FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhao-Rong Li,Rui-Bo Jia,Xueyuan Cai,Donghui Luo,Chong Chen,Mouming Zhao
The current study was aimed to enhance the solubility, dispersibility and biotransformation efficacy of ellagic acid (EA) by preparing food-derived ellagic acid- Undaria pinnatifida polysaccharides solid dispersion (EA/UPP SD). The results demonstrated that the solubility of EA/UPP SD was improved from 0.014 mg/mL to 0.383 mg/mL, and the enhancement was related to converting to a more amorphous state and restraining its self-aggregation during the mechanochemical process. The structure of EA/UPP SDs was mostly maintained by hydrogen bonds and hydrophobic interactions between EA and UPP . Moreover, the result of in vitro anaerobic incubations showed the biotransformation process was improved with EA/UPP SD addition to substrate due to the advance of microbial accessibility in EA dispersion. Altogether, these results indicated that the EA/UPP SDs expanded the application of EA by increasing the solubility and dispersity, and provided a theoretical basis for bioconversion efficiency enhancement.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Ziyan Wang,Heping Cui,Mengyu Ma,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The effect of ellagic acid on the formation of pyrazine, methylpyrazine, 2,3-methylpyrazine, 2,6-methylpyrazine, 2,5-methylpyrazine, and trimethylpyrazine in the xylose–glycine Maillard reaction model was researched. Ellagic acid could either inhibit or promote pyrazine formation, depending on its addition time point and the pH of the system. The addition of ellagic acid during the accumulation period of an Amadori compound inhibited pyrazine formation by capturing the Amadori compound in the xylose–glycine Maillard system and decreasing the pyrazine precursors. The inhibitory effect of ellagic acid on pyrazine formation got more obvious with an increase in the pH of the system. However, when ellagic acid was added at the beginning of the xylose and glycine Maillard system and when the oxidizing substances such as glyoxal and methylglyoxal were significantly formed in the Maillard system, its oxidation could promote the formation of pyrazines.
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LANGMUIR
·
2026
·
IF 4.4
Dang Chunlei,Jiang Jing,Zhao Lin,Zhang Yagang
The
anthropogenic acceleration of the nitrogen cycle has exacerbated
nitrate contamination in water bodies, posing threats to both ecological
balance and public health. Conventional physicochemical separation
and biological denitrification technologies suffer from high costs,
secondary pollution, or resource wastage. The electrochemical nitrate
reduction reaction (NO 3 RR) offers a promising route to
convert nitrate into value-added ammonia, combining environmental
remediation with resource recovery. However, its application is hindered
by sluggish multielectron transfer kinetics and competition from the
hydrogen evolution reaction (HER). The purpose of this study is to
develop a high-performance NO 3 RR electrocatalyst with precise
regulation of metal ion distribution by EA, to promote the metal-polyphenol
self-assembly system from the perspective of ligand engineering, and
to provide new insights for the rational design of advanced heterogeneous
electrocatalysts. Building on the demonstration that the metal-polyphenol
assembly strategy enhances the catalytic performance of Co 3 O 4 , we further introduced ellagic acid (EA), a small-molecule
polyphenol, to coordinate and self-assemble with transition metal
ions of different valence states (Fe 3+ , Ni 2+ , Cu 2+ ). A series of EA-M/Co 3 O 4 (M
= Fe, Ni, Cu) catalysts was constructed to investigate the effects
of polyphenol molecular structure and metal substitution mode on the
structure and performance of Co 3 O 4 . The results
show that the system exhibits excellent catalytic activity: EA-Cu/Co 3 O 4 achieves a Faradaic efficiency of 96.83% at
−0.3 V vs RHE and an NH 3 yield of 279.43 μmol
h –1 cm –2 at −0.5 V vs RHE.
