COLLOIDS AND SURFACES B-BIOINTERFACES
·
2011
·
IF 2.78
Haiyan Wang,Dongyan Sun,Zhian Tan,Wu Gong,Lun Wang
In this work, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of α-fetoprotein (AFP) was fabricated using Ru(bpy) 3 2+ -encapsulated liposome as the label and electrodeposited gold nanoparticles (GNPs) as the immobilizing support. Great signal amplification was achieved since liposome could encapsulate large amount of reporter molecules and GNPs could provide large active surface. Under optimized conditions, with sandwich type format, a linear range of AFP from 0.005 to 0.2 pg/mL and an extremely low detection limit of 0.001 pg/mL was obtained, much lower than that in previous reports. The proposed ECL immnuosensor showed high sensitivity, specificity, and good stability, which may open a new door to ultrasensitive detection of proteins in clinical analysis.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Jiang Yilin,Xi Ruonan,Yin Jinlin,Sun Chen,Li Yukong,Wu Chao,Zhang Chi,Fei Honghan
Three-dimensional lead halide hybrids exhibit excellent photophysical properties but suffer from inherent instability. In contrast, two-dimensional layered lead halides offer enhanced environmental stability, yet their strongly bound excitons restrict efficient charge transport. Here we present a covalent intercalation strategy involving the benchmark photosensitizer [Ru(bpy) 3 ] 2+ into a layered lead halide framework, featuring cationic [Pb 23 X 42 ] 4+ (X − = Cl − or Br − ) layers pillared by [Ru(bpy) 3 ] 2+ ligands via Pb 2+ -carboxylate coordination. This hybrid material achieves nearly full visible-light absorption and efficient photoinduced charge transfer from [Ru(bpy) 3 ] 2+ to the lead halide layers. This affords efficient CO 2 -to-CO photoreduction with an apparent quantum efficiency of ~3.0% at 500 nm, exceeding the performance of all previously reported organolead halide photocatalysts. Mechanistic studies indicate that the [Ru(bpy) 3 ] 2+ ligands enhance charge transport to Pb 2+ sites, facilitating CO 2 activation and reducing the reaction barrier for the *COOH intermediate. This work establishes a paradigm for intercalation chemistry in robust layered lead halide hybrids.
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RSC Advances
·
2016
·Vol. 6
·期 57
·51591-51597
·
IF 3.289
Dongdong Lu,Fangyuan Gai,Zhen-An Qiao,Xue Wang,Tao Wang,Yunling Liu,Qisheng Huo
This work demonstrated that a series of ultrabright fluorescent silica nanoparticles have been synthesized and designed via the reverse microemulsion method to encapsulate (2,2′-bipyridine)2-(5-aminophenanthroline) Ru bis(hexafluorophosphate) (Rubpy-phen). Transmission electron microscopy (TEM), scanning electron microscopy (SEM), dynamic light scattering (DLS), inductively coupled plasma (ICP) and fluorescence spectra were used to study the morphology and properties of the nanoparticles. By introducing ethanol into the reaction system, the obtained uniform fluorescent nanoparticles showed monodispersity and controllable size. With an increase of nanoparticle size (from 50–180 nm), the relative brightness of the nanoparticles was greatly improved from 534 to 86 972 by comparison with free dye molecules. Due to the protection of the silica matrix, the fluorescence of high concentration dye-doped silica nanoparticles (Rubpy-phen NPs) is not quenched, which results in higher fluorescence intensity and relative brightness. Moreover, the silica matrix can also protect Rubpy-phen from leakage. Due to the high brightness and biocompatibility, Rubpy-phen NPs have potential applications in the biological field.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Nian Lu,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen
In this paper, a PSA/Ru(bpy) 3 2+ modified electrode was prepared by electrochemical polymerization and used as the working electrode in ECL detection of melamine. Under the optimal conditions, the sensor shows a wide linear range of 1.0×10 -7 ~1.0×10 -5 mol/L, detection limit of 2.5×10 -8 mol/L with good reproducibility and stability. The method is expected to be a new method for the detection of melamine, which is simple and convenient.
