ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Shuai Wu,Jingmiao Zhang,Ao Xia,Yun Huang,Xianqing Zhu,Xun Zhu,Qiang Liao
Microalgae-based technology provides a potential approach to biologically treating oxytetracycline (OTC) wastewater due to its environmental friendliness, low cost, and high efficiency. However, the OTC degradation and transformation characteristics by microalgae are still unclear and need further exploration. This study used microalgae Chlorella sorokiniana MB-1 for OTC wastewater treatment . The OTC with an initial concentration less than 50 mg L −1 promoted microalgae growth, while OTC with a concentration higher than 100 mg L −1 inhibited microalgae growth significantly. More than 99% OTC was removed with the biomass productivity up to 1.8 g L −1 when treated OTC with 10 mg L −1 initial concentration for 7 days. Chlorophyll and total sugar contents decreased, while protein and lipid contents increased compared to the control without OTC. The malondialdehyde content firstly reduced but subsequently enhanced when increased OTC concentration, while superoxide dismutase content gradually enhanced, manifesting that traces of OTC stimulate microalgae antioxidant capacity, while the increasing OTC caused further oxidative damage to microalgae cells. The removal pathways of OTC mainly include photolysis (75.8%), biodegradation (17.8%), biosorption (3.6%), and hydrolysis (2.7%). Overall, removing OTC by microalgae was confirmed to be an excellent technology for treating antibiotics wastewater whilst accumulating microalgae biomass.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Meiting Kang,Qiang Zhao
Oxytetracycline (OTC) is one commonly used antibiotic, and its overuse has led to contamination in environment and food, posing substantial risks to both human health and ecology systems. Development of sensitive and rapid methods for OTC detection is in demands. High-affinity DNA aptamers (OTC5) for OTC recently have been selected, providing new tools for OTC detection. In this work, based on aptamer-binding induced increase of fluorescence anisotropy (FA) of OTC, we utilized FA analysis to systematically characterize affinity of aptamers and mutant sequences. We identified the key regions of OTC5 aptamer for OTC recognition and conserved bases of aptamer sequence by fully testing single base mutation. In addition, we found the OTC5 aptamer with mutation of C10 base to deoxyuridine (dU) (OTC5-C10dU) showed enhanced affinity. We further developed a direct FA sensor for OTC detection by using this mutated aptamer with internal tetramethylrhodamine (TMR) label. The OTC binding caused significant changes in FA signals of TMR due to the conformational changes of the TMR-labeled aptamers. We screened out the aptamer with TMR label at the 18th T base, which produced the most sensitive FA response to OTC. Using this TMR-labeled aptamer, we achieved sensitive and selective FA detection of OTC within a short analysis time of 10 min, with a detection limit as low as 0.6 nM (0.28 µg L −1 ). This FA aptamer sensor allowed the detection of spiked OTC in diluted skim milk and lake water samples, showing potential for application in real food and environmental samples.
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ANALYTICAL CHEMISTRY
·
2013
·
IF 5.695
Hui Hou,Xiaojing Bai,Chunyan Xing,Ningyu Gu,Bailin Zhang,Jilin Tang
We present a new method for specific detection of oxytetracycline (OTC) at nanomolar concentrations based on a microfabricated cantilever array. The sensing cantilevers in the array are functionalized with self-assembled monolayers (SAMs) of OTC-specific aptamer, which acts as a recognition molecule for OTC. While the reference cantilevers in the array are functionalized with 6-mercapto-1-hexanol SAMs to eliminate the influence of environmental disturbances. The cantilever sensor shows a good linear relationship between the deflection amplitude and the OTC concentration in the range of 1.0–100 nM. The detection limit of the cantilever array sensor is as low as 0.2 nM, which is comparable to some traditional methods. Other antibiotics such as doxycycline and tetracycline do not cause significant deflection of the cantilevers. It is demonstrated that the cantilever array sensors can be used as a powerful tool to detect drugs with high sensitivity and selectivity.
