Mtb-cyt-bd oxidase-IN-3 (compound 1u) is a Mycobacterium tuberculosis cytochrome bd oxidase ( Mtb cyt-bd oxidase ) inhibitor with an IC 50 value of 0.36 μM. Mtb-cyt-bd oxidase-IN-3 inhibits the growth of Mycobacterium tuberculosis (MIC=32 μM). Mtb-cyt-bd oxidase-IN-3 can be used in tuberculosis research.