JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 13
·10689-10701
·
IF 2.324
Wang Chenlong,Sun Ting,Zhang Xugang,Chu Mingna,Lv Shixian,Xiang Junyu,Wang JinShuang,Ma Yongjun,Qin Chuanli
It is highly desired to simultaneously introduce active heteroatoms and abundant hierarchical pore structures for enhanced electrochemical performances on carbon materials. Herein, trehalose as a pore-forming agent was added into polyvinylpyrrolidone/melamine formaldedyde resin mixture with high concentrations of nitrogen and oxygen. Then a simple one-step carbonization/activation process was adopted and heteroatom-enriched carbon with hierarchical pores (HPC) was fabricated successfully. HPC/HPC symmetric supercapacitors were assembled using KOH electrolyte. It is clearly demonstrated that due to the pore-forming action of trehalose HPC shows the porous honeycomb, interconnected and worm-like pore structure, which is favorable to enhance the double-layer capacitance. It is confirmed that in our system the three active species of pyridinic nitrogen (N-6), pyrrolic nitrogen (N-5) and quinone type oxygen (O-I) are responsible for the pseudocapacitive behavior. Based on XPS, nitrogen adsorption/desorption isotherms and electrochemical impedance spectroscopy, it is deduced that the ratio-optimized HPC-T30 exhibits high concentration of three active species (8.17 at.%), increased specific area (351.26 m 2 g −1 ) and tuned hierarchical pore structures with substantial micropores (micropore area of 321.68 m 2 g −1 ) and a small amount of mesopores and macropores, which lead to decrease of charge transfer resistance, increase of transfer rate of electrolyte ions in the pores and excellent electrochemical performances. In cyclic voltammetry tests of three-electrode system and galvanostatic charge/discharge tests of two-electrode system, HPC-T30 displays high specific capacitance, 46% and 1.2-time enhancement compared to untreated HPC-T0, respectively. The optimized HPC-T30/HPC-T30 supercapacitor delivers the energy density of 6.69 W h kg −1 in 6 M KOH electrolyte. Furthermore, the supercapacitor shows a capacitance retention of 91.16% up to 6000 cycles and the coulombic efficiency reaches nearly 100% for each charged/discharge cycle, demonstrating its good cyclic stability.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2022
·
IF 5.395
Yan Wang,Shuhui Gao,Kongying Zhu,Lixia Ren,Xiaoyan Yuan
Cryopreservation of red blood cells (RBCs) is imperative for transfusion therapy, while cryoprotectants are essential to protect RBCs from cryoinjury under freezing temperatures. Trehalose has been considered as a biocompatible cryoprotectant that naturally accumulates in organisms to tolerate anhydrobiosis and cryobiosis. Herein, we report a feasible protocol that enables glycerol-free cryopreservation of human RBCs by integration of the synthesized trehalose lipids and dissociative trehalose through ice tuning and membrane stabilization. Typically, in comparison with sucrose monolaurate or trehalose only, trehalose monolaurate was able to protect cell membranes against freeze stress, achieving 96.9 ± 2.0% cryosurvival after incubation and cryopreservation of human RBCs with 0.8 M trehalose. Moreover, there were slight changes in cell morphology and cell functions. It was further confirmed by isothermal titration calorimetry and osmotic fragility tests that the moderate membrane-binding activity of trehalose lipids exerted cell stabilization for high cryosurvival. The aforementioned study is likely to provide an alternative way for glycerol-free cryopreservation of human RBCs and other types of cells.
