Nature Communications
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2020
·Vol. 11
·期 1
·1-11
·
IF 12.121
Yang Chenhui,Aslan Hüsnü,Zhang Peng,Zhu Shoujun,Xiao Yong,Chen Lixiang,Khan Nasar,Boesen Thomas,Wang Yuanlin,Liu Yang,Wang Lei,Sun Ye,Feng Yujie,Besenbacher Flemming,Zhao Feng,Yu Miao
Bioelectricity generation, by Shewanella oneidensis ( S. oneidensis ) MR-1, has become particularly alluring, thanks to its extraordinary prospects for energy production, pollution treatment, and biosynthesis. Attempts to improve its technological output by modification of S. oneidensis MR-1 remains complicated, expensive and inefficient. Herein, we report on the augmentation of S. oneidensis MR-1 with carbon dots (CDs). The CDs-fed cells show accelerated extracellular electron transfer and metabolic rate, with increased intracellular charge, higher adenosine triphosphate level, quicker substrate consumption and more abundant extracellular secretion. Meanwhile, the CDs promote cellular adhesion, electronegativity, and biofilm formation. In bioelectrical systems the CDs-fed cells increase the maximum current value, 7.34 fold, and power output, 6.46 fold. The enhancement efficacy is found to be strongly dependent on the surface charge of the CDs. This work demonstrates a simple, cost-effective and efficient route to improve bioelectricity generation of S. oneidensis MR-1, holding promise in all relevant technologies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Mingxia Jiang,Yuyin Lin,Liyuan Xu,Zhihang Chen,Junxian Wu,Yong Yuan,Lihua Zhou
Extracellular respiratory bacteria (ERB) assume a significant role in biological and environmental remediation, so the rapid detection of ERB is essential to monitor its biological repair process. This study developed a microfluidic colorimetric biosensor for rapid and sensitive detection of ERB model strain Shewanella oneidensis MR-1. In the process of separation and enrichment of bacteria based on immune magnetic nanoparticles (MNPs), the biological signal is significantly amplified by the excellent peroxidase activity of flower-like CoOOH (FL-CoOOH). Firstly, immune MNPs, samples and immune FL-CoOOH were mixed and incubated within the microfluidic chip’s channel. Magnetic capture in the separation chamber yielded MNP-bacteria-CoOOH complex. Then, 3, 3′, 5, 5′-tetramethylbenzidine (TMB) substrate was added to make the FL-CoOOH nanozyme catalyze the formation of yellow product. Finally, the target bacteria in the microplate were quantitatively detected. Based on the excellent simulated enzyme activity and stability of FL-CoOOH, the detection can be completed within 45 min after the introduction of the microfluidic chip. The detection was 5×10 1 ∼5×10 6 CFU/mL. Compared with the previously reported colorimetric sensors for MR-1, the sensitivity was increased by 99.54 % to 23 CFU/mL. This study uniquely integrates optical sensors and microfluidic technology for rapid ERB detection with enhanced portability. The design of biosensor can facilitate further investigations into extracellular respiration mechanisms and rapid ecological environment restoration applications.
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BIODEGRADATION
·
2018
·Vol. 29
·期 2
·129-140
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IF 2.41
Mao Fei,Liu Xiaohong,Wu Kang,Zhou Chen,Si Youbin
Because of extensive sulfonamides application in aquaculture and animal husbandry and the consequent increase in sulfonamides discharged into the environment, strategies to remediate sulfonamide-contaminated environments are essential. In this study, the resistance of Shewanella oneidensis MR-1 and Shewanella sp. strain MR-4 to the sulfonamides sulfapyridine (SPY) and sulfamethoxazole (SMX) were determined, and sulfonamides degradation by these strains was assessed. Shewanella oneidensis MR-1 and Shewanella sp. strain MR-4 were resistant to SPY and SMX concentrations as high as 60 mg/L. After incubation for 5 days, 23.91 ± 1.80 and 23.43 ± 2.98% of SPY and 59.88 ± 1.23 and 63.89 ± 3.09% of SMX contained in the medium were degraded by S. oneidensis MR-1 and Shewanella sp. strain MR-4, respectively. The effects of the initial concentration of the sulfonamides and initial pH of the medium on biodegradation, and the degradation of different sulfonamides were assessed. The products were measured by LC–MS; with SPY as a substrate, 2-AP (2-aminopyridine) was the main stable metabolite, and with SMX as a substrate, 3A5MI (3-amino-5-methyl-isoxazole) was the main stable metabolite. The co-occurrence of 2-AP or 3A5MI and 4-aminobenzenesulfonic acid suggests that the initial step in the biodegradation of the two sulfonamides is S–N bond cleavage. These results suggest that S. oneidensis MR-1 and Shewanella sp. strain MR-4 are potential bacterial resources for biodegrading sulfonamides and therefore bioremediation of sulfonamide-polluted environments.
