MtTMPK-IN-5 (compound 17) is a potent M. tuberculosis thymidylate kinase ( Mtb TMPK) inhibitor with an IC 50 value of 34 μM. MtTMPK-IN-5 combines favorable enzyme inhibitory activity with significant activity against M. tuberculosis (MIC = 12.5 μM). MtTMPK-IN-5 can be used for researching tuberculosis.