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yanbin Feng,Nuanqing Wang,Qianqian Cao,Shang Li,Ying Xu,Xinyu Che,Jinfeng Zhao,Chuanlun Yang,Song Xue
Trehalose 6-phosphate (Tre6P) is a critical metabolic signaling molecule in plants, orchestrating diverse biological processes, including stress resistance and photosynthetic efficiency. Recent advancements highlight its promising role in improving crop yield. However, the efficient synthesis of Tre6P in vitro remains a major challenge. Here, we identified a trehalose-6-phosphate phosphorylase WcTre6PPase from Weissella ceti, the enzyme catalyzes the reversible synthesis of Tre6P from β-glucose 1-phosphate (βGlc1P) and glucose 6-phosphate (Glc6P), with a strong catalytic bias for Tre6P synthesis. The crystal structure of WcTre6PPase resolved in complex with βGlc1P reveals critical molecular determinants for substrate recognition and catalytic efficiency. Binding studies demonstrate a higher affinity of WcTre6PPase for βGlc1P by 1.0 ± 0.2 mM compared to Glc6P by 24.3 ± 7.1 mM. Complementary molecular docking and dynamics simulations provide detailed insights into the catalytic mechanism. Subsequently, by integrating WcTre6PPase into a novel multienzyme cascade, comprising maltose phosphorylase, polyphosphate glucokinase, and AMP/ADP-polyphosphate phosphotransferase, and optimizing reaction conditions, we achieved a remarkable Tre6P yield of 90% from maltose. This study provides molecular insights into the function of WcTre6PPase and establishes a prospective platform for Tre6P production for agricultural applications.
研究领域:
结构生物学
·
生物化学
·
植物代谢
·
酶学
·
生物催化
·
农业生物技术
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DOI
PubMed
高影响因子
CRYOBIOLOGY
·
2021
·
IF 2.487
Jin Wang,Xiaodi Shi,Minghao Xiong,Wen-Song Tan,Haibo Cai
The development of an effective cryopreservation method to achieve off-the-shelf and bioactive tissue-engineered constructs (TECs) is important to meet the requirements for clinical applications. The trehalose , a non-permeable cryoprotectant (CPA), has difficulty in penetrating the plasma membranes of mammalian cells and has only been used in combination with other cell penetrating CPA (such as DMSO) to cryopreserve mammalian cells. However, the inherent cytotoxicity of DMSO results in increasing risks with respect to cryopreserved cells. Therefore, in this study, permeable trehalose glycopolymers were synthesised for cryopreservation of TECs. The trehalose glycopolymers exhibited good ice inhibiting activities and biocompatibilities . Furthermore, the viability and function of TECs after cryopreservation with 5.0 wt% S2 were similar to those of the non-cryopreserved TECs. We developed an effective preservation strategy for the off-the-shelf availability of TECs.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Min Liu,Lei Liang,Chaojie Yu,Bingyan Guo,Haitao Zhang,Fanglian Yao,Hong Zhang,Junjie Li
Cell cryopreservation is crucial for biomedicine, and the addition of cryoprotectants (CPAs) to protect cells from cryoinjuries is essential at low temperatures. Trehalose (Tre) has been considered a biocompatible CPA because of its unique physicochemical properties, which could provide cryoprotection to cells during cryopreservation by its interaction with cell membrane phospholipids. Nonetheless, there were few studies on the synthesis of Tre-based CPAs. Here, we reported a feasible solution to further improve the cell cryopreservation efficiency by modifying Tre with sulfoxide functional groups, which enabled dimethyl sulfoxide (DMSO)-free cryopreservation of cells. Compared with unmodified Tre, the sulfoxide-modified Tre (Tre-S) could further protect cells from cryoinjuries. At the optimal CPA concentration (8% Tre-S), the survival rate of L929 cells was up to 88.5% after cryopreservation, and the recovered cells could maintain their normal cell biological behaviors. The differential scanning calorimetry (DSC) and ice recrystallization inhibiting (IRI) tests show that Tre-S has good melting point reduction and IRI activities. Compared with DMSO, the Tre-S had good biocompatibility. This study may provide an alternative method for DMSO-free cell cryopreservation.
