计算溶液所需的质量、体积或浓度。
甲基绿染色液（2%）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
甲基绿染色液（2%）是一种组织或细胞染色时常用的可以把细胞核染成绿色的染色液。甲基绿可以和细胞核中的DNA结合，从而产生细胞核染色。本染色液可以和免疫荧光染色或免疫组化染色配合使用。一方面可以在本甲基绿染色液染色后进行免疫荧光染色或其它染料的染色，另一方面也可以在免疫组化染色后再进行甲基绿复染。
10ml染色液可以染色20个样本。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
使用说明
1、样品处理
1.1 对于石蜡切片
- 二甲苯或脱蜡透明液中脱蜡5～10min。
- 更换新鲜的二甲苯或脱蜡透明液，再脱蜡5～10min。
- 无水乙醇5min。
- 90%乙醇2min。
- 70%乙醇2min。
- 蒸馏水2min。
1.2 对于冰冻切片
- 蒸馏水2min。
1.3 对于培养细胞
- 用4%多聚甲醛固定10min以上。
- 蒸馏水洗涤2min。
- 更换新鲜的蒸馏水，再洗涤2min。
2、甲基绿染色
- 对于上述处理好的样本，用甲基绿染色液（2%）染色5～10min。
- 用蒸馏水冲洗2次，此时样本呈蓝色。
- 95%乙醇处理5s。
- 此时，如果需要直接观察，可以用70%乙醇洗涤2次。如需脱水、透明后封片按后续步骤进行，70%乙醇洗涤后仍可按照后续步骤进行脱水、透明和封片处理。
3、组织切片染色
- 95%乙醇脱水2min。
- 更换新鲜的95%乙醇再脱水2min。
- 二甲苯或脱蜡透明液透明5min。
- 二甲苯或脱蜡透明液再透明5min。
- 中性树胶或其它封片剂封片。
- 显微镜下观察，细胞核呈绿色或蓝绿色。
4、荧光染色
- 如果进行免疫荧光染色，在甲基绿染色液染色后，70％乙醇洗涤2次，每次2min。
- 95%乙醇脱水2min。
- PBS或生理盐水或TBS等用于免疫染色或荧光染料染色的溶液浸泡5min。
- 进行免疫荧光染色或其它荧光染料的染色。
染色结果
细胞核呈绿色或蓝绿色。
注意事项
1、需自备4%多聚甲醛、蒸馏水、系列乙醇、二甲苯等。
2、第一次使用本试剂盒时建议先取1-2个样品做预实验。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Methyl Green Staining Solution (2%) is a commonly used staining solution in tissue or cell staining that colors the nucleus green. Methyl Green binds to DNA in the nucleus, resulting in nuclear staining. This staining solution can be used in combination with immunofluorescence staining or immunohistochemical staining. On one hand, immunofluorescence staining or staining with other dyes can be performed after staining with this Methyl Green solution; on the other hand, Methyl Green can also be used as a counterstain after immunohistochemical staining.
10 mL of staining solution can stain approximately 20 samples. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Instructions for Use
1. Sample Processing
1.1 For Paraffin Sections
Deparaffinize in xylene or dewaxing clearing solution for 5–10 minutes.
Replace with fresh xylene or dewaxing clearing solution and deparaffinize for another 5–10 minutes.
Absolute ethanol for 5 minutes.
90% ethanol for 2 minutes.
70% ethanol for 2 minutes.
Distilled water for 2 minutes.
1.2 For Frozen Sections
Distilled water for 2 minutes.
1.3 For Cultured Cells
Fix with 4% paraformaldehyde for at least 10 minutes.
Wash with distilled water for 2 minutes.
Replace with fresh distilled water and wash for another 2 minutes.
2. Methyl Green Staining
For the processed samples above, stain with Methyl Green Staining Solution (2%) for 5–10 minutes.
Rinse twice with distilled water. The samples will appear blue at this stage.
Treat with 95% ethanol for 5 seconds.
At this point, if direct observation is desired, wash twice with 70% ethanol. If dehydration, clearing, and mounting are required, proceed with the following steps. Even after washing with 70% ethanol, dehydration, clearing, and mounting can still be performed.
3. Staining of Tissue Sections
Dehydrate with 95% ethanol for 2 minutes.
Replace with fresh 95% ethanol and dehydrate for another 2 minutes.
Clear with xylene or dewaxing clearing solution for 5 minutes.
Clear again with xylene or dewaxing clearing solution for 5 minutes.
Mount with neutral balsam or other mounting medium.
Observe under a microscope: nuclei appear green or bluish‑green.
4. Fluorescence Staining
If performing immunofluorescence staining, after staining with Methyl Green Staining Solution, wash twice with 70% ethanol, 2 minutes each time.
Dehydrate with 95% ethanol for 2 minutes.
Soak in PBS, physiological saline, TBS, or other solutions used for immunostaining or fluorescent dye staining for 5 minutes.
Proceed with immunofluorescence staining or other fluorescent dye staining.
Staining Results
Nuclei appear green or bluish‑green.
Notes
Users need to prepare 4% paraformaldehyde, distilled water, graded ethanol series, xylene, etc., themselves.
When using this kit for the first time, it is recommended to perform a preliminary experiment with 1–2 samples.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
This product is restricted to scientific research by professionals and must not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals. It should not be stored in ordinary residential areas.
For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
英文别名
Methyl Green Stain
规格或纯度
BioReagent,生物染色剂,用于显微镜,2%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Methyl Green Staining Solution (2%)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838284 |
分析证书 | M1508541 |
| ZJ26F0131442 |
分析证书 | M1508541 |
技术文档和文章
溶液计算器
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