Clinical Oral Investigations
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2024
·Vol. 28
·期 10
·1-14
·
IF 3.1
Liu Runze,Liu Pei,Luo Yi,Fan Wei,Fan Bing
Objectives The mannose phosphotransferase system (Man-PTS) plays crucial roles in the adaptive metabolic activity of Enterococcus faecalis ( E. faecalis ) in adverse environments. The aim of this study was to evaluate the role of Man-PTS in the alkaline resistance of E. faecalis against calcium hydroxide (CH) and the effect of metformin (Met) on the alkaline resistance of E. faecalis to CH. Materials and methods The regulatory role of Man-PTS EII in the alkaline resistance of E. faecalis was firstly investigated using a wild-type highly alkaline-resistant E. faecalis XS 003, standard ATCC 29212 and Man-PTS EIID gene deficient (△ mptD ) and overexpressing (+ mptD ) strains of E. faecalis . RNA sequencing of Met-treated E. faecalis was performed to further validate the effect of Met on Man-PTS. The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of Man-PTS EII and membrane transporter-related genes of E. faecalis . The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested. The in vivo therapeutic effect of Met plus CH (CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis XS 003. Results Man-PTS EII significantly promoted the survival ability of E. faecalis in CH and enhanced its resistance to CH. The inhibition of Man-PTS EII by Met resulted in reduced alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. Additionally, Met plus CH showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusions Met could significantly reduce the alkaline resistance of E. faecalis against CH through the modulation of Man-PTS EII, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo. Clinical relevance Met could significantly enhance the ability of CH to control E. faecalis infection through reducing the alkaline resistance of E. faecalis .
研究领域:
微生物学
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药理学
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牙科学
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传染病学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yaozhi Zhang,Zhijia Wang,ShuChang Yao,Xiaoyu Lin,Xiang Zhang,Xinru Tan,Liuyang Zhang,Ran Xu,Yihang Zhao,Chen Zhao,Fuhao Chu,Wenguang Jing,Xuemei Huang,Penglong Wang
The global spread of Staphylococcus aureus ( S. aureus ) not only causes significant economic losses but also poses a serious threat to public health. Consequently, there is an urgent need for multidimensional strategies to develop antimicrobial dressings to combat bacterial infections. In response, we have developed a plant polysaccharide antibacterial hydrogel formed through the self-assembly of edible Kudzu powder (KP) and the natural star molecule berberine (BBR). Rheological tests show that natural polysaccharide KP-BBR hydrogel (BBR@KP) exhibits excellent injectability and adhesion. And the degradation of the hydrogel exceeded 90 % within 3 days. The synergistic effect of these two ingredients enhances the antibacterial activity of BBR and can increase the MIC of BBR to 0.05 mM. Specifically, KP promotes the affinity of the Phosphoenolpyruvate Phosphotransferase System (PTS) of S. aureus , enabling KP, with its bioadhesive properties, to adhere to the bacterial surface and continuously release BBR. Subsequently, BBR effectively exerts its antibacterial effect by inhibiting the synthesis of histidine and isoleucine. Furthermore, the BBR@KP hydrogel exhibits negligible cytotoxicity and hemolytic toxicity, underscoring its favorable biosafety profile. This synergistic natural antibacterial hydrogel, formulated through a green and straightforward methodology, not only holds promise for broad clinical applications but also provides novel perspectives for the utilization and development of plant polysaccharides in the biomedical field.