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高影响因子
BIOMEDICAL MICRODEVICES
·
2019
·Vol. 21
·期 1
·1-13
·
IF 2.327
Shen Yiren,Du Kun,Zou Lili,Zhou Xiaoming,Lv Rong,Gao Dayong,Qiu Bensheng,Ding Weiping
Freeze-drying is a promising approach for the long-term storage of erythrocytes at room temperature. Studies have shown that trehalose loaded into erythrocytes plays an important role in protecting erythrocytes against freeze-drying damage. Due to the impermeability of the erythrocyte membrane to trehalose, many methods have been developed to load trehalose into erythrocytes. However, these methods usually require multistep manual manipulation and long processing time; the adopted protocols are also diverse and not standardized. Thus, we develop an osmotically-based trehalose-loading microdevice (TLM) to rapidly, continuously, and automatically produce erythrocytes with loaded trehalose. In the TLM, trehalose is loaded through the erythrocyte membrane pores induced by hypotonic shock; then, the trehalose-loaded erythrocytes are rinsed to remove hemoglobin molecules and cell fragments, and the extracellular solution is restored to the isotonic state by integrating a rinsing-recovering design. First, the mixing function and the rinsing-recovering function were confirmed using a fluorescent solution. Then, the performance of the TLM was evaluated under various operating conditions with respect to the loading efficiency of trehalose, the hemolysis rate of erythrocytes ( ϕ ), the recovery rate of hemoglobin in erythrocytes ( φ ), and the separation efficiency of the TLM. Finally, the preliminary study of the freeze-drying of erythrocytes with loaded trehalose was accomplished using the TLM. The results showed that under the designated operating conditions, the loading efficiency for human erythrocytes reached ~21 mM in ~2 min with a ϕ value of ~17% and a φ value of ~74%. This study provides insights into the design of the on-chip loading of trehalose into erythrocytes and promotes the automation of life science studies on biochips.
研究领域:
生物技术
·
细胞生物学
·
微流控
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DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2022
·
IF 5.395
Jianbo Yao,Lingxiao Shen,Zhongrong Chen,Bing Zhang,Gang Zhao
Cryopreservation of red blood cells (RBCs) plays a vital role in preserving rare blood and serologic testing, which is essential for clinical transfusion medicine. The main difficulties of the current cryopreservation technique are the high glycerol concentration and the tedious deglycerolization procedure after thawing. In this study, we explored a microencapsulation method for cryopreservation. RBC–hydrogel microcapsules with a diameter of approximately 2.184 ± 0.061 mm were generated by an electrostatic spraying device. Then, 0.7 M trehalose was used as a cryoprotective agent (CPA), and microcapsules were adhered to a stainless steel grid for liquid nitrogen freezing. The results show that compared with the RBCs frozen by cryovials, the recovery of RBCs after microencapsulation is significantly improved, up to a maximum of more than 85%. Additionally, the washing process can be completed using only 0.9% NaCl. After washing, the RBCs maintained their morphology and adenosine 5′-triphosphate (ATP) levels and met clinical transfusion standards. The microencapsulation method provides a promising, referenceable, and more practical strategy for future clinical transfusion medicine.
研究领域:
生物医学工程
·
血液学
·
输血医学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 30
·23649-23661
·
IF 6.7
Huang Haidong,Li Yangyang,Song Jinsong,Zhao Yuhan,Liu Yanfeng,Li Jianghua,Du Guocheng,Chen Jian,Li Zhendong,Lv Xueqin,Xu Xianhao,Liu Long
Trehalose is a nonreducing disaccharide widely used for
its biomolecule-protective
properties. However, multienzyme cascade production remains limited
by low enzyme expression and suboptimal catalytic performance. To
address this, thermostable maltooligosyltrehalose synthase (TreY)
and trehalohydrolase (TreZ) from Arthrobacter ramosus were individually expressed intracellularly in Bacillus subtilis , and the crude lysates were combined for
trehalose biosynthesis, achieving 281.4 g/L trehalose and a yield
of 0.7 g trehalose/g maltodextrin. Integrated computational screening
identified MalQ-3 from Cyanobacterium stanieri as a suitable 4-α-glucanotransferase for soluble expression.
Subsequent semirational engineering generated MalQ-3-M2 (S54P/V472F),
with enhanced activity and stability associated with improved substrate-pocket
dynamics, thereby facilitating glucan-chain rearrangement and short-chain
reutilization. MalQ-3-M2 was
separately expressed in B. subtilis and incorporated into the crude-lysate cascade, increasing the trehalose
titer to 338 g/L and the yield to 0.85 g trehalose/g maltodextrin.
