CURRENT ISSUES IN MOLECULAR BIOLOGY
·
2026
·Vol. 48
·期 5
·494
·
IF 4.1
Daoyuan Li,Jie Wang,Naifu Chen,Naidong Chen
To evaluate the immune-enhancing effects of Polygonatum cyrtonema polysaccharides in vivo, an immunodeficiency zebrafish model was established by microinjecting vinorelbine tartrate into the caudal vein. Effects of the polysaccharides (500, 1000 and 2000 μg/mL) on neutrophil counts were assessed in Tg (mpx:GFP) zebrafish. Transcriptome sequencing was employed to investigate the immunomodulatory effects of the polysaccharides. The results revealed a dose-dependent increase in neutrophil counts following treatment with the polysaccharides. Transcriptomic profiling identified 1286 DEGs across the three comparison groups. GO and KEGG enrichment analyses indicated that the polysaccharides could modulate immune-related pathways in the zebrafish model. Two enriched KEGG pathways, including the MAPK signaling and the mTOR signaling pathway, were utilized to analyze immune-related gene expression. To validate RNA-seq data, qRT-PCR was performed on selected DEGs, including il1b , crk , fgf10b , atp6v1aa , and eif4e1c . The results confirmed that the expression patterns of these genes were consistent with the RNA-seq data. Within the tested concentrations (500, 1000 and 2000 μg/mL), the polysaccharides exhibited a dose-dependent immunostimulatory effect, with the highest immunostimulatory response observed at 2000 μg/mL. The molecular level primarily involves the enhancement of neutrophil function through the modulation of multiple immune-related pathways. These findings provide a theoretical basis for the potential application of Polygonatum cyrtonema polysaccharides as a natural immunomodulatory agent.
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JOURNAL OF PHARMACEUTICAL SCIENCES
·
2024
·
IF 3.7
Dong Liu,Tingting Hou,Chunye Geng,Lu Song,Xuefeng Hou,Yanjun Chen,Fang Wang,Wei Wang,Bangxing Han,Leilei Gao
Poor stability and difficult uptake of natural polysaccharides have been the main problems in their application. The purpose of this study was to optimize the preparation conditions of Polygonatum cyrtonema Hua polysaccharides liposomes (PCPL) and to investigate the immune enhancement activity of PCPL in vitro and in vivo, with a view to discovering new ways of natural polysaccharide application. The optimal preparation conditions of PCPL were as follows: the adding amount of Tween 80 of 0.5 %, the ultrasound time of 2 min and the ultrasound times of once. Under these conditions, the entrapment efficiency, drug loading rate and particle size of PCPL were 38.033 %±0.050, 2.172 %±0.003 and 146 nm, which indicated that PCPL with small particle size could be prepared by the reverse-phase evaporation method. Furthermore, PCPL promoted proliferation, phagocytosis, and secretion of nitric oxide and related cytokines in RAW264.7 cells. Moreover, PCPL improved spleen and thymus indices, increased the number or proportion of red blood cells, platelets, and lymphocytes in the blood, and ameliorated spleen and thymus atrophy in immunosuppressed mice. This study provides a new idea for applying Polygonatum cyrtonema Hua polysaccharides (PCP) and references for studying other polysaccharides.