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TALANTA
·
2023
·
IF 6.1
Kai Zheng,Jiangfei Pan,Zipei Yu,Changqing Yi,Mei-Jin Li
We have proposed a signal dual-amplification electrochemiluminescence (ECL) strategy based on tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) as chromophores confined with three-dimensional (3D) zinc oxalate metal−organic frameworks (Ru(bpy) 3 2+ @MOFs) for the detection of miRNA-21. The three-dimensional chromophore connectivity in zinc oxalate MOFs provided a network among Ru(bpy) 3 2+ units, shielding the chromophores from solvent molecules and resulting in high Ru(II) complex emission efficiency. Additionally, we discovered that magnetic beads (MBs) used as carrier for enriched signals contribute to enhanced ECL intensity of the chromophore. To evaluate its clinical application, we applied this method to determine the concentration of miRNA-21 solutions ranging from 1.56 to 100 nM, obtaining a calibration curve of ECL intensity versus logarithm of concentration (logC) of miRNA-21 with a high correlation coefficient. This work demonstrates the construction of a signal amplification strategy ECL biosensor for miRNA using Ru(bpy) 3 2+ @MOF systems and its application in ECL detection for analyte methodology.
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ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 6
·639-644
·
IF 1.618
Shan Xueling,Pan Yuting,Chen Xiaohui,Wang Wenchang,Chen Zhidong
A novel electrochemiluminescence (ECL) sensor for detection of brilliant blue FCF (BB) has been developed using Ru@ Zn-MOF/nafion modified GCE (glass carbon electrode) in this research. Different from conventional method for usage of Ru(bpy) 3 2+ in solution-phase, Ru(bpy) 3 2+ here was immobilized on a zinc-metal-organic-framework (Zn-MOF). After adding BB, a significant quenching phenomenon of ECL intensity was observed. The behavior of BB on the quenching effect of Ru(bpy) 3 2+ /Zn-MOF in different conditions was investigated thoroughly and the detection limit was achieved to 2.5 × 10 −8 M in an optimized condition. Furthermore, the interference of some conventional ions and amino acids to the detection of BB was also investigated. Additionally, the composite showed a good effect on the detection of BB in commercial samples. The proposed sensor provided a promising platform for food safety analysis, environmental monitoring and clinical testing.
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NANOTECHNOLOGY
·
2018
·Vol. 30
·期 2
·025501
·
IF 3.404
Danqing Liu,Tianchan Li,Weicheng Huang,Zhuang Ma,Wei Zhang,Rui Zhang,Hong Yan,Bin Yang,Shaoqin Liu
Foodborne pathogens are perpetual threats to human and animal health. Detection of pathogens requires accurate, sensitive, rapid and point-of-care diagnostic assays. In this study, we described a simple and sensitive electrochemiluminescent (ECL) assay to detect the deadly bacteria Escherichia coli O157:H7 by {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-coated ZnO nanorods arrays (NAs). The {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-coated ZnO NAs were fabricated by immobilizing {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+} on ZnO NAs with a large specific surface area and good conductivity. An {\rm{Ru}}{{({\rm{bpy}})}_{3}}^{2+}-2-(dibutylamino)-ethanol (DBAE) system coated on ZnO NAs exhibits high ECL intensity, rapid response and good stability. This system was further developed as an ECL immunosensor used in the detection of E. coli O157:H7. The proposed ECL immunosensor exhibits a broad detection range within the scope of 200–100 000 CFU ml−1 and quite a low detection limit of 143 CFU ml−1. The high specificity, remarkable reproducibility and good stability offer a sensitive, selective, and convenient pathway for detecting E. coli O157:H7 in the field of food safety and clinical diagnosis.
