Scientific Reports
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2020
·Vol. 10
·期 1
·1-4
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IF 3.998
Jing Yun-Rong,Zhou Wei,Wang Xiang-Yang
In an attempt to improve the oral bioavailability of taxanes, a series of new analogues were synthesized and tested in a panel of human tumor cell lines and cellular permeability assays. Compounds T-13 and T-26 showed potent cytotoxicity and exhibited the highest permeability, so they were selected for pharmacokinetic studies. Here, pharmacokinetics of T-13 and T-26 were studied after intravenous injection (5 mg/kg) and oral administration (60 mg/kg) in male Sprague-Dawley (S.D.) rats, respectively. Plasma concentrations were characterized using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The oral bioavailability of T-13 and T-26 was determined to be 10.71% and 65.79%, respectively. Compounds T-13 and T-26 were both poor substrates of P-glycoprotein (P-gp), and they had a much higher bioavailability than paclitaxel, especially T-26. T-26 with good oral bioavailability represented a potential candidate for potent antitumor activity given oral administration.
研究领域:
药理学
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肿瘤学
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药物代谢
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 28
·7001-7007
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IF 4.157
Sang Fuming,Yin Suyao,Pan Jianxin,Zhang Zhizhou
A novelty aptasensor for ultrasensitive detection of Hg 2+ is developed, exploiting the combination of plasmonic properties of gold nanoparticles (AuNPs) and exonuclease III (Exo III)-assisted target recycling for signal amplification. In the presence of Hg 2+ , a DNA duplex can be formed due to the strong coordination of Hg 2+ and T bases of single-stranded DNA (ssDNA) probe. Exo III digests the DNA duplex from the 3′ to 5′ direction, resulting in the releasing of Hg 2+ . Then, the released Hg 2+ binds with another ssDNA probe through T-Hg 2+ -T coordination. After Exo III-assisted Hg 2+ cycles, numerous ssDNA probes are exhausted, which promotes poly(diallyldimethylammonium chloride) (PDDA)-induced AuNP aggregation, leading to an obvious color change and aggregation-induced plasmon red shift of AuNPs (from 520 to 610 nm). Therefore, this biosensor is ultrasensitive, which is applicable to the detection of trace level of Hg 2+ with a linear range from 5 pM to 0.6 nM and an ultralow detection limit of 0.2 pM. Furthermore, it enables visual detection of Hg 2+ as low as 50 pM by the naked eye. More importantly, the assay can be applied to the reliable determination of spiked Hg 2+ in sea water samples with good recovery.
研究领域:
生物传感
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环境科学
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纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Zhao Weiqin,Huang Chun,Guo Xiyu,Zhu Youyu,Li Yuangang,Duan Yingfeng,Gao Jie
Mercury ions (Hg 2+ ) can cause damage to human health, and thus, the study of the detection of Hg 2+ is extraordinarily important in daily life. This work reported a fluorescence biosensor for the detection of Hg 2+ . The key point of this strategy was that the fluorescence of carbon quantum dots made from pomegranate peel (P-CQDs) was quenched by hemin, and restored after G-quadruplex binding with hemin. The presence of Hg 2+ caused thymine (T)-rich DNA fragments to form T-Hg 2+ -T mismatches, and this change allowed the release of G-quadruplex. G-quadruplex could change the fluorescence of hemin/P-CQDs. P-CQDs exhibited excellent properties through characterization analysis, such as transmission electron microscope, X-ray photoelectron spectroscopy and Fourier transform infrared. This proposed fluorescence detection strategy established the linear ranges of Hg 2+ from 1 nM to 50 nM. In conclusion, this simple biosensor had the advantages of strong sensitivity, high selectivity, and low cost for Hg 2+ detection in environmental water samples.
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Huijuan Ma,Tingqian Wang,Junfeng Wang,Peiyao Wang,Qi Shu,Ruilin Qin,Sijia Li,Huan Xu
The correlation between formaldehyde (FA) exposure and prevalence of asthma has been widely reported. However, the underlying mechanism is still not fully understood. FA exposure at 2.0 mg/m 3 was found to exacerbate asthma in OVA-induced murine models. IFN-γ, the cytokine produced by T helper 1 (Th1) cells, was significantly induced by FA in serum and bronchoalveolar lavage fluid (BALF) of asthmatic mice, which was different from cytokines secreted by other Th cells. The observation was also confirmed by mRNA levels of Th marker genes in CD4+ T cells isolated from BALF. In addition, increased production of IFN-γ and expression of T-bet in Jurkat T cells primed with phorbol ester and phytohaemagglutinin were also observed with 100 μM FA treatment in vitro . Upregulated STAT1 phosphorylation, T-bet expression and IFN-γ production induced by FA was found to be restrained by STAT1 inhibitor fludarabine, indicating that FA promoted Th1 commitment through the autocrine IFN-γ/STAT1/T-bet pathway in asthma. This work not only revealed that FA could bias Th lineage commitment to exacerbate allergic asthma, but also identified the signaling mechanism of FA-induced Th1 differentiation, which may be utilized as the target for development of interfering strategies against FA-induced immune disorders.
