m3227G(5)ppp(5')m6Am is a specific RNA molecule structure, which is composed of a 3227-methylguanine (m3227G) cap , a triphosphate group and a N6,2'-O-dimethyladenosine. m6Am is a reversible modification located on the first coding nucleotide near the 5' cap of mRNA , that can affect the stability of mRNA.