BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
镁是多种酶的辅助因子，存在于软组织和骨中，二者的分布大致相等，镁的代谢机制尚不清楚，镁增加会导致肌张力减弱，镁减少见于甲状旁腺功能减退、慢性肾衰竭等。
检测原理：溶液中镁离子在碱性条件下能与甲基麝香草酚蓝（MTB）结合，生成蓝紫色的复合物，加入钙离子螯合剂，去除钙离子背景干扰，通过分光光度计检测600nm处吸光度，根据公式计算出镁含量。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
| M1506762 | Component | 100T | Storage |
| M1506762A | 镁标准 (0.823 mmol/L) | 1 mL | 2-8℃ |
| M1506762B | Mg Assay Buffer | 100 mL | 2-8℃ |
| M1506762C | MTB显色液 | 100 mL | 2-8℃. Store in the dark. |
| M1506762D | ddH₂O | 100 mL | RT |
自备仪器和试剂
离心管或试管、比色杯、分光光度计
实验流程
1、制备样品
1.1 血浆、血清样品
血浆、血清按照常规方法制备，可以直接用于该试剂盒的测定，-20℃冻存，用于Mg的检测。
1.2 细胞或组织样品
取恰当细胞或组织进行匀浆，低速离心取上清，-20℃冻存，用于Mg的检测。
1.3 高浓度样品
如果样品中含有较高浓度的Mg，可以使用ddH₂O稀释，不宜使用普通蒸馏水稀释。
注：样品准备完毕后可以用BCA蛋白浓度测定试剂盒测定蛋白浓度，以便于后续计算单位蛋白重量组织或细胞内的Mg含量。推荐使用阿拉丁B665595 BCA蛋白定量试剂盒或R1491648 即用型BCA蛋白定量试剂盒。
2、配制Mg显色工作液
临用前，取Mg Assay Buffer和MTB显色液等量混合，即配即用；4℃避光保存，不宜久置。3、Mg加样
选用经稀盐酸处理及去离子水清洁的干燥试管或者一次性无菌聚乙烯离心管，按照下表设置空白管、标准管、测定管，溶液应按照顺序依次加入，并注意避免产生气泡。如果样品中的镁离子含量过高，可以减少样品用量或适当稀释后再进行测定，样品的检测最好能设置平行管。
| 加入物 (ml) | 空白管 | 标准管 | 测定管 |
| ddH₂O | 0.2 | — | — |
| 镁标准 (0.823mmol/L) | — | 0.2 | — |
| 待测样品 | — | — | 0.2 |
| Mg显色工作液 | 1.8 | 1.8 | 1.8 |
4、Mg测定
混匀，室温静置5min，溶液颜色呈绿色至淡蓝紫色，以空白管调零，比色杯光径1cm，分光光度计测定标准管、测定管600nm处吸光度（即为A标准、A测定）。
5、结果计算
血清、血浆中镁 (mmol/L)=(A测定/A标准)×0.823
组织中镁浓度 (mmol/mg)=(A测定/A标准)×0.823/(c×1000)
参数说明：
A测定：测定管的吸光度
A标准：标准管的吸光度
c：待测样品蛋白浓度，mg/mL
单位换算：mg/dl=mmol/L/0.411
参考区间：成年健康人血清镁浓度：0.67~1.04mmol/L (1.64~2.52mg/dl)
注意事项
1、溶血样本对检测有干扰，尽量避免采用溶血样本。
2、本法能够用于自动生化分析仪终点检测法。
3、在该试剂盒条件下，建议待测样品中镁离子浓度应大于0.08mmol/L为宜，否则有可能造成检测误差。
4、镁标准在0.823mmol/L时，通过分光光度计测定吸光度多在0.2~0.4之间。根据测定经验，样品浓度小于0.1mmol/L或高于1.6mmol/L，测定结果有偏差。
5、如果样品浓度过高，应用ddH₂O稀释后重测，结果乘以稀释倍数。
Magnesium acts as a cofactor for numerous enzymes and is present in both soft tissues and bone, with roughly equal distribution between the two. The metabolic regulation of magnesium remains incompletely understood. Elevated magnesium levels can lead to reduced muscle tone, while decreased levels are associated with conditions such as hypoparathyroidism and chronic renal failure.
