ACS Synthetic Biology
·
2021
·
IF 5.11
Xinyi Cheng,Lu Pu,Shengwei Fu,Aiguo Xia,Shuqiang Huang,Lei Ni,Xiaochen Xing,Shuai Yang,Fan Jin
Bacterial pathogens operate by tightly controlling the pathogenicity to facilitate invasion and survival in host. While small molecule inducers can be designed to modulate pathogenicity to perform studies of pathogen–host interaction, these approaches, due to the diffusion property of chemicals, may have unintended, or pleiotropic effects that can impose limitations on their use. By contrast, light provides superior spatial and temporal resolution. Here, using optogenetics we reengineered GacS of the opportunistic pathogen Pseudomonas aeruginosa, signal transduction protein of the global regulatory Gac/Rsm cascade which is of central importance for the regulation of infection factors. The resultant protein (termed YGS24) displayed significant light-dependent activity of GacS kinases in Pseudomonas aeruginosa. When introduced in the Caenorhabditis elegans host systems, YGS24 stimulated the pathogenicity of the Pseudomonas aeruginosa strain PAO1 in a brain–heart infusion and of another strain, PA14, in slow killing media progressively upon blue-light exposure. This optogenetic system provides an accessible way to spatiotemporally control bacterial pathogenicity in defined hosts, even specific tissues, to develop new pathogenesis systems, which may in turn expedite development of innovative therapeutics.
研究领域:
微生物学
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分子生物学
·
传染病学
·
合成生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Qianqian Chen,Li Yu,Huifen Zhou,Haofang Wan,Yangyang Zhang,Haitong Wan,Jiehong Yang
The Box-Behnken design-response surface methodology (BBD-RSM) and Back-propagation neural network prediction model optimized by Simulated annealing (SA-BPNN) were used to optimize the extraction process of Astragali radix - Paeoniae radix alba by the green natural deep eutectic solvent (NADES), and the antioxidant activity of them was discussed. The best NADES for extracting Astragali radix - Paeoniae radix alba need to be screened out. The comprehensive evaluation value of the extraction yield was used as the index. The BBD-RSM and SA-BPNN were established for prediction and optimization. The antioxidant activity of Astragali radix - Paeoniae radix alba in vitro and in vivo was studied by detecting 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate and ferric reducing antioxidant power (FRAP) and the expression levels of SOD, MDA and GSH-Px in rat plasma. The results showed that the best NADES formula screened was Betaine-Urea (Bet-Ur). Compared with the BBD-RSM, the SA-BPNN had a higher degree of fitting, the comprehensive evaluation value was higher, and the error between tested and predicted value was smaller, which was more suitable as a prediction model for the extraction yield of Astragali radix - Paeoniae radix alba . Through the determination of antioxidant kits and oxidative factors, the results showed that Astragali radix - Paeoniae radix alba had antioxidant activity in vitro and in vivo . The study on the extraction process optimization and antioxidant activity of Astragali radix - Paeoniae radix alba were expected to provide a theoretical basis for its effective utilization.