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MOLECULES
·
2026
·Vol. 31
·期 19
·3542
·
IF 5.1
Xueqing Geng,Xingchi Zhang,Yun Wang,Ting Yao,Zhuoting Gan
Polygonatum cyrtonemaHua in Jiuhua Mountain (JHPCH) is a nationally recognized geographically indicated medicinal plant known for its bioactive polysaccharides with diverse pharmacological activities. This study isolated a homogeneous polysaccharide JPCP-70-0 from JHPCH via hot water extraction, ethanol precipitation, and chromatographic purification. Structural characterization by GC-MS, FT-IR, and NMR identified it as a neutral fructan (2.88 × 104Da) with Glc/Fru (6.65%/93.35%), a backbone of →1)-β-D-Fruf-(2→, and side chains of →6)-β-D-Fruf-(2→). Using Caenorhabditis elegans as a model, we evaluated JPCP-70-0’s effects on nematode growth. Results showed concentration-dependent regulation: low concentrations promoted growth, locomotion, feeding, and reproduction, while high concentrations induced developmental inhibition and reduced activity. This work provides a theoretical basis for the rational development of JHPCH resources.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ling Li,Kiran Thakur,Bu-Yan Liao,Jian-Guo Zhang,Zhao-Jun Wei
We examined the antioxidant and antimicrobial attributes of Polygonatum cyrtonema Hua polysaccharides (HBSS, CHSS, DASS, CASS). The UV spectra and FT-IR results showed the purity and acidic nature of polysaccharides with β -configurations. DASS (78.585% hydroxyl radical scavenging, 92.794% ABTS radical scavenging, 75.648% DPPH radical scavenging activity, 0.582 reducing power and 98.721% Fe 2+ chelation at 5 mg/mL) possessed the best antioxidant potential; while, HBSS (21.113% hydroxyl radical scavenging, 14.412% ABTS radical scavenging, 28.005% DPPH radical scavenging activity, 0.285 reducing power and 48.969% Fe 2+ chelation at 5 mg/mL) was observed with the lowest antioxidant action. The bacterial inhibition effect of four PCHPs on Gram-positive organisms ( B. subtilis and S. aureus with the maximum inhibitory diameters of 18.9 and 15.8 mm, respectively) was better than that of Gram-negative bacteria ( E. coli and S. typhimurium with the maximum inhibitory diameters of 10.9 and 8.4 mm, respectively). The bacterial sensitivity was observed in the following order: B. subtilis > S. aureus > E. coli > S. typhimurium . The trend of antibacterial action of PCHPs was in following order: DASS > CHSS > CASS > HBSS. DASS can be considered as natural anti-oxidant as well as spoilage inhibitors to enhance the nutritive and functional values of various foods.
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MOLECULES
·
2024
·Vol. 29
·期 9
·2076
·
IF 4.2
Ruiding Wen,Lu Luo,Runcheng Zhang,Xudong Zhou,Wei Wang,Limin Gong
A neutralPolygonatumcyrtonemapolysaccharide (NPCP) was isolated and purified fromPolygonatumcyrtonemaby various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
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Food & Function
·
2022
·Vol. 13
·期 24
·12883-12895
·
IF 6.317
Wei Liu,Taili Shao,Lei Tian,Zhengrui Ren,Lan Gao,Zhiyan Tang,Zheng Fang,Pingchuan Yuan,Chunyan Liu,Jikun Li,Guodong Wang,Jun Han
The chemical structure and pharmacological activity of Polygonatum cyrtonema Hua polysaccharides have garnered significant attention in recent years. In this study, a homogeneous polysaccharide, PCP1, was extracted from P. cyrtonema Hua rhizomes and purified. Monosaccharide composition analysis showed that PCP1 is primarily composed of fructose, glucose, and mannose. Chemical structure analysis showed that the main chain of PCP1 is composed mainly of →1)-β-D-Fruf-(2→ and →1,6)-β-D-Fruf-(2→, with small amounts of →6)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→, and β-D-Glcp-(1→. The side chain is β-D-Fruf-(2→ linked at C-6 of →1,6)-β-D-Fruf-(2→. In vivo experiments showed that PCP1 mitigates liver pathological damage, improves abnormal lipid metabolism and oxidative stress, promotes the production of short-chain fatty acids, and balances the composition of the intestinal microbiota in non-alcoholic fatty liver disease (NAFLD) mice. Thus, PCP1 can be used as a natural ingredient in functional foods for the treatment of NAFLD.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Junyin Zhang,Hulan Chen,Lan Luo,Zhipeng Zhou,Yingxiang Wang,Tianyu Gao,Lian Yang,Teng Peng,Mingyi Wu
Polygonatum cyrtonema is a known tonic herb in Chinese Materia Medica , extensively consumed in China, but the structure and activity of its polysaccharide components remain to be clarified. Herein, two new polysaccharides (a fructan and a galactan) were purified from the dried and the processed P. cyrtonema rhizome, respectively. Structural analysis suggested that the fructan consisted of a (2 → 6) linked β- d -Fru f residues backbone with an internal α- d -Glc p residue and two (2 → 1) linked β- d -Fru f residues branches, and that the galactan was a (1 → 4)-β- d -galactan branched with a single β- d -galactose at C-6 at about every nine residues in its main chain. The bioactive assay showed that the fructan and the galactan remarkably promoted growth of Bifidobacterium and Lactobacillus strains, indicating that they possess prebiotic activity. These findings may help expand the application of the polysaccharides from the tonic herb P. cyrtonema as functional ingredients in food products .