Detection Principle
In an alkaline solution, magnesium ions bind with Methyl Thymol Blue (MTB) to form a blue-violet complex. The addition of a calcium ion chelator eliminates background interference from calcium ions. The absorbance of this complex is measured at 600 nm using a spectrophotometer, and the magnesium content is calculated based on a formula. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| M1506762 | Component | 100T | Storage |
| M1506762A | Magnesium Standard (0.823 mmol/L) | 1 mL | 2-8℃ |
| M1506762B | Mg Assay Buffer | 100 mL | 2-8℃ |
| M1506762C | MTB Chromogenic Solution | 100 mL | 2-8℃. Store in the dark. |
| M1506762D | ddH₂O | 100 mL | RT |
Materials Required but Not Provided
Centrifuge tubes or test tubes, Cuvettes, Spectrophotometer
Experimental Procedure
1. Sample Preparation
1.1 Plasma/Serum Samples
Plasma or serum samples prepared by standard methods can be used directly for assay with this kit. Store at -20°C for Mg detection.
1.2 Cell or Tissue Samples
Homogenize an appropriate amount of cells or tissue. Centrifuge at low speed to collect the supernatant. Store at -20°C for Mg detection.
1.3 High Concentration Samples
If the sample contains a high concentration of Mg, dilute with the provided ddH₂O. The use of ordinary distilled water for dilution is not recommended.
Note: After sample preparation, the protein concentration can be determined using a BCA protein assay kit to facilitate subsequent calculation of Mg content per unit protein weight in tissues or cells. The Aladdin B665595 BCA Protein Quantification Kit or R1491648 Ready-to-Use BCA Protein Quantification Kit is recommended.
2. Preparation of Mg Chromogenic Working Solution
Immediately before use, mix equal volumes of Mg Assay Buffer and MTB Chromogenic Solution. Prepare fresh for each use. If necessary, the working solution can be stored at 4°C protected from light for short periods, but prolonged storage is not recommended.
3. Mg Assay Setup
Use dry test tubes cleaned with dilute hydrochloric acid and rinsed with deionized water, or disposable sterile polyethylene centrifuge tubes. Set up Blank, Standard, and Test tubes according to the table below. Add solutions in the specified order, avoiding bubble formation. If the magnesium ion concentration in the sample is too high, reduce the sample volume or dilute appropriately before assay. Running sample duplicates is recommended.
| Reagent (mL) | Blank Tube | Standard Tube | Test Tube |
| ddH₂O | 0.2 | — | — |
| Magnesium Standard (0.823 mmol/L) | — | 0.2 | — |
| Test Sample | — | — | 0.2 |
| Mg Chromogenic Working Solution | 1.8 | 1.8 | 1.8 |
4. Mg Measurement
Mix well and incubate at room temperature for 5 minutes. The solution color develops from green to light blue-violet. Using the Blank tube to zero the instrument, measure the absorbance of the Standard and Test tubes at 600 nm using a cuvette with a 1 cm light path (recorded as Astandard and Atest).
5. Calculation of Results
Serum/Plasma Magnesium:
Magnesium (mmol/L) = (Atest / Astandard) × 0.823
Tissue Magnesium:
Magnesium (mmol/mg prot) = (Atest / Astandard) × 0.823 / (c × 1000)
Parameter Description:
Atest: Absorbance of the test tube
Astandard: Absorbance of the standard tube
c: Protein concentration of the test sample, mg/mL
Unit Conversion: mg/dL = mmol/L / 0.411
Reference Interval: Serum magnesium concentration in healthy adults: 0.67 ~ 1.04 mmol/L (1.64 ~ 2.52 mg/dL)
Precautions
Hemolyzed samples interfere with the assay; avoid using them whenever possible.
This method can be adapted for automated biochemical analyzers using an endpoint detection method.
Under the conditions of this kit, the magnesium ion concentration in the test sample should preferably be greater than 0.08 mmol/L to avoid potential detection errors.
The absorbance of the 0.823 mmol/L Magnesium Standard, when measured by spectrophotometer, typically falls between 0.2 and 0.4. Empirical data suggests results may deviate for sample concentrations below 0.1 mmol/L or above 1.6 mmol/L.
If the sample concentration is too high, dilute with ddH₂O and re-assay, multiplying the result by the dilution factor.
Use reagents promptly after opening to prevent potential effects on subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 H2629005 |
分析证书 | M1506762 |
| E2606004 |
分析证书 | M1506762 |
| ZJ26F0131810 |
分析证书 | M1506762 |
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