Overall, this work establishes a scalable B. subtilis platform for efficient trehalose production.
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PubMed
高影响因子
VETERINARY RESEARCH COMMUNICATIONS
·
2025
·Vol. 49
·期 5
·1-10
·
IF 2
Liu Shuang,Qiu Bo,Li Yue,Wang Hai-Peng,Zhong Zhao-Jin,Wang Lin
Glyphosate (Gly) is a globally prevalent herbicide, which poses significant health risks to human and animals via the food chain. The kidney is the primary excretory organ and a site of residual accumulation during Gly exposure. Trehalose (Tre) has been shown to prevent kidney injury induced by various toxicants due to its anti-apoptotic properties. However, the potential protective effect of Tre on Gly-induced kidney damage in poultry remains unclear. Thus, 60 one-day-old healthy Hy-line Brown roosters were randomly divided into three groups ( n = 20), i.e., control group (basal diet without Gly), Gly group (200 mg Gly/kg basal diet), and Gly + Tre group {(200 mg Gly plus 5 g Tre)/kg basal diet}. The experiment lasted for 120 days, and serum and kidney samples were finally collected for respective analysis. Data first showed that Tre treatment can significantly alleviate Gly-induced renal injury in roosters, as evidenced by serum levels of creatinine and blood urea nitrogen as well as renal histopathological observations. Tre also significantly inhibited the elevated abundance of renal injury marker proteins KIM-1 and NGAL induced by Gly. Meanwhile, Tre obviously alleviated Gly-induced ultrastructural abnormalities in the nuclei of renal tissues. Additionally, Tre markedly inhibited Gly-induced apoptotic assays in rooster kidneys, as demonstrated by the results from TUNEL staining and the protein abundance of apoptotic markers cleaved caspase-3 and cleaved PARP. In summary, these findings suggest that Tre exerts its protective effects against Gly-induced kidney damage in roosters via inhibiting apoptosis.
研究领域:
毒理学
·
兽医学
·
分子生物学
·
环境卫生
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PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 18
·15240-15246
·
IF 3.289
Cong-Heng Chen,Ting Yao,Qiang Zhang,Yan-Ming He,Li-Hui Xu,Min Zheng,Guang-Rong Zhou,Yuan Zhang,Hong-Jie Yang,Ping Zhou
Abnormal denaturation and aggregation of human amylin or islet amyloid polypeptide (IAPP) into amyloid fibrils has been implicated in the pathogenesis of type 2 diabetic mellitus. Trehalose, a super-hydrophilic molecule, has been shown to prevent denaturation of biomolecules when they are under environmental stress. In this work, we sought to investigate the effects of trehalose on the fibrillation and aggregation of human IAPP (hIAPP) by using circular dichroism spectrum, thioflavin-T fluorescence spectrum, dynamic light scattering, transmission electronic microscopy, atomic force microscopy and quartz crystal microbalance. We demonstrated that (1) the conformation of hIAPP changed from α-helix to β-sheet, followed by fibrillation and aggregation, (2) a low dose of trehalose (under 100 mM) inhibited or delayed the conformation transition of hIAPP and (3) a high dose (more than 500 mM) induced the conformation transition, and promoted the fibrillation and aggregation of hIAPP. These findings are in agreement with the hypothesis of the water replacement and volume exclusion effect on the proteins. The lower concentration of trehalose could replace the water molecules surrounding the hIAPP, and interact with proteins through hydrogen bonding, leading to a reduction in the protein interaction itself, and therefore inhibiting or delaying the protein fibrillation and aggregation. In contrast, the higher concentration of trehalose might interact with itself to form macromolecular clusters, acting as a crowding agent, leading to the hIAPP molecules being excluded by the trehalose clusters and interacting between each other, and therefore promoting the hIAPP fibrillation and aggregation.