Furthermore, density functional theory (DFT) calculations elucidate
the NO 3 RR mechanism on EA-Cu/Co 3 O 4 . Cu 2+ doping modulated the electronic structure, enhanced
the adsorption of reactants, and thereby promoted the NO 3 RR process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Chunjun Yan,Zheng Zhou
Prion diseases are fatal neurodegenerative diseases caused by the conformational transition of the cellular prion protein (PrP C ) to the abnormal pathological prion protein (PrP Sc ). In this work, the effects of ellagic acid (EA) and pentagalloylglucose (PGG) on prion protein (PrP) fibrillization were investigated. Fluorescence quenching experiments indicated that both EA and PGG could specifically interact with native human PrP with binding affinities of 1.92 × 10 5 and 2.36 × 10 5 L·mol −1 , respectively. Thioflavin-T (ThT) fluorescence assays showed that the binding of EA or PPG could effectively inhibit the nucleation and elongation of PrP fibrilization and reduce the amount of PrP fibrils generated. EA and PGG could also lead to a significant disaggregation of PrP fibrils. Circular dichroism (CD) measurements suggested that EA- or PPG-bound PrP could preserve a higher content of α-helical structures than β-sheet-rich PrP fibrils. The PrP aggregates formed in the presence of EA or PGG showed lower resistance to proteinase K (PK) digestion. Overall, the present work reported the inhibitory effect of EA and PGG on PrP fibrillization. These two natural polyphenols could be potential prodrug molecules for the prevention and treatment of prion diseases.
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CHEMICAL ENGINEERING & TECHNOLOGY
·
2018
·Vol. 41
·期 6
·1188-1198
·
IF 1.588
Li Wang,Bolin Lian,Weiwei Wu,Yiping Deng,Yanjie Liu,Lingling Wang,Yuanyuan Li,Zijian Wang,Xiuhua Zhao
Ellagic acid (EA) was produced from pomegranate husk by extraction of tannins followed by acid hydrolysis and purified by antisolvent recrystallization. Response surface methodology, based on a five-level, four-variable central composite design, was employed to find the optimum purification conditions. Under these conditions, high purity and yield of EA was obtained in close agreement with the predicted values by the model. Analyzes of surface morphology and in vitro percutaneous absorption experiments of EA let conclude that the granule morphology of the purified EA was significantly different from the crude EA, and its particle size was much smaller than that of the crude EA. Evaluation in vitro demonstrated that the permeation rate of the purified EA was higher compared with the crude EA.
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Biomaterials Advances
·
2025
·
IF 6
Yinan Wang,Xiuxiu Huang,Xiao Luo,Meizhen He,Yuqi Fang,Dongzhi Yang
Oxidative stress plays a key role in inflammatory diseases like colitis, but traditional antioxidants are limited by poor bioavailability and non-specific actions, reducing their effectiveness. This study seeks to overcome these limitations by creating a targeted nanotherapy that combines natural ellagic acid with bismuth-based metal-organic framework (MOF) chemistry. SU-101, a MOF, was synthesized through the coordination of ellagic acid and bismuth acetate. SU-101, with a rod-like shape and a zeta potential of −38.51 mV, effectively targets inflamed colonic tissue. It shows strong radical scavenging and SOD/CAT-mimetic activities, providing dual therapeutic benefits. At 5 mg/kg orally, SU-101 outperforms 5-aminosalicylic acid (5-ASA) by reducing weight loss, lowering pro-inflammatory cytokines (IL-6, TNF-α), restoring intestinal barrier proteins, and altering gut microbiota and arginine metabolism. As the ellagic acid-derived bismuth MOF, SU-101 offers precise, multi-level treatment for oxidative inflammation, with promising oral bioavailability and microbiome remodeling potential for inflammatory bowel disease therapy.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Xiaoqin Diao,Mei Tian,Shulei Li,Gaojie Zhao,Nike Tian,Qiao Guan,Dengyong Liu,Haining Guan
Lipoxygenase (LOX)-induced endogenous lipid peroxidation significantly contributes to food nutrient loss and flavor deterioration. This study aimed to investigate the inhibitory mechanism of ellagic acid (EA) on LOX by integrating enzyme kinetics, multi-spectroscopic techniques, and molecular docking. The results showed that EA inhibited LOX in a dose-dependent manner, achieving an inhibition rate of 87.73% at 600 μg/mL and an IC 50 of 20.84 μg/mL. The inhibition was identified as mixed-type competitive inhibition. EA effectively quenched the endogenous fluorescence of LOX through static quenching, and the association constant increased with rising temperature. Thermodynamic parameters suggested that the formation of the EA-LOX complex was spontaneous and endothermic, primarily driven by hydrophobic interactions. EA preferentially interacted with tryptophan residues, inducing changes in the microenvironment and secondary structure of LOX. Additionally, an increase in the average particle size confirmed complex formation. The molecular docking results were consistent with the spectroscopic experiments, providing a possible microscopic mechanism for the maintenance of the three-dimensional conformation of the complex through hydrophobic interactions and hydrogen bonding. This study provides a theoretical foundation for the use of EA to regulate LOX activity and highlights its promising application in food quality preservation.