研究领域:
免疫学
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呼吸病学
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毒理学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2019
·
IF 5.256
Zhihong Li,Hongjing Sun,Xinyue Ma,Ruifang Su,Rui Sun,Chuanyu Yang,Chunyan Sun
A novel label-free fluorescence “turn-on” strategy was developed for the sensitive detection of Hg 2+ based on the thymine-Hg 2+ -thymine (T-Hg 2+ -T) coordination and the fact that single-stranded DNA (ssDNA) greatly enhances the fluorescence of terbium (III) (Tb 3+ ), but double-stranded DNA (dsDNA) does not. In the absence of Hg 2+ , the mercury-specific DNA (MSD) hybridized with the corresponding complementary strand (cDNA) to form a double helix structure in solution based on Watson-Crick base pairings, which cannot enhance the fluorescence of Tb 3+ . In the presence of Hg 2+ , MSD preferentially bound with Hg 2+ to form the T-Hg 2+ -T complex due to the strong affinity of Hg(II) for the T bases of DNA, thus avoiding the hybridization of the cDNA to MSD. The free cDNA can greatly enhance the emission of Tb 3+ , leading to a sharp increase in fluorescence intensity . Under the optimized conditions, the increased fluorescence intensity was proportional to the concentration of Hg 2+ in the range from 10 to 600 nM, and this method can detect concentrations of Hg 2+ as low as 0.24 nM. Moreover, satisfactory results were obtained for the detection of Hg 2+ in river water and fish samples, and the results were consistent with those from the atomic fluorescence spectroscopy (AFS). Thus, the fluorescence characteristics of Tb 3+ were here used in a “turn on” approach to detect Hg 2+ , which represents a new opportunity for Hg 2+ analysis in the field of environmental monitoring and food safety.
研究领域:
环境科学
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食品安全
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分析化学
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DOI
PubMed
高影响因子
MEDICAL SCIENCE MONITOR
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2019
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IF 1.98
Liang Chen,Hanfei Huang,Weixin Zhang,Feifan Ding,Zhenlei Fan,Zhong Zeng
Background CD8 + cytotoxic T lymphocytes (CTLs) have been proved to exert crucial roles in immunological rejection. Exosomes (EXOs) secreted by CD4 + CD25 + regulatory T (Treg) cells is believed to be deeply involved in immune regulation. Nevertheless, whether immunomodulatory effect of CD4 + CD25 + Treg cells on CD8 + CTL depends on EXOs remains unknown and needs to be explored. Material/Methods We purified CD4 + CD25 + Treg cells followed by in vitro amplification. EXOs in culture supernatants of Treg cells was separated and identified. The effect of CD4 + CD25 + Treg cells and CD4 + CD25 + Treg cells-derived EXOs on CD8 + CTL viability, proliferation, cell cycle mRNA, intracellular cytokines, and protein expression were investigated. Results We successfully obtained EXOs from CD4 + CD25 + Treg cells. The inhibition effect of EXOs on CD8 + CTL was concentration-dependent. In addition, the inhibition effect of CD4 + CD25 + Treg cells could be reversed by GW4869, an EXOs inhibitor. The inhibition effect was associated with the regulation of IFN-γ and perforin. Our in vivo experiments proved that natural CD4 + CD25 + Treg cells-released EXOs can prolong liver allograft survival. Conclusions CD4 + CD25 + Treg cells-derived EXOs could become an alternative tool for manipulating the immune system to discover novel underlying immunomodulatory mechanisms.