研究领域:
药学
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分析化学
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生物技术
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药理学
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DOI
高影响因子
Microorganisms
·
2022
·Vol. 10
·期 3
·565
·
IF 4.926
Hao Tan,Delong Kong,Qingyun Ma,Qingqing Li,Yiqing Zhou,Xu Jiang,Zhiye Wang,Rebecca E. Parales,Zhiyong Ruan
In this study, theCutaneotrichosporon dermatisstrain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 106cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.Keywords:tetracycline;biodegradation;Cutaneotrichosporon dermatis;response surface methodology (RSM);degradation products;antibacterial potency
研究领域:
环境科学
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微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Yang Li,Wei Zhao,Anquan Yang,Min Xie,Ranhua Xiong,Chaobo Huang
Syzygium jambos (L.) Alston fruits, a traditional medicinal species in the Myrtaceae family, are rich in polyphenolic compounds. This study optimized polyphenol extraction from S. jambos fruits using ultrasonic-assisted extraction combined with response surface methodology, achieving a total polyphenol content (TPC) of 31.24 mg/g. To further enrich polyphenols, crude extracts (CEs) were fractionated using solvents of increasing polarity (petroleum ether, dichloromethane, and ethyl acetate). Results showed that the ethyl acetate phase (EAP) had the highest TPC (96.25 mg/g, threefold higher TPC than CE) and strongest antioxidant activity. In addition, polyphenol extracts demonstrated excellent biocompatibility, significant tyrosinase inhibition, and antibacterial activity against Escherichia coli and Staphylococcus aureus , with inhibition zones of 16.2 ± 0.5 and 14.3 ± 0.6 mm at 100 mg/mL. Finally, 41 polyphenolic compounds were identified by liquid chromatography–mass spectrometry (LC–MS) in EAP, including flavonoids (quercetin, isorhamnetin, and rutin), phenolic acids (caffeic acid and ferulic acid), and tannins (corilagin). These results demonstrate the potential of S. jambos polyphenols as natural additives for antioxidant, skin-whitening, and antimicrobial applications. Graphical The study, for the first time, systematically explored the impact of different polarity solvents on the distribution of polyphenols and their antioxidant and skin-whitening activities in the fruits of Syzygium jambos . Through the integration of cellular experiments and zebrafish embryo studies, the biocompatibility was comprehensively assessed.
研究领域:
植物学
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植物化学
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食品科学
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药理学
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 7
·2919-2930
·
IF 3.094
Gu Yuanjie,Guo Jianming,Zhou Xin,Xu Yong
Xylo-oligosaccharides have great value in food, feed fields. Previous studies have shown that organic acids catalyze the hydrolysis of xylan-rich sources for the production of xylo-oligosaccharides. In this study, gluconic acid of aldonic acid generated xylo-oligosaccharides via hydrolysis of xylan from corncob. In order to maximize efficiency of xylo-oligosaccharides production, the optimum conditions was ascertained by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum xylo-oligosaccharides yield of 57.73% using 4.6% gluconic acid at 167 °C for 28 min, which was similar to the predicted value and fitted models of xylo-oligosaccharides production. The results showed that the reaction temperature was crucial to xylo-oligosaccharides production, and by-product yields (xylose and furfural) could be effectively controlled by both reaction temperature and time. In addition, 44.87 g/L XOS was achieved by decreasing the solid-liquid ratio. Overall, the described process may be a preferred option for future high concentration xylo-oligosaccharides production.
研究领域:
生物化学
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食品科学
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酶工程
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DOI
高影响因子
Journal of Computational and Theoretical Nanoscience
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2016
·Vol. 13
·期 4
·2269-2276
·
IF 1.666
Qiu Jing-Wen,Zheng Xue-Song,Ma Bao-Di,Xu Yi
A nitrilase from Acidovorax facilis 72W for efficient converting aliphatic dinitriles to cyanocarboxylic acids was over-expressed in E. coli BL21(DE3). The culture conditions were optimized by Response Surface Methodology (RSM) to improve the production of target enzyme
in this study. The optimized result is that starch concentration 23.55 g/L, corn paste and yeast extract 20.15 g/L (1:1), and IPTG 0.18 mM. The model was confirmed by validating experiments and the obtained nitrilase activity (202.0 U/L) was very close to the predicted activity (201.9 U/L).
The specific nitrilase activity under optimized media is 1.75 times higher than that in LB media. For 1 M substrate, the conversion was increased from 70% to 97% after 6 h reaction by changing cells cultivated from initial LB media to optimal media.