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yang Yang,Pui-Kei Lee,Ho-Ching Wong,Danyue Zhao
Ellagic acid (EA) is an abundant dietary polyphenol with multifarious health benefits but low bioavailability. To increase its bio-efficacy, converting EA to urolithins by supplementing urolithin-producing bacteria, e.g., Gordonibacter urolithinfaciens ( G.uro ), could be a solution. This work first tested three methods for oral delivery of live G.uro . Intestinal colonization of G.uro and its impact on local gut microbiota, EA-to-urolithin conversion and bioavailability were then investigated in C57BL/6J mice administered to EA only or a synbiotic ( G. uro + EA). In vitro results suggested that G.uro largely survived simulated gastrointestinal digestion and could be administered without protection. In vivo results showed that G.uro had little impact on local gut microbiota but could not colonize the gut. Moreover, synbiotic remarkably promoted Akkermansia , Lactobacillus and Bifidobacterium growth, and significantly enhanced the bioavailability of urolithins compared with the EA-only group. This study demonstrated the potential of oral supplementation of G.uro for enhancing EA-to-urolithin bioconversion and bioavailability.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Minglu Zhang,Yi Zhang,Zhi-feng Cai
Development of beneficial sensors to analyze ellagic acid concentrations is of great importance for food safety and human health. Herein, a facile and fast fluorescent probe was carried out for the excellently selective and sensitive measurement of ellagic acid in real samples through histidine protected copper nanoclusters (histidine@Cu NCs) as a nanosensor. This as-developed histidine@Cu NCs were performed through UV–vis absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy and fluorescence lifetime analysis. The TEM image revealed that this nanomaterial had spherical features with the average diameter of 2.5 ± 0.05 nm. The blue-green fluorescence of this Cu NCs was found under the UV light. Meanwhile, the maximum excitation and emission wavelength were located at 387 nm and 488 nm. After addition of ellagic acid, the fluorescence of histidine@Cu NCs was slowly weakened with excellent linear range of 0.5–300 μM and detection limit of 0.077 μM. The fluorescence weakening mechanism of this nanosensor were attributed to the inner filter effect (IFE) and static quenching. Finally, this as-established analysis platform was successfully employed to measure ellagic acid in real samples.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2019
·Vol. 43
·期 11
·e12996
·
IF 1.358
Qian Huang,Wei-Ming Chai,Zuo-Yuan Ma,Wei-Liang Deng,Qi-Ming Wei,Shuang Song,Zheng-Rong Zou,Yi-Yuan Peng
The activities of ellagic acid in inhibiting mushroom tyrosinase and cell proliferation were evaluated in this research. The results of enzyme kinetics indicated that ellagic acid could effectively inhibit tyrosinase activity. The value of the semi-inhibitory rate (IC 50 ) was 0.2 ± 0.05 mM. Ellagic acid inhibited tyrosinase activity in a reversible manner and was a mixed tyrosinase inhibitor. Furthermore, ellagic acid had a good inhibitory effect on the proliferation of mouse melanoma B 16 cells and could induce apoptosis. The results acquired from fluorescence spectroscopy revealed that the interaction of ellagic acid with tyrosinase depended on hydrogen bond and electrostatic force. In addition, computational docking showed that ellagic acid interacted with amino acid residues of tyrosinase (Asn19 and Lys372) by hydrogen bond and produced electrostatic interaction with amino residue Lys18. Practical applications In the present research, the antityrosinase mechanism of ellagic acid and its effect on mouse melanoma cells were investigated. This study suggested that ellagic acid had a strong inhibitory activity against tyrosinase and cell proliferation,which laid an experimental foundation for the development of new drugs and whitening products. The combined multispectral methods used in this research can be applied to the screening of other antityrosinase inhibitors, further promoting the development and utilization of tyrosinase inhibitors.