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Frontiers in Nutrition
·
2023
·
IF 5
Zhao Yuanyuan,Wang Zhen,Fu Ranze,Xie Ruonan,Wang Bin,Li Qinglin
Introduction: Polygonatum cyrtonema Hua. (PC) is a traditional Chinese herb with a history of use in both food and medicine. For clinical use, processed PC pieces are most commonly used, while present research has focused on crude PC polysaccharides (PCPs).Methods: In this study, a new polysaccharide, PCP-F1, with a molecular weight of 37.46 kDa, was separated from four-time processed PCPs by column chromatography and evaluated by antioxidant activity. It was composed of glucose, mannose, galactose, rhamnose, and galacturonic acid with a molar ratio of 3.5: 2.5: 1.3: 1.8: 0.8.Results and Discussion: The methylation analysis and two-dimensional NMR measurement revealed that the configuration of PCP-F1 contained nine residues in the primary structural unit by the chain of →3)-α-D-Glcp, →2)-α-D-Glcp (6→, →1)-ꞵ-D-Glcp (2→, →2)-α-D-GalAp (3,4→, →1) -ꞵ-D-Manp (3→, →2)-α-D-Glcp (3→, branched for →3)-α-D-Glcp, →2)-ꞵ-D-Galp (4→, →1)-ꞵ-D-Glcp (2→, →2,4)-α-D-Manp (6→, →3)-α-L-Rhap (4→. Radical scavenging assays indicated that PCP-F1 could scavenge radicals with a high scavenging rate, suggesting PCP-F1 possesses good antioxidant activity. The study confirms the importance of processed PC and offers the potential for exploiting it as a functional food.
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Food Bioscience
·
2024
·
IF 4.8
Yanyan Zhang,Shengjun Ma,Haixia Li,Peizi Liu,Keyi Chen,Wei Cai
Research on Polygonatum cyrtonema Hua has focused extensively on its main active components, yet antioxidant peptides remained less explored. This study optimized the preparation process of antioxidant peptides from P. cyrtonema via protein hydrolysis using response surface methodology. The peptide mixture was separated into four fractions using ultrafiltration and G-25 gel chromatography, based on antioxidant capacity. Notably, the F2 exhibited significantly higher Fe 3 ⁺ reducing power (17.17%) and ABTS· radical scavenging rate (30.1%) compared to other fractions at the same concentration. Characterization by Nano-LC-Q Exactive Plus quadrupole Orbitrap MS revealed that F2 contained 11,704 peptides, markedly more than the endogenous (2,454) and crude protein peptides (2,334). Bioinformatics and molecular docking identified three peptides—QFLPF, FVPF, and FPNLF—as having the highest antioxidant potential. These peptides increased cell viability in oxidative damage-induced HepG2 cells by 6.39%, 14.51%, and 9.98%, respectively, compared to the model group. This study lays the groundwork for future mechanistic investigations into the antioxidant peptides of P. cyrtonema.