研究领域:
生物过程工程
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酶技术
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分子生物技术
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微生物发酵
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应用微生物学
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DOI
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2021
·Vol. 307
·期 3
·2100745
·
IF 4.367
Ruisen Shi,Xueru Sheng,Haiyuan Jia,Jian Zhang,Na Li,Haiqiang Shi,Meihong Niu,Qingwei Ping
Transparent bamboo (TB) is an attractive biomass-derived material. However, the interrelation between process parameter and product performance is not clear yet. In this paper, the process parameter during preparation is optimized by orthogonal experiment and finally TB with excellent optical and mechanical properties is successfully prepared. Among the investigated runs, TB-12 exhibited the best performance with high transmittance (89.2%), good color difference property (L: 76.8, A: 5.4, B: 5.9), and excellent tensile strength (119.3 MPa). As the final aim of this work, deeper insight into the interrelation between process parameters and product performance is also investigated by response surface methodology (RSM) and linear analysis method. Based on the analysis result, it can be concluded that i) X 2 - temperature is the most influential factor to the lignin content ( p -value = 0.0532) during delignification process; ii) during resin impregnation process, lignin content has strong correlation with optical property: for example, transmittance ( R 2 = 0.90423) and A value ( R 2 = 0.92073), but has no significant correlation with mechanical property (tensile strength: R 2 = 0.04186). Moreover, it is found that the correlation between lignin content and transmittance can be preliminarily predicted before the experimental start. This hypothesis has been validated by importing experimental data of previous references into the model. This work demonstrates a deep analysis of TB preparation process and may provide the referenced gist for parameter selection.
研究领域:
材料科学
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生物质工程
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产品关联
DOI
高影响因子
CHEMICAL PAPERS
·
2025
·
IF 2.5
Huo Zhanpeng,Sui Zifeng,Zhang Zifeng,Lyu Qianmei,Hu Wenjie,Wu Yongfu,Li Ke
KOH-activated hydrochar (HC) prepared using biomass straw serves as a promising carbon dioxide (CO 2 ) adsorbent. However, numerous factors influence its preparation process, leading to significant variability in the adsorption performance of the resulting products. However, thoroughly understanding and optimizing this process remains challenging. In this study, a response surface method (RSM) and machine learning model were applied to predict the CO 2 adsorption capacity of potato straw active HC and analyze the influence of multiple factors on the CO 2 adsorption capacity. The results showed that the random forest (RF) model had a good predictive effect on the CO 2 adsorption effect of active HC, compared with the RSM model, based on the central composite design experimental dataset and additional experimental data. In addition, the hydrothermal carbonization temperature had the most significant influence on the CO 2 adsorption performance among various preparation factors, based on analysis of the RSM and RF models. Through experimental characterization and analysis, we found that with an increase in hydrothermal carbonization temperature, more biomass components would be decomposed, with the degree of carbonization increasing accordingly, forming a stable aromatic structure. Subsequently, more micropores would be generated following KOH activation, thus increasing the CO 2 adsorption performance of active HC.
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 59
·33681-33687
·
IF 2.936
Xing Zhang,Jilun Yao,Wei Peng,Wensi Xu,Zhongguo Li,Chong Zhou,Zhendong Fang
Response surface methodology (RSM) was utilized to model and optimize the dichloroacetonitrile (DCAN) degradation process by UV/PMS. A quadratic function between DCAN degradation efficiency and three factors including dosage of PMS, UV power and retention time was obtained. The model fitted very well according to high the value of R2 (0.9919) and Adj-R2 (0.9814). Additionally, the analysis of variance showed the influence of factors on degradation efficiency followed: retention time > UV power > dosage of PMS. Finally, the optimum conditions were suggested under this model. The degradation efficiency reached the maximum value of 96.2% with the optimum conditions: dosage of PMS = 0.2 mM, UV power = 7.95 W, retention time = 80 min.