研究领域:
免疫学
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移植医学
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细胞生物学
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DOI
PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2020
·Vol. 126
·期 4
·1-10
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IF 1.81
Wu Guangfeng,Liu Chang,Lu Congcong,Zhang Huixuan
In order to protect the articles in buildings from ultraviolet radiation and save air-conditioning energy, polyvinyl butyral laminated glass are employed and produced in large quantities. Several inorganic particles with blocking effect have been doped into PVB, such as TiO 2 , ZnO, and ATO. T-ZnOw is a widely used functional structural material with excellent absorption properties. Herein, T-ZnOw was lipophilic modified with silane coupling agent KH-570, and T-ZnOw/PVB composite films were prepared via subsequent solution blending. As per the FTIR spectrum, a characteristic peak of Si–O–Zn at 1165 cm −1 was observed, indicating that T-ZnOw was successfully modified by KH-570. T-ZnOw before and after modification was separately mixed with the St/H 2 O mixed solution. The modified T-ZnOw could be dispersed in styrene instead of water, displaying a certain lipophilicity. On the other hand, from a microscopic perspective, the SEM images suggest that filling the filler in the form of a suspension can disperse the modified T-ZnOw in PVB in an independent four-needle form, which contributes toward improvement of the agglomeration of T-ZnOw in the PVB matrix. The UV–Vis–NIR spectrum shows that the addition of T-ZnOw reduced the UV transmittance of T-ZnOw/PVB composite films from 50.84 to 25.77%, resulting in a decrease in near-infrared transmittance from 82.14 to 43.22%. The ultraviolet and near-infrared light barrier properties of composite films were more than twice that of the ordinary PVB films. Moreover, the modified T-ZnOw endowed composite films with stronger ultraviolet light and near-infrared light-blocking properties, or better visible light transmittance. And the falling ball impact test proved that there was no clear relationship between the thickness of the film and the adhesion. This work proposes a new solution and preparation method for the production of energy-saving glass. Therefore, it is expected that using composite films instead of traditional PVB films to make laminated glass can greatly contribute toward energy conservation and environmental protection.
研究领域:
材料科学
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光学工程
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DOI
高影响因子
Analytical Methods
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2024
·Vol. 16
·期 29
·4951-4959
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IF 2.7
Weiqin Zhao,Ruichen Zhai,Qianxiao Chen,Chun Huang,Haojia Li,Youyu Zhu,Yingfeng Duan,Jie Gao
Glutathione (GSH) is commonly used as a diagnostic biomarker for many diseases. In this study, based on carbon quantum dots prepared from dragon fruit peel (D-CQDs) and the T-Hg(II)-T mismatch, a dual-mode biosensor was developed for the detection of GSH. This system consists of two single-stranded DNA (ssDNA). DNA1 was the T-rich sequence; DNA2 was attached to streptavidin-coated magnetic beads and consisted of T-rich and G-rich fragments. Due to the presence of Hg(II), the T-Hg(II)-T mismatch was formed between T-rich fragments of two ssDNA. In the presence of GSH, Hg(II) detached from dsDNA and bound with GSH to form a new complex. The G-rich fragment assembled with the hemin shed from D-CQDs to form the G-quadruplex/hemin complex. At this time, in fluorescence mode, the fluorescence of D-CQDs quenched by hemin could be restored. In colorimetric mode, after the magnetic beads separate, a visual signal could be produced by catalyzing the oxidation of ABTS using the peroxide-like activity of the G-quadruplex/hemin complex. This biosensor in both fluorescence mode and colorimetric mode had excellent selectivity and sensitivity, and the limit of detection was 0.089 µM and 0.26 µM for GSH, respectively. Moreover, the proposed dual-mode biosensor had good application prospects for detection of GSH.
研究领域:
生物化学
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纳米生物学
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分析化学
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PubMed
高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 186
·期 5
·1-6
·
IF 5.479
Ma Haiyan,Xue Ning,Wu Shujie,Li Zongbing,Miao Xiangmin
The authors describe a method for detection of Hg 2+ by using positively charged gold nanoparticles ((+)AuNPs) as a quencher of the fluorescence of DNA-capped silver nanoclusters (DNA-AgNCs) which are negatively charged. In the presence of Hg 2+ , a DNA duplex is formed through T-Hg 2+ -T coordination chemistry. The duplex can be digested by exonuclease III to form smaller DNA fragments. This leads to the release of the AgNCs and the recovery of fluorescence, best measured at excitation/emission wavelengths of 460/530 nm. The (+)AuNPs and Hg 2+ are also released and can be reused for target recycling signal amplification. Based on these findings, a method is worked out for the determination of Hg 2+ that works in the 5.0 pM to 10 nM concentration range and has a detection limit as low as 2.3 pM. It is highly selective because of the highly specific formation of T-Hg 2+ -T bonds.