研究领域:
生物化学
·
分子生物学
·
医学
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DOI
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2017
·Vol. 119
·期 9
·1600364
·
IF 2.145
Zhen Wen,Jing Lin,Junqing Su,ZongKun Zheng,Qing Chen,Lidi Chen
The aim of the present work is to evaluate influences of doping trehalose into solid lipid nanoparticles (SLNs) on drug loading. SLNs were prepared by the emulsion-evaporation and low temperature-solidification method, using glyceryl monostearate as a lipid, phosphatidylcholine as a surfactant, trehalose as a modifier and resveratrol as a model drug. As the SLNs were doped by trehalose at a low amount [ m (trehalose): m (lipids) = 1:5], entrapment efficiency and loading capacity were enhanced to (81.37 ± 1.50) and (8.71 ± 0.13)%, respectively. Based on morphology, phase transformation and crystalline structure of SLNs, trehalose was doped into lipid matrix in a non-crystalline state, disrupting the lipid crystallinity and inducing lattice defects.The lipid matrix was crystallized in polymorphic forms composed of α-form and β′-form and β-form, resulting in prevention of drug expulsion from the lipid matrix. The trehalose was interacted with the lipid, facilitating the formation of trehalose/lipid eutectic dopant. The dopant contained lattice defects, allowing for the incorporation of more resveratrol in a amorphous state, contributing to increase of entrapment efficiency and loading capacity. These results confirm stabilizing effects of doping trehalose on improvement of drug loading are attributed to the lipid crystal type and structure of lipid matrix containing lattice defects. Practical applications: Trehalose-doping is an innovative approach to the improvement of loading properties of SLNs. Compared with ordinary SLNs, SLNs doped with trehalose at low amounts exhibit higher drug loading, presenting the potential utility of these nanoparticles as a drug delivery system for hydrophobic drugs. Trehalose modification on improvement of drug loading of SLNs are attributed to the lipid crystal type and structure of lipid matrix containing lattice defects induced by doping trehalose.
研究领域:
药剂学
·
生物医学工程
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shan Su,Xiaohong Liu,Min Zhu,Wen Liu,Jingyi Liu,Yujia Yuan,Fudong Fu,Zhiyong Rao,Jingping Liu,Yanrong Lu,Younan Chen
Nonalcoholic fatty liver disease (NAFLD), characterized by hepatic lipid deposition, is one of the most prevalent chronic metabolic disorders globally, and its pharmaceutical treatments are still limited. Excessive lipid accumulation triggers endoplasmic reticulum (ER) stress and autophagy flux dysfunction, which are important mechanisms for NAFLD. Trehalose (Tre), a natural disaccharide, has been identified to reduce hepatic steatosis and glucose intolerance. However, its underlying mechanisms for NAFLD remain unclear. In this study, a high-fat-diet (HFD)-induced mouse NAFLD model and a saturated fatty acid palmitic acid (PA)-stimulated cell model were constructed. The results indicated that Tre supplementation ameliorated hepatocyte lipid deposition in vitro, as well as hepatic steatosis and hyperlipidemia in vivo. Mechanistically, Tre alleviated both autophagy flux dysfunction and endoplasmic reticulum (ER) stress. Under the stimulation of HFD or PA, Tre remarkably increased the expression and nucleic translocation of the lysosomal master protein transcription factor EB (TFEB), while decreasing the accumulation of p62 and also decreasing the ER stress markers (inositol-requiring enzyme 1 (IRE1α), XBP-1, CHOP, and BIP). Similar results were observed in an ER stressor tunicamycin (TM)-induced in vivo and in vitro models. In addition, the transcriptomic analysis of NAFLD patients revealed significant differences in ER stress-related and autophagy-related biomarkers, including TFEB, ATG7, IRE1α, and CHOP. Molecular docking results demonstrated a strong affinity between Tre and both IRE1α and TFEB. Overall, Tre protected hepatocytes from lipotoxicity-related ER stress and autophagy dysfunction, and its regulatory effect on the IRE1α–TFEB signaling pathway may be a critical mechanism. These findings suggest that Tre, as a bioactive substance with significant medicinal potential, holds considerable promise for drug development and clinical application in treating NAFLD.
研究领域:
药理学
·
分子生物学
·
代谢紊乱
·
肝病学
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DOI
PubMed
高影响因子
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