Environmental Science-Processes & Impacts
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2019
·Vol. 22
·期 1
·197-206
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IF 2.688
Changwei Li,Xianwang Kong,Lihua Lan,Musa Abubakar Tadda,Dezhao Liu
17β-estradiol (E2) ubiquitously exists in various water bodies with long-term endocrine-disrupting and carcinogenic impacts on wildlife even at the trace level of ng L−1. However, it remains unclear how easy-to-degrade carbon sources alter E2 biodegradation patterns. In this study, E2 biodegradation by Sphingomonas sp. MCCC 1A06484 was investigated with regard to alternative carbon sources. Results showed that the bacterium preferentially utilized glucose, sodium succinate and sodium acetate over E2. Interestingly, the presence of these preferred nutrients increased the E2 removal efficiency by 20.1%. Furthermore, a positive relation (p < 0.05) between the utilization of total organic carbon (TOC) and E2 was found. Using intracellular metabolomics by UHPLC-QTOF-MS, 11 up-regulated and 35 down-regulated metabolites (variable importance > 1, p < 0.05) were identified in the bacterium when cultivated with E2 under various carbon and nitrogen backgrounds. The E2 exposure contributed to metabolism changes of lipid, nucleotide, carbohydrate, amino acid and membrane transport, which were considered to play roles in the E2 metabolism. The up-regulated phosphatidylcholine might act as an indicator during the bacterial degradation of E2. Generally, this study contributes to an in-depth understanding of E2 biodegradation in complex environments with multiple carbon and nitrogen sources.
研究领域:
环境科学
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微生物学
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PubMed
高影响因子
Biotechnology Journal
·
2015
·Vol. 11
·期 2
·228-237
·
IF 3.49
Tian Ma,Yuanjie Zhou,Xiaowei Li,Fayin Zhu,Yongbo Cheng,Yi Liu,Zixin Deng,Tiangang Liu
As a highly valued keto-carotenoid, astaxanthin is widely used in nutritional supplements and pharmaceuticals. Therefore, the demand for biosynthetic astaxanthin and improved efficiency of astaxanthin biosynthesis has driven the investigation of metabolic engineering of native astaxanthin producers and heterologous hosts. However, microbial resources for astaxanthin are limited. In this study, we found that the α-Proteobacterium Sphingomonas sp. ATCC 55669 could produce astaxanthin naturally. We used whole-genome sequencing to identify the astaxanthin biosynthetic pathway using a combined PacBio-Illumina approach. The putative astaxanthin biosynthetic pathway in Sphingomonas sp. ATCC 55669 was predicted. For further confirmation, a high-efficiency targeted engineering carotenoid synthesis platform was constructed in E. coli for identifying the functional roles of candidate genes. All genes involved in astaxanthin biosynthesis showed discrete distributions on the chromosome. Moreover, the overexpression of exogenous E. coli idi in Sphingomonas sp. ATCC 55669 increased astaxanthin production by 5.4-fold. This study described a new astaxanthin producer and provided more biosynthesis components for bioengineering of astaxanthin in the future. Graphical Limited microbial resources for astaxanthin limit its widespread applications in nutritional supplements and pharmaceuticals. Sphingomonas sp. ATCC 55669 can produce astaxanthin naturally. Whole-genome sequencing with gene functional confirmation identifies the discrete astaxanthin biosynthetic genes, and overexpressed related genes in E. coli can increase astaxanthin production. The new astaxanthin producer and biosynthesis components provide more possibilities for bioengineering of astaxanthin in the future.
