计算溶液所需的质量、体积或浓度。
超氧阴离子等活性氧在生物体内具有免疫和信号传导的作用,但积累过多会对细胞膜及生物大分子产生破坏作用,导致机体细胞和组织代谢异常,从而引起多种疾病。 本试剂盒检测原理是超氧阴离子与盐酸羟胺反应生成NO₂⁻,NO₂⁻与格里斯试剂反应,生成红色的偶氮化合物,测定其在 540 nm 处的吸光度值即可计算样本中超氧阴离子的含量。
Superoxide anions and other reactive oxygen species play roles in immunity and signal transduction within organisms. However, excessive accumulation can damage cell membranes and biological macromolecules, leading to abnormal metabolism in cells and tissues, thereby causing various diseases. The detection principle of this kit is that superoxide anions react with hydroxylamine hydrochloride to generate NO₂⁻. NO₂⁻ then reacts with the Griess reagent to form a red azo compound. The content of superoxide anions in the sample can be calculated by measuring its absorbance at 540 nm.