Scientific Reports
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2023
·Vol. 13
·期 1
·1-14
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IF 4.6
Wen Dingxin,Guo Qingqing,Zhao Wan,Yang Yong,Yang Chunlei,Yu Jun,Hu Yun
Since its discovery as a third unique gaseous signal molecule, hydrogen sulfide (H 2 S) has been extensively employed to resist stress and control pathogens. Nevertheless, whether H 2 S can prevent tobacco bacterial wilt is unknown yet. We evaluated the impacts of the H 2 S donor sodium hydrosulfide (NaHS) on the antibacterial activity, morphology, biofilm, and transcriptome of R. solanacearum to understand the effect and mechanism of NaHS on tobacco bacterial wilt. In vitro, NaHS significantly inhibited the growth of Ralstonia solanacearum and obviously altered its cell morphology. Additionally, NaHS significantly inhibited the biofilm formation and swarming motility of R. solanacearum , and reduced the population of R. solanacearum invading tobacco roots. In field experiments, the application of NaHS dramatically decreased the disease incidence and index of tobacco bacterial wilt, with a control efficiency of up to 89.49%. The application of NaHS also influenced the diversity and structure of the soil microbial community. Furthermore, NaHS markedly increased the relative abundances of beneficial microorganisms, which helps prevent tobacco bacterial wilt. These findings highlight NaHS's potential and efficacy as a powerful antibacterial agent for preventing tobacco bacterial wilt caused by R. solanacearum .
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PLANT JOURNAL
·
2026
·Vol. 128
·期 1
·e71111
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IF 6.2
Bo Nian,Sai Yuan,Yijie Liu,Ying Zhang,Pengyue Li,Yang Lin,Jiatao Xie,Jiasen Cheng,Yanping Fu,Xiao Yu,Daohong Jiang,Bo Li
SUMMARY Bacterial wilt caused by Ralstonia solanacearum is a devastating disease that affects a wide range of crops, including tobacco. The pathogen promotes infection by secreting type III effector (T3E) proteins into host plants to facilitate pathogenicity; however, the molecular mechanisms for many of these effectors remain elusive. Here, we characterize RipAD, a conserved T3E that significantly contributes to R. solanacearum virulence in Nicotiana tabacum and Nicotiana benthamiana . We demonstrate that RipAD dampens early immune responses, including pathogen-associated molecular pattern-triggered reactive oxygen species (ROS) bursts and MAPK activation. This immunosuppressive function depends on the dual localization of RipAD to the plasma membrane and the nucleus. Mechanistically, RipAD physically associates with the N-terminal domains of the plasma membrane intrinsic proteins NbPIP2;3 and NbPIP2;4. We show that these aquaporins act as positive regulators of ROS accumulation and plant immunity. Crucially, RipAD binding is associated with reduced homo- and hetero-oligomerization of NbPIP2;3 and NbPIP2;4, thereby compromising their stability and function. Furthermore, silencing NbPIP2;3 and NbPIP2;4 increases tobacco susceptibility and rescues the virulence defect of a RipAD deletion mutant. These findings reveal a novel virulence strategy wherein a bacterial effector targets the structural integrity of host aquaporin complexes to block ROS signaling and suppress immunity.
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Viruses-Basel
·
2022
·Vol. 14
·期 11
·2431
·
IF 5.818
Jing Zheng,Wenyuan Chen,Hao Xiao,Fan Yang,Xiaowu Li,Jingdong Song,Lingpeng Cheng,Hongrong Liu
GP4, a newRalstonia solanacearumphage, is a short-tailed phage. Few structures ofRalstonia solanacearumphages have been resolved to near-atomic resolution until now. Here, we present a 3.7 Å resolution structure of the GP4 head by cryo-electron microscopy (cryo-EM). The GP4 head contains 540 copies of major capsid protein (MCP) gp2 and 540 copies of cement protein (CP) gp1 arranged in an icosahedral shell with a triangulation number T = 9. The structures of gp2 and gp1 show a canonical HK97-like fold and an Ig-like fold, respectively. The trimeric CPs stick on the surface of the head along the quasi-threefold axis of the icosahedron generating a sandwiched three-layer electrostatic complementary potential, thereby enhancing the head stability. The assembly pattern of the GP4 head provides a platform for the further exploration of the interaction betweenRalstonia solanacearumand corresponding phages.Keywords:Ralstonia solanacearumphage;icosahedral head;triangulation number T = 9;trimeric CP;Cryo-EM
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PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Qiulan Xia,Maoyang Ran,Lihe Zhou,Zhongwei Liu,Lin Cai
BACKGROUND Ralstonia solanacearum , a notorious and refractory bacterial plant pathogen, threatens multiple vegetable crops and causes significant economic loss in agriculture. Long-term use of traditional medicines not only increases the problem of drug resistance, but also causes great environmental pollution. Therefore, there is an urgent need to develop new agents with high efficacy and low toxicity. RESULTS In this study, we have synthesized and characterized graphitic carbon nitride incorporated copper oxide composite (g-C 3 N 4 @CuO), which showed higher antimicrobial effect than graphitic carbon nitride nanosheets (g-C 3 N 4 nanosheets) and copper oxide nanoparticles (CuONPs). Ralstonia solanacearum exposed to g-C 3 N 4 @CuO exhibited higher levels of oxygen toxicity, cell membrane damage, DNA damage, motility disruption and even cell death compared to g-C 3 N 4 nanosheets and CuONPs. In addition, g-C 3 N 4 @CuO was more effective in the control of tobacco bacterial wilt than g-C 3 N 4 nanosheets and CuONPs. CONCLUSION Thus, this study provides a new perspective on g-C 3 N 4 @CuO control of bacterial diseases in crops, and the mechanism is related to the destruction of cell membrane damage and motility disruption. © 2024 Society of Chemical Industry.
