Multitarget AD inhibitor-2 (Compound VN-19) is a multitargeting inhibitor acetylcholinesterase ( AChE , IC 50 =0.14 μM), butyrylcholinesterase ( BChE , IC 50 =11.6 μM), monoamine oxidase B ( MAO B , IC 50 =0.45 μM). Multitarget AD inhibitor-2 inhibits self-induced aggregation of amyloid beta protein Aβ1-42 (inhibition rate is 47.3% at 20 μM), and downregulates the level of ROS in SH-SY5Y (80 inhibition rate at 25 μM). Multitarget AD inhibitor-2 ameliorates the cognitive decline in Scopolamine -induced Alzheimer’s Disease zebrafish models.