International Journal of Electrochemical Science
·
2023
·
IF 1.5
Liang Bai
Salbutamol, clenbuterol, ractopamine and albuterol have the effect of promoting protein synthesis and increasing muscle mass. Despite that these substances are banned or restricted in animal husbandry in many countries, some farmers still use them illegally to improve profits. Athletes can test positive for stimulants after ingesting food with these drug residues. In this study, the electrochemical behavior of salbutamol, clenbuterol, ractopamine and albuterol were investigated with carbon nanotubes modified glassy carbon electrodes. The linear detection range and detection limit of four stimulants were studied under optimal conditions.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2014
·
IF 2.829
Yajing Lei,Xiaoyi Li,Muhammad Sajid Hamid Akash,Lifang Zhou,Xiaojing Tang,Weixing Shi,Zhiming Liu,Shuqing Chen
Salbutamol (SAL) is known as quick-acting β-2 receptor agonist and its use in humans for pulmonary diseases and/or in animal feed is limited because of associated potential hazardous effects on health. Several techniques are available for the detection of SAL, but are expensive and time consuming. Here for the first time, a novel pre-assembled DNA immunoprobe-based immuno-PCR assay was developed to investigate the levels of SAL in human urine samples and compared the proposed immuno-PCR assay for the detection of SAL with that of direct competitive ELISA. Under the optimized conditions, the assay showed a linear range over seven orders of magnitude, whereas the limit of detection of SAL in buffer was found to be 21 fg/mL. Current immuno-PCR assay exhibited a 300-fold better detection limit for SAL as compared to one achieved with direct competitive ELISA using the same antibody. In conclusion, it has been found that the developed method is highly sensitive and simple method that can significantly enhance the detection sensitivity for small molecules. Moreover it can be modified and used for detecting other small molecules and/or chemicals that exist in the environment and are responsible for the environmental pollution.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Chengyu Duan,Jianyu Wang,Qikai Liu,Yi Zhou,Yanbo Zhou
Salbutamol (SAL) and Atenolol (ATL), two common medical ingredients acting on adrenal receptor, are often used for treating chronic illnesses. However, the incomplete metabolism in organism and widespread utilization lead to unneglected problems of water pollution, which the traditional treatment plant cannot satisfactorily deal with. For efficient removal of SAL and ATL, a kind of silanized β-cyclodextrin compound adsorbents (GD-EDTS) was designed based on the ionization features of two pollutants. Through an introduction of rich carboxyl groups by N-[(3-Trimethoxysilyl)propyl]ethylenediamine triaceticacid trisodium salt (EDTS), GD-EDTS surface obtains an appropriate electronegativity to meet different forms of IOCs and maintain a good adsorption performance in a wide pH range. The maximum adsorption capacities of SAL and ATL are 140.24 mg/g and 236.92 mg/g, respectively. And a remarkable rise is known from the adsorption capacity of SAL and ATL increasing to 162% and 706%, respectively, after EDTS modification. Adsorption data fit the Sips adsorption isotherms and the pseudo second-order kinetic model, and a weak competitive behavior between SAL and ATL indicates two pollutants can be removed simultaneously by GD-EDTS in their binary system. In this work, GD-EDTS is suggested to be an efficient adsorbent in the removal of SAL and ATL.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yanli Zhou,Huanqing Zhang,Zhu Chang,Baoxian Ye,Maotian Xu
A highly sensitive electrochemical sensor for the simultaneous determination of clenbuterol (CLB) and salbutamol (SAL) was fabricated by the modification of graphene-Nafion (GN-Nafion) composite film on a glassy carbon electrode (GCE). Both CLB and SAL caused a pair of redox peaks at the GN-Nafion/GCE in 0.04 M Britton-Robinson buffer solution. Obvious anodic peak currents for the oxidation of both CLB and SAL were observed under the optimal condition. The difference of oxidation peak potentials for CLB and SAL was about 200 mV, which made it possible for simultaneous determination of these compounds using the GN-Nafion/GCE. The detection limit for CLB and SAL was 0.13 μM and 0.11 μM (S/N=3), respectively. The proposed method was applied to simultaneously detect CLB and SAL in pork meat with satisfactory results. Otherwise, the excellent reproducibility, stability and selectivity were obtained at the proposed electrode.
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Analytical Methods
·
2014
·Vol. 6
·期 6
·1928-1935
·
IF 1.938
Yinfeng Li,Zhuo Ye,Peili Luo,Ying Li,Baoxian Ye
A novel voltammetric sensor for salbutamol is fabricated via self-assembly of gold nanoparticles on multi-walled carbon nanotubes and Nafion modified electrode. This sensor could improve the oxidation of salbutamol intensively. The oxidation process of salbutamol is studied and some dynamic parameters are calculated using various electrochemical techniques. Under the optimum conditions, the response peak currents have a linear relationship with the salbutamol concentrations in the range of 9.0 × 10−8 to 7.0 × 10−6 mol L−1 with a detectable limit of 5.0 × 10−8 mol L−1. The proposed method is successfully applied to the determination of salbutamol in commercial pharmaceuticals and human serum samples with good accuracy and satisfactory recovery.
