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MCS广泛存在于动物、植物、微生物和培养细胞的线粒体基质中，与柠檬酸合酶（CS）共同参与三羧酸循环的调节。 测定原理：MCS催化丙酰CoA和草酰乙酸产生甲基柠檬酰辅酶A，进一步水解产生甲基柠檬酸，该反应促使无色的DTNB转变成黄色的TNB，在 412nm处有特征吸光值。
Methylmalonyl-CoA synthase is widely present in the mitochondrial matrix of animals, plants, microorganisms, and cultured cells. It works in conjunction with citrate synthase (CS) to participate in the regulation of the tricarboxylic acid (TCA) cycle, primarily involved in the metabolism of propionate. Assay Principle: MCS catalyzes the condensation of propionyl-CoA and oxaloacetate to form methylmalonyl-CoA. The methylmalonyl-CoA is subsequently hydrolyzed to yield methylcitrate. This series of reactions drives the conversion of the colorless compound DTNB into the yellow-colored product TNB, which exhibits a characteristic absorbance at 412 nm.
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