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Sustainability
·
2022
·Vol. 14
·期 24
·16348
·
IF 3.889
Qing-Bo Tu,Hui-Cong Shi,Ping Li,Sheng Sheng,Fu-An Wu
Ralstonia solanacearumis one of the ten most harmful plant bacteria worldwide, and traditional agrochemicals are not very effective in controlling this pathogen. Moreover, excessive pesticides always bring organic residues and resistant strains, which cause the unsustainability of the environment. In this paper, ferulic acid and essential oils are used as antibacterial materials. These compounds are natural substances with low toxicity and environmental safety. Through the structural optimization and the analysis of binary combined bacteriostatic efficiency, the MIC values of chlorobutyl ferulate (2e) and peppermint essential oil (EO1) were 0.64 mg/mL and 2.02 mg/mL, respectively, and the MIC value of2e-EO1(mass ratio 1:1.5) was 0.40 mg/mL. The growth rate of bacteria treated with2e-EO1was inhibited, the OD590nmvalue of cell membrane decreased by 57.83%, and the expression levels ofhrpB,pehC,pilT,polA,aceE,egl, andphcAwere downregulated to 18.81%, 30.50%, 14.00%, 44.90%, 86.79%, 23.90%, and 27.56%, respectively. The results showed that2e-EO1had a synergistic inhibitory effect againstR. solanacearum. It significantly affected the formation of the bacterial cell membrane and the expression of pathogenic genes. Consequently,2e-EO1provides the potential to become a green pesticide and can promote the sustainability of the agricultural ecological environment.Keywords:Ralstonia solanacearum;ferulic acid;essential oil;antibacterial activity;synergistic effect;pathogenic gene
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Plant Methods
·
2022
·Vol. 18
·期 1
·1-11
·
IF 5.827
Xu Cuihong,Zhong Lingkun,Huang Zeming,Li Chenying,Lian Jiazhang,Zheng Xuefang,Liang Yan
Background Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis-R. solanacearum system. However, the progress of R. solanacearum infection in Arabidopsis is still unclear.Results We generated a bioluminescent R. solanacearum by expressing plasmid-based luxCDABE. Expression of luxCDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 104 to 108 CFU·mL−1. After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants.Conclusion sBioluminescent R. solanacearum strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of R. solanacearum-Arabidopsis interaction in the future.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yao Wang,Liang Yang,Xiao Zhou,Ye Wang,Yijia Liang,Binshao Luo,Yuhao Dai,Zhouling Wei,Shili Li,Rong He,Wei Ding
The exploration of biopolymer-based materials to avoid hazardous chemicals in agriculture has gained enormous importance for sustainable crop protection. Due to its good biocompatibility and water solubility, carboxymethyl chitosan (CMCS) has been widely applied as a pesticide carrier biomaterial. However, the mechanism by which carboxymethyl chitosan-grafted natural product nanoparticles induce tobacco systemic resistance against bacterial wilt remains largely unknown. In this study, water-soluble CMCS-grafted daphnetin (DA) nanoparticles ( [email protected] ) were successfully synthesized, characterized, and assessed for the first time. The grafting rate of DA in CMCS was 10.05 %, and the water solubility was increased. In addition, [email protected] significantly increased the activities of CAT, PPO and SOD defense enzymes, activated the expression of PR1 and NPR1 , and suppressed the expression of JAZ3 . [email protected] could induce immune responses against R. solanacearum in tobacco, including increases in defense enzymes and overexpression of pathogenesis-related (PR) proteins. The application of [email protected] effectively suppressed the development of tobacco bacterial wilt in pot experiments, and the control efficiency was as high as 74.23 %, 67.80 %, 61.67 % at 8, 10, and 12 days after inoculation. Additionally, [email protected] has excellent biosafety. Therefore, this study highlighted the application of [email protected] in manipulating tobacco to generate defense responses against R. solanacearum , which can be attributed to systemic resistance.