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Food Bioscience
·
2026
·
IF 6.2
Tianzhen Yin,Qi Wang,Yankun Peng,Hao-Ran Song
AgNPs–CNP enabled NaOH-assisted SERS detection of salbutamol in pork. • AgNPs–CNP-10 gave the strongest response among the tested formulations. • Bands at 1603 and 1360 cm −1 supported preliminary dual-band calibration. • Response-based LOD and LOQ were 0.119 and 0.396 μg/g, respectively. • SAL/RAC mixtures showed composition-dependent spectral overlap. Salbutamol (SAL), a representative β-adrenergic agonist, poses potential risks to food safety when illegally present in animal-derived foods. In this study, silver nanoparticles prepared in the presence of carbon nanopowder (AgNPs–CNP) were examined for surface-enhanced Raman spectroscopy (SERS) measurement of SAL-containing extracts prepared from fortified pork. Among the tested preparations, AgNPs–CNP-10 produced the strongest SAL response, and a 0.9 mol/L NaOH stock solution produced the strongest response among the tested aggregation conditions. Across the recorded fortification series, the bands at 1603 and 1360 cm −1 showed level-dependent spectral changes. All droplets at a given recorded level originated from the same fortified and extracted pork portion, and the held-out droplets therefore described only droplet-level variation within the same extract series. Moreover, because the aliquot volume used in the original fortification step could not be reconstructed from the surviving records, the recorded level labels and derived regression statistics are not presented as traceable sample concentrations or method-validation parameters. An exploratory SAL/RAC coexistence experiment showed composition-dependent changes in adjacent spectral regions and progressively greater band overlap as the SAL:RAC ratio decreased. This work therefore provides a matrix-oriented exploratory description of the AgNPs–CNP/NaOH response. Verified fortification, independently prepared samples, recovery and trueness assessment, reference-method confirmation, broader selectivity testing, spatial and batch reproducibility, day-to-day evaluation, and storage-stability studies are required before quantitative or practical screening performance can be assessed. Download: Download high-res image (387KB) Download: Download full-size image
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yingshiyu Lin,Lingbo Liu,Gaozhi Ou,Wensheng Huang,Kangbing Wu
Salbutamol (SAL), a drug originally intended for the treatment of bronchial and pulmonary diseases, has repeatedly been used for doping in competitive sports. Herein, an integrated array (NFCNT array) that prepared by template-assisted scalable filtration using Nafion-coated single-walled carbon nanotube (SWCNT) is presented for the rapid field detection of SAL. Spectroscopic and microscopic measurements were used to confirm the introduction of Nafion onto the surface of the array and to analyze the resulting morphological changes. The effects of Nafion addition on the resistance and electrochemical properties of the arrays (e.g., the electrochemically active area, charge-transfer resistance, and adsorption charge) are also discussed in depth. With an electrolyte/Nafion/SWCNT interface and moderate resistance, the NFCNT-4 array prepared containing 0.04 wt% Nafion suspension exhibits the greatest voltammetric response to SAL. Subsequently, a possible mechanism for the oxidation of SAL was proposed, and a calibration curve in the range of 0.1–15 μM was established. Finally, the NFCNT-4 arrays were applied to the detection of SAL in human urine samples with satisfactory recoveries.