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Food Analytical Methods
·
2019
·Vol. 12
·期 5
·1121-1127
·
IF 2.413
Wu Zhengzong
A novel aptasensor was described and employed for ultrasensitive determination of the antibacterial agent oxytetracycline (OTC). This method was based on gold nanoparticle (AuNP) tetramers and surface-enhanced Raman spectroscopy (SERS). Under optimal conditions, in the range of 0.01–250 ng/mL, the concentration of OTC exhibited good linear relationship ( R 2 = 0.9948) with changed SERS signal intensity. In addition, the applicability of the novel aptasensor was validated by detecting different concentrations of OTC spiked in milk samples. With its satisfactory performances, this aptasensor is perceived to be a promising candidate for determining OTC in food products. This assay could also be extended for the analysis of other targets (pathogens or harmful substances) in food products by using corresponding DNA sequences.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 14
·13822-13831
·
IF 2.76
Li Yuanzhu,Wang Haijiao,Liu Xiaoxia,Zhao Guangyu,Sun Ying
The dissipation of different residual states of tetracycline antibiotics (TCs) including oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) laboratory microcosm systems was investigated in this study. The residues were fractionated by stepwise extractions into aqueous state (KCl solution extracts), organic state (MeOH extracts), residual state I (citric acid-sodium citrate buffer and ethyl acetate extracts) and residual state II (acetonitrile-EDTA-McIlvaine buffer extracts) for accurate evaluation of TCs pollution. The antibiotics in the aqueous state were hardly detected, whereas the antibiotics in the organic state dissipated relatively fast (not detectable within 15 days after application) and followed simple first-order kinetics (SFOK) ( R
2 from 0.929 to 0.990). While first-order double-exponential decay model (FODED) ( R
2 from 0.840 to 0.999) and availability-adjusted first-order model (AAFO) ( R
2 from 0.939 to 0.999) had a better fit on the dissipation of both residue state I and II than SFOK. TCs in these states were likely sequestered into a dormant undegradable phase since no degradation product was detected during the entire experiment. In addition, the overall 50 % dissipation values (i.e., stability) of the three TCs were OTC > TC > CTC. The TCs tend to dissipate faster in the high water content and organic matter soil.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Fenghua You,Chunyan Hu,Wei Wang,Dan Yu,Baojiang Liu
5CuO/BIO shows rate constants 11.04 and 5.69 times those of CuO and BIO. • The heterojunction accelerates the Cu 2+ /Cu + cycle to efficiently activating persulfate. • LSV demonstrates the electron transfer between the catalyst and PS. • Possible degradation pathways of OTC were inferred using LC-MS and DFT calculations. Persulfate-based advanced oxidation processes (AOPs) are promising for antibiotic degradation, yet efficient and sustainable persulfate (PS) activation remains challenging. Herein, a novel S-scheme heterostructure CuO/BiOIO 3 (CuO/BIO) was synthesized via an in-situ calcination method. Under illumination, the CuO/BIO heterojunction synergistically activated PS, substantially enhancing the photocatalytic degradation of oxytetracycline (OTC). The optimal 5CuO/BIO/PS system achieved an excellent degradation rate constant (k) of 0.0740 min −1 , which is 11.04 and 5.69 times higher than those of CuO/PS and BIO/PS, respectively. X-ray photoelectron spectroscopy (XPS) revealed that the synergistic interaction between CuO and BIO promotes the Cu 2+ /Cu + redox cycle. Photoluminescence (PL) and electrochemical impedance spectroscopy (EIS) demonstrated the superior charge separation capability of the CuO/BIO composite. Linear sweep voltammetry (LSV) confirmed a synergistic effect between photocatalysis and PS activation, which facilitates electron transfer from the catalyst to PS. Additionally, the OTC degradation pathway was elucidated by liquid chromatography-mass spectrometry (LC-MS) identification of intermediates combined with theoretical calculations. Collectively, this study addresses a research gap in the CuO/BIO photocatalytic synergistic PS system and provides both a theoretical basis and experimental evidence for its application in treating antibiotic-containing wastewater. Download: Download high-res image (238KB) Download: Download full-size image