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2020
·
IF 7.864
Tongxu Liu,Xiaobo Luo,Yundang Wu,John R. Reinfelder,Xiu Yuan,Xiaomin Li,Dandan Chen,Fangbai Li
How metal-reducing bacteria transfer electrons during dissimilatory energy generation under electron acceptor-limited conditions is poorly understood. Here, we incubated the iron and manganese-reducing bacterium Shewanella oneidensis MR-1 without electron acceptors. Removal of soluble extracellular organic compounds (EOCs) dramatically retarded transfer of electrons to an experimental electron acceptor, Cr(VI), by MR-1. However, the return of either high MW (>3000 Da) or low MW (<3000 Da) soluble EOCs produced by MR-1 to washed cells restored Cr(VI) reduction though Cr(VI) reduction was fastest when both size fractions were added together. Spectral and electrochemical characterization of EOCs indicated the presence of flavins and c-type cytochromes (c-Cyts). A model of the kinetics of individual elementary reactions between cells, flavins, released c-Cyts, and Cr(VI), including the direct reduction of flavins, released c-Cyts, and Cr(VI) by cells and the indirect reduction of Cr(VI) by reduced forms of flavins and released c-Cyts, was developed. Model results suggest that released c-Cyts could act as electron mediators to accelerate electron transfer from cells to Cr(VI), and the relative contribution of this pathway was higher than that mediated by flavins. Hence, extracellular c-Cyts produced by MR-1 likely play a role in extracellular electron transfer under electron acceptor-limited conditions. These findings provide new insights into extracellular electron shuttling and the metabolic strategy of metal-reducing bacteria under electron acceptor-limited conditions.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Ling Xiong,Liying Ma,Meiqi Zhao,Haojian Zhang,Shaohua Chen
In present work, nanoscale zero-valent iron (nZVI) was successfully stabilized using fulvic acid (nZVI-FA). Shewanella oneidensis MR-1 (MR-1) was used to enhance the reactivity of the nZVI-FA. The results demonstrated that nZVI-FA combined with the MR-1 (nZVI-FA/MR-1) system could effectively solve the aggregation and surface passivation of nZVI, thereby significantly enhancing the reactivity of nZVI for Cr(VI) removal. The Cr(VI) removal efficiency of the nZVI-FA/MR-1 system (100 %) within 24 h was apparently higher than that of the ZVI-FA system (64.43 %). The enhancement of Cr(VI) removal is because fulvic acid (FA) FA can stabilize nZVI against aggregation; MR-1 could rapidly reduce the Fe(III) (hydr)oxides (FeHOs) passivation layers on the surface of nZVI-FA to biogenic Fe(II) [bio-Fe(II)], which in turn reduced Cr(VI). X-ray photoelectron spectroscopy (XPS) analysis revealed that the Fe(II)/Fe(III) ratio (1.20) and Cr(III)/Cr(VI) ratio (2.53) of the precipitates in the nZVI-FA/MR-1 system were much higher than that those in the nZVI-FA system (0.55 and 1.40, respectively). Three-dimensional excitation-emission matrix (3D-EEM) spectral analysis confirmed that tyrosine, tryptophan, and protein-like substances in extracellular polymeric substances (EPSs) from MR-1 are vital for the removal of Cr(VI). The mechanism analysis demonstrated that most Cr(VI) was reduced to Cr(III); Cr(III) existed as Cr 2 O 3 , FeCr 2 O 4 and Cr x Fe 1-x (OH) 3 precipitates, and some of the Cr(VI) was removed from the solution by adsorption and complexation. This study offers a low-cost, efficient, and environmentally friendly strategy to enhance the reactivity of nZVI for Cr(VI) removal, which has broad prospective applications.