研究领域:
微生物学
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生物工程
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PubMed
高影响因子
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
·
2020
·
IF 1.442
Nengqiang Wang,Zhen Luo,Kaiqin Li,Yingcui Xu,Cheng Peng
A strain LZ1, which showed efficient asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone to enantiopure (S)-[3,5-bis(trifluoromethyl)phenyl]ethanol, which is the key intermediate for the synthesis of a receptor antagonist and antidepressant, was isolated from a soil sample. Based on its morphological, 16S rDNA sequence, and phylogenetic analysis, the strain LZ1 was identified to be Sphingomonas sp. LZ1. To our knowledge, this is the first reported case of the species Sphingomonas exhibiting stricter S-enantioselectivity and its use for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone. Some key reaction parameters involved in the bioreduction catalyzed by whole cells of Sphingomonas sp. LZ1 were subsequently optimized, and the optimized conditions for the synthesis of (S)-[3,5-bis(trifluoromethyl)phenyl]ethanol were determined to be as follows: phosphate buffer pH 7.5, 70 mM of 3,5-bis(trifluoromethyl) acetophenone, 30 g/L of glucose as a co-substrate, 300 g (wet weight)/L of resting cell as the biocatalyst, and a reaction for 24 h at 30°C and 180 rpm. Under the above conditions, a best yield of 94% and an excellent enantiomeric excess of 99.6% were obtained, respectively. Sphingomonas sp. LZ1 could also asymmetrically reduce a variety of prochiral ketones to their corresponding optical alcohols with excellent enantioselectivity. These results indicated that Sphingomonas sp. LZ1 had a remarkable capacity to reduce 3,5-bis(trifluoromethyl)acetophenone to its corresponding (S)-[3,5-bis(trifluoromethyl)phenyl]ethanol, and might be a new potential biocatalyst for the production of valuable chiral alcohols in industry.
研究领域:
微生物学
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生物催化
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有机合成
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PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2018
·
IF 3.641
Bo Ruan,Pingxiao Wu,Xiaolin Lai,Huimin Wang,Liping Li,Liya Chen,Chunxi Kang,Nengwu Zhu,Zhi Dang,Guining Lu
The objective of this study was to investigate the interaction between stearic acid-modified montmorillonite (SA-Mt) and Sphingomonas sp. GY2B and its effect on the physical and chemical properties of montmorillonite in the 2 days' biodegradation of phenanthrene . Due to bacterial activities , surface and colloidal properties of the bacteria-treated sample evidently changed in comparison with the untreated SA-Mt. Changes in surface morphology and the agglomerate state of SA-Mt were clearly observed by scanning electron microscopy (SEM) after interaction with GY2B for 2 days. Although the interlayer space of SA-Mt structure remained basically unchanged, the decrease of the structural order and the appearance and shift of several absorbance bands in the Fourier transform infrared (FTIR) spectra verified the structural changes of SA-Mt after bacterial treatment . Besides, the second derivative infrared spectra indicated that bacterial growth and metabolism largely affected the microstructure in SA-Mt. Dissolution rates of major elements were obtained by inductively coupled plasma optical emission spectroscopy (ICP-OES) in the solution, which revealed that bacterial activities enhanced the release of major elements and brought about a significant higher dissolution of Si preferentially from the tetrahedral sheet edges of SA-Mt than Al. The improvement of elemental dissolution in the biotic experiment was attributed to the combined action of pH effect and ligand-promoted process. These results substantiated that microorganisms have a considerable influence on the physicochemical properties and structure in the clay-modulated biodegradation of organic compounds. This study has profound impacts on the clay-modulated biodegradation of hydrophobic organic contaminants in the environment.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
Microbial Biotechnology
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2024
·
IF 4.8
Mengmeng Wu,Zhuangzhuang Shi,Yue Ming,Yufei Zhao,Ge Gao,Guoqiang Li,Ting Ma
Corn straw is an abundant and renewable alternative for microbial biopolymer production. In this paper, an engineered Sphingomonas sanxanigenens NXG -P 916 capable of co-utilising glucose and xylose from corn straw total hydrolysate to produce xanthan gum was constructed. This strain was obtained by introducing the xanthan gum synthetic operon gum as a module into the genome of the constructed chassis strain NXdPE that could mass produce activated precursors of polysaccharide, and in which the transcriptional levels of gum genes were optimised by screening for a more appropriate promoter, P 916 . As a result, strain NXG -P 916 produced 9.48 ± 0.34 g of xanthan gum per kg of fermentation broth (g/kg) when glucose was used as a carbon source, which was 2.1 times improved over the original engineering strain NXdPE:: gum . Furthermore, in batch fermentation, 12.72 ± 0.75 g/kg xanthan gum was produced from the corn straw total hydrolysate containing both glucose and xylose, and the producing xanthan gum showed an ultrahigh molecular weight (UHMW) of 6.04 × 10 7 Da, which was increased by 15.8 times. Therefore, the great potential of producing UHMW xanthan gum by Sphingomonas sanxanigenens was proved, and the chassis NXdPE has the prospect of becoming an attractive platform organism producing polysaccharides derived from biomass hydrolysates.