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Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 7
·1361-1367
·
IF 4.872
Juanjuan Li,Xiaochun Hu,Shuo Shi,Yiwei Zhang,Tianming Yao
Early disease diagnosis is very important for the prevention or mitigation of metastasis. Effective and efficient methods are needed to improve the diagnosis and assessment of diseases. Thrombin is a biomarker for diagnosis of some diseases, such as pulmonary metastases and diseases associated with coagulation abnormalities. Lots of methods for detecting thrombin have been reported, however, most of them were based on a single aptamer and needed modification of aptamers, and label free sensors for thrombin detection based on an aptamer pair are challenging. In this work, we present three label free sensing platforms based on [Ru(bpy)2(o-mopip)]2+ (bpy = 2,2-bipyridine; o-mopip = 2-(2-methoxylphenyl)imidazo[4,5-f][1,10]phenanthroline) (OMO) and graphene oxide (GO) for thrombin detection. Thrombin detection using our three label free sensors is accomplished by specific target recognition based on aptamer pair TBA1/TBA2–thrombin, single aptamer TBA1–thrombin or TBA2–thrombin, respectively. Direct readouts of the target recognition is achieved by restoration of the fluorescence of OMO prequenched by GO. All of the three sensing platforms exhibited high sensitivity and selectivity. Furthermore, all sensing platforms were successfully applied to thrombin analysis in diluted bovine serum. According to the thrombin dependent response of the three platforms, the sensing platform based on aptamer pair TBA1 and TBA2 showed the highest sensitivity, widest linearity, best selectivity and recovery in diluted serum to thrombin. These results show that the sensing platform based on an aptamer pair have great potential for clinical diagnosis of disease-related biomarkers.
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CHINESE SCIENCE BULLETIN
·
2013
·Vol. 59
·期 2
·147-153
·
IF 1.319
Wang Wei,Yan Xiao Li,Zhan Lei,Leng Fei,Yang Xiao Xi,Huang Cheng Zhi
A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris(2,2′-bipyridyl)ruthenium(II)@SiO 2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)
2+ 3
) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru(bpy)
2+ 3
@SiO 2 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt-Ru(bpy)
2+ 3
@SiO 2 NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.
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TALANTA
·
2012
·
IF 3.794
Baomei Huang,Xibin Zhou,Zhonghua Xue,Guofan Wu,Jie Du,Di Luo,Tao Liu,Jing Ru,Xiaoquan Lu
Efficient and stable quenching of electrochemiluminescence of Ru(bpy) 3 2+ /TPA by malachite green(MG) and crystal violet(CV) at the glass carbon (GC) electrode is reported. A novel quenching mechanism has been proposed. Resonance energy transfer from the excited-state luminophore Ru(bpy) 3 2+⁎ to MG/CV and dynamic quenching are suggested as the mechanism for quenching ECL. The quenching mechanism is discussed in detail based on UV–visible absorption spectra, cyclic voltammograms, ECL curves and fluorescence methods . MG shows more efficient quenching than CV. Moreover, the quenched ECL intensity versus the concentration of MG and CV are linear over the concentration ranges of 8×10 −10 –8×10 −7 M and 3.46×10 −9 –5.5×10 −7 M, respectively. The corresponding limit of detection (LOD) was 1.0×10 −10 M for MG and 1.1×10 −10 M for CV ( S / N =3).
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Ziqi Wang,Yahui Ji,Nana You,Xiaoping Hu,Fangxin Du,Gen Liu
Testing ribavirin (RBV) is essential to promote its appropriate clinical use, mitigate resistance development, reduce adverse effects, and safeguard public health. Electrochemiluminescence (ECL) outperforms conventional methods in RBV detection, offering superior sensitivity and reduced background noise. In this work, ECL micro-reactors were prepared by incorporating Ru(bpy) 3 2+ into a mesoporous silica matrix with two-dimensional hexagonal structure (SBA-15). In this configuration, tripropylamine (TPA) was employed as a co-reactant, which interacted with Ru(bpy) 3 2+ to enhance the ECL signal. The quenching effect of ribavirin (RBV) on the Ru(bpy) 3 2+ -TPA system was confirmed through its significant suppression of ECL emission. A glassy carbon electrode (GCE) modified with SBA-15 loaded with Ru(bpy) 3 2+ (SBA-15@Ru) was fabricated as a novel ECL sensor. This sensor enabled the detection of RBV over a wide concentration range 1.0 × 10 −9 mol·L −1 to 1.0 × 10 −5 mol·L −1 , achieving a detection limit (LOD) of 3.33 × 10 −10 mol·L −1 . Additionally, the sensor exhibited outstanding stability and selectivity and was successfully applied for accurate quantification of RBV in pharmaceutical samples.
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RSC Advances
·
2022
·Vol. 12
·期 5
·3157-3164
·
IF 4.036
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
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