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Ting Ma,Xiaoxuan Wang,Ting Yu,Juan Liu,Zheqiong Yang,Jinlei Xi
Oxytetracycline (OTC), a tetracycline antimicrobial, is one of the antimicrobial drugs frequently used in the aquaculture and livestock industries. Due to its extensive usage and emissions, OTC has been identified as a significant new emerging pollutant (EP) in a number of environments. OTC frequently causes toxic effects on the central nervous system, but it can be challenging to monitor, and it is still unclear how these toxicities are caused. We used bioinformatic analysis techniques to screen for OTC targets and discovered that NMDA receptors are potential targets of OTC neurotoxicity. To confirm this finding, we exposed zebrafish embryos to 5 mg/L OTC-containing rearing water from 2-hour post fertilization (hpf) to 8-day post fertilization (dpf), performed spontaneous movement and light-dark stimulation assays at 6 and 8 dfp, and discovered that OTC inhibited locomotor activity and attenuated anxiety-like responses in zebrafish larvae. Meanwhile, the qPCR and immunofluorescence staining results suggested that OTC inhibited the expression of multiple subtypes of NMDA receptors (grin1a, grin1b, grin2bb, grin2ca) and induced apoptosis in the brains of zebrafish embryos. Simultaneous administration of NMDA, an NMDA receptor agonist, completely antagonized the inhibitory neurobehavioral changes in zebrafish larvae, as well as the downregulation of N-methyl- D -aspartate (NMDA) receptor expression and apoptosis in the embryonic brains caused by OTC exposure. In conclusion, OTC exhibited significant inhibitory neurobehavioral toxicity in zebrafish larvae during early development, which may be dependent on its suppression of NMDA receptor activity and expression. Furthermore, OTC-induced neurodevelopmental toxicity may be associated with NMDA receptor-regulated neuronal apoptosis.
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MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Jinyu Lin,Junchao Qian,Yaping Wang,Yue Yang,Yuxuan Zhang,Jianping Chen,Xingwang Chen,Zhigang Chen
The misuse of antibiotics has led to the emergence of resistant microbial species and boiled over into a serious crisis worldwide. As a member of potent antibiotics, oxytetracycline is frequently detected in water environment. Thus, detecting oxytetracycline in water samples is vital for human health. Herein, we endeavour to build a novel and highly sensitive electrochemical sensor with CeO 2 -Co 3 O 4 QDs@porous carbon nanocomposite, which amplifies the electrode sensor signal for detecting oxytetracycline (OTC). The as-synthesized CeO 2 and Co 3 O 4 quantum dots (QDs) are firmly embedded in the carbon sheets with the size dimension in range of 2–5 nm. The obtained morphological features were evaluated for their electrochemical potential towards oxidation of OTC. The results reveal that this novel electrode exhibits excellent electrocatalytic performance with the linear ranges from 1 × 10 −8 M to 1 × 10 −4 M and a low detection limit of 3.23 × 10 −9 mol L −1 . So, this method paves a new way for constructing electrochemical sensor, which enables a low-cost and effective utilization for the detection of OTC in the agricultural product.
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CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Xiaojing Zhang,Zhao Chen,Nan Zhang,Yongpeng Ma,Yali Song,Yu Li,Hongzhong Zhang
Nanoparticles (NPs) and antibiotics are two emerging pollutants in environment and always simultaneously exist in nitrogen-rich wastewater. In this study, the individual and joint effects of CuO NPs and oxytetracycline (OTC) on partial nitrification (PN) process were investigated in four identical reactors (1 L). Results indicated that 1 mg L −1 CuO NPs and OTC respectively improved the ammonia removal efficiency (ARE) to 68.9% and 76.6%, compared to the 62.9% in control reactor. However, the joint CuO NPs and OTC performed significant suppression on PN and decreased the ARE to 51.0%, which was due to the formative complex of CuO NPs-OTC that disordered the metabolic and inhibited the cell growth. Nitrosomonas shared 38.01% (Control), 39.7% (CuO NPs), 44.29% (OTC) and 39.81% (CuO NPs + OTC), respectively. The joint CuO NPs and OTC induced 6.1 ( tetC ), 7.4 ( tetX ), 3.6 ( tetC ) and 2.9 ( tetM ) times’ amplification of antibiotic resistance genes compared with the blank.