研究领域:
生物技术
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微生物学
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生物工程
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可再生资源
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DOI
PubMed
高影响因子
Advanced Science
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2024
·
IF 14.3
Yucheng Zhu,Ruiqi Yang,Zhangchao Deng,Bohua Deng,Kun Zhao,Chen Dai,Gang Wei,YanJiang Wang,Jinshui Zheng,Zhuqing Ren,Wentao Lv,Yingping Xiao,Zhinan Mei,Tongxing Song
Obesity represents a low-grade chronic inflammation status, which is associated with compromised adaptive thermogenesis. However, the mechanisms underlying the defective activation of thermogenesis in chronic inflammation remain unclear. Here, a chronic inflammatory model is first estabolished by injecting mice with low-dose lipopolysaccharide (LPS) before cold exposure, and then it is verified that LPS treatment can decrease the core body temperature of mice and alter the microbial distribution in epididymal white adipose tissue (eWAT). An adipose tissue-resident bacterium Sphingomonas paucimobilis is identified as a potential inhibitor on the activation of brown fat and browning of inguinal WAT, resulting in defective adaptive thermogenesis. Mechanically, LPS and S. paucimobilis inhibit the production and release of 15-HETE by suppressing its main metabolic enzyme 12 lipoxygenase (12-LOX) and 15- Hydroxyeicosatetraenoic acid (15-HETE) rescues the impaired thermogenesis. Interestingly, 15-HETE directly binds to AMP-activated protein kinase α (AMPKα) and elevates the phosphorylation of AMPK, leading to the activation of uncoupling protein 1 (UCP1) and mitochondrial oxidative phosphorylation (OXPHOS) complexes. Further analysis with human obesity subjects reveals that individuals with high body mass index displayed lower 15-HETE levels. Taken together, this work improves the understanding of how chronic inflammation impairs adaptive thermogenesis and provides novel targets for alleviating obesity.
研究领域:
微生物学
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代谢
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免疫学
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内分泌学
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分子生物学
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2017
·
IF 4.9
Naling Bai,Sheng Wang,Pengfei Sun,Rexiding Abuduaini,Xufen Zhu,Yuhua Zhao
In this study, the efficiency of the nonylphenol polyethoxylates (NPEOs)-degrading bacterium Sphingomonas sp. strain Y2 was evaluated, which was immobilized by a novel system composed of polydopamine (PD)-coated Fe 3 O 4 iron nanoparticles (IONPs). The PD-IONPs, with a distinct core-shell structure, relatively uniform size, and high saturation magnetization, were prepared for Y2 immobilization. The performance of Y2 was unaffected by this novel immobilization method, exhibiting 79.5% and 99.9% of NPEOs (500 ppm) degradation efficiency at day 1 and 2, respectively. Furthermore, separation and recycling were more readily achieved for immobilized cells as compared to free cells. Immobilized cells retained over 70% of the original degradation activity after 6 cycles of utilization. These results suggest that Y2-PD-IONPs can be potentially used for NPEOs-contaminated wastewater bioremediation. Capsule Immobilization of Sphingomonas sp. Y2 by functionalized PD-IONPs with easy separation, recycling utilization and high efficiency.