By using ultrastable and positively charged gold nanoparticles as fluorescence quenchers and exonuclease assisted signal amplification, a method is developed for the sensitive and selective detection of Hg 2+ in water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2022
·
IF 7.033
An-Ran Shen,Xiao-Xiao Jin,Tao-Tao Tang,Yan Ding,Xiao-Tao Liu,Xin Zhong,Yan-Dan Wu,Xue-Lian Han,Guang-Yu Zhao,Chuan-Lai Shen,Lin-Li Lv,Bi-Cheng Liu
Purpose Current vaccines for the SARS-CoV-2 virus mainly induce neutralizing antibodies but overlook the T cell responses. This study aims to generate an exosomal vaccine carrying T cell epitope peptides of SARS-CoV-2 for the induction of CD8 + T cell response. Methods Thirty-one peptides presented by HLA-A0201 molecule were conjugated to the DMPE-PEG-NHS molecules, and mixed with DSPE-PEG to form the peptide-PEG-lipid micelles, then fused with exosomes to generate the exosomal vaccine, followed by purification using size-exclusion chromatography and validation by Western blotting, liquid nuclear magnetic resonance (NMR) test and transmission electron microscopy. Furthermore, the exosomal vaccine was mixed with Poly (I:C) adjuvant and subcutaneously administered for three times into the hybrid mice of HLA-A0201/DR1 transgenic mice with wild-type mice. Then, the epitope-specific T cell responses were detected by ex vivo ELISPOT assay and intracellular cytokine staining. Results The exosomal vaccine was purified from the Peak 2 fraction of FPLC and injected into the hybrid mice for three times. The IFN-γ spot forming units and the frequencies of IFN-γ + /CD8 + T cells were 10–82-fold and 13–65-fold, respectively, higher in the exosomal vaccine group compared to the Poly (I:C) control group, without visible organ toxicity. In comparison with the peptides cocktail vaccine generated in our recent work, the exosomal vaccine induced significantly stronger T cell response. Conclusion Exosomal vaccine loading T cell epitope peptides of SARS-CoV-2 virus was initially generated without pre-modification for both peptides and exosomes, and elicited robust CD8 + T cell response in HLA-A transgenic mice.
研究领域:
免疫学
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病毒学
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分子生物学
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疫苗研究
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Junyang Wang,Caiyi Du,Peitong Yu,Qian Zhang,Hongxia Li,Chunyan Sun
A label-free and enzyme-free signal-on biosensor for Hg(II) ions was constructed based on T-Hg(II)-T nanoladders and fluorescent DNA-templated silver nanoclusters/graphene oxide nanocomposites (DNA-AgNCs/GO). The T-rich sequences (P1 and P2) were used to capture Hg(II) and form the T-Hg(II)-T nanoladders. C 6 G 5 C 6 was extended at the 5′ end of P1, used as a template for synthesizing fluorescent DNA-AgNCs. In the presence of mercury ions , the T-Hg(II)-T nanoladders significantly enhanced the emission of AgNCs. GO can attract free P1-C 6 G 5 C 6 and P2 that were not involved in the formation of T-Hg(II)-T nanoladders, thus employed as the energy acceptor to quench the background fluorescence of AgNCs. The fluorescence enhancement induced by mercury ions can be used for quantitative detection of Hg(II) over the range 0.1–30 nM with a detection limit as low as 7.35 pM. This method was used to detect Hg(II) in the spiked samples of Songhua River water and grass carp, with the recoveries of 97.61%− 103.79% and 96.52%− 105.58% respectively, consistent with the results from atomic fluorescence spectrometry. Obviously, in this assay, enzyme-free signal amplification and signal identification are integrated in the ingenious design of nucleic acid sequences, thus achieving the label-free fluorescence detection of mercury ions.
研究领域:
生物化学
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环境科学
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DOI
高影响因子
Desalination and Water Treatment
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2024
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IF 1
Cheng-Han Lee,Wei-Sheng Chen,Jun-Yi Wu
Cesium can be widely used in many industries now. However, the primary reservoir of cesium is often located in high mountains and deserts, so it is challenging to obtain cesium resources. To solve this problem, cesium was recovered from waste desalination brine through Dowex G26 resin in this study. Due to the lack of water resources, desalination technology was developed since the 20th century. Nevertheless, its by-product, brine, is harmful to the environment. To reduce the threat of waste brine, this experiment aims to provide a simple procedure to recover cesium resources from brine. The study could be divided into three parts. First, the adsorption isotherms and the thermodynamics parameters such as enthalpy, entropy, and Gibbs free energy of cesium adsorption were investigated. In the second part, the batch experiments would explore the optimal adsorptive period, pH value, and liquid-solid ratio (L/S ratio) of cesium adsorption through Dowex G26 resin. The results showed that the adsorption of cesium fit with the Langmuir model, and the reaction was spontaneous. On the other hand, cesium could be recovered efficiently under the conditions of 4 min of contacting time, pH 9, and L/S ratio of 1,000. After adsorbing, the cesium could be desorbed through nitric acid, and solid cesium nitrate would be obtained by drying the desorbed solutions. In the end, the Dowex G26 system would be compared with the t-BAMBP/kerosene system and t-BAMBP/C 2 mimNTf 2 system to demonstrate its advantages and drawbacks.
研究领域:
环境科学
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化学工程
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材料科学
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资源回收技术
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吸附技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）