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Analytical Methods
·
2015
·Vol. 7
·期 6
·2585-2593
·
IF 1.821
Congcong Fang,Shijia Wu,Nuo Duan,Shaoliang Dai,Zhouping Wang
A novel sensitive aptasensor was developed for the quantification of oxytetracycline (OTC) in this study. Artificial aptamer-modified magnetic nanoparticles (aptamer-MNPs) were employed as capture probes, and complementary oligonucleotide-modified upconversion nanoparticles (cDNA-UCNPs) were used as signal probes. Then, the probes were hybridized to form poly-network structure MNPs-UCNPs signal probes. Finally, when the target was introduced, the aptamer combined with the priority target and the signal probe was replaced. The proposed method achieved a linear range between 0.05 and 100 ng mL−1, and the limit of detection (LOD) was as low as 0.036 ng mL−1, benefiting largely from labeling with UCNPs, aptamer affinity and magnetic separation. Then, we successfully applied the method to measure OTC in milk samples and validated it by a commercially available enzyme-linked immunosorbent assay (ELISA) method. The results demonstrated that the method possessed high sensitivity and good selectivity for the determination of OTC and is applicable to the determination of OTC in food samples.
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OPTICS AND SPECTROSCOPY
·
2014
·Vol. 117
·期 2
·250-255
·
IF 0.673
Li Jie,Fan Shumin,Li Zhigang,Xie Yuanzhe,Wang Rui,Ge Baoyu,Wu Jing,Wang Ruiyong
The interaction between tetracycline antibiotics and gold nanoparticles was studied. With citrate-coated gold nanoparticles as colorimetric probe, a simple and rapid detection method for doxycycline and oxytetracycline has been developed. This method relies on the distance-dependent optical properties of gold nanoparticles. In weakly acidic buffer medium, doxycycline and oxytetracycline could rapidly induce the aggregation of gold nanoparticles, resulting in red-to-blue (or purple) colour change. The experimental parameters were optimized with regard to pH, the concentration of the gold nanoparticles and the reaction time. Under optimal experimental conditions, the linear range of the colorimetric sensor for doxycycline/oxytetracycline was 0.06–0.66 and 0.59–8.85 μg mL −1 , respectively. The corresponding limit of detection for doxycycline and oxytetracycline was 0.0086 and 0.0838 μg mL −1 , respectively. This assay was sensitive, selective, simple and readily used to detect tetracycline antibiotics in food products.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2017
·
IF 3.133
Tianding Hu,Qingming Jia,Shanchuan He,Shaoyun Shan,Hongying Su,Yunfei Zhi,Lei He
Developing high-performance materials for removal of antibiotics has attracted ever-increasing concerns since variety of harmful antibiotics have been frequently detected in aquatic environments and posed a serious threat to both human health and ecosystems in recent years. In this work, a novel adsorbent (ED-MIL-101) for oxytetracycline antibiotic (OTC) was prepared by grafting ethylenediamine (ED) to Lewis acidic open metal sites in porous metal-organic framework MIL-101. The successful grafting was corroborated via FT-IR spectrum. Compared with the pristine MIL-101, ED-MIL-101 demonstrated a slight decrease in BET surface areas and pore sizes, while the basic frameworks remain invariant. Results for kinetics and thermodynamics showed that the aminofunctionalized adsorbent exhibited a predominant superiority over nongrafted MIL-101 and activated carbon (AC) on the removal of OTC, even though the specific surface area decreased remarkably after grafting. Moreover, ED-MIL-101 can be regenerated by simple washing with ethanol and reusable up to at least the third run with little change in adsorption capacity . Evidence from zeta potential showed that the adsorption of OTC was also driven by electrostatic interaction between the OTC and ED-MIL-101. These results indicate that ED-MIL-101 has great potential in removing OTC contaminant from water.
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