研究领域:
环境科学
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微生物学
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纳米技术
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DOI
PubMed
高影响因子
Catalysts
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2025
·Vol. 15
·期 6
·514
·
IF 4
Xinyu Fan,Pengcheng Chen,Dan Wu,Pu Zheng
Phenylalanine butyramide (FBA) is a novel butyric acid derivative with favorable sensory properties, which has broad prospects in medicine and feed processing. However, there is currently limited research on the enzymatic synthesis of FBA. As is well known, lipase plays a crucial role in amide bond synthesis, but it typically requires completely anhydrous conditions. The lipase fromSphingomonassp. HXN-200 (SpL) is the only intracellular lipase identified to date, capable of catalyzing the ammonolysis of esters or acids in an aqueous phase. In this study, we developed a method for the synthesis of FBA catalyzed by SpL in a biphasic reaction system of water and n-hexane. SpL was successfully expressed inE. coliBL21, and the optimal induction conditions were 0.4 mM IPTG and 18 h. It was ascertained that the n-hexane system containing 2% water was conducive to the reaction. Under optimized reaction conditions, 0.89 mg/mL of FBA was obtained within 15 h at 30 °C. Additionally, we found that SpL also has the ability to hydrolyze amides in the reaction of SpL catalyzing the formation of amides, so we further analyzed its catalytic mechanism.
研究领域:
生物化学
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分子生物学
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生物催化
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应用微生物学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 12
·12019-12029
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IF 2.76
Bai Naling,Wang Sheng,Abuduaini Rexiding,Zhu Xufen,Zhao Yuhua
Nonylphenol polyethoxylates (NPEOs), although banned for decades, are still widely used in manufactories and thus affect human lives. In this study, a highly efficient NPEO-degrading bacterium, Sphingomonas sp. Y2, was isolated from sewage sludge by enrichment culture. Strain Y2 ensured the complete removal of NPEO in 48 h and degraded 99.2 % NPEO (1,000 mg L −1 ) within 30 h at a specific growth rate of 0.73 h −1 in minimum salt medium. To date, this degradation efficiency is the highest reported for NPEO metabolism by a pure bacterium under this condition. Furthermore, the application of this bacterium to wastewater treatment demonstrated that it metabolized 98.5 % NPEO (1,000 mg L −1 ) within 5 days with a specific growth rate of 2.03 day −1 . The degradation intermediates, identified as nonylphenol, short-chain NPEOs and short-chain nonylphenol polyethoxycarboxylates by high-performance liquid chromatography and gas chromatography-mass spectrometry, indicated the sequential exo-cleavage of the EO chain. Additionally, the enzymes involved in the biodegradation were inducible rather than constitutive. Considering that strain Y2 exhibits prominent biodegradation advantages in industrial wastewater treatment, it might serve as a promising potential candidate for in situ bioremediation of contamination by NPEOs and other structurally similar compounds.
研究领域:
环境微生物学
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废物管理
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生物技术
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Jinsong Huang,Tianzheng Bao,Zhaoyu Guo,Yibin Qiu,Mahdy Elsayed,Qun Wu,Meysam Madadi,Hong Xu,Sha Li
Developed a shuttle vector and 60-promoter library for Sphingomonas . • Promoter-controlled lyase expression enabled tunable welan gum fermentation. • Adaptive domestication allowed efficient bagasse hydrolysate utilization. • One-step fermentation produced 28.53 g/L low-MW welan gum sustainably. Sphingomonas represent a significant microbial resource with considerable industrial applicability. However, non-model microorganisms often lack efficient genetic modification tools, which hinders the development of microbial cell factories. In this study, using the welan gum-producing strain Sphingomonas sp. HT-1 as an example, we developed a stable E. coli - Sphingomonas shuttle plasmid pHTK based on endogenous plasmid mining, and the transformation efficiency was enhanced more than 1000-fold by optimizing the transformation conditions. A library of sixty endogenous promoters characterized by distinct strengths and expression dynamics was established. Then, red fluorescent protein expression was successfully achieved in various Sphingomonas species, demonstrating the cross-species applicability