研究领域:
生物医学工程
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药学
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微生物学
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qiyilan Guang,Baoyu Huang,Jun Yu,Maryam Bonyani,Mohammad Moaddeli,Mansour Kanani,Ali Mirzaei,Hyoun Woo Kim,Sang Sub Kim,Xiaogan Li
In this paper, platinum sulfide decorated WS 2 microflakes (PtS/WS 2 ) heterostructure nanocomposites were prepared by an in-situ chemical reduction process. The chemiresistive-type gas sensor based on the synthesized PtS/WS 2 heterostructure nanocomposites exhibited a significantly improved sensing properties compared to that of the pure WS 2 microflakes at different concentration of NH 3 from 1 ppm to 10 ppm at room temperature . As the concentrations of PtS nanoparticles decorated on the surface of WS 2 microflakes increased, the response of the PtS/WS 2 heterostructure sensor to ammonia firstly increased when the concentration of PtS increased up to 1 wt%, whereas above 1%, the response decreased instead. Moreover, it was found that the response speeds of the PtS-decorated WS 2 were significantly improved compared to that pure WS 2 . The sensors also exhibited excellent selectivity to formaldehyde, toluene, acetone , methanol and ethanol at room temperature. The sensing mechanisms were studied by using the in-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). The enhanced sensing properties could be explained by “electronic sensitization” effect induced by the PtS/WS 2 heterostructure, being confirmed by density functional theory (DFT) calculations.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Xiaolan Yang,Jitao Li,Qiulan Li,Dezhi Yang,Yaling Yang
The amorphous Cu-containing phosphomolybdate (Cu-PTs) composite with high peroxidase (POD)-like activity at neutral conditions was explored as biosensors for raloxifene (RAF) detection. The strong attraction between negatively charged Cu-PTs and positively charged substrates 3,3′,5,5′-tetramethylbenzidine (TMB), as well as the acceleration of the conversion of active Cu + /Cu 2+ by the Cu/W bimetallic redox couples were demonstrated to play significant roles in POD-like activity in physiological environment. When RAF is presence, it can bind to the surface of Cu-PTs and changes the chemical signal on the material surface, leading to the decreased POD-like activity. Based on this, a colorimetric method was established for the sensitive assay of RAF with a lower limit of detection (LOD) of 0.025 mg/L and good recovery from 90.13% to 108.9%. This work paves a new way to design a POD-like colorimetric protocol for tracing RAF in pharmaceutical products and environmental samples.
研究领域:
分析化学
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生物传感
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环境科学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Jingqiu Sun,Xian Zhang,You Wu,Chengzhi Hu
Selective separation of nitrate over chloride is crucial for eutrophication mitigation and nitrogen resource recovery but remains a challenge due to their similar ionic radius and the same valence. Herein, a polypyrrole membrane electrode (PME) was fabricated by polymerization of pyrrole (Py) and p-toluenesulfonate (pTS), which was used as a working electrode in redox transistor electrodialysis . The anions in the source solution were first incorporated into the PME at reduction potentials and then released to receiving solution at oxidation potentials. Pulse widths and potentials were optimized to maximize the ion separation performance of PME, resulting in the improvement of NO 3 − /Cl − separation factor up to 6.93. The ion distributions in various depths of PME indicated that both NO 3 − and Cl − were incorporated into PME at negative potentials. Then, NO 3 − was preferentially released from PME at positive potentials, but most Cl − was retained. This was ascribed to the high binding energy between Cl − and PPy/pTS structure, which was 51.4% higher than that between NO 3 − and PPy/pTS structure. Therefore, the higher transport rate of NO 3 − in comparison with Cl − was achieved, leading to a high NO 3 − selectivity over Cl − . This work provides a promising avenue for the selective separation of nitrate over chloride, which may contribute to nitrogen resource recycling and reuse.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
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2025
·
IF 2.5
Zhao Jinwen,Wang Le,Liu Xiaolan,Zhu Yanqin,Yin Qinhong
Nanozymes have been valuable tools in colorimetric analysis due to their ease of production, long-term stability, and adaptable enzymatic activity, enabling them to induce color changes in substrates. This study presents a straightforward enzyme-based colorimetric sensor for morphine ( MOR ) detection. The sensor utilized the laccase-like activity of a self-synthesized Cu, Bi-phosphotungstic acid ( Cu, Bi-PTs ) nanozyme, which was loaded with phosphotungstic acid, copper, and bismuth through a one-pot microwave method. This nanozyme was capable of catalyzing the coupling reaction of 2,4-dichlorophenol ( 2,4-DP ) and 4-aminoantipyrine ( 4-AP ), leading to the conversion of colorless phenolic compounds into red-colored products. The method demonstrated a detection limit of 1.88 μg/mL for MOR and a linear dynamic range from 3.333 to 400 μg/mL. The calibration curve, given by the equation y = 0.0015x + 0.4081, exhibited excellent linearity (R 2 = 0.9914), confirming the method's reliability for quantitative analysis. The developed colorimetric method was successfully applied to real samples, showing relative standard deviations (RSD) between 1.4 and 4.3% for MOR detection. These results demonstrated that the method was sensitive, cost-effective, and rapid, making it suitable for the efficient determination of MOR .