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Food Bioscience
·
2024
·
IF 4.8
Yueyao Gui,Bing Zheng,Hairong Huang,Jianhua Xie,Yi Chen,Jialuo Shan,Xingxing Chang,Xiaobo Hu,Qiang Yu
Alcoholic liver disease (ALD) is characterized by inflammation and oxidative stress induced by excessive alcohol consumption. It is necessary to find a way to use natural active substances to alleviate the liver damage caused by alcohol. The object of this study was to investigate the beneficial effects of pure Polygomatum cyrtonema polysaccharide (PCP-80%) on ALD, and to explore its possible mechanism from the aspect of oxidative stress, inflammation and intestinal microbiota. The results demonstrated that PCP-80% effectively reduced serum biochemical index level and improved liver disease induced by acute alcohol exposure. Furthermore, PCP-80% showed promising outcomes in mitigating oxidative stress and liver inflammation associated with ALD. This might relate to the inhibition of NF-κB pathway and the activation of Keap1/HO-1 pathway. Additionally,PCP-80% enhanced the intestinal barrier and elevated the levels of propionic acid in the colon contents. It also partially reversed the alcohol-induced disturbances in the intestinal microbiome. Overall, PCP-80% exhibited significant therapeutic potential in alleviating acute alcoholic liver injury by reducing oxidative stress, inflammation, and relieving disturbances in the intestinal microbiome. These findings presented a novel approach for the treatment of acute alcoholic liver injury, offering prospects for improving disease prognosis.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jihui Gao,Tian Zheng,Yuyang Shi,Lei Jiang,Lei Huang,Bingsheng Qiu,Yongxin Li,Gang Chen,Jinping Si,Huqing Yang,Shangbin Bai
L. plantarum P5 improved the fermentation performance of PC-enriched FRCs. • L. plantarum P5 - assisted fermentation reduced off-flavors in PC-enriched FRCs. • The PCL group exhibited slower moisture migration, delaying starch retrogradation. • The PCL group predominantly formed V-type complexes, reducing starch digestibility. Although Polygonatum cyrtonema (PC) is rich in bioactive compounds, its direct incorporation may compromise the sensory quality of fermented products. This study systematically investigated the effects of PC on fermented rice cakes (FRCs), using Lactobacillus plantarum P5-assisted fermentation as a strategy to improve product quality. L. plantarum P5, as the optimal strain, significantly promoted yeast growth and increased total soluble solids in fermented glutinous rice, thereby improving the specific volume, height-to-diameter ratio, and textural characteristics of PC-enriched FRCs. SPME-GC–MS analysis showed that P5-assisted fermentation reduced off-flavor compounds, including hexanal and (E)-2-heptenal. Additionally, the group containing both PC and L. plantarum P5 (PCL) exhibited a slower staling rate and reduced moisture migration during storage. The resistant starch (RS) content in the PCL group significantly increased from 17.94% to 20.17%. X-ray diffraction analysis revealed that the PCL group exhibited predominantly V-type crystalline structures, indicating the development of inclusion complexes between bioactive compounds in PC and starch. Meanwhile, the PCL group also showed enhanced hydrogen bonding between PC polysaccharides (PCPs) and starch chains, promoting starch aggregation and the development of a more compact gel network. Overall, this study provides an approach for incorporating PC into fermented cereal products with enhanced product quality and reduced starch digestibility. Download: Download high-res image (363KB) Download: Download full-size image
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Agriculture-Basel
·
2023
·Vol. 13
·期 9
·1820
·
IF 3.6
Jiabei Xia,Xingyu Mei,Huan Cheng,Shiguo Chen,Xingqian Ye,Jianle Chen
The rhizome ofPolygonatum, which has been consumed in China for nearly 2000 years, is rich in oligosaccharides, polysaccharides, saponins, and alkaloids and has various effects such as lowering blood sugar and anti-aging. Its aerial parts are often discarded as by-products and have been rarely studied; moreover, the nutritional composition and carbohydrate composition of these parts are not clear. In this study, the nutritional composition, amino acid evaluation, and the total phenolics content of the leaves, stems, fruits, and flowers ofPolygonatum cyrtonemaHua were analyzed, and the composition and content of carbohydrates were determined by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD). The results suggested that the leaves and flowers ofPolygonatum cyrtonemaHua (PC) contained 1.12–1.37% phenolic substances. The proteins of the aerial parts had high nutritional values, and the total amino acid content of the leaves and flowers, with the total leaf amino acids amounting to 11.77%, were followed by the flowers at 11.65%. The sugars in the flowers were mainly free monosaccharides at 10.38%, and the fruits were dominated by 9.44% fructo-oligosaccharides. This study provided new evidence for the utilization of the aerial parts ofPolygonatum.Keywords:Polygonatum cyrtonemaHua;nutrient composition;amino acid evaluation;composition of carbohydrate
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