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ENVIRONMENTAL RESEARCH
·
2020
·
IF 5.715
Zhen-Ni Yang,Ya-Nan Hou,Bo Zhang,Hao-Yi Cheng,Yang-Chun Yong,Wen-Zong Liu,Jing-Long Han,Shuang-Jiang Liu,Ai-Jie Wang
Biologically synthesized palladium nanoparticles (bio-Pd) have attracted considerable interest as promising green catalysts for environmental remediation. However, the mechanisms by which microorganisms produce bio-Pd remain unclear. In the present study, we investigated the roles of Shewanella oneidensis MR-1 and its NADH dehydrogenases and hydrogenases (HydA and HyaB) in bio-Pd production using formate as the electron donor. The roles of NADH dehydrogenases and hydrogenases were studied by inhibiting NADH dehydrogenases and using hydrogenase mutants (Δ hydA , Δ hyaB , and Δ hydA Δ hyaB ), respectively. The results showed ~97% reduction of palladium by S. oneidensis MR-1 after 24 h using 250 μM palladium and 500 μM formate. Electron microscopy images showed the presence of bio-Pd on both the outer and cytoplasmic membranes of S. oneidensis MR-1. However, the inhibition of NADH dehydrogenases in S. oneidensis MR-1 resulted in only ~61% reduction of palladium after 24 h, and bio-Pd were not found on the outer membrane. The mutants lacking one or two hydrogenases removed 91–96% of palladium ions after 24 h and showed more cytoplasmic bio-Pd but less periplasmic bio-Pd. To the best of our knowledge, this is the first study to demonstrate the role of NADH dehydrogenases of S. oneidensis MR-1 in the formation of bio-Pd on the outer membrane. It also demonstrates that the hydrogenases (especially HyaB) of S. oneidensis MR-1 contribute to the formation of bio-Pd in the periplasmic space. This study provides mechanistic insights into the production of biogenic metal nanoparticles towards their possible use in industrial and environmental applications.
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Environmental Science & Technology Letters
·
2017
·
IF 5.308
Jun-Cheng Han,Guo-Jun Chen,Li-Ping Qin,Yang Mu
Cr isotope fractionation during microbial reduction processes is commonly recognized as a promising tool in biogeochemistry and bioremediation. However, the mechanism of Cr isotope fractionation during microbial reduction is poorly understood. In this work, the relationship between the bacterial respiratory pathway and Cr isotope fractionation was investigated in Shewanella oneidensis MR-1. For comparison with the wild type, a mutant strain (ΔomcA/ΔmtrC) with a deficiency in extracellular Cr(VI) reduction was constructed by deleting the omcA and mtrC genes, which encode the terminal reductase during extracellular reduction. The magnitudes of Cr isotope fractionation (ε) for Cr(VI) reduction by the wild type and ΔomcA/ΔmtrC were −2.42 ± 0.68‰ and −2.70 ± 0.22‰, respectively. Surprisingly, the ε values were not significantly different between the two strains. This suggests that isotope fractionation is independent of the metal respiratory pathway during Cr(VI) reduction by S. oneidensis MR-1. Moreover, a three-step Cr isotope fractionation model that includes uptake, reduction, and efflux was proposed to exist during intracellular Cr(VI) reduction by S. oneidensis MR-1. The developed model provides a better understanding of Cr isotope fractionation during microbial reduction.
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Micro & Nano Letters
·
2020
·Vol. 15
·期 14
·1007-1011
·
IF 0.975
Xiaojie Song,Fan Yang,Xiufang Wang,Kehua Zhang
Biogenetic nanocomposites research provides valuable methods for the green synthesis of nanomaterials. As a dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1 is used to reduce Ag + to Ag nanoparticles (Ag NPs) (diameter about 10 nm) under anaerobic conditions, resulting in the in-situ formation of Ag NPs immobilised on TiO 2 nanotubes (TNTs) (Ag/TNTs nanocomposites). The loading amount of Ag nanocrystals on the TNT surface can be controlled easily through adjusting the AgNO 3 concentration and further influence visible-light absorption efficiency of Ag/TNTs nanocomposites by decreasing the Ag loading amount. Ag/TNTs nanocomposites show superior photocatalytic efficiency under simulated sunlight than single TiO 2 nanomaterials. Moreover, the photocatalytic capacity of Ag/TNTs nanocomposites synergistic by S. oneidensis MR-1 is further enhanced and the degradation ratio of methylene blue reaches 92.3% within 30 min which attributed to a synergistic effect.
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CHEMOSPHERE
·
2020
·
IF 5.778
Juping You,Yingying Deng,Han Chen,Jiexu Ye,Shihan Zhang,Jingkai Zhao
An exoelectrogens, Shewanella oneidensis MR-1 ( S. oneidensis MR-1), was supplied to a microbial fuel cell (MFC) to enhance the degradation of a recalcitrant organic compound, o -xylene. The experimental results revealed that, with the addition of the S. oneidensis MR-1, the o -xylene removal efficiency increased by 35–76% compared with the original MFC. The presence of the S. oneidensis MR-1 not only improved the activity of the biofilm in the bioanode but also developed the connections between the bacteria by nanowires . Therefore, the maximum power density increased from 52.1 to 92.5 mW/m 3 after the addition of the S. oneidensis MR-1. The microbial community analysis showed that adding the S. oneidensis MR-1 increased the biodiversity in bioanode. The dominant exoelectrogens shifted from Zoogloea sp., Delftia sp., Achromobacter sp., Acinetobacter sp., Chryseobacterium sp., and Stenotrophomonas sp. to Zoogloea sp., Delftia sp., Shewanella sp., Achromobacter sp., Hydrogenophaga sp., Sedimentibacter sp. and Chryseobacterium sp.. Furthermore, the cyclic voltammetry analysis showed that the outer membrane bound protein complex of OmcA–MtrCAB was involved as direct electron transfer pathway in the S. oneidensis MR-1 containing bioanode. We believed that this work is promising to provide optional strategy for efficient VOCs degradation by adjusting the microbial community in the bioanode.