研究领域:
纳米技术
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分析化学
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生物传感器
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环境与药物分析
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产品关联
DOI
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Bo Zhang,Li Chen,Jie-Yi Jin,Na Zhong,Xue Cai,Shu-Ping Zou,Hai-Yan Zhou,Zhi-Qiang Liu,Yu-Guo Zheng
: As the key precursor of coenzyme A synthesis in vivo , D-pantothenic acid plays an important role in maintaining biological function, which is widely applied in the food , pharmaceutical, and cosmetic industries. At present, it was mainly produced by chemical methods, with serious environmental pollution, therefore, it is urgent to develop an economical, practical, and environmentally friendly biological fermentation method based on microbial chassis cells. Herein, based on our previously developed D-pantothenic acid producing strains, we obtained higher yielding D-pantothenic acid producing strains by systematic metabolic engineering . First, according to the results of CRISPRi experiments, lpd were overexpressed, disrupted the non-PTS (non-phosphoenolpyruvate-pyruvate transport system) as well as enhanced the PTS (phosphoenolpyruvate-pyruvate transport system) allowing precursor accumulation. Subsequently, based on the analysis of metabolic intermediates , the pyruvate bypass pathway was blocked as well as the (R)-pantothenic acid pathway was further enhanced, increasing the titer of D-pantothenic acid to 4.60 g/L. Finally, the organic acid and branched-chain amino acid synthesis pathways were blocked, and in order to accelerate the synthesis of acetolactate and the output of D-pantothenic acid, heterologous acetolactate synthase and pantothenate transfer protein were introduced, which significantly increased the yield of D-pantothenic acid, reach to 6.33 g/L. The final strain in a 5 L bioreactor produced 32.32 g/L D-pantothenic acid with a productivity of 2.33 g/L/h and the high-yielding strain provides an effective platform for biological fermentation to produce D-pantothenic acid.
研究领域:
生物技术
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代谢工程
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微生物学
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发酵技术
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产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·
IF 2.8
Lin Lin,Xu Liu,Ce Shi,Xiaochen Chen,Tariq Aziz,Fahad Al-Asmari,Amal A. Mohamed,Haiying Cui
The objective of this research was to develop and analyse a chitosan- Tremella fuciformis polysaccharide (CS-TFP) edible film using chitosan (CS) and Tremella fuciformis polysaccharide (TFP) as the primary raw materials. The findings revealed significant improvements in the thickness, solubility, opacity, mechanical properties and barrier properties (water vapour and oxygen permeability) of the chitosan film upon the addition of TFP, but water content and hydrophobicity were reduced. Moreover, TFP addition markedly enhanced the antibacterial and antioxidant activities of the chitosan films. Structural analysis of the CS films and CS-TFP edible films indicated that the formation of hydrogen bonds and high compatibility between CS and TFP. Specifically, TFP increased crystallinity, reduced roughness and improved the compactness of the edible film. This increase in compactness was directly linked to enhanced physical properties, whereas increased crystallinity contributed to better thermal stability. Furthermore, the application of CS-TFP-3 edible films in chicken preservation proved that they could significantly delay the deterioration of chicken. Findings in this study suggested that CS-TFP edible film as a bioactive packaging material showed great potential to preserve chicken meat and extend the shelf life.
研究领域:
食品科学
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材料科学
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DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·
IF 2.8
Yang Zou,Yanfen Zhang,Hui Zhang,Bin Cao,Zixin Luo,Huawei Duan
Studies have shown that fluoridated reagents for oil and water resistance are harmful to the human bodies and the environment. Consequently, the developments of nontoxic, environmentally friendly, fluorine-free and degradable materials have received increasing attentions. Here, we introduce a novel method for fabricating a hydrophobic and oleophobic paper-based packaging material through the application of chitosan/carnauba wax emulsions. These emulsions were prepared via high-shear homogenization, blending melted carnauba wax with chitosan in acetic acid aqueous solutions. The stable chitosan/carnauba wax emulsion with particle size of 430 nm was prepared at the chitosan concentration of 1.5 wt%, the carnauba wax concentration of 8 wt% and temperature of 85 °C. After 10 g/m 2 of chitosan/carnauba wax emulsion was applied to the paper surface, the oil and water resistance of the paper was significantly improved. The experimental results of kit rating value, oil contact angle, Cobb 60 value and water contact angle were 9.5 ± 0.6, 86.6 ± 2.1°, 8.90 ± 1.62 g/m 2 and 123.5 ± 2.6°, respectively. The application of chitosan/carnauba wax emulsion coating on papers provided a significantly lower water vapour transmission rate (WVTR, 78.45 ± 18.92 g/m 2 ·24 h) and oxygen transmission rate (OTR, 94.26 ± 20.21 g/m 2 ·24 h) as compared to the original paper, which met the needs of strawberry packaging.