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Pharmaceutics
·
2020
·Vol. 12
·期 1
·35
·
IF 4.421
Rui Zhang,Jie Wu,Siyu Liu,LiangJun Deng,Junhua Hu,Xi Chen,Wen Tan
Recent studies have shown that β2-Adrenoreceptor is a regulator of the a-synuclein gene driving risk of Parkinson’s disease. The β2-AR agonist (R)-salbutamol, eutomer of rac-salbutamol, may hold therapeutic potential for Parkinson’s disease (PD) following nasal administration. In this study, we use desorption electrospray ionization mass spectrometry (DESI-MS) to analyze spatial distribution of (R)-salbutamol in rat brain following nasal and intravenous administration. Here, we report that (R)-salbutamol efficiently deliver to the brain and had more drug dosage exposure in rat’s brain through nasal route administration than that of intravenous route administration. In conclusion, administering (R)-salbutamol through nasal route of administration may hold advantages in improving spatial distribution and increased exposure of drug in brain.Keywords:Parkinson’s disease;β2-adrenergic receptor;salbutamol;nasal administration;desorption electrospray ionization
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RSC Advances
·
2018
·Vol. 8
·期 10
·5536-5541
·
IF 2.936
Shihua Yu,Zhigang Liu,Jianpo Zhang,Hongwei Li,Na Xu,Xin-xin Yuan,Yuqing Wu
To date, great achievements with GC-MS, HPLC-MS, and fluorescence biosensing techniques have been made to detect illegal additives of salbutamol (SAL) in swine meat. However, these methods are not suitable for rapid on-site screening due to either costly instruments or rather complicated and/or time consuming sample pretreatments. Herein, a simple, rapid and ultrasensitive approach based on an azo-coupling reaction and surface-enhanced resonance Raman scattering (SERRS) is presented. By combining with a magnetic SERS substrate, an indirect detection for SAL, with a LOD of 1.0 × 10−11 M (2.39 pg mL−1), was realized. Moreover, a colorimetric method for naked eye detection was successfully carried out for rapid screening of SAL in concentrations higher than 2.09 × 10−5 M (5 μg mL−1). In addition, the proposed method was successfully applied for the rapid determination of SAL in real swine meat. The entire process, including pretreatment, coupling reaction and SERRS detection, was performed within 7 min. Moreover, the SERRS fingerprint band being specific to corresponding functional group guarantees the selectivity for the target molecule. Therefore, the proposed strategy in the present study offers a new way to identify trace amounts of analytes, such as SAL as well as other illegal additives in health-related products and food.
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Analytical Methods
·
2014
·Vol. 6
·期 12
·4228-4233
·
IF 1.938
Liqiang Liu,Huang Kuang,Chifang Peng,Libing Wang,Chuanlai Xu
Salbutamol (SAL) is a short-acting β2-adrenergic receptor agonist (β-agonist) forbidden in livestock production. To develop a more sensitive and specific detection method for SAL, a new hapten was synthesized, keeping the structure of benzene ring side of SAL while changing the tert-butyl group structure that SAL shares with other β-agonists. Ten mice were immunized with a SAL hapten–keyhole limpet hemocyanin (KLH) conjugate, and cells were selected within a urine sample at the cell fusion stage. The best monoclonal antibody (Mab) had an IC50 value of 0.31 ng mL−1 in PBST buffer and an IC50 value of 0.19 ng mL−1 in a swine urine sample diluted five times (with a lower B0 value), and had no cross-reactivity with clenbuterol and other β-agonists. These results show that the hapten design was successful and that the Mab can potentially be used in highly sensitive immunoassays for specifically monitoring the illegal use of SAL in real samples.
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Analytical Methods
·
2015
·Vol. 7
·期 13
·5635-5640
·
IF 1.821
Yajing Lei,Xiaoyan Yao,Dai Zhang,Lifang Zhou,Weixian Lu,Shuqing Chen
Salbutamol (SAL) is used to promote animal growth, because it increases feeding efficiency and carcass lean percentage. SAL has been banned in many countries because of the potential hazard to human health. To investigate the SAL residue level in humans, a sensitive direct competitive ELISA was developed. Under the optimal experimental conditions, the limit of detection (LOD) for the assay was 0.01 ng mL−1, and the standard curve was constructed at concentrations of 0.01–100 ng mL−1. We used the developed assay to detect the urinary concentration of SAL in 1648 participants. SAL was detected in 96% of participants with a concentration ranging from the LOD to 30.83 ng mL−1. Pregnant women were found to have a lower SAL concentration than non-pregnant women. Sources of exposure, risk assessment and measures for reducing the exposure level require further investigation.
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ANALYTICAL SCIENCES
·
2021
·Vol. 37
·期 9
·1289-1294
·
IF 2.081
Wenjie LI,Chenchen WANG,Huidong LI,Zilei CHEN,Mengmeng YAN
The formation of self-assembled monolayers (SAMs) is a normal method for the immobilization of biorecognition elements immobilized on SPR sensors. With this method, mercaptopropionic acid (MPA) with carboxylic and thiol group is the most commonly used. Dimercaptosuccinic acid (DMSA) having two carboxylics and two thiol groups is a classical antidote for heavy metal ions. In this paper, DMSA was first used to form SAMs to connect the antigen on the chip of a surface plasmon resonance (SPR) immunosensor for detection of salbutamol (SAL), and the results were compared with a traditional (MPA)-SPR sensor. Dihydrolipoic acid(DHLA)-SPR showed that the recognition efficiency of antigen and antibody of DMSA-SPR immunosensor was 170.1% at room temperature with the linear range of 5 – 150 ng/mL. The recovery rate of this sensor applied to SAL detection in pork reached 94.9 – 108.0% and the limit of quantification (LOQ) was 5 ng/mL. The results were in good correlation with the analysis results of ultra-high phase liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis. This novel DMSA-SPR immunosensor provides insight into a new idea and method for improving the sensitivity of SPR immunosensors and can be widely used in the detection of other small molecules.
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