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Journal of Environmental Sciences
·
2021
·
IF 5.565
Cholnam Ri,Jingchun Tang,Feng Liu,Honghong Lyu,Fengxiang Li
Biochar, carbonaceous material produced from biomass pyrolysis, has been demonstrated to have electron transfer property (associated with redox active groups and multi condensed aromatic moiety), and to be also involved in biogeochemical redox reactions. In this study, the enhanced removal of Cr(VI) by Shewanella oneidensis MR-1(MR-1) in the presence of biochars with different pyrolysis temperatures (300 to 800 °C) was investigated to understand how biochar interacts with Cr(VI) reducing bacteria under anaerobic condition. The promotion effects of biochar (as high as 1.07~1.47 fold) were discovered in this process, of which the synergistic effect of BMBC700(ball milled biochar) and BMBC800 with MR-1 was noticeable, in contrast, the synergistic effect of BMBCs (300–600 °C) with MR-1 was not recognized. The more enhanced removal effect was observed with the increase of BMBC dosage for BMBC700+MR-1 group. The conductivity and conjugated O-containing functional groups of BMBC700 particles themselves has been proposed to become a dominant factor for the synergistic action with this strain. And, the smallest negative Zeta potential of BMBC700 and BMBC800 is thought to favor decreasing the distance from microbe than other BMBCs. The results are expected to provide some technical considerations and scientific insight for the optimization of bioreduction by useful microbes combining with biochar composites to be newly developed.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yunfei Zhang,Qiang Zhao,Baoliang Chen
In materials science, “green” synthesis has gotten a lot of interest as a reliable, long-lasting, and ecofriendly way to make a variety of materials/nanomaterials, including metal/metal oxide nanomaterials . To accommodate various biological materials, green synthesis of metallic nanoparticles has been used (e.g., bacteria, fungi, algae, and plant extracts). In this work, Shewanella oneidensis MR-1 was used to biosynthesize palladium nanoparticles (bio Pd) under aerobic conditions for the Cr(VI) bio-reduction. The size and distribution of bio-Pd are controlled by adjusting the ratio of microbial biomass and palladium precursors. The high cell: Pd ratio has the smallest average particle size of 6.33 ± 1.69 nm. And it has the lowest electrocatalytic potential (−0.132 V) for the oxidation of formic acid , which is 0.158 V lower than commercial Pd/C (5%). Our results revealed that the small size and uniformly distributed extracellular bio-Pd could achieve completely catalytic reduction of 200 mg/L Cr(VI) solution within 10 min, while the commercial Pd/C (5%) need at least 45 min. The bio-Pd materials maintain a high reduction during five cycles. Microorganisms play an important role in the whole process, which can fully disperse palladium nanoparticles, completely reduce Cr(VI), and effectively adsorb Cr(III). This work expands our understanding and provides a reference for the design and development of efficient and green bio-Pd catalysts for environmental pollution control under simple and mild conditions.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2018
·
IF 2.776
Chen Zhou,Huiqing Wang,Youbin Si,Kang Wu,Amina Yousaf
The ability of anthraquinone-2,6-disulfonate (AQDS) and riboflavin to enhance the sulfamethoxazole (SMX) degradation coupled with the Fe(III) reduction by Shewanella oneidensis MR-1 was investigated. The results indicated that the SMX degradation rate was 38.5% with an initial SMX concentration at 0.04 mM. For the overall performance of AQDS and riboflavin mediated SMX degradation and iron reduction, the SMX degradation rate was gradually increased with the enhancement of iron reduction. Riboflavin had a stronger enhancement on SMX degradation and iron reduction than AQDS, but the enhancement was not positively correlated with electron shuttles concentration. A quantitative characterization of the electron transfer capacity (ETC) of the electron shuttles showed that the ETC was higher for riboflavin than AQDS. The S. oneidensis MR-1 16S rRNA gene copies results indicated that electron shuttles had a positive effect on the microbial activity of S. oneidensis MR-1. The LC MS result indicated that the products of the SMX biodegradation were 3-amino-5-methylisoxazole and 4-aminobenzenesulfonic acid, which suggested that the SMX biodegradation was caused by S N bond cleavage. This study indicates that the biochemical mechanisms play a vital role in SMX transformation and Fe(II) generation in this system.
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