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Chemical and Biological Technologies in Agriculture
·
2024
·Vol. 11
·期 1
·1-17
·
IF 5.2
Zhang Hongbao,Cai Liuti,Yuan Kai,Liu Zhongwei,Ran Maoyang,Chen Siang,Cai Wu,Rao Cheng,Cai Lin,Zhou Dong
Background As a vital soil-borne pathogenic bacterium, Ralstonia solanacearum can cause wilt disease in multiple Solanaceae plants. Several phages, such as ϕPB2, could infect R . solanacearum acting as a potential biological control agent in soil. In addition, some nanoparticles, especially copper preparation, also showed high toxicity on R. solanacearum with low toxicity on plant. However, whether they can be administered in combination and how effective they are in inhibiting the plant disease caused by R . solanacearum is known very little. Results In this work, the characterization of CuONPs using scanning electron microscope, transmission electron microscope, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction ascertained the presence of CuONPs which were nanometer particle of 83 nm. Then it was found that combined application of CuONPs with phage (ϕPB2) was superior to that of ϕPB2 or CuONPs alone in controlling tobacco bacterial wilt, with the CuONPs (250 mg/mL) and phage (10 6 PFU/mL) ratio being the best, at 79.1%. The combination of CuONPs and ϕPB2 also showed no obvious toxicity on tobacco growth than control like single application of CuONPs or ϕPB2. Furthermore, the transcriptome changes of R . solanacearum analysis indicated that the combination application and single allocation of CuONPs could inhibit “biofilm formation”, molecular function, biological processes, cellular components, metabolic process, and so on. In addition, the combination application showed higher inhibition of motility and biofilm, and better enhancement of cell membrane permeability, protein leakage, MDA concentration, and enzyme activity of their respiratory chain dehydrogenase than single application of CuONPs or phage (ϕPB2). Transcriptomes analysis also supported that the addition of ϕPB2 enhanced the toxicity of CuONPs by influencing the ABC transporters and quorum sensing, metabolic processes, and cellular biosynthetic processes of R. solanacearum . Conclusion In total, our work not only proposed a novel way to increase the bactericidal effect of nanomaterials by adding phage, but also discovered the influence, synergistic effects, and mechanisms, which is useful to design novel way to combat phytopathogenic bacteria in the complicated environment. Graphical abstract
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MICROBIOLOGICAL RESEARCH
·
2016
·
IF 2.723
Waseem Raza,Ning Ling,Dongyang Liu,Zhong Wei,Qiwei Huang,Qirong Shen
The volatile organic compounds (VOCs) produced by soil microbes have a significant role in the control of plant diseases and plant growth promotion. In this study, we examined the effect of VOCs produced by Pseudomonas fluorescens strain WR-1 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum . The VOCs produced by P. fluorescens WR-1 exhibited concentration dependent bacteriostatic effect on the growth of R. solanacearum on agar medium and in infested soil. The VOCs of P. fluorescens WR-1 also significantly inhibited the virulence traits of R. solanacearum . The proteomics analysis showed that the VOCs of P. fluorescens WR-1 downregulated cellular proteins of R. solanacearum related to the antioxidant activity, virulence, inclusion body proteins, carbohydrate and amino acid synthesis and metabolism, protein folding and translation, methylation and energy transfer, while the proteins involved in the ABC transporter system, detoxification of aldehydes and ketones, protein folding and translation were upregulated. This study revealed the significance of VOCs of P. fluorescens WR-1 to control the tomato wilt pathogen R. solanacearum . Investigation of the modes of action of biocontrol agents is important to better comprehend the interactions mediated by VOCs in nature to design better control strategies for plant pathogens.