of this genetic toolkit. To further improve welan gum synthesis through molecular weight regulation, this toolkit was employed to modulate the expression of a sphingan lyase (SpnR). We demonstrated that the timing of SpnR expression is critical for determining the molecular weight and homogeneity of welan gum. Adaptive domestication enabled S. sp. HT‑1 to efficiently utilize sugarcane bagasse hydrolysate. Expression driven by the strong, late-growth promoter P 3 in S. sp. HT-1 resulted in 28.53 g/L of reduced molecular weight welan gum (255.01 kDa) in a 5 L bioreactor fed with bagasse hydrolysate. This study establishes a genetic toolkit for Sphingomonas and presents an efficient one-step fermentation process for producing low-molecular-weight welan gum, expanding its application prospects. Download: Download high-res image (218KB) Download: Download full-size image
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高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Mengmeng Wu,Xin Zhao,Yaqi Shen,Zhuangzhuang Shi,Guoqiang Li,Ting Ma
Lignocellulosic biomass is a cheap and abundant carbon source in the microbial manufacturing industry. The native co-utilization of glucose and xylose from corn straw total hydrolysate (CSTH) by Sphingomonas sanxanigenens NX02 to produce exopolysaccharide Sanxan was investigated. Batch fermentation demonstrated that, compared to single sugar fermentation, co-substrate of glucose and xylose accelerated cell growth and Sanxan production in the initial 24 h with the same consumption rate. Additionally, NX02 converted CSTH into Sanxan with a yield of 13.10 ± 0.35 g/Kg, which is slightly higher than that of glucose fermentation. Coexistence of three xylose metabolic pathways (Xylose isomerase, Weimberg, and Dahms pathway), incomplete phosphoenolpyruvate-dependent phosphotransferase system, and reinforced fructose metabolism were recognized as the co-utilization mechanism through comparative transcriptome analysis. Therefore, strain NX02 has a prospect of becoming an attractive platform organism to produce polysaccharides and other bio-based products derived from agricultural waste hydrolysate rich in both glucose and xylose.
研究领域:
微生物学
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生物技术
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生化工程
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PubMed
高影响因子
Journal of Environmental Sciences
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2022
·
IF 6.796
Linlin Wang,Nina Wang,Di Guo,Zhengzheng Shang,Yiying Zhang,Song Liu,Yuheng Wang
The ultimate purpose of phytoextraction is not only to remove heavy metals from soil but also to improve soil quality. Here, we evaluated how the joint effect of Streptomyces pactum (strain Act12) and inorganic (Hoagland's solution) and organic (humic acid and peat) nutrients affected the phytoextraction practice of cadmium (Cd) and zinc (Zn) by potherb mustard, and the microbial community composition within rhizosphere was also investigated. The results indicated that the nutrients exerted synergistically with Act12, all increasing the plant biomass and Cd/Zn uptakes. The inoculation of Act12 alone significantly increased dehydrogenase activity of rhizosphere soil ( P < 0.05), while urease and alkaline phosphatase activities varied in different dosage of Act12. Combined application of microbial strain with nutrients increased enzymatic activities with the elevated dosage of Act12. 16S ribosomal RNA high-throughput sequencing analysis revealed that Act12 inoculation reduced the diversity of rhizosphere bacteria. The Act12 and nutrients did not change dominant phyla i.e. , Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria and Acidobacteria, but their relative abundance differed among the treatments with: Peat > Act12 > Humic acid > Hoagland's solution. Comparatively, Sphingomonas replaced Thiobacillus as dominant genus after Act12 application. The increase in the Sphingomonas and Flavisolibacter abundances under Act12 and nutrients treatments gave rise to growth-promoting effect on plant. Our results revealed the important role for rhizosphere microbiota in mediating soil biochemical traits and plant growth, and our approach charted a path toward the development of Act12 combined with soil nutrients to enhance soil quality and phytoextraction efficiency in Cd/Zn-contaminated soils.
研究领域:
环境科学
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微生物学
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土壤学
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植物科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）