BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
镁是多种酶的辅助因子，存在于软组织和骨中，二者的分布大致相等，其代谢机制尚不清楚，镁增加会导致肌张力减弱，镁减少见于甲状旁腺功能减退、慢性肾衰竭等。
| M1506768 | Component | 100T | 200T | Storage |
| M1506768A | 镁标准 (0.823 mmol/L) | 1 mL | 1 mL | 2-8℃ |
| M1506768B | Calmagite显色液 | 3 mL | 6 mL | 2-8℃. Store in the dark. |
| M1506768C | Mg Assay Buffer | 3 mL | 6 mL | 2-8℃. Store in the dark. |
| M1506768D | Calmagite基液 | 1 mL | 1 mL | 2-8℃ |
自备仪器和试剂
1、去离子水、稀氢氧化钾溶液或稀盐酸溶液
实验流程
1、制备样品
1.1 血浆、血清样品
血浆、血清按照常规方法制备，可以直接用于该试剂盒的测定，-20℃冻存，用于Mg的检测。
1.2 细胞或组织样品
取恰当细胞或组织进行匀浆，低速离心取上清，-20℃冻存，用于Mg的检测。
1.3 高浓度样品
如果样品中含有较高浓度的Mg，可以使用ddH₂O稀释，不宜使用普通蒸馏水稀释。
注：样品准备完毕后可以用BCA蛋白浓度测定试剂盒测定蛋白浓度，以便于后续计算单位蛋白重量组织或细胞内的Mg含量。推荐使用阿拉丁B665595 BCA蛋白定量试剂盒或R1491648 即用型BCA蛋白定量试剂盒。
3、Mg加样
选用经稀盐酸处理及去离子水清洁的96孔板，按照下表设置空白孔、标准孔、测定孔，溶液应按照顺序依次加入，并注意避免产生气泡；如果样品中的镁离子含量过高，可以减少样品用量或适当稀释后再进行测定，样品的检测最好能设置平行孔。
| 加入物 (μl) | 空白孔 | 标准孔 | 测定孔 |
| ddH₂O | 5.6 | — | — |
| 镁标准 (0.823mmol/L) | — | 5.6 | — |
| 待测样品 | — | — | 5.6 |
| Mg显色工作液 | 280 | 280 | 280 |
4、Mg测定
混匀，以空白孔调零，酶标仪测定标准孔、测定孔510nm处吸光度（记为A标准、A测定）。
5、结果计算
血清、血浆中镁(mmol/L)=(A测定/A标准)×0.823
组织中镁(mmol/mg)=(A测定/A标准)×0.823/c
参数说明：
A测定：测定孔的吸光度
A标准：标准孔的吸光度
c：待测样品蛋白浓度，mg/L
单位换算：mg/dl=mmol/L/0.411
参考区间：成年健康人血清镁浓度：0.7~1.1mmol/L
注意事项
1、溶血样品对检测有干扰，尽量避免采用溶血样品。
2、试剂开封后请尽快使用，以防影响后续实验效果。
3、脂血样本对检测也有干扰，样本应去脂处理后再进检测。
4、在该试剂盒条件下，待测样品中镁离子浓度应大于0.05mmol/L、小于0.823mmol/L为宜，镁离子浓度高于0.6mmol/L以上建议用去离子稀释后再测定，否则有可能造成检测误差。
5、本法能够用于自动生化分析仪终点检测法。
6、如果样品浓度过高，应用ddH₂O稀释后重测，结果乘以稀释倍数。
7、注意避免Mg²⁺的污染，以免影响检测结果。
8、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Magnesium serves as a cofactor for numerous enzymes and is present in both soft tissues and bone, with roughly equal distribution between the two. Its metabolic mechanisms are not yet fully understood. Elevated magnesium levels can lead to reduced muscle tone, while decreased levels are observed in conditions such as hypoparathyroidism and chronic renal failure.