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Advanced Agrochem
·
2026
·
IF 9.2
Cuiyun Zhou,Qinghong Zeng,Chang Tang,Dan Sun,Zixuan Li,Shuilan Zhang,Tao Zheng,Zhaoyang Zhang,Mengke Wang,Shun He,Wa Gao
Nanomaterials offer new opportunities for sustainable control of crop diseases caused by soil-borne pathogens. Here, cobalt-encapsulated nitrogen-doped carbon nanotubes (Co@N-CNTs) with tubular and bamboo-like morphologies were synthesized and evaluated against tomato bacterial wilt. Co@N-CNTs showed strong antibacterial activity against Ralstonia solanacearum in vitro , with the tubular morphology being more effective. Mechanistic analyses indicated that Co@N-CNTs induced reactive oxygen species-mediated oxidative damage in R. solanacearum , resulting in DNA damage, cytoplasmic leakage, and membrane disruption. In pot experiments, Co@N-CNTs reduced disease severity and delayed progression, with efficacy comparable to Zhongshengmycin. Transcriptomic and biochemical analyses showed tissue-specific regulation of plant defense, reducing infection-induced SA accumulation in stems while enhancing the expression of several defense-related genes in leaves, whereas JA contents were not significantly altered. Co@N-CNTs showed no detectable adverse effects on tomato growth and exhibited low acute toxicity to Italian honeybees. Collectively, these findings demonstrate that Co@N-CNTs can control soil-borne bacterial diseases and hold promise for sustainable agriculture.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2026
·
IF 4.8
Yao Wang,Yonghao Liu,Lei Yang,Zichen Li,Mengxiao Xie,Deping Ke,Gui Gao,Zhijun Chen,Jiaxi Yuan,Hong Zhou,Shili Li,Wei Ding
Plant-derived trans- anethole (TAN) exhibits favorable antibacterial activity and biocompatibility, but poor solubility, photolysis, and volatility limit its use. Here, we prepared a TAN nanoemulsion (TAN-NE) via low-energy emulsification using non-ionic surfactants (polyethylene glycol tert -octylphenyl ether and polyoxyethylenated castor oil) and a co-surfactant (anhydrous ethanol). TAN-NE had spherical morphology, particle size of 20.68 nm, formulation yield (92.16%), encapsulation efficiency (88.49%), and loading efficiency (3.60%). It showed improved water solubility, photostability, and structural stability under various pH, ionic strength, temperature, and storage conditions. In vitro experiments demonstrated that TAN-NE enhanced the antibacterial efficacy of TAN against Ralstonia solanacearum by inhibiting protein and EPS synthesis, damaging cell integrity, reducing motility and biofilm formation, and the downregulating virulence genes. More importantly, TAN-NE displayed an efficient protective activity of 71.74% against tobacco bacterial wilt on 15-day post inoculation, surpassing those of TAN (52.17%) and thiodiazole copper (42.39%). Additionally, TAN-NE also exhibited good biosafety to earthworms, seeds, tobacco plants and soil environment. Collectively, our study provides a guidance for the nanoemulsion system of insoluble natural product.
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BMC PLANT BIOLOGY
·
2026
·
IF 5.6
Yang Ping,Yang Qian,Chang Yingying,Li Jihua,Chen Rupei,Gelaye Yohannes,Liu Nian,Huang Li,Zhou Xiaojing,Chen Weigang,Yu Bolun,Ding Yingbin,Jiang Huifang,Liao Boshou,Lei Yong,Luo Huaiyong
Peanut ( Arachis hypogaea L.) is an important oil and economic crop, and its production has long been severely threatened by soil-borne bacterial wilt (BW) disease. However, the molecular mechanism of host resistance to it has not yet been systematically elucidated. In this study, the highly resistant peanut variety Zhonghua 6 was used as the research object. Through transcriptomic analysis, a total of 1,122 differentially expressed genes (DEGs) were identified between carefully designed treatment and control groups. WGCNA analysis led to the discovery of 14 hub genes, including two cytochrome P450 genes and a UGDH gene. Through metabolomic analysis, 1,614 differentially accumulated metabolites (DAMs) were identified, and 6-methylcoumarin, erucamide, and piceatannol were confirmed to inhibit the growth of R. solanacearum. Integrative transcriptomic and metabolomic analyses uncovered a comprehensive immune regulatory network consisted of genes involved in key pathways associated with R. solanacearum infection such as MAPK signaling, plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis, and ABC transporter. Overall, these results provide new insights into the molecular mechanisms governing peanut resistance to R. solanacearum , which might assist in the mining of resistance-related genes, developing of new disease control measures as well as breeding of novel disease-resistant cultivars in peanut.
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危险品化学品经营许可证（带存储）