研究领域:
环境科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Processes
·
2021
·Vol. 9
·期 7
·1116
·
IF 2.847
Xingxing Ye,Yexun Shi,Liming Shen,Peng Su,Ningzhong Bao
The “Drying-redissolution” method is promising for the industrial production of high-concentration well-dispersed graphene oxide slurry (GOS). As the potential key step in this method, the spray drying process requires a statistical investigation which guides the large-scale preparation of graphene oxide powder (GOP). This work systematically studies the effects of operating parameters, including nozzle airflow rate (439–895 L·h−1), atomization pressure (0.5–0.7 MPa), and liquid feed rate (3.0–9.0 mL·min−1), by using the response surface methodology integrated Box–Behnken design (RSM–BBD), aiming to produce GOP with high yield and easy re-dispersion. The optimized spray drying condition is predicted to be 439 L·h−1, 0.59 MPa, and 9.0 mL·min−1, at which a powder yield of 70.45% can be achieved. The experimentally obtained GOP has an average particle size of 11.65 μm and the low crumpling degree of the particle morphology results in the good re-dispersibility (97.95%) and excellent adsorption performance (244.1 mg·g−1) of GOP. The GOS prepared by the spray-dried GOP possess low viscosity and high exfoliation efficiency with a single-layer fraction up to 90.8%, exhibiting good prospects for application. This work first applied the RSM–BBD model on the spray drying process of GO, and evidenced the possibility of producing high-quality GO slurry with the “drying-redissolution” method.Keywords:graphene oxide;spray drying;response surface methodology;drying and redissolution
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2023
·
IF 5.1
Siyuan Yu,Yanting Zhou,Chunyue Zhao,Han Liu,Zhiyun Xue,Jinfeng Xing
Dispersion photopolymerization was carried out under green LED irradiation to rapidly prepare monodisperse poly(methyl methacrylate) (PMMA) microspheres with small size by using a high-efficient three-component initiating system. The polymerization parameters including exposure time, MMA content, stirring speed, water ratio and PVP content were systematically investigated. Crucial parameters were further optimized by response surface method (RSM), and a corresponding polymerization mechanism was proposed. Finally, PMMA microspheres with 375.6 nm of size and 0.101 of PDI were prepared. This study provides a facile method for the rapid fabrication of PMMA microspheres.
研究领域:
材料科学
·
高分子化学
·
纳米技术
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产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3438
·
IF 5.2
Chaoqun Sun,Guodong Wang,Jing Sun,Jiyong Yin,Jian Huang,Zizi Li,Di Mu,Menglu He,Tingting Liu,Jiali Cheng,Hanchen Du,Yan Chen,Wenjie Qu
Polygonatum sibiricumPolysaccharide (PsP) with antioxidant function is the main active component ofPolygonatum sibiricum(P.sibiricum). The currently poor extraction yield and extraction methods of PsP cannot meet the application of that in food industrial production. In this research, an ultrasound-assisted extraction-deep eutectic solvents (UAE-DESs) method, which has never been used in the PsP industry, was first used to extract PsP. The extraction conditions were optimized by the response surface method (RSM). Both the extraction yield and antioxidant function were simultaneously considered during the optimization process. The indicators of PsP’s level and antioxidant activity in vitro were used to present the extraction yield of the UAE-DESs method, the purity, and the antioxidant effect of PsP. Under the optimal conditions, which included a liquid–solid ratio of 26:1 (mL:g), extraction temperature of 80 °C, ultrasonic time of 51 min, and ultrasonic power of 82 W, the PsP extraction yield could reach (43.61 ± 0.09)%, which was obviously higher than single DESs (33.81%) and UAE (5.83%), respectively, and the PsP appeared favorably antioxidant function. This research proposed an efficient extraction method for PsP, filled the basic research gap, and further improved the development of PsP as a dietary supplement with antioxidant function in the food industry.Keywords:Polygonatum sibiricumPolysaccharide (PsP);ultrasound-assisted extraction-deep eutectic solvents (UAE-DESS);antioxidant function;response surface method (RSM)
研究领域:
食品科学
·
药学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）