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Food Bioscience
·
2024
·
IF 4.8
Junfeng Li,Min Guo,Cuiping Yuan,Tiezhu Li,Jie Zhang,Li Ren
ABSTRACT Peroxisome proliferator-activated receptor γ (PPARγ) was a major transcription factor and performed an essential function in the regulation of lipid metabolism. Ellagic acid (EA) was a natural phytochemical extracted from berries and pomegranates, and research had demonstrated its ameliorative effects on lipid metabolism disorders. We intended to explore the interventional effect and mechanism of action of EA on hyperlipidemia. In vitro experiments revealed that EA could reduce lipid accumulation in HepG2 cells. EA was found to bind stably to PPARγ, suppressing its nuclear translocation as well as its luciferase activity. It could also down-regulate the expression of genes and proteins related to lipid metabolism upstream and downstream of PPARγ. To explore the impact of EA on hyperlipidemia in vivo from different levels and perspectives, we established hyperlipidemia mice and hyperlipidemia zebrafish models. Consistent with the in vitro results, in vivo experiments revealed that EA attenuated lipid accumulation in hyperlipidemia mice and reduced the levels of related inflammatory factors in their serum. EA also inhibited lipid accumulation by decreasing the expression of upstream and downstream lipid-associated genes of pparg in hyperlipidemia zebrafish. In conclusion, EA could effectively improve hyperlipidemia by regulating PPARγ.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Xue Zhang,Chunyi Luo,Na Liu,Siyu Liu,Zhouquan Jiang,Jinjun Zhou,Lishu Shao,Weihua Luo
To enhance the clinical value of carboxymethyl chitosan (CMCS) as an osteogenic material, improving its bioactivity and mechanical strength is crucial. Meanwhile, ellagic acid (EA) has strong bioactivities but low bioavailability due to its hydrophobicity. In this study, by introducing EA units into CMCS molecules, a near-infrared (NIR) light-mediated nanofiber membrane based on polycaprolactone (PCL)/EA-CMCS blended nanofibers coated with an EA-CMCS/graphene oxide (GO) layer (PCL/EA-CMCS@EA-CMCS/GO) was designed. The bioactivity of CMCS and bioavailability of EA could be significantly improved by covalent coupling of EA with CMCS. Blending EA-CMCS with PCL significantly enhanced the mechanical properties of nanofiber membranes. The coated nanofibrous membrane featured a multi-level structure of coating and blending. By leveraging the photothermal conversion effect of GO, it enabled the programmed and sustained release of EA-CMCS from both the coating and the nanofibers. Moreover, the membrane exhibited excellent biomineralization and antioxidant capabilities. It effectively regulated macrophage polarization and promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 osteoblasts. These properties enabled the membrane to regulate the harsh osteogenic immune microenvironment and promote bone regeneration. This biomimetic periosteum holds great potential as a substitute for periosteal grafts and offers a novel strategy for developing artificial periosteum.
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