Detection Principle
In an alkaline solution, magnesium ions bind with the Calmagite reagent to form a magenta-red complex. The color intensity is proportional to the magnesium ion concentration. The absorbance is measured at 510 nm using a microplate reader, and the magnesium content is calculated based on a formula. The solution contains the calcium chelator EGTA to eliminate calcium interference. The inclusion of a surfactant stabilizes the protein colloid, allowing for direct magnesium measurement in serum without protein removal. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.
| M1506768 | Component | 100T | 200T | Storage |
| M1506768A | Magnesium Standard (0.823 mmol/L) | 1 mL | 1 mL | 2-8℃ |
| M1506768B | Calmagite Chromogenic Solution | 3 mL | 6 mL | 2-8℃. Store in the dark. |
| M1506768C | Mg Assay Buffer | 3 mL | 6 mL | 2-8℃. Store in the dark. |
| M1506768D | Calmagite Base Solution | 1 mL | 1 mL | 2-8℃ |
Materials Required but Not Provided
Deionized water, dilute potassium hydroxide solution, or dilute hydrochloric acid solution
Centrifuge tubes or test tubes, centrifuge, 96-well plate, microplate reader
Experimental Procedure
1. Sample Preparation
1.1 Plasma/Serum Samples
Plasma or serum samples prepared by standard methods can be used directly. Store at -20°C for Mg detection.
1.2 Cell or Tissue Samples
Homogenize an appropriate amount of cells or tissue. Centrifuge at low speed to collect the supernatant. Store at -20°C for Mg detection.
1.3 High Concentration Samples
If the sample contains a high Mg concentration, dilute with ddH₂O. The use of ordinary distilled water is not recommended.
Note: After preparation, determine the protein concentration using a BCA protein assay kit to calculate the Mg content per unit protein weight in tissues or cells. The Aladdin B665595 BCA Protein Quantification Kit or R1491648 Ready-to-Use BCA Protein Quantification Kit is recommended.
2. Preparation of Mg Chromogenic Working Solution
Immediately before use, mix the components in the ratio: Calmagite Chromogenic Solution : Mg Assay Buffer : Calmagite Base Solution : Deionized Water = 10 : 10 : 1 : 80. Adjust the pH to 11.5 ± 0.2 using 1M potassium hydroxide solution. This is the Mg Chromogenic Working Solution. Store at 4°C protected from light; stable for 2 weeks.
3. Mg Assay Setup
Use a 96-well plate cleaned with dilute hydrochloric acid and rinsed with deionized water. Set up Blank, Standard, and Test wells according to the table below. Add reagents in the specified order, avoiding bubbles. For high Mg concentrations, reduce the sample volume or dilute appropriately. Running duplicates is recommended.
| Reagent (μL) | Blank Well | Standard Well | Test Well |
| ddH₂O | 5.6 | — | — |
| Magnesium Standard (0.823 mmol/L) | — | 5.6 | — |
| Test Sample | — | — | 5.6 |
| Mg Chromogenic Working Solution | 280 | 280 | 280 |
4. Mg Measurement
Mix well. Using the Blank well to zero the instrument, measure the absorbance of the Standard and Test wells at 510 nm (recorded as 朱传侠A<sub>standard</sub> and A<sub>test</sub>).
V. Calculation of Results
Serum/Plasma Magnesium:
Magnesium (mmol/L) = (Atest / Astandard) × 0.823
Tissue Magnesium:
Magnesium (mmol/mg prot) = (Atest / Astandard) × 0.823 / c
Parameter Description:
Atest: Absorbance of the test well
Astandard: Absorbance of the standard well
c: Protein concentration of the test sample, mg/L
Unit Conversion: mg/dL = mmol/L / 0.411
Reference Interval: Serum magnesium concentration in healthy adults: 0.7 ~ 1.1 mmol/L
Precautions
Hemolyzed samples interfere with the assay; avoid their use whenever possible.
Use reagents promptly after opening to prevent potential effects on subsequent experiments.
Lipemic samples also interfere; delipidate samples before assay.
Under this kit's conditions, the magnesium ion concentration in the test sample should ideally be greater than 0.05 mmol/L and less than 0.823 mmol/L. For concentrations above 0.6 mmol/L, dilute with deionized water before measurement to avoid potential errors.
This method can be adapted for automated biochemical analyzers using an endpoint detection method.
If the sample concentration is too high, dilute with ddH₂O and re-assay, multiplying the result by the dilution factor.
Avoid contamination with Mg²⁺ ions, as this can affect the detection results.
For your safety and health, wear a lab coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131309 |
分析证书 | M1506768 |
危险品化学品经营许可证（带存储）