Non-specific binding to Leukocytes is a well-known challenge in flow cytometry. Though the specific mechanism of non-specific binding is not known, the binding of cyanine acceptor fluorophores to CD64 has been implicated in the literature. Mouse Fc Receptor Blocking Solution Plus not only specifically blocks non-specific antibody binding to CD16/CD32 (low-affinity IgG Fc receptors III and II), preventing non-specific binding of immunoglobulins to Fc receptors, but also effectively inhibits non-specific staining by various PE, PerCP, and APC tandem dyes on leukocytes. Compared to using anti-mouse CD16/32 mouse Fc receptor blocker alone, the new product demonstrates superior blocking performance against non-specific signals on Cyanine dyes.
This buffer contains specialized Rat IgG containing 0.05% ProClin 300. It is not recommended to be used for staining Rat IgG. Handle as biohazard agent.
Mouse Fc Receptor Blocking Solution Plus blocks both clone P90A23 and 2.4G2 also raised against mouse CD16/32.
Cyanine dye blocking solution | Blocking buffer for Flow | Blocking Reagent | Fc Receptor Blocker | FcR Blocking Reagent | Fc Receptor Blocking Solution
规格或纯度
无菌过滤, BioReagent, 10 μL/test
稳定性与储存
Store at 2-8°C for 12 months. When stored at 2°C to 8°C, the presence of serum in this product may cause aggregation of various proteins and lipoproteins (such as cold agglutinins, fibrinogen, vitronectin, etc.), forming visible precipitate or cloudiness.
英文名称
Mouse Fc Receptor Blocking Solution Plus
储存条件
2-8°C储存,避光
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
图片
Mouse Fc Receptor Blocking Solution Plus (M1374079) - Flow Cytometry Mouse Fc Receptor Blocking Solution Plus (M1374079) reduces nonspecific staining but preserves specific staining of mouse bone marrow cells (BMC) for CD11b. Mouse BMC were either not preincubated (Left Plots) or were preincubated (Middle Plots) with Mouse FcR Blocking Reagent (Ab219990), or were preincubated (Right Plots) with Mouse Fc Receptor Blocking Solution Plus as indicated. The cells were then stained with Rat IgG (PE-Cy5) (Ab190072) and recombinant CD11b Antibody (AF405) (Ab213132). Two-dimensional pseudocolor plots of CD11b vs Rat IgG show that the Mouse Fc Receptor Blocking Solution Plus reduces non-specific binding compared to staining without any blocking reagent and with Mouse FcR Blocking Reagent.
Mouse Fc Receptor Blocking Solution Plus (M1374079) - Flow Cytometry Mouse Fc Receptor Blocking Solution Plus (M1374079) reduces nonspecific staining but preserves specific staining of mouse splenocytes for CD45R. Mouse splenocytes were either not preincubated (Left Plots) or were preincubated (Middle Plots) with Mouse FcR Blocking Reagent (Ab219990), or were preincubated (Right Plots) with Mouse Fc Receptor Blocking Solution Plus as indicated. The cells were then stained with Rat IgG (PE-Cy5) (Ab190072) and CD45R Rat mAb (FITC) (Ab191667). Two-dimensional pseudocolor plots of CD45R vs Rat IgG show that the Mouse Fc Receptor Blocking Solution Plus reduces non-specific binding compared to staining without any blocking reagent and with Mouse FcR Blocking Reagent.
应用
应用名称
稀释比例
Flow Cytometry
10 μL/test. It is recommended to add 10 µL of Mouse Fc Receptor Blocking Solution Plus pe
ZJ25F0825779
危险品化学品经营许可证（带存储）