BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用双抗体夹心 ELISA 法检测样品中小鼠 IgG1 的浓度。小鼠 IgG1 捕获抗体已经预包被于 酶标板上，当加入样品或标准品时，其中的小鼠 IgG1 会与捕获抗体结合，而其它游离成分则会通过洗涤 被除去。接着，再加入 HRP 标记的小鼠 IgG1 抗体后，抗小鼠 IgG1 抗体与小鼠 IgG1 结合，形成夹心的免 疫复合物，其它游离成分则通过洗涤被除去。化无色的 显色剂氧化生成蓝色物质，而后加入终止液，最终产物呈黄色。通过酶标仪检测，读取450nm处的OD值，小鼠 IgG1 浓度与OD450值之间呈正比，通过检测标准品绘制标准曲线，对照未知样品中OD值，即 可计算出样品中小鼠 IgG1 的浓度。
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of mouse IgG1 concentration in samples. The mouse IgG1 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, the mouse IgG1 present therein binds to the immobilized capture antibody. Unbound components are then removed through a washing step. Next, a horseradish peroxidase (HRP)-conjugated detection antibody specific for mouse IgG1 is added. This antibody binds to the captured mouse IgG1, forming a sandwiched immunocomplex. Following another wash to remove any unbound material, a colorimetric substrate solution (typically TMB) is added. In the presence of the immunocomplex, the conjugated HRP catalyzes the oxidation of the colorless substrate, producing a blue-colored product. The enzymatic reaction is terminated by adding a stop solution (usually an acid), which changes the color from blue to yellow. The absorbance, or Optical Density (OD), of each well is immediately measured at a wavelength of 450nm using a microplate reader.The concentration of mouse IgG1 in the sample is directly proportional to the OD450 value. A standard curve is constructed by plotting the OD450 values of known mouse IgG1 standard concentrations. The concentration of mouse IgG1 in unknown samples is then determined by interpolating their OD450 values from this standard curve.
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