 HNMR explain the principle of hydroxyapatite mineralization by Ta 2 O 5 and the reason why the composite scaffold is blurred, that is, the carbonyl carbon of PCL attacked by the surface hydroxyl groups of Ta 2 O 5 to catalyze its ring-opening polymerization to synthesize PCL/Ta 2 O 5 nanocomposites in one step. In order to accurately explore the effect of this reaction on the metabolism of biological macromolecules in the future, we provide a detailed route of Ag Nanoparticles -Dimercaptosuccinic acid@Transcription activator (AgNPs-DMSA@TAT) probe synthesis. The results provide a reference for the sedimentation of hydroxyapatite induced by acid oxides and its effect on traditional electrospun fiber scaffolds.
研究领域:
材料科学
·
生物材料
·
骨科
·
生物医学工程
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DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 3.616
Meifeng Liu,Weichi Chen,Xingyu Zhang,Pengwen Su,Feng Yue,Shaoqun Zeng,Song Du
Madecassoside (MA) exhibits excellent therapeutic effects in wound healing and scar management. However, its high hydrophilic nature and low permeability through skin tissue limits its topical application. Liposomes are widely used to deliver drugs due to their high structural similarity and biocompatibility with cell membranes. However, normal liposome formulations are too fluid to maintain sufficient adhesion to the wound surface. In this study, in order to make an MA formulation conducive to topical administration, poly(ethylene glycol)-poly( ε -caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE), a biodegradable and temperature-responsive copolymer material, was synthesized and applied to improve the adhesion properties of MA liposomes. The mean particle size of the PECE-modified MA liposomes was 213.43±4.68 nm, and the zeta potential was −23.80±15.37 mV under the optimal conditions of EPC (egg yolk lecithin) to PECE at a mass ratio of 1:1. Additionally, PECE-modified MA liposomes maintained a hydrogel state for better adhesion until the temperature reached 43°C. Furthermore, the PECE-modified MA liposomes showed superior wound contraction effects relative to the MA liposomes in second-degree burn experiments using a rat model. These results indicated that PECE-modified MA liposomes have better surface adhesion performance and healing effects than unmodified MA liposomes.
研究领域:
药剂学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Huiyuan Zhang,Kaiming Wang,Pei Zhang,Wenxiu He,Aixin Song,Yuxia Luan
Docetaxel (DTX) can produce anti-tumor effects by inhibiting cell growth and inducing apoptosis. However, the poor solubility of DTX restricts its application and its clinical formulation has caused serious adverse reaction due to the use of Tween-80. In the present study, DTX was conjugated to an amphiphilic di-block polymer to solve these problems. Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (mPEG-PCL) was selected as the polymer skeleton and a redox sensitive disulfide bond was used as the linker between DTX and mPEG-PCL. The synthesized mPEG-PCL-SS-DTX conjugates were characterized by 1 H-nuclear magnetic resonance ( 1 H NMR) and Fourier transform infrared spectroscopy (FTIR). Interestingly, the mPEG-PCL-SS-DTX conjugates could self-assemble into micelles in aqueous solution. The critical micelle concentration (CMC) of mPEG-PCL-SS-DTX micelles was about 2.3 mg L −1 determined using pyrene molecule fluorescent probe method while the size of mPEG-PCL-SS-DTX micelles was determined to be ca . 17.6 nm and 116.0 nm with a bimodal distribution by dynamic light scattering (DLS). The in vitro release results indicated that the as-prepared micelles exhibited a sustained release profile with good redox sensitive properties. In particular, the hemolytic toxicity test indicated the as-prepared mPEG-PCL-SS-DTX micelles had negligible hemolytic activity, demonstrating their safety in drug delivery system. Cytotoxicity assay of the mPEG-PCL-SS-DTX micelles verified their highly enhanced cytotoxicity to MCF-7/A and A549 cells. These results thus demonstrated that the present redox-sensitive mPEG-PCL-SS-DTX micelle was an efficient and safe sustained drug delivery system in the biomedical area.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
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DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2016
·Vol. 57
·期 2
·206-213
·
IF 1.719
Xiufang Li,Qiang Zhang,Dezhan Ye,Jie Zhang,Yuhang Guo,Renchuan You,Shuqin Yan,Mingzhong Li,Jing Qu
To engineer tissue restoration, it is necessary to provide a bioactive, mechanically robust scaffold. Electrospun poly(ε-caprolactone) (PCL) nanofiber is a promising biomaterial candidate with excellent mechanical properties, but PCL scaffolds are inert and lack natural cell recognition sites. To overcome this problem we investigated the incorporation of Antheraea pernyi silk fibroin (ASF) containing inherent RGD tripeptides with PCL in electrospinning process. The mixing ratios showed remarkable impact on the properties of hybrid nanofibers. Increasing PCL content significantly enhanced the mechanical properties of nanofibers. In particular, the mechanical properties were remarkably enhanced when PCL content increased from 50 wt% to 70 wt%. Moreover, the biological assays based on endothelial cells showed promoted cell viability when ASF content reached to 30 wt%. The data demonstrated that the nanofiber containing 70% of PCL and 30% of ASF achieved the most balanced performances for integrating the mechanical properties of PCL and the bioactivity of ASF. Furthermore, biomimetic alignment of 70PCL/30ASF nanofibers was achieved, which could support PC12 neuron-like cell growth and guide neurite outgrowth, providing a potentially useful option for the engineering of oriented tissues. The results show that the PCL/ASF hybrid nanofibers can be considered as a promising candidate for tissue engineering scaffolds. POLYM. ENG. SCI., 57:206–213, 2017. © 2016 Society of Plastics Engineers
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
材料科学
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DOI
高影响因子
BIOMATERIALS
·
2022
·
IF 15.304
Yaping Wang,Jingrong Wang,Rui Gao,Xiang Liu,Zujian Feng,Chuangnian Zhang,Pingsheng Huang,Anjie Dong,Deling Kong,Weiwei Wang
The reconstruction of large cranial bone defects by bioactive materials without exogenous cells or growth factors remains a substantial clinical challenge. Here, synthetic fibrous glycopeptide hydrogel (GR gel ) self-assembled by β-sheet RADA16-grafted glucomannan was designed to mimic the glycoprotein composition and the fibrillar architecture of natural extracellular matrix (ECM), which was non-covalently composited with 3D-printed polycaprolactone/nano hydroxyapatite (PCL/nHA) scaffold for cranial bone regeneration . The glycopeptide hydrogel significantly promoted the proliferation, osteogenic differentiation of bone mesenchymal stem cells (BMSCs), which was further augmented by GR gel -induced macrophage M2-phonotype polarization and the effective M2 macrophage-BMSC crosstalk . The repair of critical-size skull bone defect in rat indicated a superior efficacy of PCL/ [email protected] gel implant on bone regeneration and osseointegration , with an average bone area of 83.3% throughout the defect location at 12 weeks post treatment. Furthermore, the osteo-immunomodulatory GR gel induced a reparative microenvironment similar with that in normal cranium, as characterized by an increased percentage of anti-inflammatory M2 macrophages and osteoblasts, and high-level vascularization . Collectively, the composite scaffold developed here with macrophage polarization-mediated osteo-immunomodulation may represent a promising implant for expediting in situ bone regeneration by providing biochemical and osteoinductive cues at the injured tissue.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）