研究领域:
材料科学
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环境科学
·
食品科学
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产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·Vol. 37
·期 8
·793-807
·
IF 2.8
Haiying Cui,Jie Gao,Myat Noe Khin,Tariq Aziz,Fahad Al-Asmari,Abdulhakeem S. Alamri,Majid Alhomrani,Lin Lin
A pH-sensitive protein fibre membrane was prepared using alizarin (ALI), curcumin (CUR), gelatin, zein and cinnamaldehyde as raw materials, by electrospinning technology. The membrane was developed to detect food deterioration and extend shelf life. It was observed that the addition of CUR significantly enhanced the antioxidant and barrier properties of the membrane. The fibre membrane was responsive to pH with minimal colour alteration in response to humidity (from 20% to 80% relative humidity) and demonstrated excellent reusability along with a low detection limit for ammonia gas. Moreover, it was capable of monitoring low concentrations of bacteria in the headspace. To assess the efficacy of the membrane, a freshness monitoring and preservation study was carried out on pork stored at 4°C for 5 days. The fibre membrane not only prolonged the shelf life of meat but also allowed real-time monitoring of its freshness. The results highlight the potential application of the prepared nanofibres in the simultaneous monitoring and preservation of meat freshness.
研究领域:
食品科学
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材料科学
·
生物技术
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产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·Vol. 37
·期 8
·745-754
·
IF 2.8
Zhuolin Liu,He Wang,Jiaxin Yi,Wenya Ma,Xiaofang Liu,Xiaohan Chen,Ying Cao,Li Li
A functional edible film containing Pickering emulsion (PE) loaded with oregano essential oil (OEO) was developed. The sustained release of OEO in muffins significantly delayed muffin decay at 25°C ( p < 0.05). According to the influence of oxygen permeability, antibacterial activity and mechanical properties of the active film, the optimum preparation conditions of PE were determined by orthogonal experiment. Peroxide values (POVs), total viable count (TVC) and yeast and mould count (YMC) were significantly lower compared with the blank group. On Day 6, the POV in the experimental group was 63% that of the blank group. TVC and YMC were 3.20 and 1.86 lg CFU/g on Day 9, respectively (TVC ≤ 1500 CFU/g; YMC ≤ 100 CFU/g), extending muffins shelf life to 6–7 days in practical preservation at 25°C. Thus, the edible PE loaded with OEO film provided sustained release, effectively slowing muffin quality deterioration and extending shelf life, making it a promising choice for future high-fat food packaging.
研究领域:
食品科学
·
生物学
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产品关联
DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2026
·
IF 4.7
Qingfeng Dong,Yuqing Ye,Xiao Wang,Xiaofang Liu,Shuting Huang,Jinbin Wang,Li Li
In this study, the cellulose acetate phthalate (CAP) film loaded with tannic acid (TA) was prepared in the work using the electrospinning method. The addition of TA increased both the conductivity and the viscosity of the electrospinning solution, which resulted in a higher average fibre diameter (1.14 ± 0.30 to 1.32 ± 0.32 μm). The electrospun films exhibited antibacterial activity, with the highest inhibition zone diameter values of 8.3 mm against Escherichia coli and 8.5 mm against Staphylococcus aureus , as well as antioxidant activity with a maximum free radical scavenging rate at 41%. The combined benefits of TA-loaded electrospun CAP film and vacuum skin packaging on the quality of minced beef were investigated. It has been found that the microbial, physical and chemical deterioration of the minced beef was effectively retarded; hence, it extended its shelf life by an extra 2 days. In conclusion, the TA-loaded electrospun CAP film is highly effective in preserving the quality of minced beef using vacuum skin packaging.
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高影响因子
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